FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_H3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_H3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3910951
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATATACCGGAACCAAGGGAGACTGAGTGGAGGGGTGAACAGACCCAG530651.356831113455525No Hit
CTATTAATGAATCTGGGCTCCTACAGACCCTTGTGGGGAATGCTTCCTCT524881.3420776685772846No Hit
GAATAATATACCGGAACCAAGGGAGACTGAGTGGAGGGGTGAACAGACCC453451.159436668984091No Hit
GACTGGGACCGTCACCTCCCTCTCTCTGCACGCTCCACCTCTATTAATGA381400.975210377220272No Hit
CTCCACCTCTATTAATGAATCTGGGCTCCTACAGACCCTTGTGGGGAATG312630.7993707924236331No Hit
CTACAGACCCTTGTGGGGAATGCTTCCTCTCCTCTCCTTTGCCTCCCTCT274550.7020031700729567No Hit
ATCATAGAGGGAGGCAAAGGAGAGGAGAGGAAGCATTCCCCACAAGGGTC233400.5967857945548282No Hit
GGGGAATAATATACCGGAACCAAGGGAGACTGAGTGGAGGGGTGAACAGA221300.5658470279990724No Hit
TCATAGAGGGAGGCAAAGGAGAGGAGAGGAAGCATTCCCCACAAGGGTCT217760.5567955210893718No Hit
CCTACAGACCCTTGTGGGGAATGCTTCCTCTCCTCTCCTTTGCCTCCCTC201040.5140437709396002No Hit
CTCTATGATTACTTACTTCTGGGTCTGTTCACCCCTCCACTCAGTCTCCC194610.4976027569765001No Hit
CTCTCTGCACGCTCCACCTCTATTAATGAATCTGGGCTCCTACAGACCCT192760.492872449693182No Hit
AAGGGAGACTGAGTGGAGGGGTGAACAGACCCAGAAGTAAGTAATCATAG190070.485994327210952No Hit
AAAGCAGGCAGACTGGGACCGTCACCTCCCTCTCTCTGCACGCTCCACCT189660.4849459888400545No Hit
GAGTGGAGGGGTGAACAGACCCAGAAGTAAGTAATCATAGAGGGAGGCAA177180.4530355915990766No Hit
AGCGAAAGCAGGGGAATAATATACCGGAACCAAGGGAGACTGAGTGGAGG160400.41013042607795397No Hit
GAACCAAGGGAGACTGAGTGGAGGGGTGAACAGACCCAGAAGTAAGTAAT157860.4036358420241011No Hit
TAATATACCGGAACCAAGGGAGACTGAGTGGAGGGGTGAACAGACCCAGA152760.39059553545927833No Hit
ACCCAGAAGTAAGTAATCATAGAGGGAGGCAAAGGAGAGGAGAGGAAGCA150840.3856862435760509No Hit
AACCAAGGGAGACTGAGTGGAGGGGTGAACAGACCCAGAAGTAAGTAATC150310.3843310744624517No Hit
CTTGTGGGGAATGCTTCCTCTCCTCTCCTTTGCCTCCCTCTATGATTACT144040.3682991681562873No Hit
GGGGTGAACAGACCCAGAAGTAAGTAATCATAGAGGGAGGCAAAGGAGAG142430.3641825223583727No Hit
GACCCAGAAGTAAGTAATCATAGAGGGAGGCAAAGGAGAGGAGAGGAAGC137590.3518070157360703No Hit
CTATGATTACTTACTTCTGGGTCTGTTCACCCCTCCACTCAGTCTCCCTT134630.34423852408276145No Hit
GAAGTAAGTAATCATAGAGGGAGGCAAAGGAGAGGAGAGGAAGCATTCCC131530.3363120632296339No Hit
CCCTTGTGGGGAATGCTTCCTCTCCTCTCCTTTGCCTCCCTCTATGATTA130550.3338062788309033No Hit
CCCTCTCTCTGCACGCTCCACCTCTATTAATGAATCTGGGCTCCTACAGA123860.31670046492528287No Hit
CCTCTATGATTACTTACTTCTGGGTCTGTTCACCCCTCCACTCAGTCTCC115560.2954780052217479No Hit
CTTCTGGGTCTGTTCACCCCTCCACTCAGTCTCCCTTGGTTCCGGTATAT108690.2779119452020749No Hit
CCTCCCTCTCTCTGCACGCTCCACCTCTATTAATGAATCTGGGCTCCTAC108390.2771448683453206No Hit
GTAATCATAGAGGGAGGCAAAGGAGAGGAGAGGAAGCATTCCCCACAAGG106690.2727980994903797No Hit
GTGGAGGGGTGAACAGACCCAGAAGTAAGTAATCATAGAGGGAGGCAAAG105100.268732592149582No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA94430.24145022527768822No Hit
ATATACCGGAACCAAGGGAGACTGAGTGGAGGGGTGAACAGACCCAGAAG92480.23646422570878542No Hit
TCTCTGCACGCTCCACCTCTATTAATGAATCTGGGCTCCTACAGACCCTT90060.23027647239763424No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA88940.22741271879908492No Hit
CTCCTCTCCTTTGCCTCCCTCTATGATTACTTACTTCTGGGTCTGTTCAC88870.22723373419917559No Hit
CCTTTGCCTCCCTCTATGATTACTTACTTCTGGGTCTGTTCACCCCTCCA85970.21981865791721758No Hit
CCCCACAAGGGTCTGTAGGAGCCCAGATTCATTAATAGAGGTGGAGCGTG80360.2054743206959126No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT78970.20192019792628443No Hit
CTGCACGCTCCACCTCTATTAATGAATCTGGGCTCCTACAGACCCTTGTG78170.19987465964160636No Hit
GGAGAGGAGAGGAAGCATTCCCCACAAGGGTCTGTAGGAGCCCAGATTCA77290.1976245675284605No Hit
AGCGAAAGCAGGCAGACTGGGACCGTCACCTCCCTCTCTCTGCACGCTCC74060.18936570670407274No Hit
GGTCTGTAGGAGCCCAGATTCATTAATAGAGGTGGAGCGTGCAGAGAGAG72680.18583715316300306No Hit
GGCAGACTGGGACCGTCACCTCCCTCTCTCTGCACGCTCCACCTCTATTA72070.18427743022093604No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT69200.17693906162465345No Hit
CTCCTACAGACCCTTGTGGGGAATGCTTCCTCTCCTCTCCTTTGCCTCCC68710.17568616942528814No Hit
GAACAGACCCAGAAGTAAGTAATCATAGAGGGAGGCAAAGGAGAGGAGAG67960.17376847728340242No Hit
TACCGGAACCAAGGGAGACTGAGTGGAGGGGTGAACAGACCCAGAAGTAA67170.17174850822728283No Hit
GGGCTCCTACAGACCCTTGTGGGGAATGCTTCCTCTCCTCTCCTTTGCCT67000.17131383134178874No Hit
TAATCATAGAGGGAGGCAAAGGAGAGGAGAGGAAGCATTCCCCACAAGGG66900.17105813905620398No Hit
GGAATAATATACCGGAACCAAGGGAGACTGAGTGGAGGGGTGAACAGACC65610.16775970857216058No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT63550.16249244748911454No Hit
ACTTACTTCTGGGTCTGTTCACCCCTCCACTCAGTCTCCCTTGGTTCCGG61670.15768543252012107No Hit
AATCTGGGCTCCTACAGACCCTTGTGGGGAATGCTTCCTCTCCTCTCCTT61640.15760872483444566No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT61120.15627912494940488No Hit
GGTGAACAGACCCAGAAGTAAGTAATCATAGAGGGAGGCAAAGGAGAGGA59640.15249487912275045No Hit
CCTCTCCTTTGCCTCCCTCTATGATTACTTACTTCTGGGTCTGTTCACCC58790.15032149469528No Hit
GGGAGACTGAGTGGAGGGGTGAACAGACCCAGAAGTAAGTAATCATAGAG56440.14431272598403816No Hit
CATAGAGGGAGGCAAAGGAGAGGAGAGGAAGCATTCCCCACAAGGGTCTG56150.14357121835584236No Hit
AAGGAGAGGAGAGGAAGCATTCCCCACAAGGGTCTGTAGGAGCCCAGATT55070.14080974167152696No Hit
CCACCTCTATTAATGAATCTGGGCTCCTACAGACCCTTGTGGGGAATGCT55050.14075860321441No Hit
ATTAATGAATCTGGGCTCCTACAGACCCTTGTGGGGAATGCTTCCTCTCC54790.1400938032718896No Hit
CCCTCTATGATTACTTACTTCTGGGTCTGTTCACCCCTCCACTCAGTCTC54290.13881534184396582No Hit
CCTCTATTAATGAATCTGGGCTCCTACAGACCCTTGTGGGGAATGCTTCC53680.1372556189018988No Hit
TAATGAATCTGGGCTCCTACAGACCCTTGTGGGGAATGCTTCCTCTCCTC53440.13664195741649537No Hit
GGCTCCTACAGACCCTTGTGGGGAATGCTTCCTCTCCTCTCCTTTGCCTC52660.13464755758893424No Hit
CTTCCTCTCCTCTCCTTTGCCTCCCTCTATGATTACTTACTTCTGGGTCT51710.13221848087587904No Hit
GTAAGTAATCATAGAGGGAGGCAAAGGAGAGGAGAGGAAGCATTCCCCAC48840.12488011227959644No Hit
AGGCAAAGGAGAGGAGAGGAAGCATTCCCCACAAGGGTCTGTAGGAGCCC48570.12418974310851759No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC48130.12306469705194464No Hit
ATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGAT47340.12104472799582505No Hit
AGACTGAGTGGAGGGGTGAACAGACCCAGAAGTAAGTAATCATAGAGGGA47160.1205844818817725No Hit
CAGGCAGACTGGGACCGTCACCTCCCTCTCTCTGCACGCTCCACCTCTAT45500.11633998994106548No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA44700.11429445165638741No Hit
ACCTCTATTAATGAATCTGGGCTCCTACAGACCCTTGTGGGGAATGCTTC44690.11426888242782893No Hit
GTCACCTCCCTCTCTCTGCACGCTCCACCTCTATTAATGAATCTGGGCTC44240.11311826714269752No Hit
CTCCTTTGCCTCCCTCTATGATTACTTACTTCTGGGTCTGTTCACCCCTC43710.1117630980290983No Hit
GATTGAAAGCGACAGAGAATAGTTTCGAACAAATAACATTTATGCAAGCC42730.10925731363036766No Hit
CCACAAGGGTCTGTAGGAGCCCAGATTCATTAATAGAGGTGGAGCGTGCA40750.10419460637578941No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTC40500.10355537566182753No Hit
ATCTGGGCTCCTACAGACCCTTGTGGGGAATGCTTCCTCTCCTCTCCTTT40140.10263488343372239No Hit
AATATACCGGAACCAAGGGAGACTGAGTGGAGGGGTGAACAGACCCAGAA40080.10248146806237153No Hit
CATTAATAGAGGTGGAGCGTGCAGAGAGAGGGAGGTGACGGTCCCAGTCT40060.10243032960525458No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG39890.10199565271976048No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC39740.10161211429138337No Hit
AAGCAGGGGAATAATATACCGGAACCAAGGGAGACTGAGTGGAGGGGTGA39590.10122857586300621No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)