FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_H2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_H2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1004045
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATTCCTGGCTTTAAAGTTGAGAGAGTTGCCTGGGAATACTTTCGA178231.775119641051945No Hit
TCCTTGTTCCCTCTGCCACATCCTCCGTGGCTTGAGTTTCCTCTGTGAGG107971.0753502084069937No Hit
GGGTAGAGGAAGGGGCATGGAAGGAGCCACTCTGGGCTGGGCTGTCTCTG74180.7388115074523552No Hit
ACTCTGGGCTGGGCTGTCTCTGCTGATGACTGTTTTCTGGACGCCCTCGC67640.6736749846869413No Hit
GTGCTCAGCTGTGCATATGGATGGATGCTCAGGGCTGATATCTGAGCCGA66990.6672011712622442No Hit
GCTGAGCACTGGGGCATTTCTGTTACACCATTTTGCTGACCGGGCAGATC58990.5875234675736646No Hit
GGGCTGGGCTGTCTCTGCTGATGACTGTTTTCTGGACGCCCTCGCAGATC50910.507048986848199No Hit
CTTTAATGTGGCTGCAGTCACTGCCCTAGCATTAATTACGTTAACGTTTG49860.49659128823907295No Hit
GTCTAAGGCAGACGCCCAACGACTGAGCCTCCCAGGCGCCTCTCTTCCCT48920.48722915805566486No Hit
GTAGAGGAAGGGGCATGGAAGGAGCCACTCTGGGCTGGGCTGTCTCTGCT47510.4731859627805527No Hit
GTCTCTGCTGATGACTGTTTTCTGGACGCCCTCGCAGATCTGCCCGGTCA46370.46183189000493No Hit
AGCGAAAGCAGGGCAATTATATTCCTGGCTTTAAAGTTGAGAGAGTTGCC42450.42278981519752606No Hit
ATGCACAGCTGAGCACTGGGGCATTTCTGTTACACCATTTTGCTGACCGG41570.4140252677917822No Hit
CCTTTATGGAGCTTACAGTCGAGCGAGGGAGGCAAACGTTAACGTAATTA38670.3851421002046721No Hit
GCCACATCCTCCGTGGCTTGAGTTTCCTCTGTGAGGGTGAGGGAGCCAGT38600.384444920297397No Hit
TTATATTCCTGGCTTTAAAGTTGAGAGAGTTGCCTGGGAATACTTTCGAT36980.36831018530045967No Hit
GTCGAGAGGAGGGCTATAGCCTCGGCTCAGATATCAGCCCTGAGCATCCA36090.35944604076510517No Hit
CCATAAAGGTAGGAAGCGCCTTCTGTCTTTCCCTTCCTGGCTGTCTGCCC33240.33106085882604863No Hit
GGGCATGGAAGGAGCCACTCTGGGCTGGGCTGTCTCTGCTGATGACTGTT30830.307057950589864No Hit
AGCGAAAGCAGGCCATCCTTGTTCCCTCTGCCACATCCTCCGTGGCTTGA30740.3061615764233675No Hit
GGGCAATTATATTCCTGGCTTTAAAGTTGAGAGAGTTGCCTGGGAATACT30270.3014805113316634No Hit
CCCCAGGATCATGACCTAGGTCTAAGGCAGACGCCCAACGACTGAGCCTC29830.29709823762879156No Hit
GTGTAACAGAAATGCCCCAGTGCTCAGCTGTGCATATGGATGGATGCTCA29270.29152079837059097No Hit
ACTGTAAGCTCCATAAAGGTAGGAAGCGCCTTCTGTCTTTCCCTTCCTGG28070.279569142817304No Hit
GCCCTAGCATTAATTACGTTAACGTTTGCCTCCCTCGCTCGACTGTAAGC27810.27697961744742516No Hit
CTGTTACACCATTTTGCTGACCGGGCAGATCTGCGAGGGCGTCCAGAAAA27480.27369291217027125No Hit
TCCATATGCACAGCTGAGCACTGGGGCATTTCTGTTACACCATTTTGCTG27370.2725973437445533No Hit
GCTTACAGTCGAGCGAGGGAGGCAAACGTTAACGTAATTAATGCTAGGGC26920.26811547291207066No Hit
GCTGTGCATATGGATGGATGCTCAGGGCTGATATCTGAGCCGAGGCTATA26720.2661235303198562No Hit
GCCCTGGGCAGACAGCCAGGAAGGGAAAGACAGAAGGCGCTTCCTACCTT26560.2645299762460846No Hit
GGTCTAAGGCAGACGCCCAACGACTGAGCCTCCCAGGCGCCTCTCTTCCC26480.2637331992091988No Hit
CTGTGAGGGTGAGGGAGCCAGTCGAGAGGAGGGCTATAGCCTCGGCTCAG26240.2613428680985414No Hit
AAAGCAGGCCATCCTTGTTCCCTCTGCCACATCCTCCGTGGCTTGAGTTT26120.2601477025432127No Hit
ACCCAGCAGGGAGCCCCATGCAGGGCTCAATCCCAGGACCCCAGGATCAT26100.25994850828399124No Hit
GGCCATCCTTGTTCCCTCTGCCACATCCTCCGTGGCTTGAGTTTCCTCTG25750.2564626087476159No Hit
GCTATAGCCTCGGCTCAGATATCAGCCCTGAGCATCCATCCATATGCACA25340.2523791264335762No Hit
CATATGCACAGCTGAGCACTGGGGCATTTCTGTTACACCATTTTGCTGAC25200.250984766619026No Hit
TATCAGCCCTGAGCATCCATCCATATGCACAGCTGAGCACTGGGGCATTT24930.24829564411953647No Hit
TATTGAGGCTTTTTAAAGTGCCAAGGCCCTGGGCAGACAGCCAGGAAGGG24690.2459053130088791No Hit
GCTCAGATATCAGCCCTGAGCATCCATCCATATGCACAGCTGAGCACTGG24490.2439133704166646No Hit
GTGCATATGGATGGATGCTCAGGGCTGATATCTGAGCCGAGGCTATAGCC23870.23773834838079966No Hit
ACATTAAAGAGGGAAGAGAGGCGCCTGGGAGGCTCAGTCGTTGGGCGTCT23860.23763875125118894No Hit
CCCTCGCAGATCTGCCCGGTCAGCAAAATGGTGTAACAGAAATGCCCCAG23490.23395365745559213No Hit
CATTAAAGAGGGAAGAGAGGCGCCTGGGAGGCTCAGTCGTTGGGCGTCTG23100.23006936940077388No Hit
TGCTAGGGCAGTGACTGCAGCCACATTAAAGAGGGAAGAGAGGCGCCTGG23040.2294717866231095No Hit
GAAGGAGCCACTCTGGGCTGGGCTGTCTCTGCTGATGACTGTTTTCTGGA22870.2277786354197272No Hit
CTTTAAAAAGCCTCAATAAGCATCGAAAGTATTCCCAGGCAACTCTCTCA22800.22708145551245212No Hit
CCTTGTTCCCTCTGCCACATCCTCCGTGGCTTGAGTTTCCTCTGTGAGGG22750.2265834698643985No Hit
GGGCTATAGCCTCGGCTCAGATATCAGCCCTGAGCATCCATCCATATGCA22080.21991046218047994No Hit
CTTCCTACCTTTATGGAGCTTACAGTCGAGCGAGGGAGGCAAACGTTAAC21770.2168229511625475No Hit
GTGAGGGTGAGGGAGCCAGTCGAGAGGAGGGCTATAGCCTCGGCTCAGAT21500.21413382866305794No Hit
CTGCCACATCCTCCGTGGCTTGAGTTTCCTCTGTGAGGGTGAGGGAGCCA21440.21353624588539358No Hit
ACCTAGGTCTAAGGCAGACGCCCAACGACTGAGCCTCCCAGGCGCCTCTC21300.21214188607084344No Hit
TCGCAGATCTGCCCGGTCAGCAAAATGGTGTAACAGAAATGCCCCAGTGC21220.21134510903395762No Hit
AGCATTAATTACGTTAACGTTTGCCTCCCTCGCTCGACTGTAAGCTCCAT21190.21104631764512546No Hit
GCCCTGAGCATCCATCCATATGCACAGCTGAGCACTGGGGCATTTCTGTT21140.21054833199707185No Hit
GAGCCACTCTGGGCTGGGCTGTCTCTGCTGATGACTGTTTTCTGGACGCC21130.21044873486746113No Hit
GTTGAGAGAGTTGCCTGGGAATACTTTCGATGCTTATTGAGGCTTTTTAA20780.20696283533108575No Hit
GATATCAGCCCTGAGCATCCATCCATATGCACAGCTGAGCACTGGGGCAT20400.20317814440587822No Hit
GCCCCATGCAGGGCTCAATCCCAGGACCCCAGGATCATGACCTAGGTCTA20150.2006882161656101No Hit
ATTAATGCTAGGGCAGTGACTGCAGCCACATTAAAGAGGGAAGAGAGGCG20130.20048902190638865No Hit
GCCAAGGCCCTGGGCAGACAGCCAGGAAGGGAAAGACAGAAGGCGCTTCC19990.19909466209183851No Hit
GAGTTTCCTCTGTGAGGGTGAGGGAGCCAGTCGAGAGGAGGGCTATAGCC19970.19889546783261708No Hit
TATCTGAGCCGAGGCTATAGCCCTCCTCTCGACTGGCTCCCTCACCCTCA19950.19869627357339562No Hit
GTCACTGCCCTAGCATTAATTACGTTAACGTTTGCCTCCCTCGCTCGACT19810.19730191375884548No Hit
AGCAGGGGGAGTGGGAGGAGGAGAAGCAGGCTCCCCACCCAGCAGGGAGC19630.19550916542585245No Hit
TCCCAGGACCCCAGGATCATGACCTAGGTCTAAGGCAGACGCCCAACGAC19360.19282004292636287No Hit
CCTCTGTGAGGGTGAGGGAGCCAGTCGAGAGGAGGGCTATAGCCTCGGCT19210.191326085982202No Hit
GCCTTAGACCTAGGTCATGATCCTGGGGTCCTGGGATTGAGCCCTGCATG18770.1869438122793301No Hit
GATCTGCCCGGTCAGCAAAATGGTGTAACAGAAATGCCCCAGTGCTCAGC18720.18644582663127648No Hit
GGCATGGAAGGAGCCACTCTGGGCTGGGCTGTCTCTGCTGATGACTGTTT18650.18574864672400143No Hit
AAGCAGGAGGGTAGAGGAAGGGGCATGGAAGGAGCCACTCTGGGCTGGGC18510.18435428690945127No Hit
GCCCAGGGCCTTGGCACTTTAAAAAGCCTCAATAAGCATCGAAAGTATTC18310.18236234431723677No Hit
GTTTTCTGGACGCCCTCGCAGATCTGCCCGGTCAGCAAAATGGTGTAACA18230.18156556728035098No Hit
GGGCTGTCTCTGCTGATGACTGTTTTCTGGACGCCCTCGCAGATCTGCCC17520.17449417107798953No Hit
ATGCAGGGCTCAATCCCAGGACCCCAGGATCATGACCTAGGTCTAAGGCA17310.17240263135616432No Hit
GAATACTTTCGATGCTTATTGAGGCTTTTTAAAGTGCCAAGGCCCTGGGC17250.17180504857849999No Hit
GCATTAATTACGTTAACGTTTGCCTCCCTCGCTCGACTGTAAGCTCCATA17130.17060988302317126No Hit
TTTTTAAAGTGCCAAGGCCCTGGGCAGACAGCCAGGAAGGGAAAGACAGA17030.16961391172706403No Hit
GAGGAAGGGGCATGGAAGGAGCCACTCTGGGCTGGGCTGTCTCTGCTGAT16750.16682519209796373No Hit
CAATTATATTCCTGGCTTTAAAGTTGAGAGAGTTGCCTGGGAATACTTTC16740.166725594968353No Hit
CCCAACGACTGAGCCTCCCAGGCGCCTCTCTTCCCTCTTTAATGTGGCTG16720.16652640070913158No Hit
GGGAAAGACAGAAGGCGCTTCCTACCTTTATGGAGCTTACAGTCGAGCGA16640.16572962367224575No Hit
GGTAGGAAGCGCCTTCTGTCTTTCCCTTCCTGGCTGTCTGCCCAGGGCCT16630.16563002654263503No Hit
AGCGAAAGCAGGAGGGTAGAGGAAGGGGCATGGAAGGAGCCACTCTGGGC16630.16563002654263503No Hit
TCCTACCTTTATGGAGCTTACAGTCGAGCGAGGGAGGCAAACGTTAACGT16610.1654308322834136No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA16420.16353848682080982No Hit
AATTAATGCTAGGGCAGTGACTGCAGCCACATTAAAGAGGGAAGAGAGGC16300.16234332126548115No Hit
GGCTATAGCCTCGGCTCAGATATCAGCCCTGAGCATCCATCCATATGCAC16190.16124775283976317No Hit
CTTGTTCCCTCTGCCACATCCTCCGTGGCTTGAGTTTCCTCTGTGAGGGT16120.1605505729324881No Hit
TCCCAGGCGCCTCTCTTCCCTCTTTAATGTGGCTGCAGTCACTGCCCTAG16110.16045097580287737No Hit
ACGTTAACGTTTGCCTCCCTCGCTCGACTGTAAGCTCCATAAAGGTAGGA15910.15845903321066288No Hit
CTTTCGATGCTTATTGAGGCTTTTTAAAGTGCCAAGGCCCTGGGCAGACA15900.15835943608105216No Hit
TTTCTGGACGCCCTCGCAGATCTGCCCGGTCAGCAAAATGGTGTAACAGA15710.15646709061844838No Hit
GGCAGTGACTGCAGCCACATTAAAGAGGGAAGAGAGGCGCCTGGGAGGCT15700.15636749348883766No Hit
ATCATGACCTAGGTCTAAGGCAGACGCCCAACGACTGAGCCTCCCAGGCG15700.15636749348883766No Hit
CTTTAAAGTTGAGAGAGTTGCCTGGGAATACTTTCGATGCTTATTGAGGC15660.15596910497039476No Hit
CGTTAACGTAATTAATGCTAGGGCAGTGACTGCAGCCACATTAAAGAGGG15630.15567031358156258No Hit
AGCGAAAGCAGGGGGAGTGGGAGGAGGAGAAGCAGGCTCCCCACCCAGCA15470.15407675950779098No Hit
TTAAAAAGCCTCAATAAGCATCGAAAGTATTCCCAGGCAACTCTCTCAAC15460.15397716237818027No Hit
TCTTTAATGTGGCTGCAGTCACTGCCCTAGCATTAATTACGTTAACGTTT15290.15228401117479795No Hit
GATCATGACCTAGGTCTAAGGCAGACGCCCAACGACTGAGCCTCCCAGGC15150.15088965136024782No Hit
CCCATGCAGGGCTCAATCCCAGGACCCCAGGATCATGACCTAGGTCTAAG15100.15039166571219417No Hit
GGTAGAGGAAGGGGCATGGAAGGAGCCACTCTGGGCTGGGCTGTCTCTGC15020.14959488867530837No Hit
GCTGATGACTGTTTTCTGGACGCCCTCGCAGATCTGCCCGGTCAGCAAAA15020.14959488867530837No Hit
GTGCCAAGGCCCTGGGCAGACAGCCAGGAAGGGAAAGACAGAAGGCGCTT14850.14790173747192606No Hit
GCTCAATCCCAGGACCCCAGGATCATGACCTAGGTCTAAGGCAGACGCCC14650.14590979487971156No Hit
GCAAAATGGTGTAACAGAAATGCCCCAGTGCTCAGCTGTGCATATGGATG14520.14461503219477215No Hit
GCTCAGGGCTGATATCTGAGCCGAGGCTATAGCCCTCCTCTCGACTGGCT13860.13804162164046432No Hit
GAGGAGAAGCAGGCTCCCCACCCAGCAGGGAGCCCCATGCAGGGCTCAAT13720.13664726182591416No Hit
GCCAGGAAGGGAAAGACAGAAGGCGCTTCCTACCTTTATGGAGCTTACAG13640.13585048478902836No Hit
GCTTTAAAGTTGAGAGAGTTGCCTGGGAATACTTTCGATGCTTATTGAGG13640.13585048478902836No Hit
TTATTGAGGCTTTTTAAAGTGCCAAGGCCCTGGGCAGACAGCCAGGAAGG13370.13316136228953881No Hit
GGGTGAGGGAGCCAGTCGAGAGGAGGGCTATAGCCTCGGCTCAGATATCA13360.1330617651599281No Hit
ATGCTAGGGCAGTGACTGCAGCCACATTAAAGAGGGAAGAGAGGCGCCTG13340.13286257090070663No Hit
GACAGAAGGCGCTTCCTACCTTTATGGAGCTTACAGTCGAGCGAGGGAGG13320.1326633766414852No Hit
GGGCTGATATCTGAGCCGAGGCTATAGCCCTCCTCTCGACTGGCTCCCTC13170.13116941969732432No Hit
CTGCTGATGACTGTTTTCTGGACGCCCTCGCAGATCTGCCCGGTCAGCAA13080.1302730455308278No Hit
GCATATGGATGGATGCTCAGGGCTGATATCTGAGCCGAGGCTATAGCCCT13080.1302730455308278No Hit
GGCTATAGCCCTCCTCTCGACTGGCTCCCTCACCCTCACAGAGGAAACTC13000.129476268493942No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT12760.12708593738328464No Hit
TCCCTCTTTAATGTGGCTGCAGTCACTGCCCTAGCATTAATTACGTTAAC12490.12439681488379505No Hit
TCTCTTCCCTCTTTAATGTGGCTGCAGTCACTGCCCTAGCATTAATTACG12410.12360003784690926No Hit
AAAGCAGGGGGAGTGGGAGGAGGAGAAGCAGGCTCCCCACCCAGCAGGGA12370.12320164932846636No Hit
CCATTTTGCTGACCGGGCAGATCTGCGAGGGCGTCCAGAAAACAGTCATC12330.12280326081002346No Hit
GGGCAGACAGCCAGGAAGGGAAAGACAGAAGGCGCTTCCTACCTTTATGG12320.12270366368041273No Hit
CTCTGCCACATCCTCCGTGGCTTGAGTTTCCTCTGTGAGGGTGAGGGAGC12060.12011413831053389No Hit
CCCCAGTGCTCAGCTGTGCATATGGATGGATGCTCAGGGCTGATATCTGA11880.11832138997754085No Hit
CAGGAGGGTAGAGGAAGGGGCATGGAAGGAGCCACTCTGGGCTGGGCTGT11820.11772380719987649No Hit
CAGCAAAATGGTGTAACAGAAATGCCCCAGTGCTCAGCTGTGCATATGGA11660.1161302531261049No Hit
GTCTTTCCCTTCCTGGCTGTCTGCCCAGGGCCTTGGCACTTTAAAAAGCC11540.11493508757077621No Hit
GGAAGGGGCATGGAAGGAGCCACTCTGGGCTGGGCTGTCTCTGCTGATGA11480.11433750479311186No Hit
TCCTCTGTGAGGGTGAGGGAGCCAGTCGAGAGGAGGGCTATAGCCTCGGC11360.11314233923778318No Hit
CCTCTCTTCCCTCTTTAATGTGGCTGCAGTCACTGCCCTAGCATTAATTA11330.11284354784895098No Hit
CTCCGTGGCTTGAGTTTCCTCTGTGAGGGTGAGGGAGCCAGTCGAGAGGA11140.11095120238634722No Hit
GGAGGGTAGAGGAAGGGGCATGGAAGGAGCCACTCTGGGCTGGGCTGTCT11000.10955684257179707No Hit
GAAATGCCCCAGTGCTCAGCTGTGCATATGGATGGATGCTCAGGGCTGAT10980.10935764831257563No Hit
GCCTGGGAATACTTTCGATGCTTATTGAGGCTTTTTAAAGTGCCAAGGCC10960.10915845405335418No Hit
GAGTGGGAGGAGGAGAAGCAGGCTCCCCACCCAGCAGGGAGCCCCATGCA10560.10517456886892519No Hit
AAGCAGGCCATCCTTGTTCCCTCTGCCACATCCTCCGTGGCTTGAGTTTC10550.10507497173931447No Hit
CCTCTCGACTGGCTCCCTCACCCTCACAGAGGAAACTCAAGCCACGGAGG10540.10497537460970376No Hit
ATCCATATGCACAGCTGAGCACTGGGGCATTTCTGTTACACCATTTTGCT10510.10467658322087159No Hit
CCCTAGCATTAATTACGTTAACGTTTGCCTCCCTCGCTCGACTGTAAGCT10440.10397940331359651No Hit
GCCTCAATAAGCATCGAAAGTATTCCCAGGCAACTCTCTCAACTTTAAAG10430.10387980618398578No Hit
TTATGGAGCTTACAGTCGAGCGAGGGAGGCAAACGTTAACGTAATTAATG10280.1023858492398249No Hit
ATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGAT10150.10109108655488548No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)