FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_H10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_H10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6600702
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAA775721.1752083339014547No Hit
GGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCA748671.1342278442505054No Hit
CAGTATGGCTGACTAAAACCACAGAAGTCTTACCTTTTCATCTTTGATTT715941.0846422092680446No Hit
ATGTAGCAGAGGACAAATAAGGGAAAATCAAAGATGAAAAGGTAAGACTT594710.9009799260745296No Hit
ATCTAGATATTAATCATTATTTCTTCCATTTGGTCCTCAGTTTGTCGTCT469630.7114849299362401No Hit
TAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAG418670.6342810204126773No Hit
CCCCAAGATTAATCCATTTTAGGCATCCTAGTTTGAGAAAAACATGCACA404610.6129802557364352No Hit
AGTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCAAAAGGACTCAA396380.6005118849479949No Hit
TTCTCTCTCTCTGTCAAATAAAATCTTGAAATAATGAATCTTTTGTTCTT381020.5772416327839069No Hit
GTATGGCTGACTAAAACCACAGAAGTCTTACCTTTTCATCTTTGATTTTC363140.5501536048741482No Hit
GCCATACTGTGAGGAAAGAAGAAAGAACAAAAGATTCATTATTTCAAGAT354060.5363974922667316No Hit
CTGCTACATCTTGCCAGTCATTGAGTCCTTTTGATTCAACCTCTTAAGTG346810.5254138120460521No Hit
CTTTCTCTCTCTCTGTCAAATAAAATCTTGAAATAATGAATCTTTTGTTC329560.499280228072711No Hit
TATTTGTCCTCTGCTACATCTTGCCAGTCATTGAGTCCTTTTGATTCAAC323760.4904932838961674No Hit
GTTTTATTTTTATGGTGCCTTAGTACTTTTGAATTGGTTTTTGTGAATGC320760.485948312770369No Hit
ATTTTAGGCATCCTAGTTTGAGAAAAACATGCACAACTGAATTTGAAGAC320440.4854635158502838No Hit
GTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAG310880.4709802078627394No Hit
ATCTTGGGGTGCCTAGGTGGCTTGGTCGGTTAGTGTCTGCCTTTGGCTCA298810.45269427403327706No Hit
TATCTAGATAAAGCATTCACAAAAACCAATTCAAAAGTACTAAGGCACCA295660.4479220543511887No Hit
ATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAA284780.43143895906829305No Hit
GCTTTATCTAGATATTAATCATTATTTCTTCCATTTGGTCCTCAGTTTGT280740.4253183979522178No Hit
AGATAAAGCATTCACAAAAACCAATTCAAAAGTACTAAGGCACCATAAAA276970.41960688423746445No Hit
GATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTA253990.3847924054138484No Hit
GACTAAAACCACAGAAGTCTTACCTTTTCATCTTTGATTTTCCCTTATTT249170.3774901518050656No Hit
CTCTTTCTCTCTCTCTGTCAAATAAAATCTTGAAATAATGAATCTTTTGT243460.36883955676229585No Hit
CTTAAGAGGTTGAATCAAAAGGACTCAATGACTGGCAAGATGTAGCAGAG240130.3637946388126596No Hit
AGTCTTACCTTTTCATCTTTGATTTTCCCTTATTTGTCCTCTGCTACATC236380.35811342490541154No Hit
ATATTAATCATTATTTCTTCCATTTGGTCCTCAGTTTGTCGTCTTCAAAT235990.3575225786590578No Hit
GTCAAATAAAATCTTGAAATAATGAATCTTTTGTTCTTTCTTCTTTCCTC235700.35708323145023063No Hit
CAGTAACTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAA234960.35596213857253367No Hit
GGACAAATAAGGGAAAATCAAAGATGAAAAGGTAAGACTTCTGTGGTTTT232440.35214436282686296No Hit
CTTTTGTTCTTTCTTCTTTCCTCACAGTATGGCTGACTAAAACCACAGAA207100.3137545067176188No Hit
GTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAGGTT197330.2989530507512686No Hit
GTACTAAGGCACCATAAAAATAAAACAGATAAAATTGGTTTTAAAAATAG182530.2765311931973296No Hit
ATTATATTCCTGGCTTTAAAGTTGAGAGAGTTGCCTGGGAATACTTTCGA179340.2716983739002306No Hit
TCATTATTTCTTCCATTTGGTCCTCAGTTTGTCGTCTTCAAATTCAGTTG179230.27153172495895134No Hit
GCCTTAGTACTTTTGAATTGGTTTTTGTGAATGCTTTATCTAGATATTAA178160.26991068525741657No Hit
GCCTAAAATGGATTAATCTTGGGGTGCCTAGGTGGCTTGGTCGGTTAGTG174770.26477486788526433No Hit
AGCAGGGAGAGAGCAGCAGGCAGCAGGATAAGTAGGCTTCCCACTGAGCA173200.2623963329960965No Hit
CTCTCTGTCAAATAAAATCTTGAAATAATGAATCTTTTGTTCTTTCTTCT172670.26159338809720545No Hit
GGTAAGACTTCTGTGGTTTTAGTCAGCCATACTGTGAGGAAAGAAGAAAG168510.2552910281360983No Hit
AGGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGA164350.2489886681749911No Hit
CTCTCTCTCTGTCAAATAAAATCTTGAAATAATGAATCTTTTGTTCTTTC164050.24853417106241124No Hit
AGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTT162040.24548904040812627No Hit
GCACTTAAGAGGTTGAATCAAAAGGACTCAATGACTGGCAAGATGTAGCA159790.24208031206377745No Hit
TCTTTCTTCTTTCCTCACAGTATGGCTGACTAAAACCACAGAAGTCTTAC156870.23765654016800034No Hit
ATTTTTATGGTGCCTTAGTACTTTTGAATTGGTTTTTGTGAATGCTTTAT153260.2321874249132895No Hit
GATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCAA152680.23130873049563516No Hit
GCCTGGGTGTGTCCACTGGCTAAGTGTCTGCCTTCAGCTCAGTTCCTGAC152060.23036943646297017No Hit
GATTAATATCTAGATAAAGCATTCACAAAAACCAATTCAAAAGTACTAAG152000.2302785370404542No Hit
GTCATTGAGTCCTTTTGATTCAACCTCTTAAGTGCTTTTTGCATTCTATT151040.22882414628019868No Hit
GATTAATCCATTTTAGGCATCCTAGTTTGAGAAAAACATGCACAACTGAA144820.21940090614604324No Hit
CCTTAGTACTTTTGAATTGGTTTTTGTGAATGCTTTATCTAGATATTAAT142070.21523468261406137No Hit
CTTAAGTGCTTTTTGCATTCTATTCACTTTCTCTAATCCTACTACCACTA141720.21470443598271818No Hit
GTGGAGAGCCTCCTTCTCCCTCTTCTGTTTCCCCTGCTTGTGCGCTCTTT138990.21056851225824164No Hit
GAGAGAAAGAGCGCACAAGCAGGGGAAACAGAAGAGGGAGAAGGAGGCTC136220.20637198891875438No Hit
TCCTGGGATTGAGCCCCACATTGAGCTCCCTGCTCAGTGGAGAGCCTCCT134410.20362985633952269No Hit
GTCTTACCTTTTCATCTTTGATTTTCCCTTATTTGTCCTCTGCTACATCT134140.2032208089382008No Hit
GAGGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTG133090.20163006904417138No Hit
TTTTTATGGTGCCTTAGTACTTTTGAATTGGTTTTTGTGAATGCTTTATC133070.20159976923666603No Hit
CTTTTGATTCAACCTCTTAAGTGCTTTTTGCATTCTATTCACTTTCTCTA130450.19763049445346872No Hit
TAACAGGGATGCCTGGGTGTGTCCACTGGCTAAGTGTCTGCCTTCAGCTC129960.19688814916958833No Hit
AATCAAAAGGACTCAATGACTGGCAAGATGTAGCAGAGGACAAATAAGGG129840.19670635032455638No Hit
CCTCACAGTATGGCTGACTAAAACCACAGAAGTCTTACCTTTTCATCTTT129510.19620640350071855No Hit
ATCCCAGGAAGGACCCTGGGATCATGACCTGAGCCAAAGGCAGACACTAA127610.19332792178771288No Hit
GGATAAGTAGGCTTCCCACTGAGCAGGGAGCTCCATGCAGTGCTCAATCC127470.1931158231351756No Hit
CAGCCATACTGTGAGGAAAGAAGAAAGAACAAAAGATTCATTATTTCAAG127350.1929340242901437No Hit
GATGTAGCAGAGGACAAATAAGGGAAAATCAAAGATGAAAAGGTAAGACT127020.19243407746630586No Hit
GCTCAGTGGAGAGCCTCCTTCTCCCTCTTCTGTTTCCCCTGCTTGTGCGC126340.19140388401112488No Hit
CTACTGAAGAAACAACAGGGGAGAGGTAACAGTAACTATAATAAGATAGT126010.19090393718728704No Hit
ATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCA125660.1903736905559439No Hit
GCTAATAACAGGGATGCCTGGGTGTGTCCACTGGCTAAGTGTCTGCCTTC125140.1895858955608055No Hit
GTTTTAGTCAGCCATACTGTGAGGAAAGAAGAAAGAACAAAAGATTCATT123080.18646501538775723No Hit
ATCTTGAAATAATGAATCTTTTGTTCTTTCTTCTTTCCTCACAGTATGGC121220.1836471332897622No Hit
ATCATGACCTGAGCCAAAGGCAGACACTAACCGACCAAGCCACCTAGGCA120840.18307143694716108No Hit
GAGAGAGAAAGAGCGCACAAGCAGGGGAAACAGAAGAGGGAGAAGGAGGC120050.18187459455070082No Hit
CACCCAGGCATCCCTGTTATTAGCTATTTTTAAAACCAATTTTATCTGTT119840.18155644657189493No Hit
AGATGTAGCAGAGGACAAATAAGGGAAAATCAAAGATGAAAAGGTAAGAC117710.17832951707257805No Hit
CAACAGGGGAGAGGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATT116120.17592068237590489No Hit
GCTACATCTTGCCAGTCATTGAGTCCTTTTGATTCAACCTCTTAAGTGCT115160.17446629161564936No Hit
GTCCTCAGTTTGTCGTCTTCAAATTCAGTTGTGCATGTTTTTCTCAAACT114940.17413299373309082No Hit
CACTTAGCCAGTGGACACACCCAGGCATCCCTGTTATTAGCTATTTTTAA113670.1722089559565028No Hit
CCACTGAGCAGGGAGCTCAATGTGGGGCTCAATCCCAGGAAGGACCCTGG112470.17039096750618343No Hit
TCACAGTATGGCTGACTAAAACCACAGAAGTCTTACCTTTTCATCTTTGA109670.16614899445543824No Hit
ATCCTAGTTTGAGAAAAACATGCACAACTGAATTTGAAGACGACAAACTG109650.16611869464793289No Hit
GGTCATGATCCCAGGGTCCTTCCTGGGATTGAGCCCCACATTGAGCTCCC109580.16601264532166427No Hit
ATCCCAGGACGCAGGGGTCAGGAACTGAGCTGAAGGCAGACACTTAGCCA108540.16443705533138747No Hit
CCACAGAAGTCTTACCTTTTCATCTTTGATTTTCCCTTATTTGTCCTCTG104130.1577559477764638No Hit
GAGAAAGAGCGCACAAGCAGGGGAAACAGAAGAGGGAGAAGGAGGCTCTC104020.1575892988351845No Hit
GAGCAGGGAGCTCCATGCAGTGCTCAATCCCAGGACGCAGGGGTCAGGAA103480.1567712040325408No Hit
ACATTGAGCTCCCTGCTCAGTGGAGAGCCTCCTTCTCCCTCTTCTGTTTC102580.15540771269480125No Hit
GGCTAAGTGTCTGCCTTCAGCTCAGTTCCTGACCCCTGCGTCCTGGGATT102450.15521076394601666No Hit
CTTCTTTCCTCACAGTATGGCTGACTAAAACCACAGAAGTCTTACCTTTT102170.15478656664094215No Hit
AATGAATCTTTTGTTCTTTCTTCTTTCCTCACAGTATGGCTGACTAAAAC101760.15416542058708302No Hit
CCCTTATTTGTCCTCTGCTACATCTTGCCAGTCATTGAGTCCTTTTGATT101510.15378667299326648No Hit
GTTAGTGTCTGCCTTTGGCTCAGGTCATGATCCCAGGGTCCTTCCTGGGA100170.15175658589040986No Hit
CCTCTTAAGTGCTTTTTGCATTCTATTCACTTTCTCTAATCCTACTACCA99840.15125663906657202No Hit
ACAGAAGTCTTACCTTTTCATCTTTGATTTTCCCTTATTTGTCCTCTGCT97820.14819635850853438No Hit
GGGGAGAGGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATTAGAGA97330.14745401322465398No Hit
CTCAATGACTGGCAAGATGTAGCAGAGGACAAATAAGGGAAAATCAAAGA96810.1466662182295156No Hit
TTATTTGACAGAGAGAGAGAAAGAGCGCACAAGCAGGGGAAACAGAAGAG96780.1466207685182576No Hit
GGTCAGGAACTGAGCTGAAGGCAGACACTTAGCCAGTGGACACACCCAGG96270.14584812342687187No Hit
GAGCAGGGAGCTCAATGTGGGGCTCAATCCCAGGAAGGACCCTGGGATCA96040.14549967564056066No Hit
CTATTCACTTTCTCTAATCCTACTACCACTATCTTATTATAGTTACTGTT94850.14369683709399395No Hit
ACTGAAGAAACAACAGGGGAGAGGTAACAGTAACTATAATAAGATAGTGG94620.14334838930768273No Hit
CTGTCAAATAAAATCTTGAAATAATGAATCTTTTGTTCTTTCTTCTTTCC93850.1421818467187278No Hit
TTATTTCTTCCATTTGGTCCTCAGTTTGTCGTCTTCAAATTCAGTTGTGC93590.14178794922115862No Hit
ATAATGAATCTTTTGTTCTTTCTTCTTTCCTCACAGTATGGCTGACTAAA91350.13839437078056244No Hit
CAGTTTGTCGTCTTCAAATTCAGTTGTGCATGTTTTTCTCAAACTAGGAT90550.1371823784803495No Hit
GGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAA89750.1359703861801366No Hit
AGATATTAATCATTATTTCTTCCATTTGGTCCTCAGTTTGTCGTCTTCAA89630.13578858733510465No Hit
CATTGAGTCCTTTTGATTCAACCTCTTAAGTGCTTTTTGCATTCTATTCA88500.13407664821105392No Hit
GCCTAGGTGGCTTGGTCGGTTAGTGTCTGCCTTTGGCTCAGGTCATGATC88040.1333797526384315No Hit
AGAAACAACAGGGGAGAGGTAACAGTAACTATAATAAGATAGTGGTAGTA86560.1311375668830376No Hit
GTTATTAGCTATTTTTAAAACCAATTTTATCTGTTTTATTTTTATGGTGC86200.13059217034794177No Hit
CTTCTGTTTCCCCTGCTTGTGCGCTCTTTCTCTCTCTCTGTCAAATAAAA86130.13048612102167315No Hit
TCCCTGTTATTAGCTATTTTTAAAACCAATTTTATCTGTTTTATTTTTAT84590.12815303584376328No Hit
ACTGTGAGGAAAGAAGAAAGAACAAAAGATTCATTATTTCAAGATTTTAT84520.12804698651749463No Hit
AGCGAAAGCAGGCTACTGAAGAAACAACAGGGGAGAGGTAACAGTAACTA84420.12789548747996804No Hit
ACAGGGGAGAGGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATTAG84050.12733494104111956No Hit
CAAATAAGGGAAAATCAAAGATGAAAAGGTAAGACTTCTGTGGTTTTAGT83900.12710769248482964No Hit
GGCAAGATGTAGCAGAGGACAAATAAGGGAAAATCAAAGATGAAAAGGTA83870.12706224277357164No Hit
TAATGAATCTTTTGTTCTTTCTTCTTTCCTCACAGTATGGCTGACTAAAA83670.12675924469851843No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC83450.12642594681595987No Hit
GAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCAAAAGGA82970.12569875143583215No Hit
GCTCCATGCAGTGCTCAATCCCAGGACGCAGGGGTCAGGAACTGAGCTGA81390.12330506664291162No Hit
GGATTAATCTTGGGGTGCCTAGGTGGCTTGGTCGGTTAGTGTCTGCCTTT81160.1229566188566004No Hit
GAACAAAAGATTCATTATTTCAAGATTTTATTTGACAGAGAGAGAGAAAG80580.12207792443894605No Hit
CCTTTTCATCTTTGATTTTCCCTTATTTGTCCTCTGCTACATCTTGCCAG77990.11815409936700673No Hit
GGAGAGAGCAGCAGGCAGCAGGATAAGTAGGCTTCCCACTGAGCAGGGAG77810.11788140109945881No Hit
AAATAAAACAGATAAAATTGGTTTTAAAAATAGCTAATAACAGGGATGCC77320.1171390558155784No Hit
GGTTTTTGTGAATGCTTTATCTAGATATTAATCATTATTTCTTCCATTTG76580.11601796293788146No Hit
GGCTACTGAAGAAACAACAGGGGAGAGGTAACAGTAACTATAATAAGATA76100.11529076755775372No Hit
GCTCAATGTGGGGCTCAATCCCAGGAAGGACCCTGGGATCATGACCTGAG75670.11463932169638927No Hit
AAAGCAGGCTACTGAAGAAACAACAGGGGAGAGGTAACAGTAACTATAAT75350.11415452477630408No Hit
GTGTCTGCCTTTGGCTCAGGTCATGATCCCAGGGTCCTTCCTGGGATTGA73960.11204868815468416No Hit
AACTAGGATGCCTAAAATGGATTAATCTTGGGGTGCCTAGGTGGCTTGGT73720.11168509046462027No Hit
CACCTAGGCACCCCAAGATTAATCCATTTTAGGCATCCTAGTTTGAGAAA73580.111472991812083No Hit
AACATGCACAACTGAATTTGAAGACGACAAACTGAGGACCAAATGGAAGA72980.11056399758692334No Hit
AACTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCA72960.110533697779418No Hit
GACACTAACCGACCAAGCCACCTAGGCACCCCAAGATTAATCCATTTTAG72190.10936715519046308No Hit
ATCCCAGGGTCCTTCCTGGGATTGAGCCCCACATTGAGCTCCCTGCTCAG71900.1089278079816359No Hit
TCTTTCCTCACAGTATGGCTGACTAAAACCACAGAAGTCTTACCTTTTCA71480.10829151202402412No Hit
CCCTGGGATCATGACCTGAGCCAAAGGCAGACACTAACCGACCAAGCCAC71420.10820061260150814No Hit
GGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAGGT71410.10818546269775549No Hit
GTGAGGAAAGAAGAAAGAACAAAAGATTCATTATTTCAAGATTTTATTTG71360.10810971317899216No Hit
GGCAGACACTAACCGACCAAGCCACCTAGGCACCCCAAGATTAATCCATT71130.10776126539268095No Hit
CCTAGGCACCCCAAGATTAATCCATTTTAGGCATCCTAGTTTGAGAAAAA70790.10724616866509049No Hit
AAAGTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCAAAAGGACTC70640.10701892010880054No Hit
GTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCAAAAGGACTCAAT70590.10694317059003725No Hit
GATGAAAAGGTAAGACTTCTGTGGTTTTAGTCAGCCATACTGTGAGGAAA70500.10680682145626327No Hit
GGGGAAACAGAAGAGGGAGAAGGAGGCTCTCCACTGAGCAGGGAGCTCAA70310.10651897328496271No Hit
GAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCAAAAGGACTCAATGA69980.10601902646112489No Hit
GAAGAAACAACAGGGGAGAGGTAACAGTAACTATAATAAGATAGTGGTAG69410.1051554819472232No Hit
GTTCCTGACCCCTGCGTCCTGGGATTGAGCACTGCATGGAGCTCCCTGCT69280.10495853319843859No Hit
GCTCTCCACTGAGCAGGGAGCTCAATGTGGGGCTCAATCCCAGGAAGGAC68950.10445858637460076No Hit
ATGTGGGGCTCAATCCCAGGAAGGACCCTGGGATCATGACCTGAGCCAAA68790.10421618791455818No Hit
CTCTAATCCTACTACCACTATCTTATTATAGTTACTGTTACCTCTCCCCT68360.10356474205319374No Hit
GAAGAAAGAACAAAAGATTCATTATTTCAAGATTTTATTTGACAGAGAGA67950.10294359599933461No Hit
CCATAAAAATAAAACAGATAAAATTGGTTTTAAAAATAGCTAATAACAGG67430.10215580100419622No Hit
GCAAAAAGCACTTAAGAGGTTGAATCAAAAGGACTCAATGACTGGCAAGA67350.10203460177417494No Hit
GTCCTTTTGATTCAACCTCTTAAGTGCTTTTTGCATTCTATTCACTTTCT67200.101807353217885No Hit
GCAGAGGACAAATAAGGGAAAATCAAAGATGAAAAGGTAAGACTTCTGTG66960.10144375552782114No Hit
AATAAGGGAAAATCAAAGATGAAAAGGTAAGACTTCTGTGGTTTTAGTCA66690.10103470812649927No Hit
GAAATAATGAATCTTTTGTTCTTTCTTCTTTCCTCACAGTATGGCTGACT66330.10048931159140347No Hit
ATACTGTGAGGAAAGAAGAAAGAACAAAAGATTCATTATTTCAAGATTTT66150.10021661332385555No Hit
CAAAAGGACTCAATGACTGGCAAGATGTAGCAGAGGACAAATAAGGGAAA66140.1002014634201029No Hit

[FAIL]Adapter Content

Adapter graph