FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_G7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_G7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4016285
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTAAAGTGACTTTCCTGACGGGTTAGCTCTGAAGCCCTCTGTGGCTA362140.9016790392116096No Hit
CCCCCACCCCAGGCCATGTTAGGTTTAATCAGGACCTGGTTTTCAGTGAG278750.6940493515773906No Hit
CCCCAGGCCATGTTAGGTTTAATCAGGACCTGGTTTTCAGTGAGATGAAG268770.6692005173935615No Hit
ATGTTAGGTTTAATCAGGACCTGGTTTTCAGTGAGATGAAGGTTCAGGGA266090.6625276841658398No Hit
GCTCTGAAGCCCTCTGTGGCTATCAATGCCCAGCAGGAGTCGGTCCTCAA238840.5946789134735209No Hit
GGTTTAATCAGGACCTGGTTTTCAGTGAGATGAAGGTTCAGGGAAGTGAT219920.5475707027763219No Hit
AAGCAGGACTCTAAAGTGACTTTCCTGACGGGTTAGCTCTGAAGCCCTCT219620.5468237438329202No Hit
GGTTAAGTGTCTGCCTTCAGTTCAGGTCTTGATCTCCGGGTCCTGGGATG214730.5346483130554729No Hit
GTAGGTTAAGTGTCTGCCTTCAGTTCAGGTCTTGATCTCCGGGTCCTGGG210500.5241161919535092No Hit
GTTCAGGGAAGTGATTGACAAAAATGCTGTTTCTCCAGAGTGTTTGCTAC202320.5037491114300903No Hit
TCTCCAGAGTGTTTGCTACTTGAGGACCGACTCCTGCTGGGCATTGATAG186360.4640108956411211No Hit
GGTTAGCTCTGAAGCCCTCTGTGGCTATCAATGCCCAGCAGGAGTCGGTC176280.43891307514282474No Hit
GAGTAGCAGAGGGACAGGAAGCAGCAGACTCCCAGCTGAGCAGGGAGCCT175110.4359999352635582No Hit
GTCCTCAAGTAGCAAACACTCTGGAGAAACAGCATTTTTGTCAATCACTT170160.4236751126974306No Hit
GGTTTTCAGTGAGATGAAGGTTCAGGGAAGTGATTGACAAAAATGCTGTT159280.39658540168339645No Hit
GATGAAGGTTCAGGGAAGTGATTGACAAAAATGCTGTTTCTCCAGAGTGT159270.39656050305194973No Hit
AGCGAAAGCAGGACTCTAAAGTGACTTTCCTGACGGGTTAGCTCTGAAGC156980.3908587164506503No Hit
CTAAAGTGACTTTCCTGACGGGTTAGCTCTGAAGCCCTCTGTGGCTATCA156580.3898627711927814No Hit
CTCTGGAGAAACAGCATTTTTGTCAATCACTTCCCTGAACCTTCATCTCA155670.38759699573112966No Hit
AATCAGGACCTGGTTTTCAGTGAGATGAAGGTTCAGGGAAGTGATTGACA137130.34143493302890604No Hit
CTTGAGGACCGACTCCTGCTGGGCATTGATAGCCACAGAGGGCTTCAGAG124380.30968917793433487No Hit
AGCGAAAGCAGGGGGAGTAGCAGAGGGACAGGAAGCAGCAGACTCCCAGC119810.29831050336318266No Hit
CAGTAGGTTAAGTGTCTGCCTTCAGTTCAGGTCTTGATCTCCGGGTCCTG117970.2937291551769857No Hit
GGACTCTAAAGTGACTTTCCTGACGGGTTAGCTCTGAAGCCCTCTGTGGC110620.2754286610636446No Hit
GTGGCTATCAATGCCCAGCAGGAGTCGGTCCTCAAGTAGCAAACACTCTG108350.2697766717252386No Hit
CTCTGTGGCTATCAATGCCCAGCAGGAGTCGGTCCTCAAGTAGCAAACAC97870.24368290596907344No Hit
GTTCAGGTCTTGATCTCCGGGTCCTGGGATGGAACCCCTTGTTAGGCTCC95190.23701007274135177No Hit
CTCTAAAGTGACTTTCCTGACGGGTTAGCTCTGAAGCCCTCTGTGGCTAT94950.2364125055866304No Hit
AGCAGGGGGAGTAGCAGAGGGACAGGAAGCAGCAGACTCCCAGCTGAGCA93420.23260301497528188No Hit
GGCCATGTTAGGTTTAATCAGGACCTGGTTTTCAGTGAGATGAAGGTTCA92750.23093480666835148No Hit
TGTTAGGTTTAATCAGGACCTGGTTTTCAGTGAGATGAAGGTTCAGGGAA91950.22894291615261367No Hit
GCAGGAGTCGGTCCTCAAGTAGCAAACACTCTGGAGAAACAGCATTTTTG91610.22809636268342512No Hit
GGTTCAGGGAAGTGATTGACAAAAATGCTGTTTCTCCAGAGTGTTTGCTA90670.22575589132743318No Hit
AAAGCAGGGGGAGTAGCAGAGGGACAGGAAGCAGCAGACTCCCAGCTGAG87630.21818670736762952No Hit
GGGTGGGGGAATGCCTGGGGGCTCAGTAGGTTAAGTGTCTGCCTTCAGTT87440.21771363337014182No Hit
GGTAAATATATTTTTAAAAACTTTACTCTGGGGCGCCTGGGTGGCTCAGT86780.21607032369465812No Hit
GTAGCAAACACTCTGGAGAAACAGCATTTTTGTCAATCACTTCCCTGAAC85030.2117130631914817No Hit
CCTCTGTGGCTATCAATGCCCAGCAGGAGTCGGTCCTCAAGTAGCAAACA82990.20663374237635027No Hit
GTGTTTGCTACTTGAGGACCGACTCCTGCTGGGCATTGATAGCCACAGAG82900.20640965469332978No Hit
GGGAGTAGCAGAGGGACAGGAAGCAGCAGACTCCCAGCTGAGCAGGGAGC82650.20578718890716172No Hit
GTAGCAGAGGGACAGGAAGCAGCAGACTCCCAGCTGAGCAGGGAGCCTAA81660.20332222439393616No Hit
AGCAAACACTCTGGAGAAACAGCATTTTTGTCAATCACTTCCCTGAACCT81420.20272465723921484No Hit
GCAGAGGGACAGGAAGCAGCAGACTCCCAGCTGAGCAGGGAGCCTAACAA80840.20128053661530496No Hit
CTGAAGCCCTCTGTGGCTATCAATGCCCAGCAGGAGTCGGTCCTCAAGTA78040.19430891981022264No Hit
ACCCCAGGCCATGTTAGGTTTAATCAGGACCTGGTTTTCAGTGAGATGAA75410.18776057973973462No Hit
CTTCAGTTCAGGTCTTGATCTCCGGGTCCTGGGATGGAACCCCTTGTTAG74800.18624176322148453No Hit
TCAGGGAAGTGATTGACAAAAATGCTGTTTCTCCAGAGTGTTTGCTACTT74280.18494703438625495No Hit
GGACCTGGTTTTCAGTGAGATGAAGGTTCAGGGAAGTGATTGACAAAAAT73830.18382659597115245No Hit
GCCTAACAAGGGGTTCCATCCCAGGACCCGGAGATCAAGACCTGAACTGA73090.18198409724409498No Hit
GGGAAGTGATTGACAAAAATGCTGTTTCTCCAGAGTGTTTGCTACTTGAG72750.1811375437749064No Hit
CCTGGGGGCTCAGTAGGTTAAGTGTCTGCCTTCAGTTCAGGTCTTGATCT71430.17785092442393904No Hit
CAGCAGGAGTCGGTCCTCAAGTAGCAAACACTCTGGAGAAACAGCATTTT69970.17421572423271756No Hit
TTCCTGACGGGTTAGCTCTGAAGCCCTCTGTGGCTATCAATGCCCAGCAG69370.1727218063459142No Hit
GCCCAGCAGGAGTCGGTCCTCAAGTAGCAAACACTCTGGAGAAACAGCAT68080.16950988288928698No Hit
CAAGTAGGCAGAGAGGCAGGCAGAGAGAGAGAGGAGGAAGCAGGCTCCCT66690.16604897311819256No Hit
GTTTCTCCAGAGTGTTTGCTACTTGAGGACCGACTCCTGCTGGGCATTGA65840.16393258944522113No Hit
CTGTGGCTATCAATGCCCAGCAGGAGTCGGTCCTCAAGTAGCAAACACTC65020.1618909016665899No Hit
GTTTGCTACTTGAGGACCGACTCCTGCTGGGCATTGATAGCCACAGAGGG63430.15793201926656103No Hit
ACTCCCAGCTGAGCAGGGAGCCTAACAAGGGGTTCCATCCCAGGACCCGG62940.15671198632567163No Hit
ATCAATGCCCAGCAGGAGTCGGTCCTCAAGTAGCAAACACTCTGGAGAAA61270.15255391487406894No Hit
TTGTTAGGCTCCCTGCTCAGCTGGGAGTCTGCTGCTTCCTGTCCCTCTGC60680.15108489561871233No Hit
ACTCTGGGGCGCCTGGGTGGCTCAGTGGGTTATGCCGCTGCCTTCGGCTC59500.14814685710799907No Hit
ACTTTACTCTGGGGCGCCTGGGTGGCTCAGTGGGTTATGCCGCTGCCTTC59310.14767378311051133No Hit
CAAAAATGCTGTTTCTCCAGAGTGTTTGCTACTTGAGGACCGACTCCTGC58910.14667783785264243No Hit
GGACAGGAAGCAGCAGACTCCCAGCTGAGCAGGGAGCCTAACAAGGGGTT58680.14610516932936782No Hit
CTCCCAGCTGAGCAGGGAGCCTAACAAGGGGTTCCATCCCAGGACCCGGA58090.14463615007401118No Hit
TCCCTGAACCTTCATCTCACTGAAAACCAGGTCCTGATTAAACCTAACAT57410.14294304313563405No Hit
GGTCTTGATCTCCGGGTCCTGGGATGGAACCCCTTGTTAGGCTCCCTGCT57200.14242017187525288No Hit
GGGAATGCCTGGGGGCTCAGTAGGTTAAGTGTCTGCCTTCAGTTCAGGTC56490.14065236904253559No Hit
GTCTTGATCTCCGGGTCCTGGGATGGAACCCCTTGTTAGGCTCCCTGCTC55340.13778902642616248No Hit
GGGTTATGCCGCTGCCTTCGGCTCGGGTCATGATCTCAGGGTCCTGGGAT55120.13724125653433458No Hit
CCTGACGGGTTAGCTCTGAAGCCCTCTGTGGCTATCAATGCCCAGCAGGA54800.13644450032803948No Hit
CTCCAGAGTGTTTGCTACTTGAGGACCGACTCCTGCTGGGCATTGATAGC54660.13609591948778535No Hit
GGAAGCAGCAGACTCCCAGCTGAGCAGGGAGCCTAACAAGGGGTTCCATC54650.13607102085633865No Hit
GACAGAGAGAGACCACAAGTAGGCAGAGAGGCAGGCAGAGAGAGAGAGGA54340.13529916328149022No Hit
GTCCTGGGATGGAACCCCTTGTTAGGCTCCCTGCTCAGCTGGGAGTCTGC53520.133257475502859No Hit
GCCTGGGGGCTCAGTAGGTTAAGTGTCTGCCTTCAGTTCAGGTCTTGATC53390.13293379329405158No Hit
GGCTATCAATGCCCAGCAGGAGTCGGTCCTCAAGTAGCAAACACTCTGGA52880.13166396309026873No Hit
TCCCAGGACCCGGAGATCAAGACCTGAACTGAAGGCAGACACTTAACCTA51100.12723200669275214No Hit
TAACAAGGGGTTCCATCCCAGGACCCGGAGATCAAGACCTGAACTGAAGG49810.12402008323612493No Hit
ATCAGGACCTGGTTTTCAGTGAGATGAAGGTTCAGGGAAGTGATTGACAA49640.12359680650153064No Hit
GTTAAGTGTCTGCCTTCAGTTCAGGTCTTGATCTCCGGGTCCTGGGATGG48300.12026038988766984No Hit
GCCTTCAGTTCAGGTCTTGATCTCCGGGTCCTGGGATGGAACCCCTTGTT48160.11991180904741572No Hit
GCGAAAGCAGGACTCTAAAGTGACTTTCCTGACGGGTTAGCTCTGAAGCC45630.11361245529139491No Hit
GTGACTTTCCTGACGGGTTAGCTCTGAAGCCCTCTGTGGCTATCAATGCC45550.11341326623982113No Hit
GCAGGGGGAGTAGCAGAGGGACAGGAAGCAGCAGACTCCCAGCTGAGCAG45550.11341326623982113No Hit
GTGTCTGCCTTCAGTTCAGGTCTTGATCTCCGGGTCCTGGGATGGAACCC45130.1123675237190588No Hit
AGGTTAAGTGTCTGCCTTCAGTTCAGGTCTTGATCTCCGGGTCCTGGGAT44760.11144627435553005No Hit
GCTGAGCAGGGAGCCTAACAAGGGGTTCCATCCCAGGACCCGGAGATCAA44620.11109769351527593No Hit
GGTCATGATCTCAGGGTCCTGGGATCGAGTCCCGCATCGGGCTCTTTGCT43790.10903110710519796No Hit
GGCAGAGGGAGAGGGGGAAGCAGGCTTCGGGCTGAGCAAGGAGCCGGATA43210.10758698648128805No Hit
GGAGAAACAGCATTTTTGTCAATCACTTCCCTGAACCTTCATCTCACTGA43000.10706411522090689No Hit
GGGGAATGCCTGGGGGCTCAGTAGGTTAAGTGTCTGCCTTCAGTTCAGGT42930.10688982480077983No Hit
GTCCTGGGATCGAGTCCCGCATCGGGCTCTTTGCTCAGCAGGGAGCCTGC42800.10656614259197243No Hit
GTTCCATCCCAGGACCCGGAGATCAAGACCTGAACTGAAGGCAGACACTT41630.1036530027127059No Hit
GGGCTCAGTAGGTTAAGTGTCTGCCTTCAGTTCAGGTCTTGATCTCCGGG40850.10171090945986154No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)