FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_G6a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_G6a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1367340
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCAAAG157871.154577500841049No Hit
ACATCTAGATGATCAAATAGTCCTTACTTATAACTAAACCACCTGAATCA113010.8264952389310634No Hit
CCCTTATTTCTCTGTAATCATCATCAAGGGGCTAAGGTATCAGTTCTCAA80050.585443269413606No Hit
TCTCAGAAGAGTATCAAGAACAATTCAGAACATACAATATGTGTGTGATA77430.5662819781473518No Hit
GGATATTACTCTACATCTAGATGATCAAATAGTCCTTACTTATAACTAAA76170.5570670060116722No Hit
GTCCTTACTTATAACTAAACCACCTGAATCACCTATGATTTGAGGTACTA69450.507920487954715No Hit
CTTATAACTAAACCACCTGAATCACCTATGATTTGAGGTACTACGTGTTA64090.4687202890283324No Hit
ACTTATAACTAAACCACCTGAATCACCTATGATTTGAGGTACTACGTGTT62790.45921277809469485No Hit
ATCAAATAGTCCTTACTTATAACTAAACCACCTGAATCACCTATGATTTG57830.422937967147893No Hit
GTATGATAAACTTGAGTTACACTGAATTTTACTCAGTTCCCACAGAAGTA56880.41599017069638866No Hit
TAACTAAACCACCTGAATCACCTATGATTTGAGGTACTACGTGTTAGGAA53620.3921482586628051No Hit
GGATATGATTGCATAATTAAGGAAGTTTGGGTTTTTTTAAGAGTACTTCT53040.38790644609241304No Hit
ATTCAGAACATACAATATGTGTGTGATAACCACCTACTGGATGAATATTT49880.36479588105372474No Hit
ATCAAGAACAATTCAGAACATACAATATGTGTGTGATAACCACCTACTGG44230.32347477584214607No Hit
CAAATAACACTGTATGATAAACTTGAGTTACACTGAATTTTACTCAGTTC43490.3180628080799216No Hit
ATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCAAAGAGAG41510.30358213758099667No Hit
CAATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCAAAGAG39400.288150715988708No Hit
ATATAGGATTTGCTAAATGACCCAGAATGAGAGGAGATAAATCATAATCA35430.25911624029136865No Hit
CTCTACATCTAGATGATCAAATAGTCCTTACTTATAACTAAACCACCTGA34060.2490967864613044No Hit
CCTATGATTTGAGGTACTACGTGTTAGGAAATAAAGGCTCATCTTGAGAA33040.24163704711337342No Hit
ACTCTACATCTAGATGATCAAATAGTCCTTACTTATAACTAAACCACCTG32910.24068629602000965No Hit
GTTCTCAAGATGAGCCTTTATTTCCTAACACGTAGTACCTCAAATCATAG30750.22488920093027337No Hit
AAATAACACTGTATGATAAACTTGAGTTACACTGAATTTTACTCAGTTCC27520.20126669299515848No Hit
ATTAAGGAAGTTTGGGTTTTTTTAAGAGTACTTCTGTGGGAACTGAGTAA27510.20119355829566898No Hit
TACTAATACAGACCAGGGAAAGAAGGAGGAGGGAGAGGGGATGAGAAGAG26410.19314874135182178No Hit
GATTATAAAAGGTCACCAAAAAGGACAAAAAACATTAAAAAGCAATGTCC25560.1869322918952126No Hit
GTTTTTAAGTGTACAGTTGAGTGATTTTTAATCAGTTTGAGTATGCAGCT25250.18466511621103748No Hit
ATATAATTCCAAACTTCAATCAGTAATTCCAATGTGAAGGATGACTCAGT25100.1835680957186947No Hit
CATCTAGATGATCAAATAGTCCTTACTTATAACTAAACCACCTGAATCAC24540.1794725525472816No Hit
CTGTATGATAAACTTGAGTTACACTGAATTTTACTCAGTTCCCACAGAAG24430.17866807085289688No Hit
AAATAGTCCTTACTTATAACTAAACCACCTGAATCACCTATGATTTGAGG23860.17449939298199424No Hit
TTACTCTACATCTAGATGATCAAATAGTCCTTACTTATAACTAAACCACC21810.15950677958664267No Hit
TTATTAACCAGGATATTACTCTACATCTAGATGATCAAATAGTCCTTACT21470.157020199803999No Hit
ACTTTATGCTTTAAGAAAGTTTTGGGAAGTTTCTGATAAAATATTGGTTC21010.1536560036274811No Hit
TATATAACCAGAACTAAAAATCAGGTATCATTCTTGTCCCATTTACTAAA20700.15138882794330596No Hit
ATATAAAACAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATACACAC20420.14934105635759942No Hit
GTGTACAGTTGAGTGATTTTTAATCAGTTTGAGTATGCAGCTATCATCAC20330.14868284406219376No Hit
GTATCAAGAACAATTCAGAACATACAATATGTGTGTGATAACCACCTACT19760.14451416619129112No Hit
TCCTAACACGTAGTACCTCAAATCATAGGTGATTCAGGTGGTTTAGTTAT19370.14166191291119984No Hit
GTACTACGTGTTAGGAAATAAAGGCTCATCTTGAGAACTGATACCTTAGC19330.14136937411324177No Hit
GTGTTAGGAAATAAAGGCTCATCTTGAGAACTGATACCTTAGCCCCTTGA19330.14136937411324177No Hit
GTATATAATTCCAAACTTCAATCAGTAATTCCAATGTGAAGGATGACTCA19140.1399798148229409No Hit
GTATTATATAGGATTTGCTAAATGACCCAGAATGAGAGGAGATAAATCAT18350.13420217356326883No Hit
TTCTAGGACATTGCTTTTTAATGTTTTTTGTCCTTTTTGGTGACCTTTTA18110.13244694077552036No Hit
CCATTAACTGGATATGATTGCATAATTAAGGAAGTTTGGGTTTTTTTAAG17870.13069170798777188No Hit
GAACATACAATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGG17850.13054543858879283No Hit
GAAATAAGGGAGGACCTCTCTTTGCCTCCAAATATTCATCCAGTAGGTGG17330.12674243421533782No Hit
CATACAATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCAA17120.1252066055260579No Hit
ACACTGTATGATAAACTTGAGTTACACTGAATTTTACTCAGTTCCCACAG16630.12162300525107143No Hit
GATTTGAGGTACTACGTGTTAGGAAATAAAGGCTCATCTTGAGAACTGAT16400.11994090716281247No Hit
CTGTAATCATCATCAAGGGGCTAAGGTATCAGTTCTCAAGATGAGCCTTT16050.11738119268067929No Hit
ATATTACTCTACATCTAGATGATCAAATAGTCCTTACTTATAACTAAACC15790.11547969049395176No Hit
AATATTAACAGCCTGAAAAGGATTCTTGATTACTACCATTCATCAACTAT15670.11460207410007753No Hit
CTTGTAAACCTGGAATAATGTTCCATTAACTGGATATGATTGCATAATTA15490.11328564950926617No Hit
AATGTATATAATTCCAAACTTCAATCAGTAATTCCAATGTGAAGGATGAC15490.11328564950926617No Hit
TCTCTAATGTATCAGCCACTGAGTCATCCTTCACATTGGAATTACTGATT15400.1126274372138605No Hit
GGGCTAAGGTATCAGTTCTCAAGATGAGCCTTTATTTCCTAACACGTAGT15340.11218862901692338No Hit
AACCAGGATATTACTCTACATCTAGATGATCAAATAGTCCTTACTTATAA15250.1115304167215177No Hit
TATTTATACCTGTCTTTCCACATACTTTTTTCAAATGGTTTCATGAAAAG15080.11028712683019586No Hit
GTTCTTATACTATAGGAACAAAAACTACTGCCACAGAATGTAATGCTACT14810.10831248994397882No Hit
ACATACAATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCA14620.10692293065367793No Hit
GAACAATTCAGAACATACAATATGTGTGTGATAACCACCTACTGGATGAA14550.1064109877572513No Hit
GGTATCAGTTCTCAAGATGAGCCTTTATTTCCTAACACGTAGTACCTCAA14320.10472888966899234No Hit
ATTTTACTCAGTTCCCACAGAAGTACTCTTAAAAAAACCCAAACTTCCTT14290.1045094855705238No Hit
GAATGAAGGGTGACTGCAAATGAGCACAAAGATCATTCTGGGATGATGAA14200.1038512732751181No Hit
ATGATAAACTTGAGTTACACTGAATTTTACTCAGTTCCCACAGAAGTACT14090.1030467915807334No Hit
ATTCTGGGATGATGAAAATGTTCTAAAAATAAACTGTGATGATAGCTGCA14090.1030467915807334No Hit
CTTGAGAACTGATACCTTAGCCCCTTGATGATGATTACAGAGAAATAAGG13970.10216917518685917No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)