FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_G5a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_G5a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences951528
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC18950.19915336175078402No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT17820.18727772593134412No Hit
GTGTATTGAAGCATTAGACAGATAAAGCCAGTAGCCCAAGGTCACACAAT16950.17813453729159837No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG15060.15827174817766793No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT14930.15690552458782087No Hit
TACCAGAAGTGCTGCAATCTATTCGAGAAATTTTTCCCTAGCAGTTCATA13580.14271781807787054No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT13380.140615935631952No Hit
GTCCTAATCTCACCTGGGGGCTATTTCTAGTAGACTCCTGGTGGAGCCTT9890.10393808695067303No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG9750.10246676923853003No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)