FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_G2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_G2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5271146
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAATGAAATGTTCAAATAAGGAAAGATGAAGTTCATATGGTGCATGG149890.28435941633944495No Hit
TCCTAATACAGTTCCGATCTTATTAGTTAAGACAGAATCTGTCTGTTCCA146440.2778143500483576No Hit
ATCTAGAAGTCTATTCCTCAAAAGTATAAGATTAGCAGATACAAGACCTC143080.27144002461703776No Hit
TCATTGAACTGCTCACTGAGTCCAGTGAAGCTTCAAAGAATGCCAAATTA140420.2663936836505762No Hit
GTTTATTTCTTCTACTTTACCAACGTTTGCAGTTTGTGAGAAATTTGTAG139740.2651036415989995No Hit
GTATAAGATTAGCAGATACAAGACCTCCAAACTCTTCTTCAGTTCCAGTA128880.24450091118705494No Hit
CCATATGAACTTCATCTTTCCTTATTTGAACATTTCATTGAACTGCTCAC117860.22359464146885707No Hit
TCACTGGACTCAGTGAGCAGTTCAATGAAATGTTCAAATAAGGAAAGATG113380.2150955408937639No Hit
CTGTTGATAGTGGGCATGAGACCTCCATTATTCCAAATTCAACTGCTTTC109780.20826590650306404No Hit
GTCCAGTGAAGCTTCAAAGAATGCCAAATTAATGAGGGAATTCCAGCTAA108520.2058755344663191No Hit
GCATACATACGGCCTGAAAGCTTCCAACATAATTTGGTCCTAGTTCATAA105200.19957709386156253No Hit
CTTTAGATGTGTAAACTAGTTTTAGCAAGATATCTAGAAGTCTATTCCTC105000.1991976697287459No Hit
GTCTAAGGGAAAGCCGTGACCCCGGGGTCCAGCAGGTGCCTGCCCTGTGC98970.18775803212432363No Hit
TCCATAAACATCATAATCCAGTCAAAACCCAGTGTCCTGACCAGTTCTTC98840.1875114064379928No Hit
ATACAAGACCTCCAAACTCTTCTTCAGTTCCAGTATCAAGCTTCTCTTCA97330.18464675423522703No Hit
CTTCTAGATATCTTGCTAAAACTAGTTTACACATCTAAAGAAAAGACAAG94890.1800177798148638No Hit
GAAATAAACAATGAAGAGAAGCTTGATACTGGAACTGAAGAAGAGTTTGG94690.17963835568204714No Hit
GTTTATTTCCATAACTACAAATTTCTCACAAACTGCAAACGTTGGTAAAG92310.1751232085015289No Hit
ACTCATAGCAGCAATGGTAGGTTGGGATAAAGACATATCTCGAAGAGTCA89760.1702855508081165No Hit
GTTCTTCACAAGCTTGCAAATTAATGCTTGTCTTTTCTTTAGATGTGTAA87920.16679484878620324No Hit
CCATTATTCCAAATTCAACTGCTTTCCAGGATCTACTTTGTGATTTTGAA83230.15789735287165257No Hit
TATGTATGCCGTGTAAAGATACAAAGTCTGAAGGCGTAGTCCCATCTCCT83120.15768866959860342No Hit
CTATCAACAGTTCCCACCAAGGAAAATGTTAGATGGAGAATGTGGCTGTT82640.15677805167984343No Hit
GAATAAGCCTGGCCTCAGTTTTTCTGACGCGACAGAGGTTTTGGGCACTC82330.15618994427397762No Hit
GTCCTGACCAGTTCTTCACAAGCTTGCAAATTAATGCTTGTCTTTTCTTT79520.15085903520790356No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT77980.1479374693852153No Hit
ATCTTATACTTTTGAGGAATAGACTTCTAGATATCTTGCTAAAACTAGTT75750.14370689030430953No Hit
GTCTATGCGTACATTGGCACTCCACCTGCCCAGCGTCAAATTGCTTCCTT75400.14304289807188036No Hit
GTGTAAACTAGTTTTAGCAAGATATCTAGAAGTCTATTCCTCAAAAGTAT75160.14258758911250038No Hit
GGATAAAGACATATCTCGAAGAGTCAGTAGCAATTTGGGGATTAGCTGGA74660.14163902878045875No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT73690.13979882173629796No Hit
GTCTTGTATCTGCTAATCTTATACTTTTGAGGAATAGACTTCTAGATATC72520.1375791905593205No Hit
TTACCAACGTTTGCAGTTTGTGAGAAATTTGTAGTTATGGAAATAAACAA70950.1346007111167097No Hit
GCTCAGGTTTGGCCAGTTTATTTCTTCTACTTTACCAACGTTTGCAGTTT70040.13287433131239393No Hit
GTATCAAGCTTCTCTTCATTGTTTATTTCCATAACTACAAATTTCTCACA67850.1287196370580515No Hit
ACTATAAGTTCTCCACATCGAAAGCGGGCACAGCACGGGAGAATCTCATA67620.12828329930531235No Hit
GTTCATATGGTGCATGGAGCCAAACTTCAAAATCACAAAGTAGATCCTGG66530.12621543778146158No Hit
CTCCTTAACAGCCACATTCTCCATCTAACATTTTCCTTGGTGGGAACTGT66320.12581704244200406No Hit
GCACGGGAGAATCTCATAAAAAGAAGATTCTTCACTAAAATCATCAACAT64990.1232938719587733No Hit
AATTAATGCTTGTCTTTTCTTTAGATGTGTAAACTAGTTTTAGCAAGATA64920.12316107351228746No Hit
ATATGAACTTCATCTTTCCTTATTTGAACATTTCATTGAACTGCTCACTG64150.12170029060094333No Hit
GATATCTAGAAGTCTATTCCTCAAAAGTATAAGATTAGCAGATACAAGAC63310.12010670924311335No Hit
GGTTTGGCCAGTTTATTTCTTCTACTTTACCAACGTTTGCAGTTTGTGAG63100.11970831390365586No Hit
CATCTAAAGAAAAGACAAGCATTAATTTGCAAGCTTGTGAAGAACTGGTC62760.11906329287786756No Hit
GGATAGACTGATTACTCAGTAGCACAACAAGAATCCTCATGGCTGCCGTA62730.11900637925794505No Hit
TCATAATCCAGTCAAAACCCAGTGTCCTGACCAGTTCTTCACAAGCTTGC62610.11877872477825506No Hit
AACCTGAGCAGATCATTGCTGTTGGGAAAACCTTGTAGTAAGAAGCTCAG62420.11841827185207923No Hit
CGCATAGACTGTGCTGACCACAGGTGGGTTAGCGGAACCTGAGCCACCAG61380.11644526636143261No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA60770.1152880227563418No Hit
AGTTTACACATCTAAAGAAAAGACAAGCATTAATTTGCAAGCTTGTGAAG59820.11348575812546266No Hit
GTTTTGGAGTAGTTCCTCTTTGGTGGAAACAATCAGAGATCGATCTTTTG58920.11177834952778769No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG58580.11113332850199936No Hit
GAGTAATGTGCTGAGCTTCTTACTACAAGGTTTTCCCAACAGCAATGATC58230.11046933626957021No Hit
CTAGATATCTTGCTAAAACTAGTTTACACATCTAAAGAAAAGACAAGCAT57870.10978637283050025No Hit
GCTATGAGTAATGTGCTGAGCTTCTTACTACAAGGTTTTCCCAACAGCAA56470.10713040390078361No Hit
GTATTAGGATTCAATGTGGGCAGGAGTGCTGGCGGGAGATCGACGGTCAG56290.10678892218124864No Hit
GATTAGCAGATACAAGACCTCCAAACTCTTCTTCAGTTCCAGTATCAAGC56010.1062577283953053No Hit
GCATTACGTGTGCCTTGCCATTGTCTTATCCGCAAAAGATCGATCTCTGA55920.10608698753553782No Hit
TCTTACTACAAGGTTTTCCCAACAGCAATGATCTGCTCAGGTTTGGCCAG55540.10536608168318616No Hit
CCTCAAAAGTATAAGATTAGCAGATACAAGACCTCCAAACTCTTCTTCAG54230.10288085361323705No Hit
ATGGTAGGTTGGGATAAAGACATATCTCGAAGAGTCAGTAGCAATTTGGG53660.10179949483470957No Hit
ATCTAACATTTTCCTTGGTGGGAACTGTTGATAGTGGGCATGAGACCTCC53260.10104064656907626No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)