FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_G1a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_G1a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1740248
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT112450.6461722696994912No Hit
GGCATAGGGAGTGTCCTTATTGCTAAAAAGGATTCTGTCTCCTTCAAAGA108620.6241639122699754No Hit
AACAAGTACTCTGGACCAAAAATAAAACTTCTTTGAAGGAGACAGAATCC67070.38540483885055465No Hit
AGTCAGGAAACAAGTACTCTGGACCAAAAATAAAACTTCTTTGAAGGAGA66340.38121003443187407No Hit
AGCAGGGACAGAGCTGAGAAACTGATCTCTGCTCTCTCCCTGCCCAGTCC65930.3788540483885055No Hit
CCCTATGCCATTGGGCAAGGGGCATTAGCCTGCAGAAAGGATCTTGCTGT63130.3627643876045253No Hit
TAACTATACTGTTAGAGTGAAGTTCAAGTATTCCACGCTTTTCTAGCAAG60540.3478814513793436No Hit
AAACAAGGGAGTTTGGAGCCATAGGCATAGTAAAAGCTGGGATTATGGGC59650.3427672377730071No Hit
CCTTAGTACAACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCC58670.337135856498614No Hit
GTCCTGGGTGGCAGTCCTATCGGGGCACCATAGGGGCAATTGCAGCCTGT56900.32696489236016935No Hit
GTATAACTATACTGTTAGAGTGAAGTTCAAGTATTCCACGCTTTTCTAGC56360.32386188635183033No Hit
TCTATACACTGAAAACTAGAAAACACTGATGAAAGAAATTGAAGATGGCA54250.3117371776896167No Hit
CAAGTACTCTGGACCAAAAATAAAACTTCTTTGAAGGAGACAGAATCCTT50740.29156763863541285No Hit
ATGTAAAGAAGCTTAAAACCACTTACCTAAAGTCTTGCTTTATAATCAGT49910.28679820347444734No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA46820.2690421135378406No Hit
ACTATACTGTTAGAGTGAAGTTCAAGTATTCCACGCTTTTCTAGCAAGCA46250.26576671830681603No Hit
CTCCCTGCCCAGTCCTCTTCCCATCCTCATCCACAGGCTGCAATTGCCCC45650.262318933853106No Hit
GAACTAGAACAAATAATCCTAAAATTTGTATGGAACCACAAAAGAGCCTG45630.26220400770464897No Hit
AATCTATACACTGAAAACTAGAAAACACTGATGAAAGAAATTGAAGATGG44500.2557106803168284No Hit
AAATAAAACTTCTTTGAAGGAGACAGAATCCTTTTTAGCAATAAGGACAC40600.23330008136771307No Hit
GCATAAACTATCTGGTGTTTTTAAAATCTGCAATATTGAGATGGAGAACT40350.23186350451200058No Hit
GCATAAAGCCAAGTAACAAATTGCTACCTTCAACAAGCAGAGCTAGACTC38900.22353135874886798No Hit
GCCATAGGCATAGTAAAAGCTGGGATTATGGGCCACAGTCCTGGGTGGCA38870.2233589695261825No Hit
GTTTGGAGCCATAGGCATAGTAAAAGCTGGGATTATGGGCCACAGTCCTG38200.21950894355287293No Hit
TAGTACAACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCCAGA37400.21491189761459287No Hit
TTTCTGATGAGAGTGAGCACTGGTTTCACTGGTTTTTCATGATGTAAAGA36190.2079588656329443No Hit
GAATAAAACTGTCAAAAAATAAAGAAAGTGACTTAGAGCAGGATCCATAA35690.20508571192151925No Hit
CTGTATAACTATACTGTTAGAGTGAAGTTCAAGTATTCCACGCTTTTCTA34800.19997149831518266No Hit
ATATTGAGATGGAGAACTGATTATAAAGCAAGACTTTAGGTAAGTGGTTT32800.1884788834694825No Hit
GAGCTGAGAAACTGATCTCTGCTCTCTCCCTGCCCAGTCCTCTTCCCATC31910.18336466986314595No Hit
TCTTTACATCATGAAAAACCAGTGAAACCAGTGCTCACTCTCATCAGAAA31740.18238779760126142No Hit
AGTAGAAACAAGGGAGTTTGGAGCCATAGGCATAGTAAAAGCTGGGATTA31570.18141092533937692No Hit
ACTTAGAGCAGGATCCATAAATGGGCTTCTGGTCTCTTAATCTCTGAAAC31460.1807788315228634No Hit
GTCCTCTTCCCATCCTCATCCACAGGCTGCAATTGCCCCTATGGTGCCCC30970.17796314088566687No Hit
GCATAGGGAGTGTCCTTATTGCTAAAAAGGATTCTGTCTCCTTCAAAGAA30950.17784821473720988No Hit
GGAAACAAGTACTCTGGACCAAAAATAAAACTTCTTTGAAGGAGACAGAA30920.17767582551452438No Hit
CCCCCAAAATGCATAAAGCCAAGTAACAAATTGCTACCTTCAACAAGCAG30490.17520491332269883No Hit
ACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCCAGATTTGCTT30430.17486013487732782No Hit
AGCATAAACTATCTGGTGTTTTTAAAATCTGCAATATTGAGATGGAGAAC30300.17411311491235731No Hit
CTCTGAAACTGTACGCAAATTTGCATAATGTGCATTTTTCTGATGAGAGT29940.1720444442401313No Hit
CCCCTATGGTGCCCCGATAGGACTGCCACCCAGGACTGTGGCCCATAATC29670.1704929412359618No Hit
CCATAGGGGCAATTGCAGCCTGTGGATGAGGATGGGAAGAGGACTGGGCA29410.16899890130602074No Hit
GTATTTCTTTCTCAGTGTCATGAATTGTGGTTAATGCTTGCTAGAAAAGC29380.16882651208333524No Hit
ACCAATAACACTTCTCACAGAACTAGAACAAATAATCCTAAAATTTGTAT28710.16497648611002572No Hit
ACCCAGGACTGTGGCCCATAATCCCAGCTTTTACTATGCCTATGGCTCCA28310.16267796314088567No Hit
GTATGTTTGTTTTGACTCCTTTTAAAAAGAGTAGAGCATAAACTATCTGG27450.1577361387572346No Hit
GAGCTAGACTCGGTTTGCAGTTCTATCCTAAAACTCCTTTTCACCAAGCT27150.1560122465303796No Hit
GACTTAATATGTTGCAGAGAAGGTAGTGTTAATGAACCGAAGTTAGAGGT26700.15342640819009704No Hit
GGGCTAGCTGGACGAGTCAGGAAACAAGTACTCTGGACCAAAAATAAAAC26420.151817442111699No Hit
GGTCTGAGAAACGCAGGTGCCTAAGTTCCAGTCACCCCCAAAATGCATAA26320.151242811369414No Hit
GTAACAAATTGCTACCTTCAACAAGCAGAGCTAGACTCGGTTTGCAGTTC26150.1502659391075295No Hit
GTCATGAAGGCACCCCTTAGTACAACTTTGTAGTATAACTTGAAGTCTGG26100.149978623736387No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT25770.14808234228684647No Hit
GTACAACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCCAGATT25580.14699054387650495No Hit
ACTCTGGACCAAAAATAAAACTTCTTTGAAGGAGACAGAATCCTTTTTAG25540.14676069157959096No Hit
CTTCCCATCCTCATCCACAGGCTGCAATTGCCCCTATGGTGCCCCGATAG25500.14653083928267696No Hit
GTTCCAGTCACCCCCAAAATGCATAAAGCCAAGTAACAAATTGCTACCTT25380.14584128239193495No Hit
AGCTGGACGAGTCAGGAAACAAGTACTCTGGACCAAAAATAAAACTTCTT24770.1423360348639964No Hit
GAGTAGAGCATAAACTATCTGGTGTTTTTAAAATCTGCAATATTGAGATG24520.14089945800828388No Hit
GGTTAGGCCTTTAAGCTAAGCTTGGTGAAAAGGAGTTTTAGGATAGAACT24500.14078453185982687No Hit
GGACAGAGCTGAGAAACTGATCTCTGCTCTCTCCCTGCCCAGTCCTCTTC24210.13911810270720035No Hit
TTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCCAGATTTGCTTTCC23920.13745167355457383No Hit
GAGAAACGCAGGTGCCTAAGTTCCAGTCACCCCCAAAATGCATAAAGCCA23890.13727928433188832No Hit
CTAGAAAACACTGATGAAAGAAATTGAAGATGGCACAAACAAACGGGAAG23810.1368195797380603No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC23760.13653226436691782No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA23010.13222253379978027No Hit
GGCATAGTAAAAGCTGGGATTATGGGCCACAGTCCTGGGTGGCAGTCCTA22640.13009640005332573No Hit
GTCATGAATTGTGGTTAATGCTTGCTAGAAAAGCGTGGAATACTTGAACT22260.1279128032326427No Hit
ATCAAAATACCAATAACACTTCTCACAGAACTAGAACAAATAATCCTAAA22190.1275105617130432No Hit
GAGCATAAACTATCTGGTGTTTTTAAAATCTGCAATATTGAGATGGAGAA22030.1265911525253872No Hit
TAGTAAAAGCTGGGATTATGGGCCACAGTCCTGGGTGGCAGTCCTATCGG21880.12572920641195967No Hit
CTCTTAATCTCTGAAACTGTACGCAAATTTGCATAATGTGCATTTTTCTG21390.12291351577476313No Hit
ATGGAGAACTGATTATAAAGCAAGACTTTAGGTAAGTGGTTTTAAGCTTC21300.12239634810670665No Hit
GTAGAAACAAGGGAGTTTGGAGCCATAGGCATAGTAAAAGCTGGGATTAT21220.12193664351287863No Hit
CCACAAAAGAGCCTGAATAGCCAAACAACCTTAAAAAAGGAAAGCAAATC20780.1194082682468246No Hit
ATGGCATAGGGAGTGTCCTTATTGCTAAAAAGGATTCTGTCTCCTTCAAA20680.1188336375045396No Hit
TTGCAGTTCTATCCTAAAACTCCTTTTCACCAAGCTTAGCTTAAAGGCCT20520.11791422831688358No Hit
CCATAAATGGGCTTCTGGTCTCTTAATCTCTGAAACTGTACGCAAATTTG20480.11768437601996956No Hit
TTATAAAGCAAGACTTTAGGTAAGTGGTTTTAAGCTTCTTTACATCATGA20290.11659257760962805No Hit
AGCGAAAGCAGGGCTAGCTGGACGAGTCAGGAAACAAGTACTCTGGACCA20150.11578809457042905No Hit
CAATAACACTTCTCACAGAACTAGAACAAATAATCCTAAAATTTGTATGG20040.11515600075391554No Hit
CTTAATGACTGAGTCATGAAGGCACCCCTTAGTACAACTTTGTAGTATAA19990.11486868538277302No Hit
CTGTTAGAGTGAAGTTCAAGTATTCCACGCTTTTCTAGCAAGCATTAACC19880.11423659156625952No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT19870.11417912849203103No Hit
TAACAATATTGTTCTTCCAATCCATGAGCATGGATTGTCTTCCCGTTTGT19710.11325971930437499No Hit
TCCTAGTGATTAGGAAGTTCAGACTGTATTTCTTTCTCAGTGTCATGAAT19570.11245523626517599No Hit
CCAATAACACTTCTCACAGAACTAGAACAAATAATCCTAAAATTTGTATG19270.11073134403832097No Hit
GTATAACTTGAAGTCTGGAACTTGATACCTCCAGATTTGCTTTCCTTTTT18910.10866267336609495No Hit
GCTAAAAAGGATTCTGTCTCCTTCAAAGAAGTTTTATTTTTGGTCCAGAG18780.10791565340112444No Hit
AAGCTAAGCTTGGTGAAAAGGAGTTTTAGGATAGAACTGCAAACCGAGTC18740.10768580110421043No Hit
AATTTGCATAATGTGCATTTTTCTGATGAGAGTGAGCACTGGTTTCACTG18680.10734102265883942No Hit
GCCCAATGGCATAGGGAGTGTCCTTATTGCTAAAAAGGATTCTGTCTCCT18510.10636415039695492No Hit
ATCCATAAATGGGCTTCTGGTCTCTTAATCTCTGAAACTGTACGCAAATT18380.10561713043198441No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT18270.1049850366154709No Hit
GCAATATTGAGATGGAGAACTGATTATAAAGCAAGACTTTAGGTAAGTGG18100.1040081643535864No Hit
CCTTTAAGCTAAGCTTGGTGAAAAGGAGTTTTAGGATAGAACTGCAAACC18080.10389323820512938No Hit
AGATATCCCTTATAGGAAAAACCTCTAACTTCGGTTCATTAACACTACCT17960.10320368131438737No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA17950.10314621824015888No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA17910.10291636594324488No Hit
GTCCTTATTGCTAAAAAGGATTCTGTCTCCTTCAAAGAAGTTTTATTTTT17810.10234173520095985No Hit
CTTTTAAAAAGAGTAGAGCATAAACTATCTGGTGTTTTTAAAATCTGCAA17700.10170964138444635No Hit
AGTAAAAGCTGGGATTATGGGCCACAGTCCTGGGTGGCAGTCCTATCGGG17700.10170964138444635No Hit
GTTCTATCCTAAAACTCCTTTTCACCAAGCTTAGCTTAAAGGCCTAACCA17680.10159471523598934No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)