FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_F9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_F9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3922585
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGAACTGAGAGCCACTTCTATCTAGAAGTCATTTTCTGTAACGAAC182050.46410721501254915No Hit
GCCCTAAGAAGCCATGTGAAATTTAAGAGTAACCATTTAACTACAAGATA146500.3734782037865336No Hit
ATTTAAGAGTAACCATTTAACTACAAGATATGCAAAAACCTTCTGAAATA138000.3518088199490897No Hit
CCATTTAACTACAAGATATGCAAAAACCTTCTGAAATACTCGGGAGTATG137330.3501007626348441No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA119010.30339686711696495No Hit
TTATAAAAGTGGCTATGTAGTATTGTTAGTTTTGTCTCTAGATAAAGTTT113550.289477474675501No Hit
CTTCTATCTAGAAGTCATTTTCTGTAACGAACTAAAATTCCTCAGCTTGC112440.2866477080802583No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT112190.2860103732615099No Hit
GATTAGGACATTCCCCTGCTCCAAACCCTCCAGGGGCTCCCATTACAGTT109490.27912715721902776No Hit
ACTCAATACTCAGTAAGTTCAGGGATGTTTGGAAAGCCGGCCCTGCCTTT106900.27252436849679484No Hit
GTAGTATTGTTAGTTTTGTCTCTAGATAAAGTTTATTGTCTCGCCTTTTA101660.2591658306958294No Hit
ATGTAGTATTGTTAGTTTTGTCTCTAGATAAAGTTTATTGTCTCGCCTTT100160.2553418217833393No Hit
GTGTCAGGACTTGGCAACCTCTTCTGTAAAGGACCGGATAGTAAATACTT98850.2520021873330979No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA96620.24631716074986265No Hit
GTTATAAGGTGGAGAAGTGGGGAACGCAGTGAACCAGAGAGGGAAGGCAC92600.23606881686438919No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT88120.22464777691241872No Hit
GTCCTGACACAGGGCACTGAGTTTTATTCTAACTGTAATGGGAGCCCCTG87130.22212393103017525No Hit
GTCCAAGTCTGGCCCACCACTTGTTCTTGTAAACCAAGTTTTATTGGAAC86930.22161406317517657No Hit
GTACGTTAGGAGTCTTTCCAAGTTCTTTTTTGTGTGTCCTTTAACTAGTT83370.21253841535620005No Hit
CCTTATAACTTTCCTCAATTTAGGGAGTGGAGTTGATCCTCATCTTTGCC79540.20277444593297533No Hit
GTTCAGGGATGTTTGGAAAGCCGGCCCTGCCTTTGACGAGTTTGTTCCCT78620.20042905379998138No Hit
CCCTTACAAATTGTCTGTGGCTGCTTTTGTGCTACAACAGCAGAACCAAA78070.19902691719873503No Hit
ATCTAGAAGTCATTTTCTGTAACGAACTAAAATTCCTCAGCTTGCATACT76600.19527938846449472No Hit
CTACAAGATATGCAAAAACCTTCTGAAATACTCGGGAGTATGCAAGCTGA74990.19117495223175532No Hit
CATTTGGGTGGTACGTTAGGAGTCTTTCCAAGTTCTTTTTTGTGTGTCCT71830.1831190401227762No Hit
CCTTTAACTAGTTGATGCCTAGTGGAAATCGGCCTTGTTACCAATTTAGA70960.18090111495353192No Hit
GTATTGAGTTGGGGGCAGAGCCCAGTGGGAGAGGCGTCAAGTGCATCCCA67860.17299816320105235No Hit
CGTTAGGAGTCTTTCCAAGTTCTTTTTTGTGTGTCCTTTAACTAGTTGAT67610.17236082838230402No Hit
CTAAGAAGCCATGTGAAATTTAAGAGTAACCATTTAACTACAAGATATGC65670.1674151101888168No Hit
ACTCGGGAGTATGCAAGCTGAGGAATTTTAGTTCGTTACAGAAAATGACT65340.166573828228069No Hit
GTTCTAAATTGGTAACAAGGCCGATTTCCACTAGGCATCAACTAGTTAAA63710.16241840520982972No Hit
CCCTAAATTGAGGAAAGTTATAAGGTGGAGAAGTGGGGAACGCAGTGAAC62910.160378933789835No Hit
CTCCATGTTTGAGGAGTGAGTGAGTGAATAAACTAGCCATGTTCCATGAA62860.16025146682608535No Hit
TCCCTGAACTTACTGAGTATTGAGTTGGGGGCAGAGCCCAGTGGGAGAGG61130.15584110988034675No Hit
GCTCCAAACCCTCCAGGGGCTCCCATTACAGTTAGAATAAAACTCAGTGC60370.15390361203135178No Hit
CCCCAGTGCCTGGGAAGTGGTTGCACGTGCTCTCCATGTTTGAGGAGTGA57630.14691842241786984No Hit
GATTGAAAGCGACAGAGAATAGTTTCGAACAAATAACATTTATGCAAGCC56840.14490444439062505No Hit
TTTGAGGAGTGAGTGAGTGAATAAACTAGCCATGTTCCATGAATTTCTCA56020.14281398618513047No Hit
GGAGTATGCAAGCTGAGGAATTTTAGTTCGTTACAGAAAATGACTTCTAG55630.14181974386788304No Hit
CTATAAAAGGCGAGACAATAAACTTTATCTAGAGACAAAACTAACAATAC51510.1313164660549102No Hit
CTCCAGGGGCTCCCATTACAGTTAGAATAAAACTCAGTGCCCTGTGTCAG50740.1293534748131653No Hit
AGATAGAAGTGGCTCTCAGTTCTAAATTGGTAACAAGGCCGATTTCCACT50460.12863965981616715No Hit
TCCTTTAACTAGTTGATGCCTAGTGGAAATCGGCCTTGTTACCAATTTAG50430.12856317963791736No Hit
GGGCAGAAGTGTGCAGGAGGCAGCGGATCTGGCGGGACAGACCTGAGGAT50220.12802781839016872No Hit
GTGGAGAAGTGGGGAACGCAGTGAACCAGAGAGGGAAGGCACCTCCGCCT49970.12739048357142038No Hit
GTATGCAAGCTGAGGAATTTTAGTTCGTTACAGAAAATGACTTCTAGATA49760.12685512232367177No Hit
CCATATGGCCCACTAAGTTGAAAGTATTTACTATCCGGTCCTTTACAGAA49560.1263452544686731No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG49500.12619229411217348No Hit
GAACCAGAGACTGCAGGATTATCTGTGGCTTTCAATGATCTGTGTTCCGA49120.125223545187676No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTC48430.12346450108793054No Hit
CTGCAGGATTATCTGTGGCTTTCAATGATCTGTGTTCCGAGGGTCTGATG48160.12277617948368233No Hit
TTAGGAGTCTTTCCAAGTTCTTTTTTGTGTGTCCTTTAACTAGTTGATGC47780.12180743055918483No Hit
GACCTGAGGATGACTCGGACGTCACCTCCCTCCTCTGAGCCGCAGTGGCC47760.12175644377368496No Hit
CCCCAAAGCCCTAAGAAGCCATGTGAAATTTAAGAGTAACCATTTAACTA47610.12137404288243596No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC47080.12002289306668945No Hit
ACCAGAGACTGCAGGATTATCTGTGGCTTTCAATGATCTGTGTTCCGAGG46710.11907963753494188No Hit
CCTGTAGATAAGGGGGCAGCAAACCACAGGCTGCAGTCCAAGTCTGGCCC46380.11823835557419404No Hit
GAAATAAGGTGGTTAATTGAAGAAATGCGGCACAGATTGAAAGCGACAGA45900.11701467272219723No Hit
GTATTTACTATCCGGTCCTTTACAGAAGAGGTTGCCAAGTCCTGACACAG45780.11670875200919802No Hit
GTCCTAATCTAATTTATATTTTTAAAATACCCCCCTGGTGGAGGAAAGAC45730.11658128504544836No Hit
CCTCTGAGCCGCAGTGGCCTCATTTCTAAGGCAAAGATGAGGATCAACTC45230.11530661540795165No Hit
ACTACAAGATATGCAAAAACCTTCTGAAATACTCGGGAGTATGCAAGCTG45180.11517914844420198No Hit
GTAGATAAGGGGGCAGCAAACCACAGGCTGCAGTCCAAGTCTGGCCCACC44670.11387898541395534No Hit
GTGTTCCGAGGGTCTGATGTCAGCCATGGAGGATACAGCTGCTGCACCTT44660.1138534920212054No Hit
GAATAGTTTCGAACAAATAACATTTATGCAAGCCTTACAACTACTGCTTG44400.11319066380970713No Hit
CTTCTCCACCTTATAACTTTCCTCAATTTAGGGAGTGGAGTTGATCCTCA44230.11275727613295823No Hit
CTTCTGCCCACTTCTCACCACCTCTCCCGCAGCACCGCATCTCCCTGCAG43960.11206895452871002No Hit
GCCATGCCCACTCCCTTACAAATTGTCTGTGGCTGCTTTTGTGCTACAAC43870.11183951399396062No Hit
GTATTGTTAGTTTTGTCTCTAGATAAAGTTTATTGTCTCGCCTTTTATAG43850.11178852720846076No Hit
CCTCAAGGATGTGGGATGCACTTGACGCCTCTCCCACTGGGCTCTGCCCC43510.11092175185496299No Hit
TTACAGAAGAGGTTGCCAAGTCCTGACACAGGGCACTGAGTTTTATTCTA43340.11048836417821412No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC43080.10982553596671582No Hit
CTTCTGTAAAGGACCGGATAGTAAATACTTTCAACTTAGTGGGCCATATG42750.108984254005968No Hit
GGATACAGCTGCTGCACCTTCTGCAGGCCCCAAAGCCCTAAGAAGCCATG42550.10847438615096933No Hit
CTCCCGAGTATTTCAGAAGGTTTTTGCATATCTTGTAGTTAAATGGTTAC42510.10837241257996959No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT42440.10819395883072006No Hit
GTGCAGGAGGCAGCGGATCTGGCGGGACAGACCTGAGGATGACTCGGACG41540.105899553483226No Hit
GCTATGTAGTATTGTTAGTTTTGTCTCTAGATAAAGTTTATTGTCTCGCC40950.10439544331097989No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT40650.10363064152848185No Hit
GTAAAGGACCGGATAGTAAATACTTTCAACTTAGTGGGCCATATGGTCTC40640.10360514813573193No Hit
GGCTCACACAGAAACCTGTAGATAAGGGGGCAGCAAACCACAGGCTGCAG39810.10148919653748739No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)