FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_F8a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_F8a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335062
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGATCTCTTAAGATGGAAGTGCCAGCAGCTACAGATCCTTCCAAGG65810.49293590859450725No Hit
ATACAGAGGAGACGACTTTTACATTTGTTTTTCTTTTCATTCCTGTTGAA52710.39481312478371794No Hit
GTGGTAAGGGGAGTGAAGGGAAGAGAGAAATTCAGTTTCCTGGAAGATTT50140.37556308246358594No Hit
CCTCTGTATAGATCTCTTAAGATGGAAGTGCCAGCAGCTACAGATCCTTC49070.3675484734042314No Hit
GATCTATACAGAGGAGACGACTTTTACATTTGTTTTTCTTTTCATTCCTG47020.35219338128116895No Hit
GTCTACACACAGAGCACCTGTGACCCATGTTCCTCCCTGCCCCATCCGCA44200.33107076675090746No Hit
GTACTACAGCCTGGGCAACGGAGCGAGACTCTGTCTCAAAAAAACACAAA36330.2721221935760287No Hit
CTGTACTACAGCCTGGGCAACGGAGCGAGACTCTGTCTCAAAAAAACACA33330.24965132705447388No Hit
GTCTTAACATGCGTGCTGTGAGTCCTCCGCAAAGTAGGGACAGTAATACG32480.24328458154003335No Hit
GTCCGAAGACCTACCAGAGAACATGCAGTTCAACAGGAATGAAAAGAAAA31060.23264837138649738No Hit
GGTAAGGGGAGTGAAGGGAAGAGAGAAATTCAGTTTCCTGGAAGATTTGT29590.22163764679093556No Hit
TGTTAAGACACTTTCTTTTGGTCATAGCTGGAGCCCATATTTGAAGCGGA29440.2205141034648578No Hit
ATCCAAGCTATCCCCTCAGTGAAACAAATGCTTGAGAAAGAAGATTTTTA28060.21017750486494258No Hit
GTGCCAGGCAGTGCTCTAAGTGGATGTCACTGAAGTCCTGTAGCCAGGAG26480.19834284849692374No Hit
GAACATAGATTTAAATTCAGAAGCTGGGAGTATATCCTGAGGACAGACAC26090.19542163584912162No Hit
GCTCTAAGTGGATGTCACTGAAGTCCTGTAGCCAGGAGCTATTCGTATTA25880.19384867519261278No Hit
GTCCAGACACCTGAGTCCAGTCCCTGGGGACTTCAGGCTGGGTTATTTTG25490.19092746254481066No Hit
GCCTTTTATGGGAGGGATTTTTTTTAAAAACAAACTCCACCAGCTCAGGT25460.19070275387959512No Hit
TGTCTGGACTGAGGAAAATCCCTCTGGTACCCACTGTCCTTTCCTCTATG25440.19055294810278472No Hit
GTAATACGAATAGCTCCTGGCTACAGGACTTCAGTGACATCCACTTAGAG25200.18875527878106035No Hit
TCCCTGCCCCATCCGCAGGGGTGCCTGCTCCTCCAAGATTAGACTAAGGA24200.1812649899405421No Hit
CCCTAAACCAGCTCATCCGCTTCAAATATGGGCTCCAGCTATGACCAAAA23920.17916770906519697No Hit
GCCTGGCACCTTGGAAGGATCTGTAGCTGCTGGCACTTCCATCTTAAGAG23910.1790928061767918No Hit
GGTTTAGGGGCCGTACTCAGCTGGTTGAGTGTCATAGAGGAAAGGACAGT23800.17826887440433478No Hit
TCGTATTACTGTCCCTACTTTGCGGAGGACTCACAGCACGCATGTTAAGA23700.17751984552028297No Hit
ACTTAGAGCACTGCCTGGCACCTTGGAAGGATCTGTAGCTGCTGGCACTT23320.174673535760886No Hit
GAATATGGGAGGCGAAGGTTGCAGTGAGCCAAGATCGCACCACTGTACTA22340.1673330526971781No Hit
GAGTATATCCTGAGGACAGACACAAAGAAACCCCATGGTCCGAAGACCTA21740.16283887939286715No Hit
GGATAGTGGTAAGGGGAGTGAAGGGAAGAGAGAAATTCAGTTTCCTGGAA21640.1620898505088153No Hit
GTCCTTTCCTCTATGACACTCAACCAGCTGAGTACGGCCCCTAAACCAGC21610.16186514184359976No Hit
CCTGTGACCCATGTTCCTCCCTGCCCCATCCGCAGGGGTGCCTGCTCCTC21450.16066669562911684No Hit
GAGTAGATGAAGTCTACACACAGAGCACCTGTGACCCATGTTCCTCCCTG21300.15954315230303912No Hit
GTCTCAACCTGAGCTGGTGGAGTTTGTTTTTAAAAAAAATCCCTCCCATA19190.14373864284954557No Hit
GGTACCCACTGTCCTTTCCTCTATGACACTCAACCAGCTGAGTACGGCCC19110.14313941974230412No Hit
GCTGAGTACGGCCCCTAAACCAGCTCATCCGCTTCAAATATGGGCTCCAG19020.14246529374665745No Hit
GCTCTGTGATGAGCCCTGAGATGTTTGAAGTGAGAACTCTCCAAACAGCC18790.14074252731333825No Hit
GGTTGAGACAGGCAAATTTCTTTCTTATCTTTCTATGCCTAAAAATCTTC18770.1405927215365279No Hit
GCCCTGAGATGTTTGAAGTGAGAACTCTCCAAACAGCCTCCGTGGGTATA18430.13804602333075167No Hit
GTATTACTGTCCCTACTTTGCGGAGGACTCACAGCACGCATGTTAAGACA18290.1369973828930791No Hit
AGCTTGGATAGCCTCATTTTAGTTGATGTCACCATTTCAATCCCTACTTT18180.1361734511206221No Hit
CCCTAGCTCCCTCATGCTCCACGACTTGGGGACAAGCTAGTTGTCTTCTC17940.13437578179889773No Hit
CAGTAATACGAATAGCTCCTGGCTACAGGACTTCAGTGACATCCACTTAG17860.13377655869165628No Hit
ACTACAGCCTGGGCAACGGAGCGAGACTCTGTCTCAAAAAAACACAAACA17690.13250320958876816No Hit
ATAAAAGGCTGGGCAGAGGGGCGTTACACCTGAGGGAATTTTCCAGATTA17690.13250320958876816No Hit
AGATTAGACTAAGGACAAAATAACCCAGCCTGAAGTCCCCAGGGACTGGA17400.13033102582501788No Hit
GCATGGAACATAGATTTAAATTCAGAAGCTGGGAGTATATCCTGAGGACA17370.1301063171598023No Hit
ATTCAGGAGACTGCATGGAACATAGATTTAAATTCAGAAGCTGGGAGTAT17350.12995651138299197No Hit
GAGCACCTGTGACCCATGTTCCTCCCTGCCCCATCCGCAGGGGTGCCTGC17310.1296568998293712No Hit
AAATAACCCAGCCTGAAGTCCCCAGGGACTGGACTCAGGTGTCTGGACTG17250.12920748249894012No Hit
GGAAAGGACAGTGGGTACCAGAGGGATTTTCCTCAGTCCAGACACCTGAG16960.12703529873518984No Hit
GTCTTACTCTGTCACCCAGGCTGGAGTGCAGTGGCATGATCTTGGCTCAC16800.1258368525207069No Hit
GTTCAACAGGAATGAAAAGAAAAACAAATGTAAAAGTCGTCTCCTCTGTA16630.1245635034178188No Hit
CTACAGATCCTTCCAAGGTGCCAGGCAGTGCTCTAAGTGGATGTCACTGA16090.12051874744393894No Hit
GTTCGTTGAGATGGCATTTCCATGGAATTCAGGAGACTGCATGGAACATA15980.11969481567148192No Hit
TCTCAGGGCTCATCACAGAGCTTTCCATGCATGTTCCCAGGCAAGTCTCT15960.11954500989467157No Hit
TCACAGAGCTTTCCATGCATGTTCCCAGGCAAGTCTCTTGTGGTCAGGGA15820.11849636945699901No Hit
GTAGATGAAGTCTACACACAGAGCACCTGTGACCCATGTTCCTCCCTGCC15610.11692340880049017No Hit
GTGGGTACCAGAGGGATTTTCCTCAGTCCAGACACCTGAGTCCAGTCCCT15580.11669870013527463No Hit
CCTGAGGACAGACACAAAGAAACCCCATGGTCCGAAGACCTACCAGAGAA15430.11557515680919689No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT15030.11257904127298957No Hit
AGTCTACACACAGAGCACCTGTGACCCATGTTCCTCCCTGCCCCATCCGC14810.11093117772807556No Hit
CTTAAGATGGAAGTGCCAGCAGCTACAGATCCTTCCAAGGTGCCAGGCAG14780.11070646906286No Hit
CTGTATAGATCTCTTAAGATGGAAGTGCCAGCAGCTACAGATCCTTCCAA14640.10965782862518744No Hit
CTTCCAAGGTGCCAGGCAGTGCTCTAAGTGGATGTCACTGAAGTCCTGTA14550.10898370262954081No Hit
TCTATACAGAGGAGACGACTTTTACATTTGTTTTTCTTTTCATTCCTGTT14420.10800996508027344No Hit
GTCTTCGGACCATGGGGTTTCTTTGTGTCTGTCCTCAGGATATACTCCCA14070.10538836398609204No Hit
GTCCCTACTTTGCGGAGGACTCACAGCACGCATGTTAAGACACTTTCTTT13770.10314127733393655No Hit
CCTTGGAAGGATCTGTAGCTGCTGGCACTTCCATCTTAAGAGATCTATAC13660.10231734556147955No Hit
CTTCAAATATGGGCTCCAGCTATGACCAAAAGAAAGTGTCTTAACATGCG13550.10149341378902253No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA13530.10134360801221218No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)