FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_F8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_F8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1210718
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA58690.4847536750919702No Hit
GGATACACCATGCAATCTATTCGAGAAATTTTTCCCTAGCAGTTCATATA45660.37713158638097394No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT34410.2842115174631913No Hit
GTTCATTGATGCTTAATGCATGTCCATTATTCTCTTGTCTGCTGTAATTG32900.27173957932400444No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG32870.27149179247355704No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG30220.2496039540173682No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCATGGTGTAT30080.2484476153819469No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG28760.23754499396226042No Hit
CATAAATACGAGGCTTTTCATACGTAAAGTAAACGTCTTTATCGACGATC28430.23481933860733878No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA28080.23192849201878557No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT27850.230028792832022No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA26360.21772204592646677No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG25700.2122707352166235No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA25450.21020584479622836No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC24970.20624125518906963No Hit
ATAAAGAAGGGATCGTCGATAAAGACGTTTACTTTACGTATGAAAAGCCT24460.20202887873146347No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC23630.19517344253575153No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA22610.18674868962053923No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG22600.18666609400372342No Hit
GTATTAATGTAAACGTTTCTGTACGCTTATATGCAAACAACGACAACGTG22490.18575754221874954No Hit
GCTTAATGCATGTCCATTATTCTCTTGTCTGCTGTAATTGGGTATCTCAT21320.17609385505130012No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG20960.1731204128459311No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG20500.16932101447240397No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG19550.16147443087490232No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT19150.15817060620227005No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG18820.15544495084734844No Hit
CTATAAAGAAGGGATCGTCGATAAAGACGTTTACTTTACGTATGAAAAGC18620.1537930385110323No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG17580.1452030943621884No Hit
GTATTTATGTGTGTAAATGAACGAGCGTACGTAGTTATCTCTGTTTTCTT17360.14338599079224063No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG17230.14231224777363516No Hit
TTGCAAGATTGCTCAGTTCATTGATGCTTAATGCATGTCCATTATTCTCT16350.13504383349384416No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC16040.13248336937255412No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG15850.1309140526530538No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG15750.13008809648489575No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT15580.12868397099902704No Hit
CCCATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTA15360.1268668674290793No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGCTAATGTGCTAATTGGGCAA15340.12670167619544767No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC15290.12628869811136864No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC15260.1260409112609212No Hit
GAGTAAGACCTGCTGTTGCATCTTCGCCATTGTTTGCTTGGCGCCAAACT15070.12447159454142087No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA14860.12273708658828893No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT14830.1224892997378415No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG14570.12034181370063053No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGCAATCTATTCGAGA14560.12025921808381473No Hit
GTGGAGGCCATGGTGTCTAGGGCCCGGATTGATGCCAGGGTCGACTTCGA14440.11926807068202505No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT14430.11918547506520924No Hit
CTTATATGCAAACAACGACAACGTGTATCTCTGCGTTGAATACTCTTGCG14240.1176161583457089No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA14210.1173683714952615No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT14100.11645981971028761No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC14040.11596424600939279No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC14040.11596424600939279No Hit
GGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAG13900.11480790737397148No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCATGGTGT13800.11398195120581339No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG13760.11365156873855019No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT13550.11191706078541824No Hit
GCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCT13530.11175186955178662No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC13500.11150408270133921No Hit
ATCCAGAATAGCATAACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGA13240.10935659666412824No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA13190.1089436185800492No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGCAATCTATTCG13120.10836544926233856No Hit
GTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAGG13100.10820025802870693No Hit
CCATTATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCAC13080.10803506679507532No Hit
GTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTACGGTCTTGGGA13070.10795247117825953No Hit
CATATAGACTGTTGAATACAGATTAAACTTCCAAATGTGTGCATATTGCA13050.1077872799446279No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG12960.10704391939328564No Hit
ATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGA12830.10597017637468013No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA12720.10506162458970628No Hit
ATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGACA12630.10431826403836401No Hit
GTATTCAACAGTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGA12580.10390528595428498No Hit
GGAAAGGAAGAGAGGAAGAAAAACCTAAAGAAGGAGAGAAAGGAAGAAAG12490.10316192540294272No Hit
TCTATACACAGGTTGGATAGAAGAGCCTTTGTGGTCTAGAAAGGTTAGAT12450.10283154293567948No Hit
CACCTACATCTATCATACAGAAGTCTATTTCCATACAACCGATACGTATT12320.10175779991707401No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT12320.10175779991707401No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT12230.10101443936573173No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)