FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_F7a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_F7a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences652840
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC46630.714263831873047No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT41760.6396666870902518No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT40260.6166901537895962No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA38560.5906500827155199No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA22860.3501623675019913No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT22110.3386741008516635No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC21420.328104895533362No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG20720.31738251332638934No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT20410.31263402977758714No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC19890.3046688315666932No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC18640.28552172048281355No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT18630.28536854359414254No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA18170.2783224067152748No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT18140.2778628760492617No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAAT16960.2597880031860793No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCAAGCG16850.2581030574106979No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT16330.2501378591998039No Hit
GGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA16090.24646161387169904No Hit
GTTAAAGATTACACTGAAGTTCGCTTTCAGTACTATGTTCTTTTCCATGA16050.24584890631701486No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTC15630.23941547699283133No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCA15000.22976533300655597No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA14950.22899944856320079No Hit
ATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA14800.22670179523313522No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT14550.2228723730163593No Hit
GTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATGAGCA14520.22241284235034617No Hit
CGATTGGACCAGGCGATCATGGAAAAGAACATAGTACTGAAAGCGAACTT14410.22072789657496478No Hit
TCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTT14340.21965565835426754No Hit
GTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTTTCAAG14280.2187365970222413No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC14170.21705165124685988No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTT13800.2113841063660315No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG13710.21000551436799217No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT13690.20969916059065005No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG13450.2060229152625452No Hit
ATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG13430.2057165614852031No Hit
ACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACC13400.20525703081919No Hit
TGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAA13370.20479750015317688No Hit
CATCTGTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAGGAAATG13260.2031125543777955No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT13020.1994363090496906No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC12980.1988236014950064No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC12830.19652594816494087No Hit
GTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAGGAAATGTCACG12820.19637277127626984No Hit
GCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGAAAGGTAG12480.19116475706145455No Hit
TTTCATTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTT12470.19101158017278352No Hit
CCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTC12410.1900925188407573No Hit
TTCTAAGTGTCTCGCTGGATTCCTCTTTCAAGATCCATTCCACGATTTGT12390.18978616506341522No Hit
CTCCCATTCTCATCACAGTTTCTCCAAGCGAATCTCTGTATATTTTCAGA12240.18748851173334966No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT12210.18702898106733656No Hit
AGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGATCATGGAAAAG11990.18365908951657375No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTT11810.18090190552049507No Hit
GTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGAT11750.17998284418846883No Hit
CTCCAGAGCAGAAATGAAAAGTGGCGAGAGCAATTGGGACAGAAATTTGA11680.17891060596777159No Hit
GTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTCCTCCG11280.1727835304209301No Hit
GTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGTACTATGTT11120.1703327002021935No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG11100.17002634642485143No Hit
TCTCTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTC10950.16772869309478586No Hit
GAATGGGAGACCTTCACTACCTCCAGAGCAGAAATGAAAAGTGGCGAGAG10950.16772869309478586No Hit
TGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCG10920.16726916242877274No Hit
CTTCCAGGACATACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCAT10730.16435880154402305No Hit
CAATGGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAA10590.1622143251026285No Hit
GTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACCTTGAT10540.16144844065927333No Hit
AGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTC10540.16144844065927333No Hit
CTATTGCTCCCTCCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAAT10530.1612952637706023No Hit
CCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATT10490.16068255621591815No Hit
CTCTTGTTGGGAAACAAATCGTGGAATGGATCTTGAAAGAGGAATCCAGC10490.16068255621591815No Hit
GCCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTT10470.16037620243857606No Hit
GTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAATTTCTGTCCCA10420.15961031799522088No Hit
CAGGTAGACTGTTTCCTTTGGCATATCCGCAAGCGATTTGCAGACAATGG10370.1588444335518657No Hit
GTGTAATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACT10290.1576190184424974No Hit
CACTTAGAATGACAATTGCATCTGTACCTACTTCGCGCTACCTTTCTGAC10250.15700631088781325No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG10110.15486183444641874No Hit
GAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGA10050.1539427731143925No Hit
CTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTCCTC10040.15378959622572147No Hit
TTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTTGAC10010.15333006555970835No Hit
ATACAGAGATTCGCTTGGAGAAACTGTGATGAGAATGGGAGACCTTCACT9870.1511855891183138No Hit
GTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTA9850.15087923534097175No Hit
GAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGGACATACT9830.1505728815636297No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTC9820.15041970467495866No Hit
GTAGGTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTTT9670.1481220513448931No Hit
TTCAAGATCCATTCCACGATTTGTTTCCCAACAAGAGTGGCTGTTTCGAT9670.1481220513448931No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC9660.14796887445622206No Hit
GCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA9660.14796887445622206No Hit
GACTCGAACCGTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAA9600.14704981312419582No Hit
GAATGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGA9570.1465902824581827No Hit
ACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGGCC9570.1465902824581827No Hit
CACTAGACCAAAAGCAGCTTCTGTGGTCACTGTTCCCATCCTGTTGTATA9530.14597757490349858No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT9520.14582439801482752No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC9350.1432203909074199No Hit
GCCTAGGCATGAGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATG9280.14214815268672262No Hit
CTATTATCTTTTGCCTAGGCATGAGCATGAACCAGTCTCGTGACATTTCC9240.1415354451320385No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTTGAAAATTTGCAGG9190.1407695606886833No Hit
AGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGAAAGGTA9190.1407695606886833No Hit
CTCCAGTGCTGGTCTGAAAGATGACCTTCTTGAAAATTTGCAGGCCTACC9190.1407695606886833No Hit
GAAATAAGGTGGTTAATTGAAGAAATGCGGCACAGATTGAAAGCGACAGA9170.1404632069113412No Hit
GAAAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATT9170.1404632069113412No Hit
ATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTA9110.139544145579315No Hit
GATTGAAAGCGACAGAGAATAGTTTCGAACAAATAACATTTATGCAAGCC9030.1383187304699467No Hit
ACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGA8880.13602107713988112No Hit
TAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTT8800.13479566203051285No Hit
GTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAG8780.13448930825317076No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC8760.13418295447582868No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC8720.13357024692114453No Hit
ACACTGAAGTTCGCTTTCAGTACTATGTTCTTTTCCATGATCGCCTGGTC8710.1334170700324735No Hit
ATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTTGACATCCTCATAA8690.13311071625513143No Hit
ACCTTTCTGACATGACCCTCGAGGAAATGTCACGAGACTGGTTCATGCTC8620.13203847803443416No Hit
CATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATT8610.13188530114576313No Hit
GGACATACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGG8520.1305067091477238No Hit
GTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAAACTGTGATGA8520.1305067091477238No Hit
GTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACT8460.12958764781569757No Hit
GAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG8460.12958764781569757No Hit
AAGTAGGTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCT8390.1285154095950003No Hit
GCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGATTGGACC8320.12744317137430305No Hit
GTCATGGGCTGGGCAGTGAGGATCAGGGGCAAGGCCATACCGCCGCATGC8180.12529869493290852No Hit
GGCATGAGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGA8150.1248391642668954No Hit
CTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAAT8140.12468598737822437No Hit
TCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTCCCTCCT8130.12453281048955334No Hit
CTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTA8080.12376692604619814No Hit
GGCCAAGGGTGTTGCCTCTTCCTTTTAAGGACTTTTGATCTCGGCGGAGC7990.12238833404815881No Hit
GGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAAA7980.12223515715948778No Hit
AGATTACACTGAAGTTCGCTTTCAGTACTATGTTCTTTTCCATGATCGCC7920.12131609582746156No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAC7900.1210097420501195No Hit
CCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCG7890.12085656516144844No Hit
GCACTGGAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTG7860.12039703449543532No Hit
ATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGACAAAGC7840.12009068071809326No Hit
GGCAACACCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACA7790.11932479627473806No Hit
GATCTTGAAAGAGGAATCCAGCGAGACACTTAGAATGACAATTGCATCTG7770.11901844249739599No Hit
ATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGT7750.11871208872005393No Hit
ATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTC7750.11871208872005393No Hit
GGCAAAAGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGATCATG7740.1185589118313829No Hit
CAATTGCATCTGTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAG7730.11840573494271184No Hit
AGACTGGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCG7680.11763985049935667No Hit
CTCTTTGCGTGCGATTGGACCAGGCGATCATGGAAAAGAACATAGTACTG7610.11656761227865939No Hit
GAAATGAAAAGTGGCGAGAGCAATTGGGACAGAAATTTGAGGAAATAAGG7570.11595490472397524No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC7530.1153421971692911No Hit
GTAGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCAAGCGAATCTCTGT7460.11426995894859385No Hit
GATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGATCATGGAAAAGA7460.11426995894859385No Hit
GTTCACAAGTGGCACACACTAGACCAAAAGCAGCTTCTGTGGTCACTGTT7420.11365725139390967No Hit
GTTCCCATCCTGTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGT7330.11227865939587035No Hit
TACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTG7310.11197230561852828No Hit
CCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTTGACATCCTCAT7300.11181912872985725No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAGATGGCT7250.11105324428650205No Hit
AATATACAGAGATTCGCTTGGAGAAACTGTGATGAGAATGGGAGACCTTC7250.11105324428650205No Hit
TCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTCTACTTCAA7180.1099810060658048No Hit
CCTTAAAAGGAAGAGGCAACACCCTTGGCCTCGATATCGAAACAGCCACT7120.10906194473377856No Hit
GCATGTACCATCTGCCTAGTCTGATTAGCAACCTCCATGGCCTCCGCTGC7110.10890876784510753No Hit
AAAGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGATCATGGAAA7040.10783652962441027No Hit
GAAAATATACAGAGATTCGCTTGGAGAAACTGTGATGAGAATGGGAGACC7040.10783652962441027No Hit
ATACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTT6940.10630476073769989No Hit
TAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGC6940.10630476073769989No Hit
GATCAAAAGTCCTTAAAAGGAAGAGGCAACACCCTTGGCCTCGATATCGA6850.10492616873966057No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACG6790.10400710740763434No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC6780.10385393051896329No Hit
AAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCTTTC6780.10385393051896329No Hit
CTGCTGTTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG6770.10370075363029226No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT6770.10370075363029226No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG6770.10370075363029226No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA6750.10339439985295018No Hit
CTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGT6730.10308804607560812No Hit
GTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGC6690.10247533852092397No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGAC6670.1021689847435819No Hit
ATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCCGCAAGCGATT6650.1018626309662398No Hit
GTCTGTGAGACCGATGCTGTGAATCAGCAATCTGTTCACAAGTGGCACAC6630.10155627718889775No Hit
GTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTCCCTCC6530.10002450830218737No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)