FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_F6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_F6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7110248
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT204120.2870785941643667No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT170060.23917590497546642No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT161670.22737603526628045No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA160830.22619464187465754No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT145920.20522490917335093No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA143700.20210265520977608No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC140550.19767242999119017No Hit
GATTGAAAGCGACAGAGAATAGTTTCGAACAAATAACATTTATGCAAGCC101710.14304704983567382No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTC101310.1424844815539486No Hit
GAAATAAGGTGGTTAATTGAAGAAATGCGGCACAGATTGAAAGCGACAGA100160.14086709774398867No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG98710.13882778772273485No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG95520.13434130567597644No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG88460.12441197550352676No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT88200.12404630612040536No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACG87890.12361031570206835No Hit
GTATAAGGGAATGTGGTGCTTATGGCATTTTGCGCTGGAATTTTTAGGAA83760.11780179819325572No Hit
GATCAAGAAAGAAGAGTTCTCTGAGATCATGAAGATCTGTTCCACCATTG83560.11752051405239311No Hit
TCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTCTACTTCAA83070.11683136790727974No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTC82700.11631099224668393No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC78670.11064311680830262No Hit
GAATAGTTTCGAACAAATAACATTTATGCAAGCCTTACAACTACTGCTTG78150.10991177804205986No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC77550.10906792561947207No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA77320.10874444885748008No Hit
CCTTACAACTACTGCTTGAAGTAGAACAAGAGATAAGAGCTTTCTCGTTT77230.10861787099409191No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT76790.10799904588419419No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC76600.10773182595037473No Hit
AGTCTGGACGGATCAAGAAAGAAGAGTTCTCTGAGATCATGAAGATCTGT76010.10690203773483006No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG75640.10638166207423427No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT75130.10566438751503464No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC73890.10392042584168654No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT72330.10172640954295828No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAAT71870.1010794560189743No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC71810.10099507077671553No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)