FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_F3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_F3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4317383
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT128090.29668435716729324No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT106980.24778899625073797No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA101890.23599944688715366No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC84990.19685536353851396No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT81030.1876831404580043No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT78950.18286540712278712No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC75590.17508291481205165No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT74960.17362369750378875No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA68770.15928630839561836No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA65110.1508089506999958No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG64140.14856221928886088No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT62590.1449720814669442No Hit
ACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACC62410.14455516223601195No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA60800.1408260513371179No Hit
GATTGAAAGCGACAGAGAATAGTTTCGAACAAATAACATTTATGCAAGCC59480.13776864364361466No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG57720.13369210005227705No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA57640.13350680261630715No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACG57590.13339099171882596No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC55080.1275772846652706No Hit
CTTCTAGGTATTTATTTCTTCTCTCATCAAAAGCAGAAAGCACCATCCTC52360.12127717184229428No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC51630.11958633273906902No Hit
GAAATAAGGTGGTTAATTGAAGAAATGCGGCACAGATTGAAAGCGACAGA51590.11949368402108407No Hit
GAAAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATT50960.11803446671282117No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC50210.11629730325060345No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT50020.11585722184017494No Hit
GTAATGGACAGGCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATA50010.11583405966067871No Hit
ATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA49770.11527816735276902No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA49240.11405057183946848No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC48220.11168802953085237No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT47170.10925600068374755No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG47070.10902437888878518No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT46760.10830635132440185No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTC46740.10826002696540937No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT46470.10763464811901098No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT46360.10737986414455238No Hit
GAATAGTTTCGAACAAATAACATTTATGCAAGCCTTACAACTACTGCTTG46220.10705559363160506No Hit
GTCCATTACTTGGTCCATCGGTCATTACAGTAAAGCAAGAACCATTTACA45750.10596697119528195No Hit
GAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGA45660.10575851157981583No Hit
ATCCAGAATAGCATAACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGA45620.1056658628618309No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA45430.10522578145140239No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC45130.1045309160665153No Hit
CTTCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGG44490.10304853657875615No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)