FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_F10a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_F10a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2376747
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTATTTAGTCTACTGATTCTAATCCCAATCTCTTCAGGAAAACTCTC567462.3875490323538853No Hit
GGGTTAAGGGATGCCCAAATAGTTGATGAAACATGATTTATGTTTCTCTG276701.164196273309696No Hit
TTCTAATCCCAATCTCTTCAGGAAAACTCTCAGAGAAACATAAATCATGT268291.128811775085863No Hit
GGTTAAGGGATGCCCAAATAGTTGATGAAACATGATTTATGTTTCTCTGA224820.9459147313534002No Hit
CCCAAATAGTTGATGAAACATGATTTATGTTTCTCTGAGAGTTTTCCTGA210380.8851594216801367No Hit
GTTAAGGGATGCCCAAATAGTTGATGAAACATGATTTATGTTTCTCTGAG171860.7230891634658632No Hit
GGTAGAAAGTGAGTGATTCATCACCAAGGTCATGCTGTCTGTCATGTTGT143980.6057859755371523No Hit
GTTCTATTCAGGCCTTCAAGGAATTGGATAATGTCCACCCACATTGTGGA136900.5759973610990147No Hit
GATTTATGTTTCTCTGAGAGTTTTCCTGAAGAGATTGGGATTAGAATCAG130550.5492801715958829No Hit
GTCTGTCATGTTGTGACTTTTATGCTGGGTTTTACTGAATCAACAATGGA120380.506490594076694No Hit
ACAATGGAGTGTAATGGTTAACTTTGTGTGCCAACTTGACTGGGTTAAGG114570.4820454175391828No Hit
GTGTAATGGTTAACTTTGTGTGCCAACTTGACTGGGTTAAGGGATGCCCA114460.48158260008322296No Hit
GTTTTACTGAATCAACAATGGAGTGTAATGGTTAACTTTGTGTGCCAACT113470.4774172429795851No Hit
TGATTTATGTTTCTCTGAGAGTTTTCCTGAAGAGATTGGGATTAGAATCA108710.4573898694307808No Hit
AAATAAAGAAGACCCAGAGTCCACAATGTGGGTGGACATTATCCAATTCC108420.4561697143196142No Hit
CTTCAGGAAAACTCTCAGAGAAACATAAATCATGTTTCATCAACTATTTG105970.4458615073459649No Hit
GTTTTGTTCTATTCAGGCCTTCAAGGAATTGGATAATGTCCACCCACATT103980.43748871882451096No Hit
CTTTTATGCTGGGTTTTACTGAATCAACAATGGAGTGTAATGGTTAACTT101370.4265073228240111No Hit
GGCCCAGGCTCATCAGCAGCTGGCCAAAGCCCTTCCCAAGCTGCCTTCTG98690.41523140662426417No Hit
TTCCTGAAGAGATTGGGATTAGAATCAGTAGACTAAATAAAGAAGACCCA94670.3983175323246437No Hit
GTCCTACCTTTACTGTTTTGTTCTATTCAGGCCTTCAAGGAATTGGATAA91870.38653672435475883No Hit
CAACAATGGAGTGTAATGGTTAACTTTGTGTGCCAACTTGACTGGGTTAA90360.38018350291385666No Hit
GAATATCCCAGCTCGGAGACAAAGTGTGTCCTACCTTTACTGTTTTGTTC89040.3746296934423395No Hit
ACTGATTCTAATCCCAATCTCTTCAGGAAAACTCTCAGAGAAACATAAAT87380.36764535728876485No Hit
GTCATGTTGTGACTTTTATGCTGGGTTTTACTGAATCAACAATGGAGTGT85760.36082931839190285No Hit
TTACTGTTTTGTTCTATTCAGGCCTTCAAGGAATTGGATAATGTCCACCC81710.3437892211497479No Hit
ATTCAGGCCTTCAAGGAATTGGATAATGTCCACCCACATTGTGGACTCTG79770.3356268041991849No Hit
GAATTGGATAATGTCCACCCACATTGTGGACTCTGGGTCTTCTTTATTTA78830.33167181866643775No Hit
ACCTTTACTGTTTTGTTCTATTCAGGCCTTCAAGGAATTGGATAATGTCC78140.3287686909881447No Hit
CCCTTAACCCAGTCAAGTTGGCACACAAAGTTAACCATTACACTCCATTG76680.3226258411181333No Hit
CTGTCATGTTGTGACTTTTATGCTGGGTTTTACTGAATCAACAATGGAGT75180.31631469399140927No Hit
GGATTAGAATCAGTAGACTAAATAAAGAAGACCCAGAGTCCACAATGTGG74480.31336949199893804No Hit
AAGTTAACCATTACACTCCATTGTTGATTCAGTAAAACCCAGCATAAAAG72690.3058381897610474No Hit
GTTGATGAAACATGATTTATGTTTCTCTGAGAGTTTTCCTGAAGAGATTG71250.2997794885193923No Hit
AGTCTACTGATTCTAATCCCAATCTCTTCAGGAAAACTCTCAGAGAAACA71120.29923252243507614No Hit
CATTAACACATGTAACATCTCCATTTCAATGATGAACAGACATATCAAAT70670.29733917829705897No Hit
GTTGCAGAGGGTCTAGTTGGGAGGGAAGGAGCTGGACATGGTGGGTGGGA70650.29725502966870265No Hit
GGTTAACTTTGTGTGCCAACTTGACTGGGTTAAGGGATGCCCAAATAGTT70600.29704465809781183No Hit
GGATAATGTCCACCCACATTGTGGACTCTGGGTCTTCTTTATTTAGTCTA70130.29506716533143834No Hit
ATTCATCACCAAGGTCATGCTGTCTGTCATGTTGTGACTTTTATGCTGGG68870.2897658017449901No Hit
CCTTTACTGTTTTGTTCTATTCAGGCCTTCAAGGAATTGGATAATGTCCA68000.28610533641149016No Hit
CATTGTGGACTCTGGGTCTTCTTTATTTAGTCTACTGATTCTAATCCCAA65760.27668069003558227No Hit
ATGTTTCTCTGAGAGTTTTCCTGAAGAGATTGGGATTAGAATCAGTAGAC65200.27432452844160526No Hit
ATTTAGTCTACTGATTCTAATCCCAATCTCTTCAGGAAAACTCTCAGAGA64980.27339889352968577No Hit
GTCTACTGATTCTAATCCCAATCTCTTCAGGAAAACTCTCAGAGAAACAT64810.27268363018865704No Hit
GGGTTTTACTGAATCAACAATGGAGTGTAATGGTTAACTTTGTGTGCCAA62840.26439499029555946No Hit
GTCATGCTGTCTGTCATGTTGTGACTTTTATGCTGGGTTTTACTGAATCA62200.2617022341881572No Hit
ATCAACAATGGAGTGTAATGGTTAACTTTGTGTGCCAACTTGACTGGGTT61610.25921984965164574No Hit
ACATTGTGGACTCTGGGTCTTCTTTATTTAGTCTACTGATTCTAATCCCA60170.25316114840999066No Hit
GAGCTGGACATGGTGGGTGGGAAGAGGTGGGTGGCAGAAGGCAGCTTGGG59950.2522355134980711No Hit
CATCAGCAGCTGGCCAAAGCCCTTCCCAAGCTGCCTTCTGCCACCCACCT59670.2510574327010826No Hit
GAGACAAAGTGTGTCCTACCTTTACTGTTTTGTTCTATTCAGGCCTTCAA57270.24095959729832414No Hit
GACAAAGTGTGTCCTACCTTTACTGTTTTGTTCTATTCAGGCCTTCAAGG56980.23973944218715748No Hit
ATTCTAATCCCAATCTCTTCAGGAAAACTCTCAGAGAAACATAAATCATG55040.23157702523659437No Hit
GGCAAAGGCCACTGGTGGGGGTTTGCAGGGATCAGTTGCAGAGGGTCTAG54380.22880012050083579No Hit
GTTTTGAGGGATGATCAAAATTTGTTTTTAGACATGCTAGATTTGATATG53070.2232883853434968No Hit
GTAATGGTTAACTTTGTGTGCCAACTTGACTGGGTTAAGGGATGCCCAAA51830.21807117038540494No Hit
AAACATGATTTATGTTTCTCTGAGAGTTTTCCTGAAGAGATTGGGATTAG50680.21323262425491646No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT49970.21024534794826713No Hit
CTATTTGGGCATCCCTTAACCCAGTCAAGTTGGCACACAAAGTTAACCAT49220.20708977438490508No Hit
ACATAAATCATGTTTCATCAACTATTTGGGCATCCCTTAACCCAGTCAAG48990.20612206515880738No Hit
CTACTGATTCTAATCCCAATCTCTTCAGGAAAACTCTCAGAGAAACATAA48880.20565924770284766No Hit
CTCTGGGTCTTCTTTATTTAGTCTACTGATTCTAATCCCAATCTCTTCAG48600.20448116690585916No Hit
ATTTATGTTTCTCTGAGAGTTTTCCTGAAGAGATTGGGATTAGAATCAGT48490.20401834944989938No Hit
GCCCAAATAGTTGATGAAACATGATTTATGTTTCTCTGAGAGTTTTCCTG48340.20338723473722697No Hit
TCACCAAGGTCATGCTGTCTGTCATGTTGTGACTTTTATGCTGGGTTTTA47540.20002128960297416No Hit
ATCCCAATCTCTTCAGGAAAACTCTCAGAGAAACATAAATCATGTTTCAT46520.1957297095568018No Hit
TCCCTTAACCCAGTCAAGTTGGCACACAAAGTTAACCATTACACTCCATT45880.19303695344939953No Hit
AGGTCATGCTGTCTGTCATGTTGTGACTTTTATGCTGGGTTTTACTGAAT45850.19291073050686505No Hit
ATTGTGGACTCTGGGTCTTCTTTATTTAGTCTACTGATTCTAATCCCAAT45450.19122775793973865No Hit
TTACATATGTATGTGTACACATTAACACATGTAACATCTCCATTTCAATG45040.18950271105843405No Hit
GAGATGAATATCCCAGCTCGGAGACAAAGTGTGTCCTACCTTTACTGTTT44730.1881984073189111No Hit
TAGTTGATGAAACATGATTTATGTTTCTCTGAGAGTTTTCCTGAAGAGAT44140.18571602278239965No Hit
AGCGAAAGCAGGGGCCCAGGCTCATCAGCAGCTGGCCAAAGCCCTTCCCA43850.184495867671233No Hit
GTCTAGTTGGGAGGGAAGGAGCTGGACATGGTGGGTGGGAAGAGGTGGGT43520.1831074153033537No Hit
ACATATGTATGTGTACACATTAACACATGTAACATCTCCATTTCAATGAT43390.1825604492190376No Hit
CATTACATATGTATGTGTACACATTAACACATGTAACATCTCCATTTCAA43220.18184518587800888No Hit
CTCTGAGAGTTTTCCTGAAGAGATTGGGATTAGAATCAGTAGACTAAATA42630.17936280134149743No Hit
CTGGTGGGGGTTTGCAGGGATCAGTTGCAGAGGGTCTAGTTGGGAGGGAA40460.17023267516483664No Hit
GGCCACTGGTGGGGGTTTGCAGGGATCAGTTGCAGAGGGTCTAGTTGGGA39300.16535205472017006No Hit
GGAGTGTAATGGTTAACTTTGTGTGCCAACTTGACTGGGTTAAGGGATGC38660.1626592986127678No Hit
AACATGATTTATGTTTCTCTGAGAGTTTTCCTGAAGAGATTGGGATTAGA37870.1593354277926931No Hit
CTCTTCAGGAAAACTCTCAGAGAAACATAAATCATGTTTCATCAACTATT37750.1588305360225552No Hit
CCATTACACTCCATTGTTGATTCAGTAAAACCCAGCATAAAAGTCACAAC37240.15668474599946902No Hit
TGCCCAAATAGTTGATGAAACATGATTTATGTTTCTCTGAGAGTTTTCCT37190.15647437442857823No Hit
TTATTTAGTCTACTGATTCTAATCCCAATCTCTTCAGGAAAACTCTCAGA36440.15331880086521618No Hit
GGTCATGCTGTCTGTCATGTTGTGACTTTTATGCTGGGTTTTACTGAATC35870.15092056495706108No Hit
CTCTCAGAGAAACATAAATCATGTTTCATCAACTATTTGGGCATCCCTTA35820.15071019338617025No Hit
GATGAATATCCCAGCTCGGAGACAAAGTGTGTCCTACCTTTACTGTTTTG35670.15007907867349785No Hit
CATGTAACATCTCCATTTCAATGATGAACAGACATATCAAATCTAGCATG35220.14818573453548065No Hit
GATCAGTTGCAGAGGGTCTAGTTGGGAGGGAAGGAGCTGGACATGGTGGG35000.1472600996235611No Hit
GTCCACAATGTGGGTGGACATTATCCAATTCCTTGAAGGCCTGAATAGAA34630.14570334999896917No Hit
GGACATGGTGGGTGGGAAGAGGTGGGTGGCAGAAGGCAGCTTGGGAAGGG34560.14540882979972206No Hit
GAATCAACAATGGAGTGTAATGGTTAACTTTGTGTGCCAACTTGACTGGG33830.14233740486471635No Hit
AGCAGGAGGAGATGAATATCCCAGCTCGGAGACAAAGTGTGTCCTACCTT33730.14191666172293474No Hit
TGCTAGATTTGATATGTCTGTTCATCATTGAAATGGAGATGTTACATGTG33280.14002331758491754No Hit
ATGGTTAACTTTGTGTGCCAACTTGACTGGGTTAAGGGATGCCCAAATAG33000.13884523678792904No Hit
GAAAGTGAGTGATTCATCACCAAGGTCATGCTGTCTGTCATGTTGTGACT32590.13712018990662447No Hit
ATGGAGTGTAATGGTTAACTTTGTGTGCCAACTTGACTGGGTTAAGGGAT31970.13451158242757855No Hit
GTTGTGACTTTTATGCTGGGTTTTACTGAATCAACAATGGAGTGTAATGG31850.1340066906574406No Hit
GGTTTTGAGGGATGATCAAAATTTGTTTTTAGACATGCTAGATTTGATAT31550.1327444612320958No Hit
GATGAAACATGATTTATGTTTCTCTGAGAGTTTTCCTGAAGAGATTGGGA31210.13131393455003837No Hit
ACATTATCCAATTCCTTGAAGGCCTGAATAGAACAAAACAGTAAAGGTAG31050.1306407455231878No Hit
GATTGGGATTAGAATCAGTAGACTAAATAAAGAAGACCCAGAGTCCACAA30990.13038829963811882No Hit
CCTTAACCCAGTCAAGTTGGCACACAAAGTTAACCATTACACTCCATTGT30950.13022000238140619No Hit
CATAAATCATGTTTCATCAACTATTTGGGCATCCCTTAACCCAGTCAAGT30860.12984133355380276No Hit
CTTTATTTAGTCTACTGATTCTAATCCCAATCTCTTCAGGAAAACTCTCA29940.12597049664941198No Hit
GGGTCTAGTTGGGAGGGAAGGAGCTGGACATGGTGGGTGGGAAGAGGTGG29330.12340396348454422No Hit
CTCATCAGCAGCTGGCCAAAGCCCTTCCCAAGCTGCCTTCTGCCACCCAC29250.12306736897111893No Hit
GTATAAGGGAATGTGGTGCTTATGGCATTTTGCGCTGGAATTTTTAGGAA29220.12294114602858446No Hit
CCCACATTGTGGACTCTGGGTCTTCTTTATTTAGTCTACTGATTCTAATC29090.12239417994426836No Hit
GAGTGTAATGGTTAACTTTGTGTGCCAACTTGACTGGGTTAAGGGATGCC28030.11793430264138335No Hit
AATCTAGCATGTCTAAAAACAAATTTTGATCATCCCTCAAAACCTGTCAT27850.1171769649861765No Hit
GTGTCCTACCTTTACTGTTTTGTTCTATTCAGGCCTTCAAGGAATTGGAT27400.11528362084815927No Hit
AAACAATGGGAAGATATGGCTGCCATTTAATTAAATGACAGGTTTTGAGG27310.11490495202055581No Hit
GGCCAAAGCCCTTCCCAAGCTGCCTTCTGCCACCCACCTCTTCCCACCCA27280.11477872907802135No Hit
GTCCAGCTCCTTCCCTCCCAACTAGACCCTCTGCAACTGATCCCTGCAAA27200.11444213456459607No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT26950.11339027671014205No Hit
GGTTTGCAGGGATCAGTTGCAGAGGGTCTAGTTGGGAGGGAAGGAGCTGG26760.11259086474075701No Hit
CTTCTTTATTTAGTCTACTGATTCTAATCCCAATCTCTTCAGGAAAACTC26730.11246464179822252No Hit
GATCAAGAAAGAAGAGTTCTCTGAGATCATGAAGATCTGTTCCACCATTG26590.11187560139972827No Hit
ATATGTATGTGTACACATTAACACATGTAACATCTCCATTTCAATGATGA26260.11048714903184899No Hit
AGAATCAGTAGACTAAATAAAGAAGACCCAGAGTCCACAATGTGGGTGGA25940.10914077097814785No Hit
GTCTTCTTTATTTAGTCTACTGATTCTAATCCCAATCTCTTCAGGAAAAC25580.1076260956677341No Hit
GACATATCAAATCTAGCATGTCTAAAAACAAATTTTGATCATCCCTCAAA25260.10627971761403296No Hit
AACTTGACTGGGTTAAGGGATGCCCAAATAGTTGATGAAACATGATTTAT25210.10606934604314217No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT24740.10409185327676863No Hit
TCTTTATTTAGTCTACTGATTCTAATCCCAATCTCTTCAGGAACTGTCTC24680.10383940739169967No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA24580.10341866424991807No Hit
GAGAAACATAAATCATGTTTCATCAACTATTTGGGCATCCCTTAACCCAG24560.10333451562156175No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA24540.10325036699320542No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT24300.10224058345292958No Hit
ATTGGATAATGTCCACCCACATTGTGGACTCTGGGTCTTCTTTATTTAGT24190.10177776599696982No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT24090.10135702285518819No Hit
AGCGAAAGCAGGCAAAGGCCACTGGTGGGGGTTTGCAGGGATCAGTTGCA24040.1011466512842974No Hit
CACCAAGGTCATGCTGTCTGTCATGTTGTGACTTTTATGCTGGGTTTTAC23790.1000947934298434No Hit
GCAGGGGCCCAGGCTCATCAGCAGCTGGCCAAAGCCCTTCCCAAGCTGCC23790.1000947934298434No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)