FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_E9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_E9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3520944
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGAGTTTATGTAGTTCCAGAAGAGAATTGCTGGATCATATTGCAT598251.6991181910305873No Hit
ATGTATAGGAGTTTATGTAGTTCCAGAAGAGAATTGCTGGATCATATTGC501771.4251007684302848No Hit
GTATAAAGTATTAGGATACGGATCTGCAGATTCTCCAGTACACTGTTGTT452391.2848542890770203No Hit
GTATTAGGATACGGATCTGCAGATTCTCCAGTACACTGTTGTTGGTACTA429791.220666957497762No Hit
AGTTTATGTAGTTCCAGAAGAGAATTGCTGGATCATATTGCATACCACAT372431.0577561017727064No Hit
GTTGTTGGTACTATAAATTGGTGAACTTGTTACCACTTTGGAAAACTCCT327550.9302902857869935No Hit
CTCCAGTACACTGTTGTTGGTACTATAAATTGGTGAACTTGTTACCACTT321680.9136186204608763No Hit
ATATTGCATACCACATACTCAACTTTAGGAGTTTTCCAAAGTGGTAACAA308710.8767819084881782No Hit
GTTCTAGGACTCGGAGTTCATAACTCAAACCAAAGGCAGACACTTAGCTG276850.786294811845914No Hit
ATCATATTGCATACCACATACTCAACTTTAGGAGTTTTCCAAAGTGGTAA276680.7858119867853621No Hit
TCCTAATACTTTATACGACGAGACTTTAAAATCAAGCTCCTGAGTGGCTC237350.6741089889529626No Hit
CACACACACAGAGGGCACAAGCAGGCAGAGGGAGATCGAGAAGAAGCAGG215850.6130458195302169No Hit
CACACACAGAGGGCACAAGCAGGCAGAGGGAGATCGAGAAGAAGCAGGCT214140.6081891674505474No Hit
ATTTTAAAGTCTCGTCGTATAAAGTATTAGGATACGGATCTGCAGATTCT180790.5134702511599162No Hit
ACTTTATACGACGAGACTTTAAAATCAAGCTCCTGAGTGGCTCAGTCAGC175070.49722460794605083No Hit
CTGTTGTTGGTACTATAAATTGGTGAACTTGTTACCACTTTGGAAAACTC174670.49608854898004623No Hit
GTATCAGACTTCCCCTGCTCTGCGGGGAGCCTGCTTCTTCTCGATCTCCC171880.4881645376921644No Hit
ACTCAGGAGCTTGATTTTAAAGTCTCGTCGTATAAAGTATTAGGATACGG166710.47348097555655533No Hit
GTACTATAAATTGGTGAACTTGTTACCACTTTGGAAAACTCCTAAAGTTG164660.4676586733557819No Hit
CCACATACTCAACTTTAGGAGTTTTCCAAAGTGGTAACAAGTTCACCAAT158750.4508734021330643No Hit
GAGTTTATGTAGTTCCAGAAGAGAATTGCTGGATCATATTGCATACCACA155480.4415861200859769No Hit
CACACAGAGGGCACAAGCAGGCAGAGGGAGATCGAGAAGAAGCAGGCTCC155430.4414441127152263No Hit
GCATACCACATACTCAACTTTAGGAGTTTTCCAAAGTGGTAACAAGTTCA152420.432895268996042No Hit
ACACTGTTGTTGGTACTATAAATTGGTGAACTTGTTACCACTTTGGAAAA142090.40355654619897396No Hit
GTACACTGTTGTTGGTACTATAAATTGGTGAACTTGTTACCACTTTGGAA141140.400858406154713No Hit
ATAAATTGGTGAACTTGTTACCACTTTGGAAAACTCCTAAAGTTGAGTAT139800.3970526086185977No Hit
TGTTACCACTTTGGAAAACTCCTAAAGTTGAGTATGTGGTATGCAATATG139780.39699580567029746No Hit
CCAGTACACTGTTGTTGGTACTATAAATTGGTGAACTTGTTACCACTTTG136680.3881913486837621No Hit
CTATAAATTGGTGAACTTGTTACCACTTTGGAAAACTCCTAAAGTTGAGT124640.35399597380702447No Hit
GAACAGGAGAGAGGAACAGAGGGAAAGGGAGAAGGAGACTCCACAGTGAG123580.3509854175471123No Hit
CCTTTTACACGCACTCCGCGCTACGAACAAATATACCGGCGGGCTGCTCC120530.3423229679313275No Hit
ACCAACAACAGTGTACTGGAGAATCTGCAGATCCGTATCCTAATACTTTA119770.3401644558959188No Hit
GTACTGGAGAATCTGCAGATCCGTATCCTAATACTTTATACGACGAGACT116700.33144520333183375No Hit
TCCGTATCCTAATACTTTATACGACGAGACTTTAAAATCAAGCTCCTGAG110940.3150859542213679No Hit
CTCCTAAAGTTGAGTATGTGGTATGCAATATGATCCAGCAATTCTCTTCT109460.310882536047151No Hit
CTTAGCTGACTGAGCCACTCAGGAGCTTGATTTTAAAGTCTCGTCGTATA101580.28850217441686093No Hit
ACTTTAGGAGTTTTCCAAAGTGGTAACAAGTTCACCAATTTATAGTACCA101270.2876217287182074No Hit
GTCCAACACGGTACCTGAACCCAGGACTCCAGGATCAAGACCTGAGCTGA100740.2861164505882513No Hit
CTTTAGGAGTTTTCCAAAGTGGTAACAAGTTCACCAATTTATAGTACCAA100250.28472477835489574No Hit
ATGTAGTTCCAGAAGAGAATTGCTGGATCATATTGCATACCACATACTCA99840.283560317914741No Hit
GGATACGGATCTGCAGATTCTCCAGTACACTGTTGTTGGTACTATAAATT98710.28035095133577814No Hit
GGGCAAGAGAGAAGCAGACTCCCTGCTGAGCAGGAAGCCCAATGCGGGGC98530.27983972480107605No Hit
AGCGAAAGCAGGGCAAGAGAGAAGCAGACTCCCTGCTGAGCAGGAAGCCC96220.27327898427239966No Hit
ATTGCATACCACATACTCAACTTTAGGAGTTTTCCAAAGTGGTAACAAGT89120.2531139376258185No Hit
TGTTGGTACTATAAATTGGTGAACTTGTTACCACTTTGGAAAACTCCTAA88890.2524607037203659No Hit
GGATCATATTGCATACCACATACTCAACTTTAGGAGTTTTCCAAAGTGGT87880.24959215483120437No Hit
ATGCAATATGATCCAGCAATTCTCTTCTGGAACTACATAAACTCCTATAC87680.24902412534820206No Hit
AAGTATTAGGATACGGATCTGCAGATTCTCCAGTACACTGTTGTTGGTAC84700.24056048605146804No Hit
ACATAAACTCCTATACATTTTCTTTTCTTTCTTTCCTTTTTTTAAAGATT84320.23948123003376368No Hit
GTCTGATACAGGACTCGATTTCATGACCAGAGCTGAAGGCAGTGGCTCAG84170.23905520792151194No Hit
ATACTCAACTTTAGGAGTTTTCCAAAGTGGTAACAAGTTCACCAATTTAT84170.23905520792151194No Hit
ATGCTTAACTGACTGAGCCACTGCCTTCAGCTCTGGTCATGAAATCGAGT83900.23828836811945886No Hit
GTACCTGAACCCAGGACTCCAGGATCAAGACCTGAGCTGAAGGCAGATGC83170.23621506050650054No Hit
TTCCAGAAGAGAATTGCTGGATCATATTGCATACCACATACTCAACTTTA82900.23544822070444746No Hit
GAGAGAAGCAGACTCCCTGCTGAGCAGGAAGCCCAATGCGGGGCTCTGTT80810.22951231260707355No Hit
GGTCAATGATTTGTTACGAGTGAAGGGCATCGGAGTACGAGGCTTGAAGA80280.2280070344771175No Hit
GCCCAAGGCTTCGGCCTGATTGTTCGATTCGAAATGCTGAACGCCGGGAC79770.2265585592954617No Hit
GATCTGCAGATTCTCCAGTACACTGTTGTTGGTACTATAAATTGGTGAAC79560.22596212833830928No Hit
AGCGAAAGCAGGTCAATGATTTGTTACGAGTGAAGGGCATCGGAGTACGA79290.22519528853625617No Hit
ACTATAAATTGGTGAACTTGTTACCACTTTGGAAAACTCCTAAAGTTGAG78380.22261075438859576No Hit
GAGTACGAGGCTTGAAGAAGCTCATGCCGCACGTCGTGCTCGACCCGCCC75910.21559559027351755No Hit
GTGGTAACAAGTTCACCAATTTATAGTACCAACAACAGTGTACTGGAGAA73810.20963128070199355No Hit
CAAATATACCGGCGGGCTGCTCCGAGCGCGGCCCGCCGGTCCCGGCGTTC73490.20872243352918987No Hit
GCCCAATGCGGGGCTCTGTTCTAGGACTCGGAGTTCATAACTCAAACCAA71210.20224689742296384No Hit
GGAGAGAGGAACAGAGGGAAAGGGAGAAGGAGACTCCACAGTGAGCAGGG70880.20130964877601007No Hit
GAAAATGTATAGGAGTTTATGTAGTTCCAGAAGAGAATTGCTGGATCATA70670.20071321781885768No Hit
GTTTATGTAGTTCCAGAAGAGAATTGCTGGATCATATTGCATACCACATA68940.1957997627908879No Hit
GTGGTATGCAATATGATCCAGCAATTCTCTTCTGGAACTACATAAACTCC68730.19520333183373548No Hit
AGTATGTGGTATGCAATATGATCCAGCAATTCTCTTCTGGAACTACATAA68720.19517493035958539No Hit
GTATATTTGTTCGTAGCGCGGAGTGCGTGTAAAAGGAGCGCTTCAGCGTG68000.19313002422077716No Hit
TTTCCAAAGTGGTAACAAGTTCACCAATTTATAGTACCAACAACAGTGTA66960.19017627090916528No Hit
CTGTGTGTGTGTGTCAAATAAATAAATATAATCTTTAAAAAAAGGAAAGA66590.18912541636561103No Hit
GCCTTCAGCTCTGGTCATGAAATCGAGTCCTGTATCAGACTTCCCCTGCT65610.18634207189889984No Hit
TTATAGTACCAACAACAGTGTACTGGAGAATCTGCAGATCCGTATCCTAA65610.18634207189889984No Hit
GCTCTGCGGGGAGCCTGCTTCTTCTCGATCTCCCTCTGCCTGCTTGTGCC65520.18608645863154882No Hit
GTTAAGCATCTGCCTTCAGCTCAGGTCTTGATCCTGGAGTCCTGGGTTCA65190.18514920998459505No Hit
GTGTGTGTGTGTCAAATAAATAAATATAATCTTTAAAAAAAGGAAAGAAA64660.18364393185463898No Hit
CTCCTGAGTGGCTCAGTCAGCTAAGTGTCTGCCTTTGGTTTGAGTTATGA61870.1757199205667571No Hit
GGCAGAGGGAGATCGAGAAGAAGCAGGCTCCCCGCAGAGCAGGGGAAGTC59760.16972720952108297No Hit
AAAGCAGGGAGGCTCGCAGCTTGCTGGCGGGTGGATCGAGCGGTCGGCCC59250.16827873433942717No Hit
TCATAACTCAAACCAAAGGCAGACACTTAGCTGACTGAGCCACTCAGGAG59090.1678243107530253No Hit
ACTCCACAGTGAGCAGGGAGTCCAACACGGTACCTGAACCCAGGACTCCA57900.16444453532916173No Hit
GATTTGTTACGAGTGAAGGGCATCGGAGTACGAGGCTTGAAGAAGCTCAT55680.15813940806783636No Hit
CACTTAGCTGACTGAGCCACTCAGGAGCTTGATTTTAAAGTCTCGTCGTA54870.1558388886616771No Hit
CTCCTATACATTTTCTTTTCTTTCTTTCCTTTTTTTAAAGATTATATTTA54870.1558388886616771No Hit
CTTCAGCTCTGGTCATGAAATCGAGTCCTGTATCAGACTTCCCCTGCTCT54810.15566847981677642No Hit
GGGCACAAGCAGGCAGAGGGAGATCGAGAAGAAGCAGGCTCCCCGCAGAG54150.15379398252286886No Hit
GCTCAGTGAGGTTGCAAGCACGCTGCAGCTGACGATGGCGAAGGTTCTGG54110.1536803766262684No Hit
CCCCTGCTCTGCGGGGAGCCTGCTTCTTCTCGATCTCCCTCTGCCTGCTT53530.1520330911255618No Hit
GAGTATGTGGTATGCAATATGATCCAGCAATTCTCTTCTGGAACTACATA52970.1504426085731554No Hit
CTCTGTGTGTGTGTGTCAAATAAATAAATATAATCTTTAAAAAAAGGAAA52340.14865331570169818No Hit
CTGTTCTAGGACTCGGAGTTCATAACTCAAACCAAAGGCAGACACTTAGC52150.148113687692846No Hit
CTTTGGAAAACTCCTAAAGTTGAGTATGTGGTATGCAATATGATCCAGCA51840.14723324199419247No Hit
GTTCCAGAAGAGAATTGCTGGATCATATTGCATACCACATACTCAACTTT51080.1450747299587838No Hit
TTGCATACCACATACTCAACTTTAGGAGTTTTCCAAAGTGGTAACAAGTT51030.1449327225880332No Hit
CAACAACAGTGTACTGGAGAATCTGCAGATCCGTATCCTAATACTTTATA50780.14422268573428035No Hit
AATTTATAGTACCAACAACAGTGTACTGGAGAATCTGCAGATCCGTATCC49920.1417801589573705No Hit
CTTGTTACCACTTTGGAAAACTCCTAAAGTTGAGTATGTGGTATGCAATA49820.14149614421586937No Hit
GTGTACTGGAGAATCTGCAGATCCGTATCCTAATACTTTATACGACGAGA49720.14121212947436823No Hit
AACCAAAGGCAGACACTTAGCTGACTGAGCCACTCAGGAGCTTGATTTTA49250.13987726018931285No Hit
GTGAGCAGGGAGTCCAACACGGTACCTGAACCCAGGACTCCAGGATCAAG49030.13925242775801033No Hit
GAGCTGAAGGCAGTGGCTCAGTCAGTTAAGCATCTGCCTTCAGCTCAGGT48960.13905361743895955No Hit
CACAGAGGGCACAAGCAGGCAGAGGGAGATCGAGAAGAAGCAGGCTCCCC48710.13834358058520668No Hit
GAAAGAAAAGAAAATGTATAGGAGTTTATGTAGTTCCAGAAGAGAATTGC48560.13791755847295498No Hit
GTTCTGGCTCGAGGCAGCACGCTGAAGCGCTCCTTTTACACGCACTCCGC48340.13729272604165246No Hit
TTGCTGGATCATATTGCATACCACATACTCAACTTTAGGAGTTTTCCAAA47690.13544663022189504No Hit
AGCAGGAACAGGAGAGAGGAACAGAGGGAAAGGGAGAAGGAGACTCCACA47230.13414016241098978No Hit
GAACAGAGGGAAAGGGAGAAGGAGACTCCACAGTGAGCAGGGAGTCCAAC46540.1321804606946319No Hit
AGTGAAGGGCATCGGAGTACGAGGCTTGAAGAAGCTCATGCCGCACGTCG46430.13186804447898062No Hit
TGTAAAAGGAGCGCTTCAGCGTGCTGCCTCGAGCCAGAACCTTCGCCATC45980.1305899781422255No Hit
GTTCATAACTCAAACCAAAGGCAGACACTTAGCTGACTGAGCCACTCAGG45650.12965272949527173No Hit
GAAGAGAATTGCTGGATCATATTGCATACCACATACTCAACTTTAGGAGT45600.12951072212452117No Hit
CTCCCTGCTGAGCAGGAAGCCCAATGCGGGGCTCTGTTCTAGGACTCGGA45170.12828945873606623No Hit
ACACTTAGCTGACTGAGCCACTCAGGAGCTTGATTTTAAAGTCTCGTCGT45030.12789183809796464No Hit
GAAAAGAAAATGTATAGGAGTTTATGTAGTTCCAGAAGAGAATTGCTGGA44690.12692618797686075No Hit
CCTATACATTTTCTTTTCTTTCTTTCCTTTTTTTAAAGATTATATTTATT43340.12309198896659532No Hit
GACTGAGCCACTGCCTTCAGCTCTGGTCATGAAATCGAGTCCTGTATCAG43330.1230635874924452No Hit
GAGAGGAACAGAGGGAAAGGGAGAAGGAGACTCCACAGTGAGCAGGGAGT43320.12303518601829509No Hit
CTCCAGGATCAAGACCTGAGCTGAAGGCAGATGCTTAACTGACTGAGCCA43250.1228363756992443No Hit
TATTGCATACCACATACTCAACTTTAGGAGTTTTCCAAAGTGGTAACAAG43180.12263756538019349No Hit
ACAGTGTACTGGAGAATCTGCAGATCCGTATCCTAATACTTTATACGACG43080.12235355063869235No Hit
GCTCTGTTCTAGGACTCGGAGTTCATAACTCAAACCAAAGGCAGACACTT42730.12135949904343835No Hit
GGCAGACACTTAGCTGACTGAGCCACTCAGGAGCTTGATTTTAAAGTCTC42600.12099027987948686No Hit
ACCACATACTCAACTTTAGGAGTTTTCCAAAGTGGTAACAAGTTCACCAA42510.12073466661213583No Hit
ATATTTATTTATTTGACACACACACACAGAGGGCACAAGCAGGCAGAGGG42420.1204790533447848No Hit
TCCTAGAACAGAGCCCCGCATTGGGCTTCCTGCTCAGCAGGGAGTCTGCT42420.1204790533447848No Hit
GTATGCAATATGATCCAGCAATTCTCTTCTGGAACTACATAAACTCCTAT42040.11939979732708048No Hit
TACACGCACTCCGCGCTACGAACAAATATACCGGCGGGCTGCTCCGAGCG41330.11738329266242234No Hit
AGCGAAAGCAGGGAGGCTCGCAGCTTGCTGGCGGGTGGATCGAGCGGTCG40830.11596321895491664No Hit
CTCAGGAGCTTGATTTTAAAGTCTCGTCGTATAAAGTATTAGGATACGGA40620.11536678799776423No Hit
TACTCAACTTTAGGAGTTTTCCAAAGTGGTAACAAGTTCACCAATTTATA39870.11323667743650566No Hit
GAGAAGCAGACTCCCTGCTGAGCAGGAAGCCCAATGCGGGGCTCTGTTCT39750.11289585974670428No Hit
ATGCGGGGCTCTGTTCTAGGACTCGGAGTTCATAACTCAAACCAAAGGCA38980.11070894623714549No Hit
GAAGCAGACTCCCTGCTGAGCAGGAAGCCCAATGCGGGGCTCTGTTCTAG38910.11051013591809469No Hit
CTTTAAAATCAAGCTCCTGAGTGGCTCAGTCAGCTAAGTGTCTGCCTTTG38810.11022612117659356No Hit
GCTGAAGGCAGATGCTTAACTGACTGAGCCACTGCCTTCAGCTCTGGTCA38510.10937407695209012No Hit
GGTAACAAGTTCACCAATTTATAGTACCAACAACAGTGTACTGGAGAATC38320.10883444894323796No Hit
ATCAAGACCTGAGCTGAAGGCAGATGCTTAACTGACTGAGCCACTGCCTT38270.10869244157248738No Hit
GATTATATTTATTTATTTGACACACACACACAGAGGGCACAAGCAGGCAG38180.10843682830513635No Hit
GACTGAGCCACTCAGGAGCTTGATTTTAAAGTCTCGTCGTATAAAGTATT38040.10803920766703475No Hit
TTGGTGAACTTGTTACCACTTTGGAAAACTCCTAAAGTTGAGTATGTGGT37930.10772679145138349No Hit
AAATTGGTGAACTTGTTACCACTTTGGAAAACTCCTAAAGTTGAGTATGT37650.1069315501751803No Hit
CTGCTGAGCAGGAAGCCCAATGCGGGGCTCTGTTCTAGGACTCGGAGTTC37000.10508545435542287No Hit
GAATTGCTGGATCATATTGCATACCACATACTCAACTTTAGGAGTTTTCC36980.10502865140712264No Hit
CCAATTTATAGTACCAACAACAGTGTACTGGAGAATCTGCAGATCCGTAT36840.10463103076902103No Hit
ACCCAGGACTCCAGGATCAAGACCTGAGCTGAAGGCAGATGCTTAACTGA36760.10440381897582013No Hit
ATTTATAGTACCAACAACAGTGTACTGGAGAATCTGCAGATCCGTATCCT36700.10423341013091944No Hit
GGGAAAGGGAGAAGGAGACTCCACAGTGAGCAGGGAGTCCAACACGGTAC36240.10292694232001418No Hit
GGGAGAAGGAGACTCCACAGTGAGCAGGGAGTCCAACACGGTACCTGAAC36080.10247251873361236No Hit
ACTCAAACCAAAGGCAGACACTTAGCTGACTGAGCCACTCAGGAGCTTGA36040.10235891283701189No Hit
GTCAGCTAAGTGTCTGCCTTTGGTTTGAGTTATGAACTCCGAGTCCTAGA35990.10221690546626133No Hit
GCCTTGGGCGGGTCGAGCACGACGTGCGGCATGAGCTTCTTCAAGCCTCG35890.10193289072476018No Hit
GTTGAGTATGTGGTATGCAATATGATCCAGCAATTCTCTTCTGGAACTAC35760.1015636715608087No Hit
GACTTCCCCTGCTCTGCGGGGAGCCTGCTTCTTCTCGATCTCCCTCTGCC35710.10142166419005812No Hit
TTCTCCAGTACACTGTTGTTGGTACTATAAATTGGTGAACTTGTTACCAC35610.10113764944855697No Hit
GTTACGAGTGAAGGGCATCGGAGTACGAGGCTTGAAGAAGCTCATGCCGC35420.10059802143970481No Hit
GAGCAGGGGAAGTCTGATACAGGACTCGATTTCATGACCAGAGCTGAAGG35370.10045601406895423No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)