FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_E8a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_E8a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1362568
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAAGACTGAATGGAAACTGAACGGACAGGTGCTGGTCTTCACACTCC59090.43366642985891346No Hit
AAGCAGGACAGTGGGAGACAGGAAACGGCAAAAACGGCAGTGGCTGAGAC59090.43366642985891346No Hit
GTGGGAGACAGGAAACGGCAAAAACGGCAGTGGCTGAGACCTGACAGCAA39100.2869581554828823No Hit
CTCATATACTGCACCCATTTCAGCAGCAAATCCTGTTGCCCCTACTTTCA38510.2826280963592276No Hit
GTATATGAGAAGAAAAAAGAAATGCAGAATAACACAAAGATTCTAGACAA34370.25224429166104No Hit
GTAATAAGCCAATGAGTTGGAATCCTTGATGAAAATGCCCTCATACTGCA32450.23815325180101105No Hit
CCCTCATACTGCAGCTTCTGTTTCCCTGCAGGCATACCCGTGGCTTCGTG26360.19345823474498153No Hit
GTCCATCAAAGTCCAAGTGCCCAACATGCAGGATAAGACTGAATGGAAAC25080.18406420817162886No Hit
AAACAAGGGTCCAGTGTCCATCAAAGTCCAAGTGCCCAACATGCAGGATA24780.18186248319349935No Hit
GTCCAGTGTCCATCAAAGTCCAAGTGCCCAACATGCAGGATAAGACTGAA24250.1779727690654705No Hit
GACCAGGTCTCTGTCATCAAGGTGAAGATTCACGAAGCCACGGGTATGCC23400.1717345482941035No Hit
GTGCTGGTCTTCACACTCCCGCTCACAGACCAGGTCTCTGTCATCAAGGT23260.17070707663764303No Hit
CTCCTTGAGGGCCAGGTGGATGATGGCGCCGTTGGCCATGTTGTAATAAG22620.1660100633509667No Hit
GTTGTAATAAGCCAATGAGTTGGAATCCTTGATGAAAATGCCCTCATACT21320.15646925511240542No Hit
ACCTAGGAGACACCAGTGGCTTGGACCAGCATAGCTGAAGCACACACGGT20720.15206580515614632No Hit
GTATGAGGGCATTTTCATCAAGGATTCCAACTCATTGGCTTATTACAACA20690.15184563265833337No Hit
GGCTGAGACCTGACAGCAAGTTTCTCTTCTCTACTTCTTCCGCCCGCCTC20600.15118511516489452No Hit
GGCATACCCGTGGCTTCGTGAATCTTCACCTTGATGACAGAGACCTGGTC17910.13144298119433304No Hit
GTAGAAACAAGGGTCCAGTGTCCATCAAAGTCCAAGTGCCCAACATGCAG17850.13100263619870717No Hit
ATTCTAGACAATGAGCTTTTTCTTTCCAGACACAGGTGCTGCTCCTTCTT16930.12425067959910992No Hit
TGTTGTAATAAGCCAATGAGTTGGAATCCTTGATGAAAATGCCCTCATAC16830.12351677127306673No Hit
GTCTAGAATCTTTGTGTTATTCTGCATTTCTTTTTTCTTCTCATATACTG16760.12300303544483653No Hit
CTCTTAATCTTGGCTTAAATAAATAGGGTGCTTATTTTTCTTCTGTAAGA16600.12182878212316742No Hit
CCATAAAATTCGCCCTTTTAGAACTGTACAACTCAGTGATTTTAGTCTTT16080.1180124588277429No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA15590.11441630803013135No Hit
GTATGTTATTGTCCTCATGCTTGTCTCTGTAGGTTACCAAATACCTGTTC15260.11199441055418885No Hit
GACTATAGGCACGCCAGAGTGGGAGCAGGGAGAGGAAACATTGGCCATTA15040.11037981223689386No Hit
GACAGGTGCTGGTCTTCACACTCCCGCTCACAGACCAGGTCTCTGTCATC14950.109719294743455No Hit
TCACACTCCCGCTCACAGACCAGGTCTCTGTCATCAAGGTGAAGATTCAC14930.10957251307824638No Hit
GGTCTCTGTCATCAAGGTGAAGATTCACGAAGCCACGGGTATGCCTGCAG14920.10949912224564205No Hit
ATAATAACCTAGGAGACACCAGTGGCTTGGACCAGCATAGCTGAAGCACA14910.10942573141303773No Hit
GTGGATGATGGCGCCGTTGGCCATGTTGTAATAAGCCAATGAGTTGGAAT14900.10935234058043343No Hit
CCTCTCTCCTTGAGGGCCAGGTGGATGATGGCGCCGTTGGCCATGTTGTA14650.10751756976532546No Hit
ATAACATACTAAGGATTTCTGAATGGAAAGGCAGAAATAGCCTCCTGGCT14450.10604975311323912No Hit
GGGTATGCCTGCAGGGAAACAGAAGCTGCAGTATGAGGGCATTTTCATCA14390.1056094081176132No Hit
GTTCTAAAAGGGCGAATTTTATGGTATGTGAATTATGTCTTAATAAAAAA14120.10362785563729664No Hit
GAGTTGGAATCCTTGATGAAAATGCCCTCATACTGCAGCTTCTGTTTCCC13890.10193986648739733No Hit
GAATGGAAACTGAACGGACAGGTGCTGGTCTTCACACTCCCGCTCACAGA13830.10149952149177141No Hit
CTCCTAGGTTATTATAATGGCCAATGTTTCCTCTCCCTGCTCCCACTCTG13790.10120595816135415No Hit
GGGTATATGGAATCTATATCAAACAAGAAAGGCAGGAGGAAGATAGAAGC13790.10120595816135415No Hit
GGTGGATGATGGCGCCGTTGGCCATGTTGTAATAAGCCAATGAGTTGGAA13690.10047204983531097No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)