FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_E7a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_E7a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1141968
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAGCAAAGCTCACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTTT78490.6873222367001527No Hit
ATCCTGGAGTCCTGGGACCGAGTCCTACGTCAGGCTCCCTGTTCGGCGGG68700.6015930393846413No Hit
GAGAGAGAGAGTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCG66960.5863561851120171No Hit
GTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGG61880.5418715760861951No Hit
CACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTTTTATGTCAGTCAAG61160.5355666708699368No Hit
GCTCACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTTTTATGTCAGTC59660.5224314516693987No Hit
ATTTGAGAGAGAGAGTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTC57590.5043048491726564No Hit
GTCCCAGGACTCCAGGATCATGACCTGAGCCAAAGGCAGTTGCTCAACCA56720.4966864220363442No Hit
GGTCATGATCCTGGAGTCCTGGGACCGAGTCCTACGTCAGGCTCCCTGTT54870.48048631835568073No Hit
GGTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGGAGCCTGACGTAGGAC54030.47313059560337944No Hit
AGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGGAGCCT53520.4686646210751965No Hit
GTCCTGGGACCGAGTCCTACGTCAGGCTCCCTGTTCGGCGGGGAGTCTGC52850.46279755649895615No Hit
CTTTTATGTCAGTCAAGAAAGCAGAAATGAGGGGCACCTGGGTGGCTCAG52240.4574559006907374No Hit
CTCCAGGATCATGACCTGAGCCAAAGGCAGTTGCTCAACCAACTGAGCCA52180.45693049192271584No Hit
GAAATGAGGGGCGCCTGGGTGGCTCAGTTGGTTAAGCCGCTGCAGCTCGG49680.4350384599218192No Hit
ATCATGACCTGAGCCAAAGGCAGTTGCTCAACCAACTGAGCCACCCAGGT48320.42312919451333136No Hit
ACGTAGGACTCGGTCCCAGGACTCCAGGATCATGACCTGAGCCAAAGGCA44880.3930057584800975No Hit
GTCCTACGTCAGGCTCCCTGTTCGGCGGGGAGTCTGCGCTTCCTCTGACC43870.38416137755173524No Hit
GGCATAGTGGCAGGCAGAAGCAGAGGGAGACATAGGCTCCCTGCTGAGCA40330.35316226023846553No Hit
GCTTTTATGTCAGTCAAGAAAGCAGAAATGAGGGGCACCTGGGTGGCTCA39360.34466815182211763No Hit
CCCTGAGATCATGACCCGAGCTGCAGCGGCTTAACCAACTGAGCCACCCA37940.3322334776456083No Hit
GACATAGGCTCCCTGCTGAGCAAGGATCCTCTGGTGGGACTCCATCTCCA37330.3268918218373895No Hit
GAAATGAGGGGCACCTGGGTGGCTCAGTTGGTTGAGCAACTGCCTTTGGC36160.3166463508609698No Hit
GAGTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAACAG36160.3166463508609698No Hit
CGCCTGGGTGGCTCAGTTGGTTAAGCCGCTGCAGCTCGGGTCATGATCTC34780.3045619491964749No Hit
CACCTGGGTGGCTCAGTTGGTTGAGCAACTGCCTTTGGCTCAGGTCATGA34680.303686267916439No Hit
GAGAGAGAGTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCC34450.3016722009723565No Hit
GGGATAGCAAAGCTCACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTT33720.2952797276280947No Hit
GTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGGAGCCTGACGTAGGACT33510.29344079694001934No Hit
GCCTTTGGCTCAGGTCATGATCCTGGAGTCCTGGGACCGAGTCCTACGTC31480.27566446695529123No Hit
ATTTATTTGAGAGAGAGAGTGTGAGAATGGAGAGGTCAGAGGAAGCGCAG31020.27163633306712626No Hit
AAAGCAGGGATAGCAAAGCTCACAAGAACGTGTCAGGGAAAACTGGCTGT30830.2699725386350581No Hit
TTCTCACACTCTCTCTCTCAAATAAATAAATAAAATTTAAAAAAAAAAAA30150.26401790593081415No Hit
TCTCCAGACCCTGAGATCATGACCCGAGCTGCAGCGGCTTAACCAACTGA29560.25885138637860255No Hit
CTCCAGACCCTGAGATCATGACCCGAGCTGCAGCGGCTTAACCAACTGAG29040.25429784372241604No Hit
ATCATGACCCGAGCTGCAGCGGCTTAACCAACTGAGCCACCCAGGCGCCC27750.24300155520995334No Hit
ATCTCAGGGTCTGGAGATGGAGTCCCACCAGAGGATCCTTGCTCAGCAGG27440.24028694324184216No Hit
GCATAGTGGCAGGCAGAAGCAGAGGGAGACATAGGCTCCCTGCTGAGCAA26370.23091715354545836No Hit
AGCAGGGATAGCAAAGCTCACAAGAACGTGTCAGGGAAAACTGGCTGTGA26170.2291657909853866No Hit
CTCCATTCTCACACTCTCTCTCTCAAATAAATAAATAAAATTTAAAAAAA26090.22846524596135792No Hit
TAGCAAAGCTCACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTTTTAT25270.22128465946506382No Hit
GGTTAAGCCGCTGCAGCTCGGGTCATGATCTCAGGGTCTGGAGATGGAGT25040.21927059252098133No Hit
GTGGCAGGCAGAAGCAGAGGGAGACATAGGCTCCCTGCTGAGCAAGGATC23890.20920025780056883No Hit
AGCGAAAGCAGGGATAGCAAAGCTCACAAGAACGTGTCAGGGAAAACTGG23670.20727375898448994No Hit
GCAGAAATGAGGGGCACCTGGGTGGCTCAGTTGGTTGAGCAACTGCCTTT23140.20263264820029986No Hit
GGTCATGATCTCAGGGTCTGGAGATGGAGTCCCACCAGAGGATCCTTGCT23010.20149426253625322No Hit
AGCAGGCATAGTGGCAGGCAGAAGCAGAGGGAGACATAGGCTCCCTGCTG21510.18835904333571518No Hit
GGGTGGCTCAGTTGGTTGAGCAACTGCCTTTGGCTCAGGTCATGATCCTG21070.18450604570355736No Hit
GGCAGAAGCAGAGGGAGACATAGGCTCCCTGCTGAGCAAGGATCCTCTGG20720.18144116122343182No Hit
GGGAAAACTGGCTGTGAGCTTTTATGTCAGTCAAGAAAGCAGAAATGAGG20590.1803027755593852No Hit
GCACCTGGGTGGCTCAGTTGGTTGAGCAACTGCCTTTGGCTCAGGTCATG20580.1802152074313816No Hit
GCCAAAGGCAGTTGCTCAACCAACTGAGCCACCCAGGTGCCCCTCATTTC20570.18012763930337802No Hit
AAAGCAGGCATAGTGGCAGGCAGAAGCAGAGGGAGACATAGGCTCCCTGC20400.17863898112731705No Hit
ACCCAGGTGCCCCTCATTTCTGCTTTCTTGACTGACATAAAAGCTCACAG19760.1730346209350875No Hit
GCGCCTGGGTGGCTCAGTTGGTTAAGCCGCTGCAGCTCGGGTCATGATCT19570.17137082650301935No Hit
GTGTCAGGGAAAACTGGCTGTGAGCTTTTATGTCAGTCAAGAAAGCAGAA19380.1697070320709512No Hit
TGTCAGGGAAAACTGGCTGTGAGCTTTTATGTCAGTCAAGAAAGCAGAAA19250.16856864640690458No Hit
CATAGGCTCCCTGCTGAGCAAGGATCCTCTGGTGGGACTCCATCTCCAGA18570.16261401370266068No Hit
GAGGAAGCGCAGACTCCCCGCCGAACAGGGAGCCTGACGTAGGACTCGGT18470.1617383324226248No Hit
GCTCAGTTGGTTGAGCAACTGCCTTTGGCTCAGGTCATGATCCTGGAGTC18280.16007453799055665No Hit
AGCGCAGACTCCCCGCCGAACAGGGAGCCTGACGTAGGACTCGGTCCCAG18110.15858587981449568No Hit
GAGGGAGACATAGGCTCCCTGCTGAGCAAGGATCCTCTGGTGGGACTCCA18060.15814803917447773No Hit
CTCCCCGCCGAACAGGGAGCCTGACGTAGGACTCGGTCCCAGGACTCCAG17980.15744749415044904No Hit
GAGAGAGTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGA17890.15665938099841675No Hit
CCTCTGACCTCTCCATTCTCACACTCTCTCTCTCAAATAAATAAATAAAA17710.15508315469435222No Hit
GGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGGAGCCTGACGT17630.15438260967032352No Hit
AAAGAAATGAGGGGCGCCTGGGTGGCTCAGTTGGTTAAGCCGCTGCAGCT17160.15026690765415493No Hit
GTTGAGCAACTGCCTTTGGCTCAGGTCATGATCCTGGAGTCCTGGGACCG17010.14895338573410113No Hit
TCCTCTGACCTCTCCATTCTCACACTCTCTCTCTCAAATAAATAAATAAA16770.14685175066201506No Hit
GAAAGAAAGAAAGAAATGAGGGGCGCCTGGGTGGCTCAGTTGGTTAAGCC16510.1445749793339218No Hit
GGGAGACATAGGCTCCCTGCTGAGCAAGGATCCTCTGGTGGGACTCCATC16290.1426484805178429No Hit
CTGCTGAGCAAGGATCCTCTGGTGGGACTCCATCTCCAGACCCTGAGATC16270.14247334426183572No Hit
GAAAGAAAGAAATGAGGGGCGCCTGGGTGGCTCAGTTGGTTAAGCCGCTG16050.1405468454457568No Hit
GAAAGAAATGAGGGGCGCCTGGGTGGCTCAGTTGGTTAAGCCGCTGCAGC15880.13905818726969582No Hit
CCTCTGGTGGGACTCCATCTCCAGACCCTGAGATCATGACCCGAGCTGCA15870.13897061914169223No Hit
AATGAGGGGCGCCTGGGTGGCTCAGTTGGTTAAGCCGCTGCAGCTCGGGT15860.13888305101368864No Hit
GAGCAAGGATCCTCTGGTGGGACTCCATCTCCAGACCCTGAGATCATGAC15410.13494248525352726No Hit
GGGTGGCTCAGTTGGTTAAGCCGCTGCAGCTCGGGTCATGATCTCAGGGT15190.13301598643744833No Hit
GAGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGGAGCC15170.13284085018144115No Hit
GTCAAGAAAGCAGAAATGAGGGGCACCTGGGTGGCTCAGTTGGTTGAGCA14490.12688621747719728No Hit
GAACGTGTCAGGGAAAACTGGCTGTGAGCTTTTATGTCAGTCAAGAAAGC14460.1266235130931865No Hit
GATCATGACCCGAGCTGCAGCGGCTTAACCAACTGAGCCACCCAGGCGCC14360.12574783181315063No Hit
AGCGAAAGCAGGCATAGTGGCAGGCAGAAGCAGAGGGAGACATAGGCTCC14200.12434674176509324No Hit
GCAAAGCTCACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTTTTATGT14180.12417160550908606No Hit
GGCTTAACCAACTGAGCCACCCAGGCGCCCCTCATTTCTTTCTTTCTTTC14140.12382133299707171No Hit
GAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGGAGCCTG13610.11918022221288163No Hit
CAGTTGGTTGAGCAACTGCCTTTGGCTCAGGTCATGATCCTGGAGTCCTG13250.11602776960475249No Hit
AACTGGCTGTGAGCTTTTATGTCAGTCAAGAAAGCAGAAATGAGGGGCAC13170.1153272245807238No Hit
GCGCAGACTCCCCGCCGAACAGGGAGCCTGACGTAGGACTCGGTCCCAGG13100.11471424768469869No Hit
GAGAGTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAAC13080.11453911142869151No Hit
ACGTCAGGCTCCCTGTTCGGCGGGGAGTCTGCGCTTCCTCTGACCTCTCC12860.11261261261261261No Hit
GGCTCAGGTCATGATCCTGGAGTCCTGGGACCGAGTCCTACGTCAGGCTC12430.1088471831084584No Hit
GGTGGGACTCCATCTCCAGACCCTGAGATCATGACCCGAGCTGCAGCGGC12410.10867204685245122No Hit
GAGCTGCAGCGGCTTAACCAACTGAGCCACCCAGGCGCCCCTCATTTCTT12260.10735852493239739No Hit
AGTCAAGAAAGCAGAAATGAGGGGCACCTGGGTGGCTCAGTTGGTTGAGC12260.10735852493239739No Hit
AGGGAAAACTGGCTGTGAGCTTTTATGTCAGTCAAGAAAGCAGAAATGAG12200.10683311616437588No Hit
GAAGCAGAGGGAGACATAGGCTCCCTGCTGAGCAAGGATCCTCTGGTGGG12020.10525688986031131No Hit
GTCTGGAGATGGAGTCCCACCAGAGGATCCTTGCTCAGCAGGGAGCCTAT11980.10490661734829697No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG11880.10403093606826111No Hit
CTGCAGCTCGGGTCATGATCTCAGGGTCTGGAGATGGAGTCCCACCAGAG11750.10289255040421448No Hit
AATGAGGGGCACCTGGGTGGCTCAGTTGGTTGAGCAACTGCCTTTGGCTC11730.10271741414820731No Hit
CTCTCTCTCAAATAAATAAATAAAATTTAAAAAAAAAAAAAGAAAGAAAG11720.10262984602020372No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA11700.10245470976419654No Hit
GCTCAGTTGGTTAAGCCGCTGCAGCTCGGGTCATGATCTCAGGGTCTGGA11560.10122875597214634No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)