FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_E7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_E7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3965826
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATTGCTGTGCATGATGGAGCGCACTGGCAAGCACTACAGCATTTTG229270.5781141179668497No Hit
GTCCAGGTCAGAAAGGGCCAGTGCCAGGGAGAACTGAAAGGAGGGAACAG170950.43105774181721535No Hit
GTAGGAGGAAGGGAGTGGAGAAAGAAGAGAGGAATCAAAGGCCACGGGGG148730.37502906078078063No Hit
GCCCTAGGTTTTGGCCTCAGCCACTGGGAAGATGGTGATGGCATTTACTG133440.33647467135471903No Hit
CTACAGCATTTTGAATAATTAGTGTCAGCCATTCTTCCTGTCCTCTTGCA118750.2994332076092093No Hit
TCCTCCTACTGTCTGCTTCCCCATAAGTCCTGGGGCTCTAGTGTCTGTTC118260.29819765163675865No Hit
GTATTCAGTAAATGCCATCACCATCTTCCCAGTGGCTGAGGCCAAAACCT115650.29161642492635836No Hit
GTGCCAGGGAGAACTGAAAGGAGGGAACAGACACTAGAGCCCCAGGACTT113610.28647247761248223No Hit
CAGTAGGAGGAAGGGAGTGGAGAAAGAAGAGAGGAATCAAAGGCCACGGG102720.25901287651046717No Hit
ACATAGTTCAGTCTGTATTCAGTAAATGCCATCACCATCTTCCCAGTGGC100410.2531881126403428No Hit
GCAATACATAGTTCAGTCTGTATTCAGTAAATGCCATCACCATCTTCCCA93780.23647028387024543No Hit
ACTGAATACAGACTGAACTATGTATTGCTGTGCATGATGGAGCGCACTGG93010.23452869591353728No Hit
GAACTATGTATTGCTGTGCATGATGGAGCGCACTGGCAAGCACTACAGCA90370.2278718229191094No Hit
GAATTGGATGGGCGTAGTAGGTATTTTGGAAGGTGAACAGATGGGGCTTA89930.22676234408670476No Hit
ACCTAGGGCTCATCCTCAAATCCTCTGCTGCCATGCAACCCTCTTCAGTA88100.22214792076102177No Hit
GGCTGGGACTGGGGGGCCGAGGCCGAGAAACACGCAGCGGGGCTGGGCAG83440.21039753130873617No Hit
GGCAAGCACTACAGCATTTTGAATAATTAGTGTCAGCCATTCTTCCTGTC81510.20553095370296126No Hit
ATGCTGTAGTGCTTGCCAGTGCGCTCCATCATGCACAGCAATACATAGTT81340.20510229142680492No Hit
CCCCAGGACTTATGGGGAAGCAGACAGTAGGAGGAAGGGAGTGGAGAAAG74270.18727498382430294No Hit
GGTCAGAAAGGGCCAGTGCCAGGGAGAACTGAAAGGAGGGAACAGACACT73050.18419870160718094No Hit
CACTAGAGCCCCAGGACTTATGGGGAAGCAGACAGTAGGAGGAAGGGAGT71080.17923126228936923No Hit
ATCAAAGGCCACGGGGGTTTTAACCTGCAAGAGGACAGGAAGAATGGCTG70970.17895389258126806No Hit
GTGCATGATGGAGCGCACTGGCAAGCACTACAGCATTTTGAATAATTAGT69110.1742638229715575No Hit
GCATGATGGAGCGCACTGGCAAGCACTACAGCATTTTGAATAATTAGTGT63810.1608996461266833No Hit
TTACTGAAGAGGGTTGCATGGCAGCAGAGGATTTGAGGATGAGCCCTAGG63710.16064749184659136No Hit
CCCTGGCACTGGCCCTTTCTGACCTGGACTGTGGCAAAGGCTCCGGTGCA63350.15973973643826028No Hit
GAGGAAGGGAGTGGAGAAAGAAGAGAGGAATCAAAGGCCACGGGGGTTTT62660.15799987190562573No Hit
GACCTGGACTGTGGCAAAGGCTCCGGTGCAAGTCTCGCTGCCCAGCCCCG60970.1537384645720715No Hit
CTCCTACTGTCTGCTTCCCCATAAGTCCTGGGGCTCTAGTGTCTGTTCCC60660.1529567863037864No Hit
GGGCTGGGCAGCGAGACTTGCACCGGAGCCTTTGCCACAGTCCAGGTCAG60030.151368214339207No Hit
CTAGAGCCCCAGGACTTATGGGGAAGCAGACAGTAGGAGGAAGGGAGTGG57380.14468612591676994No Hit
ATCATGCACAGCAATACATAGTTCAGTCTGTATTCAGTAAATGCCATCAC56750.14309755395219054No Hit
TAATTAGTGTCAGCCATTCTTCCTGTCCTCTTGCAGGTTAAAACCCCCGT56630.14279496881608017No Hit
GGTTAAAACCCCCGTGGCCTTTGATTCCTCTCTTCTTTCTCCACTCCCTT56520.142517599107979No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT54710.13795360663831444No Hit
CCCTTCCTCCTACTGTCTGCTTCCCCATAAGTCCTGGGGCTCTAGTGTCT53350.1345243084290637No Hit
GTGCTTGCCAGTGCGCTCCATCATGCACAGCAATACATAGTTCAGTCTGT53090.1338687073008246No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA51940.13096893307976698No Hit
GGGTTGCATGGCAGCAGAGGATTTGAGGATGAGCCCTAGGTTTTGGCCTC51840.13071677879967503No Hit
GGATTTGAGGATGAGCCCTAGGTTTTGGCCTCAGCCACTGGGAAGATGGT50500.1273379114464427No Hit
GTATTGCTGTGCATGATGGAGCGCACTGGCAAGCACTACAGCATTTTGAA49560.1249676612135782No Hit
CTGTAGTGCTTGCCAGTGCGCTCCATCATGCACAGCAATACATAGTTCAG49480.12476593778950464No Hit
GTTTTGGCCTCAGCCACTGGGAAGATGGTGATGGCATTTACTGAATACAG49160.12395904409321033No Hit
GCACTACAGCATTTTGAATAATTAGTGTCAGCCATTCTTCCTGTCCTCTT48710.1228243498327965No Hit
ACTGAAGAGGGTTGCATGGCAGCAGAGGATTTGAGGATGAGCCCTAGGTT47810.1205549613119688No Hit
GAAAGGGCCAGTGCCAGGGAGAACTGAAAGGAGGGAACAGACACTAGAGC47500.1197732830436837No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC47400.11952112876359175No Hit
GGTTTTAACCTGCAAGAGGACAGGAAGAATGGCTGACACTAATTATTCAA47050.11863858878326987No Hit
GTTTTAACCTGCAAGAGGACAGGAAGAATGGCTGACACTAATTATTCAAA46720.11780647965896637No Hit
GAAGAGGGTTGCATGGCAGCAGAGGATTTGAGGATGAGCCCTAGGTTTTG46180.11644484654646976No Hit
GACTTGCACCGGAGCCTTTGCCACAGTCCAGGTCAGAAAGGGCCAGTGCC45480.114679766585826No Hit
CTTCCTGTCCTCTTGCAGGTTAAAACCCCCGTGGCCTTTGATTCCTCTCT45050.11359550318143054No Hit
ATTTTGAATAATTAGTGTCAGCCATTCTTCCTGTCCTCTTGCAGGTTAAA44860.11311641004925581No Hit
ATTCAAAATGCTGTAGTGCTTGCCAGTGCGCTCCATCATGCACAGCAATA44560.11235994720897992No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA43590.10991405069208784No Hit
GATGGGGCTTACTGAAGAGGGTTGCATGGCAGCAGAGGATTTGAGGATGA43520.10973754269602348No Hit
GTCCTGGGGCTCTAGTGTCTGTTCCCTCCTTTCAGTTCTCCCTGGCACTG42800.10792203187936131No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG42800.10792203187936131No Hit
GGCGTAGTAGGTATTTTGGAAGGTGAACAGATGGGGCTTACTGAAGAGGG42750.10779595473931534No Hit
GTATTTTGGAAGGTGAACAGATGGGGCTTACTGAAGAGGGTTGCATGGCA42570.1073420770351498No Hit
CCATCATGCACAGCAATACATAGTTCAGTCTGTATTCAGTAAATGCCATC42250.10653518333885552No Hit
CCATCTTCCCAGTGGCTGAGGCCAAAACCTAGGGCTCATCCTCAAATCCT41710.10517355022635891No Hit
TTTCTGACCTGGACTGTGGCAAAGGCTCCGGTGCAAGTCTCGCTGCCCAG41560.10479531880622096No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA41360.10429101024603703No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT41320.10419014853400024No Hit
GGCTTACTGAAGAGGGTTGCATGGCAGCAGAGGATTTGAGGATGAGCCCT40680.10257636114141165No Hit
GAAGAGAGGAATCAAAGGCCACGGGGGTTTTAACCTGCAAGAGGACAGGA40620.10242506857335648No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)