FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_E6a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_E6a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1098792
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAGGATGGGTGGGAGGCACCAGCTCCAGGCCAGCAGGGCAGCGAGGA76530.6964921477404277No Hit
GTCCTAGACTCTTTATTTATTTGCTTATTTATTTTTTGAGGTAGAGTCTC70500.6416136994080772No Hit
GGAATAAGCAGGTCAGAAGCTCATCCTGTGATGGAGCAGGCTAGGATGGG58770.5348601009108185No Hit
ATTCTGGATATGACCATTTTCTGCACAGCCACCATGTGCCTGATAGAGAA42060.38278400279579755No Hit
GTCTTGGTGAGAGCAGAGAGCACTCCATTCTGGATATGACCATTTTCTGC40530.3688596203831116No Hit
GTTCATAGGATGTACGACTCAAGGCCGCAGCAGGGGAGGATAGGAGCCTA35990.327541518321939No Hit
GGTCAGAAGCTCATCCTGTGATGGAGCAGGCTAGGATGGGTGGGAGGCAC33400.3039701781592877No Hit
GCCACCATGTGCCTGATAGAGAAGCACTCTAGGCTCCTATCCTCCCCTGC32950.29987477156732123No Hit
CTCTAGGCTCCTATCCTCCCCTGCTGCGGCCTTGAGTCGTACATCCTATG31590.28749754275604483No Hit
GCACACACCTGTCCCCAATGCCTGAGACCTGCACACACCTGTCCCTGCTA27430.2496377840391994No Hit
GGACAGATGTGTGCATGCAGGTCTCAGGCACTGAGAACAGGTGTGTGCAC26890.24472329612883967No Hit
CACCTGTCCCTGCTATCAAAGACCTGCATACACCTCTCCTCACTGCCTGA26700.24299412445667606No Hit
GGGGAGGATGGACCCCTCACCCCCTCTGCTGCAGAGGCCAGGTCTTGGTG26340.2397177991831029No Hit
GTGTATGCACATCTCTGATAGCAGGGACAGGTTTGTGCATGTCTCAGGCA26290.23926275400621774No Hit
GTACATCACATAGTGACAGTGGGAGCAAGAGAGAGAGATAAGGGGTAGGT25140.22879671493785902No Hit
GGCTGGCACTGCACATCGGGCTGGGAGGATGAACCCTGACCCAGGGCAAG24950.22706754326569542No Hit
CTGTTACCCAGGCTGGAGTGCAGTGGCATGATCTTGGCTCACGGCAACCT24170.21996883850628693No Hit
GGATGGACCCCTCACCCCCTCTGCTGCAGAGGCCAGGTCTTGGTGAGAGC24060.21896773911713954No Hit
CTGCTGCAGAGGCCAGGTCTTGGTGAGAGCAGAGAGCACTCCATTCTGGA24050.2188767300817625No Hit
GATGTGTGCATGCAGGTCTCAGGCACTGAGAACAGGTGTGTGCACGTCTC23050.2097758265440593No Hit
CATAGTGACAGTGGGAGCAAGAGAGAGAGATAAGGGGTAGGTCCTAGACT22930.2086837181195349No Hit
GGCCAGGTCTTGGTGAGAGCAGAGAGCACTCCATTCTGGATATGACCATT22920.20859270908415786No Hit
CCTATGAACTCAGCTCCCCTCTTCCTGCCCTTGCCCTGGGTCAGGGTTCA22790.20740959162425646No Hit
GTGATGGAGCAGGCTAGGATGGGTGGGAGGCACCAGCTCCAGGCCAGCAG22750.2070455554827483No Hit
GCACACACCTGTCCCTGCTATCAAAGACCTGCATACACCTCTCCTCACTG22560.2053163838105847No Hit
CTTTATTTATTTGCTTATTTATTTTTTGAGGTAGAGTCTCACTCTGTTAC21440.1951233718483571No Hit
GTCTAGGACCTACCCCTTATCTCTCTCTCTTGCTCCCACTGTCACTATGT20870.18993585683186626No Hit
GCATACACCTCTCCTCACTGCCTGAGACATGCACAAACCTGTCCCTGCTA20420.1858404502398998No Hit
GTCTCACTCTGTTACCCAGGCTGGAGTGCAGTGGCATGATCTTGGCTCAC20200.18383825146160512No Hit
CCTGTAGTCTCAACTACTTGGGAAGCTGAGGGAGGAGAATCACTTCAACC19760.1798338539050157No Hit
CTTCCTGCCCTTGCCCTGGGTCAGGGTTCATCCTCCCAGCCCGATGTGCA19560.17801367319747505No Hit
TCCTGTGATGGAGCAGGCTAGGATGGGTGGGAGGCACCAGCTCCAGGCCA19170.17446432081777077No Hit
CATGGTGGCTGTGCAGAAAATGGTCATATCCAGAATGGAGTGCTCTCTGC18710.1702779051904273No Hit
ACCCTGTCTCTACTAAAAATACAAAAATTAGGCGGGTGTGGTGGTCCACG18510.16845772448288665No Hit
CTCCTATCCTCCCCTGCTGCGGCCTTGAGTCGTACATCCTATGAACTCAG18080.16454433596167428No Hit
GAGCAGAGAGCACTCCATTCTGGATATGACCATTTTCTGCACAGCCACCA18010.16390727271403505No Hit
GGGTAGGGTCCGCCATCAGGAGCTTAGTGGAGAGACCATTGTGCAACATG17970.16354323657252692No Hit
ACTCAAGGCCGCAGCAGGGGAGGATAGGAGCCTAGAGTGCTTCTCTATCA17960.16345222753714989No Hit
GGATAGGAGCCTAGAGTGCTTCTCTATCAGGCACATGGTGGCTGTGCAGA17830.16226911007724845No Hit
CCCTGACCCAGGGCAAGGGCAGGAAGAGGGGAGCTGAGTTCATAGGATGT17410.15844673059141312No Hit
CCCCCTCTGCTGCAGAGGCCAGGTCTTGGTGAGAGCAGAGAGCACTCCAT17220.1567175589192495No Hit
AGCCTAGAGTGCTTCTCTATCAGGCACATGGTGGCTGTGCAGAAAATGGT16870.15353224268105337No Hit
CCATTCTGGATATGACCATTTTCTGCACAGCCACCATGTGCCTGATAGAG16840.15325921557492228No Hit
GGGTAACAGAGTGAGACTCTACCTCAAAAAATAAATAAGCAAATAAATAA16790.15280417039803712No Hit
AGCCAGTACATCACATAGTGACAGTGGGAGCAAGAGAGAGAGATAAGGGG16710.15207609811502085No Hit
GATGAACCCTGACCCAGGGCAAGGGCAGGAAGAGGGGAGCTGAGTTCATA16540.1505289445136113No Hit
GGATATGACCATTTTCTGCACAGCCACCATGTGCCTGATAGAGAAGCACT16450.149709863195218No Hit
GGCAGGAAGAGGGGAGCTGAGTTCATAGGATGTACGACTCAAGGCCGCAG16440.149618854159841No Hit
ATCTTGGCTCACGGCAACCTCTGCCTCCCAGGTTGAAGTGATTCTCCTCC16410.14934582705370988No Hit
GCCTGATAGAGAAGCACTCTAGGCTCCTATCCTCCCCTGCTGCGGCCTTG16380.1490727999475788No Hit
TTTCTGCACAGCCACCATGTGCCTGATAGAGAAGCACTCTAGGCTCCTAT16040.1459784927447597No Hit
GTCCACGCCTGTAGTCTCAACTACTTGGGAAGCTGAGGGAGGAGAATCAC15960.14525042046174344No Hit
ATGTACGACTCAAGGCCGCAGCAGGGGAGGATAGGAGCCTAGAGTGCTTC15720.14306620361269468No Hit
ACCCTGACCCAGGGCAAGGGCAGGAAGAGGGGAGCTGAGTTCATAGGATG15650.14242914036505544No Hit
CACCTGTCCCCAATGCCTGAGACCTGCACACACCTGTCCCTGCTATCAAA15590.14188308615279327No Hit
GCCTTGAGTCGTACATCCTATGAACTCAGCTCCCCTCTTCCTGCCCTTGC15580.1417920771174162No Hit
AAGCAGGGGAGGATGGACCCCTCACCCCCTCTGCTGCAGAGGCCAGGTCT15360.13978987833912151No Hit
CTCTGCTGCAGAGGCCAGGTCTTGGTGAGAGCAGAGAGCACTCCATTCTG15300.13924382412685932No Hit
GGTGTGTGCTGGTCTCAGGCAGGGGGACAGGTGCGTGCAGGTCTCAGGCA15280.13906180605610524No Hit
GGTGTATGCAGGTCTTTGATAGCAGGGACAGGTGTGTGCAGGTCTCAGGC15090.13733263438394164No Hit
GTCTCAGGCAGTGAGGAGAGGTGTATGCAGGTCTTTGATAGCAGGGACAG14900.13560346271177803No Hit
GAACAGGTGTGTGCACGTCTCAGGAAGAGGGACAAGTGTATGCACATCTC14790.13460236332263067No Hit
GAGTTCATAGGATGTACGACTCAAGGCCGCAGCAGGGGAGGATAGGAGCC14670.13351025489810628No Hit
CCATGTGCCTGATAGAGAAGCACTCTAGGCTCCTATCCTCCCCTGCTGCG14170.12895980312925467No Hit
GTTTGAGACCAGCCTGGCCAACATGGCGAAACCCTGTCTCTACTAAAAAT14040.12777668566935327No Hit
GCCTGAGACATGCACAAACCTGTCCCTGCTATCAGAGATGTGCATACACT14030.12768567663397623No Hit
GGGCAAGGGCAGGAAGAGGGGAGCTGAGTTCATAGGATGTACGACTCAAG13980.12723063145709104No Hit
ATCCAGAATGGAGTGCTCTCTGCTCTCACCAAGACCTGGCCTCTGCAGCA13960.127048613386337No Hit
CGCCTGTAGTCTCAACTACTTGGGAAGCTGAGGGAGGAGAATCACTTCAA13840.1259565049618126No Hit
CCTCACCCCCTCTGCTGCAGAGGCCAGGTCTTGGTGAGAGCAGAGAGCAC13760.12522843267879635No Hit
GATAAGGGGTAGGTCCTAGACTCTTTATTTATTTGCTTATTTATTTTTTG13750.12513742364341932No Hit
TCTCAGGAAGAGGGACAAGTGTATGCACATCTCTGATAGCAGGGACAGGT13730.12495540557266527No Hit
GTGTGGTGGTCCACGCCTGTAGTCTCAACTACTTGGGAAGCTGAGGGAGG13660.12431834232502603No Hit
GAAGAGGGGAGCTGAGTTCATAGGATGTACGACTCAAGGCCGCAGCAGGG13610.12386329714814087No Hit
GGCTGGGAGGATGAACCCTGACCCAGGGCAAGGGCAGGAAGAGGGGAGCT13590.1236812790773868No Hit
ACATCACATAGTGACAGTGGGAGCAAGAGAGAGAGATAAGGGGTAGGTCC12800.11649156528260125No Hit
CCCCTCACCCCCTCTGCTGCAGAGGCCAGGTCTTGGTGAGAGCAGAGAGC12720.115763492999585No Hit
AGCTCATCCTGTGATGGAGCAGGCTAGGATGGGTGGGAGGCACCAGCTCC12670.11530844782269983No Hit
GCTCCAGGCCAGCAGGGCAGCGAGGAGGCTGGCACTGCACATCGGGCTGG12390.11276019483214295No Hit
ATCCTCCCCTGCTGCGGCCTTGAGTCGTACATCCTATGAACTCAGCTCCC12360.11248716772601183No Hit
GCACTGCACATCGGGCTGGGAGGATGAACCCTGACCCAGGGCAAGGGCAG12350.11239615869063481No Hit
GTCGTACATCCTATGAACTCAGCTCCCCTCTTCCTGCCCTTGCCCTGGGT12340.11230514965525779No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC12220.11121304123073339No Hit
CTATCAGGCACATGGTGGCTGTGCAGAAAATGGTCATATCCAGAATGGAG12210.11112203219535637No Hit
GTGCCAGCCTCCTCGCTGCCCTGCTGGCCTGGAGCTGGTGCCTCCCACCC12170.11075799605384823No Hit
GAGTGAGACTCTACCTCAAAAAATAAATAAGCAAATAAATAAAGAGTCTA12090.11002992377083197No Hit
AGCGAAAGCAGGGGAGGATGGACCCCTCACCCCCTCTGCTGCAGAGGCCA11970.10893781534630759No Hit
GGATGTACGACTCAAGGCCGCAGCAGGGGAGGATAGGAGCCTAGAGTGCT11820.1075726798156521No Hit
AATTAGGCGGGTGTGGTGGTCCACGCCTGTAGTCTCAACTACTTGGGAAG11820.1075726798156521No Hit
ATAGAGAAGCACTCTAGGCTCCTATCCTCCCCTGCTGCGGCCTTGAGTCG11810.10748167078027505No Hit
CCCCTGCTGCGGCCTTGAGTCGTACATCCTATGAACTCAGCTCCCCTCTT11740.10684460753263585No Hit
CTCCAGGCCAGCAGGGCAGCGAGGAGGCTGGCACTGCACATCGGGCTGGG11720.10666258946188177No Hit
GAGAACAGGTGTGTGCACGTCTCAGGAAGAGGGACAAGTGTATGCACATC11680.10629855332037363No Hit
GTGGTGGTCCACGCCTGTAGTCTCAACTACTTGGGAAGCTGAGGGAGGAG11630.10584350814348847No Hit
CCTGTCCCCAATGCCTGAGACCTGCACACACCTGTCCCTGCTATCAAAGA11440.10411433647132487No Hit
GCACATCGGGCTGGGAGGATGAACCCTGACCCAGGGCAAGGGCAGGAAGA11250.10238516479916125No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)