FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_E4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_E4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5842899
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTGATCTGCCTATCTGCGTACATACATCGATAGTATTCTGAAAGCAA401090.6864571850377698No Hit
AGTAATAACAGCACGTATTTATTAAGGGACTACTACATTCCACATCCTGT332820.5696145012946484No Hit
GATATATCTCCATATATATTGATCTGCCTATCTGCGTACATACATCGATA313970.5373531187172669No Hit
GTTATTACTATGGTGCAAAAATCATCTTGCTGGGTGGAATACAGGTACTA302270.5173288122899267No Hit
TTATGATACTGGACAGCAAGCAAACCTGCCTTTGTGCTCTGGAGAGAGAC292870.5012409079807815No Hit
GTACAGGGAGGCACTAGTACCTGTATTCCACCCAGCAAGATGATTTTTGC274490.4697839206188572No Hit
CCATATATATTGATCTGCCTATCTGCGTACATACATCGATAGTATTCTGA253820.43440764593055603No Hit
GCCTATCTGCGTACATACATCGATAGTATTCTGAAAGCAATGGGGATCTG203900.34897060517390427No Hit
TCCCTGTACCGACCCTGTCCCTGCATGTCCTTCCAGCACAGGCATTGGTG201590.34501708826389094No Hit
CTTCTAAGACTGTTTGCTAAAAATCACCTTGAAGGGGTAATTCAGAACAA183150.3134574121510572No Hit
GTCTTAATGCTTTAGCTGTTTTAACTCTTTTGGTCCCTCATACGTGAGAG177390.30359929206375125No Hit
ATACTGGACAGCAAGCAAACCTGCCTTTGTGCTCTGGAGAGAGACATGAT177120.3031371926846588No Hit
GTAATAACAGCACGTATTTATTAAGGGACTACTACATTCCACATCCTGTC167440.2865700742046029No Hit
TTCTAAGACTGTTTGCTAAAAATCACCTTGAAGGGGTAATTCAGAACAAA165900.283934396264594No Hit
GTTGATTACCTGTTAGAGCCTCTCACGTATGAGGGACCAAAAGAGTTAAA160970.2754968038982019No Hit
CATCGATAGTATTCTGAAAGCAATGGGGATCTGTTGATTACCTGTTAGAG136200.23310346456442257No Hit
GCGTACATACATCGATAGTATTCTGAAAGCAATGGGGATCTGTTGATTAC134570.2303137534980495No Hit
ATAGTAATAACAGCACGTATTTATTAAGGGACTACTACATTCCACATCCT129770.2220986534252945No Hit
GTTAGAGCCTCTCACGTATGAGGGACCAAAAGAGTTAAAACAGCTAAAGC128480.21989084528074165No Hit
GTCTTAGAAGACAGAGATCATGTCTCTCTCCAGAGCACAAAGGCAGGTTT125090.21408893085435843No Hit
ATAGTATTCTGAAAGCAATGGGGATCTGTTGATTACCTGTTAGAGCCTCT121830.20850950872161236No Hit
ATCTTTATGATACTGGACAGCAAGCAAACCTGCCTTTGTGCTCTGGAGAG120820.20678091474797014No Hit
ACTACATTCCACATCCTGTCTTAATGCTTTAGCTGTTTTAACTCTTTTGG120320.20592517515705816No Hit
ATATATCTCCATATATATTGATCTGCCTATCTGCGTACATACATCGATAG117870.20173205116158946No Hit
GTATTCCACCCAGCAAGATGATTTTTGCACCATAGTAATAACAGCACGTA115590.19782987862703086No Hit
AAACAAGGGCTTGAATTGGTTGGTTTGAACTCGTCTCAAAATACATGCAG114240.19551938173156852No Hit
CGTATGAGGGACCAAAAGAGTTAAAACAGCTAAAGCATTAAGACAGGATG110420.188981531257001No Hit
CTTTATGATACTGGACAGCAAGCAAACCTGCCTTTGTGCTCTGGAGAGAG110040.18833116916790793No Hit
GACAGATATATCTCCATATATATTGATCTGCCTATCTGCGTACATACATC108750.18612336102335503No Hit
GCCTTAGAGGCAGACAGGATCTTTATGATACTGGACAGCAAGCAAACCTG106040.1814852524406121No Hit
GTACCGACCCTGTCCCTGCATGTCCTTCCAGCACAGGCATTGGTGAAATT106020.18145102285697562No Hit
CATATATATTGATCTGCCTATCTGCGTACATACATCGATAGTATTCTGAA105340.18028721701333533No Hit
TTCCACATCCTGTCTTAATGCTTTAGCTGTTTTAACTCTTTTGGTCCCTC100880.1726540198624005No Hit
TATTACTATGGTGCAAAAATCATCTTGCTGGGTGGAATACAGGTACTAGT100280.17162713235330612No Hit
GCATTAAGACAGGATGTGGAATGTAGTAGTCCCTTAATAAATACGTGCTG98490.16856358461784124No Hit
CTGTTATTACTATGGTGCAAAAATCATCTTGCTGGGTGGAATACAGGTAC88810.15199646613778536No Hit
GGAGAGAGACATGATCTCTGTCTTCTAAGACTGTTTGCTAAAAATCACCT88780.15194512176233066No Hit
GTAGTAGTCCCTTAATAAATACGTGCTGTTATTACTATGGTGCAAAAATC86610.14823121193777267No Hit
ATGTTACGTAGACAGATATATCTCCATATATATTGATCTGCCTATCTGCG83230.1424464123032077No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC82840.14177893542229636No Hit
TCTCCATATATATTGATCTGCCTATCTGCGTACATACATCGATAGTATTC82640.14143663958593158No Hit
TACGTAGACAGATATATCTCCATATATATTGATCTGCCTATCTGCGTACA82340.14092319583138438No Hit
TGCAGGGACAGGGTCGGTACAGGGAGGCACTAGTACCTGTATTCCACCCA81510.13950266811047052No Hit
GTAGAAACAAGGGCTTGAATTGGTTGGTTTGAACTCGTCTCAAAATACAT80550.13785964809591952No Hit
ATGTAGTAGTCCCTTAATAAATACGTGCTGTTATTACTATGGTGCAAAAA80010.13693544933773455No Hit
GGTGATGCCACTCGCACACTGAGTTTGTGCCAAAGCCGAAGAGCTTAGCA79160.1354806920331842No Hit
GACTACTACATTCCACATCCTGTCTTAATGCTTTAGCTGTTTTAACTCTT76880.1315785194986256No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG76840.13151006033135262No Hit
AACCTAACCATGACTTGCCTTAGAGGCAGACAGGATCTTTATGATACTGG76200.1304147136549853No Hit
CCTGTATTCCACCCAGCAAGATGATTTTTGCACCATAGTAATAACAGCAC75390.1290284155177079No Hit
GGCTTGAATTGGTTGGTTTGAACTCGTCTCAAAATACATGCAGAGAACCC74680.1278132652986129No Hit
CTACTACATTCCACATCCTGTCTTAATGCTTTAGCTGTTTTAACTCTTTT72380.12387686318041781No Hit
CCATAGTAATAACAGCACGTATTTATTAAGGGACTACTACATTCCACATC72260.12367148567859892No Hit
TTACTATGGTGCAAAAATCATCTTGCTGGGTGGAATACAGGTACTAGTGC72150.12348322296859829No Hit
TATTAAGGGACTACTACATTCCACATCCTGTCTTAATGCTTTAGCTGTTT72110.12341476380132535No Hit
GTCCCTGCATGTCCTTCCAGCACAGGCATTGGTGAAATTTTGTTCTGAAT71100.12168616982768314No Hit
TAGTAATAACAGCACGTATTTATTAAGGGACTACTACATTCCACATCCTG70790.12115561128131772No Hit
AACCATGACTTGCCTTAGAGGCAGACAGGATCTTTATGATACTGGACAGC70750.12108715211404475No Hit
GAATGTAGTAGTCCCTTAATAAATACGTGCTGTTATTACTATGGTGCAAA69370.11872531084312771No Hit
TACATCGATAGTATTCTGAAAGCAATGGGGATCTGTTGATTACCTGTTAG69310.11862262209221826No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA68740.11764707895857861No Hit
TACATACATCGATAGTATTCTGAAAGCAATGGGGATCTGTTGATTACCTG67520.11555907435675339No Hit
GGGTAATTCAGAACAAAATTTCACCAATGCCTGTGCTGGAAGGACATGCA66820.1143610389294766No Hit
GCTTAGCACAGGCAGACCCAGTCTTTCCTAGCAGCCTCTGCGCAAACCTA66630.11403585788493006No Hit
GTGGAATACAGGTACTAGTGCCTCCCTGTACCGACCCTGTCCCTGCATGT66490.11379625079947471No Hit
CACTGAGTTTGTGCCAAAGCCGAAGAGCTTAGCACAGGCAGACCCAGTCT64830.11095519535764696No Hit
GCTCTGGAGAGAGACATGATCTCTGTCTTCTAAGACTGTTTGCTAAAAAT64310.1100652261830985No Hit
GAATACAGGTACTAGTGCCTCCCTGTACCGACCCTGTCCCTGCATGTCCT61780.10573518385308389No Hit
CATTAAGACAGGATGTGGAATGTAGTAGTCCCTTAATAAATACGTGCTGT61740.10566672468581093No Hit
GGCACGGGTGATGCCACTCGCACACTGAGTTTGTGCCAAAGCCGAAGAGC61400.10508482176399078No Hit
GTTTTAACTCTTTTGGTCCCTCATACGTGAGAGGCTCTAACAGGTAATCA60790.10404081946307818No Hit
CCCCAAATGTTACGTAGACAGATATATCTCCATATATATTGATCTGCCTA60270.10315085028852972No Hit
TATTTATTAAGGGACTACTACATTCCACATCCTGTCTTAATGCTTTAGCT60020.10272298049307373No Hit
GTATTATATGGCATGAATATGGCACACTTTGTTTATCCATTCTCTAGTTG60010.1027058657012555No Hit
GTACTAGTGCCTCCCTGTACCGACCCTGTCCCTGCATGTCCTTCCAGCAC59010.10099438651943154No Hit
GGTCGGTACAGGGAGGCACTAGTACCTGTATTCCACCCAGCAAGATGATT58540.10018999130397428No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)