FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_E3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_E3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3240604
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATAGATAGATAGATCTTTACTCTGGACAGTTAGGTGGGAACAGTTTT68290.21073232027115932No Hit
ATACAATCTGTACCCCGATGATTAAATGCAATTCTAGTTCTCGAACTCTT55050.16987573921404775No Hit
GTGCAGGGGTTGATCCCCCGGACCCTGGGATCATGACCTGAGCCGAAAAG49640.15318132051926123No Hit
ACAGTGGACTGTCCCTCGTGGAGCTTGGATGGACTGAGCATTCCTGAGCC48530.1497560331345638No Hit
ATATTGTACCTTGTCATTTGCAAATAGTGACAGTTTTACTTTTTCCTACC46890.14469524816978563No Hit
TGGTATTACTGTCCAACTGAAAGGAAACATACTCTTAGAAGTGTATTTTG46760.14429408838599225No Hit
CTATACAATCTGTACCCCGATGATTAAATGCAATTCTAGTTCTCGAACTC44880.13849270074344167No Hit
CTATATTACCTGGCAAACAATATTTCGTGTTTCTAACTTATCTTTTCCTT43240.13343191577866348No Hit
ATATTAATATGGCTACTCAAAGCAACTCACTAGTCAGCTAGACATTAGAT43150.13315418977449883No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT41070.12673563323380457No Hit
GTCAATGACAGTGGACTGTCCCTCGTGGAGCTTGGATGGACTGAGCATTC38460.11868157911302955No Hit
GTGTGAGGCGTGACTGATTTGCAATTCAAAAAGCTCCAACAAAGGCAGAA36190.11167671211909878No Hit
CTTCAGGACCCCCCGGTTGGGGGGAACGTGCCCAGTAAAGAACTGAAATG35210.10865258451819475No Hit
ATTCCATAGTGTCTGAGAATGAGGGCCATACCCTGCTCCCTTAACCCAAA33660.10386952555758124No Hit
TATAAAAACCACTCAAGCTTTAGGTACTGCTATTAATGCTAGAAATAACT32490.10025908750344072No Hit
GTGTAAGGGCTCTTTATTTCTCATTGCGATGATGTACTCGTTAGGAATAA32480.10022822905853353No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)