FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_E10a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_E10a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1670763
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG321121.9219961179413239No Hit
GTACTATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAG133410.7984974529601147No Hit
GTATTAATCTCCTTTAAGTATTGCTTCTGAGTTTATATTTGGTTTGCTTG102490.6134323060781213No Hit
GTTAAATGATGATCTAAATCTTCAGAAGGTCACAGTACCTTTTGGAAGAC87920.5262266401638054No Hit
GTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATGAACAAGGAATAACC87530.5238923773150351No Hit
CCTTTAAGTATTGCTTCTGAGTTTATATTTGGTTTGCTTGCTTGCAGCTT82980.4966593107460483No Hit
ATTCAAGCCTTTAAAGTTAGCAGTTAAATGATGATCTAAATCTTCAGAAG81760.48935725773194644No Hit
CCTTTAAAGTTAGCAGTTAAATGATGATCTAAATCTTCAGAAGGTCACAG79690.4769677087653964No Hit
GCATTATTCAAGCCTTTAAAGTTAGCAGTTAAATGATGATCTAAATCTTC71130.42573363187956637No Hit
GTGCATAATCAGTGAAATCTTGGAACACTGAAAAAATTAAAAAAGAAAAA69770.41759363835564944No Hit
ATTTTAGAGTAACAAGATATTAGACCCAGGTGACAGTCCTGAGATCTAAA62970.3768936707360649No Hit
ATATATGCCAAACAAACTTCTATTTTCTAAAGACTCTGGGATTGCTAAGT59950.3588180968814847No Hit
ATACTGTGTTTTATTTTTCCTATGTAGTTTTCCATTTTCAGCTGTTATGA58900.3525335430578724No Hit
GTTTTATAGCATTATTCAAGCCTTTAAAGTTAGCAGTTAAATGATGATCT58290.3488825165508214No Hit
ATATTAGACCCAGGTGACAGTCCTGAGATCTAAATTGTTGTGTTTCTATG56720.339485612262182No Hit
GTATTAAGGTGGGCACGTATTGCATGGAGCACTGGGTATGGTGCATAATC55350.33128576584470687No Hit
CTATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAA52890.3165619540293866No Hit
GTATAATTTAGTATTTCAGACCCAATCTTCTCCCCAACCCTGCAAAGGAA52090.31177372254472957No Hit
GCATGATACTGTGTTTTATTTTTCCTATGTAGTTTTCCATTTTCAGCTGT51880.3105168117800071No Hit
GGGTATGGTGCATAATCAGTGAAATCTTGGAACACTGAAAAAATTAAAAA45970.2751437516871035No Hit
GTTTTATTGCATAGAAACACAACAATTTAGATCTCAGGACTGTCACCTGG43710.2616169977429474No Hit
TCCCAGAGCAGTGAGAAATAATGTTAACTTTGTCACAGTAAATGCGATTC39770.2380349576810116No Hit
GTTATGAAGCTTATCAATTGTGTGGACAGACATCTGCATTGTTGCCAGTT39740.237855399000337No Hit
CACATATGGAAGCACAAATTTTTTTTTCTCTTGACATTGTTTTTAGTTTC39130.23420437249328602No Hit
CTGTATAATTTAGTATTTCAGACCCAATCTTCTCCCCAACCCTGCAAAGG37680.2255257029273452No Hit
TGTGTATACAGATTTTAATAGGCCTTCAGGAATTTTACATATCTATCATC37450.2241490863755063No Hit
ACCTATGCCTGCAAGGCCATCAGCGGGAGGAAACGAGGCAGCCGGAATCC35210.21074203821846663No Hit
CTGTAAGAGTTTTATAGCATTATTCAAGCCTTTAAAGTTAGCAGTTAAAT35180.210562479537792No Hit
GTAAAGGTATCTTTGATAACCTAAATTTGAAAATGAAAAGCTGCAAGCAA34890.20882674562460382No Hit
TTATAGCATTATTCAAGCCTTTAAAGTTAGCAGTTAAATGATGATCTAAA34750.20798880511478887No Hit
CTTAACAACTGAGCCACCCAGGGGCCCTGCTGCATTATTTTAGAGTAACA34130.2042779257141797No Hit
ATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATGAA33750.2020035157589676No Hit
CATATGGAAGCACAAATTTTTTTTTCTCTTGACATTGTTTTTAGTTTCAT33180.19859190082614947No Hit
ATCTAAATCTTCAGAAGGTCACAGTACCTTTTGGAAGACATACAGACATA33120.1982327834648002No Hit
ACTTAAAGGAGATTAATACATGTGGCAGATAAAAAAAATCTGGTTTCTAA32970.19733499006142705No Hit
ACACAGTATCATGCAAAAACATGAATGAAACTAAAAACAATGTCAAGAGA32900.19691601980651954No Hit
ACTGTAAGAGTTTTATAGCATTATTCAAGCCTTTAAAGTTAGCAGTTAAA32900.19691601980651954No Hit
GTTTTAAATGTATGTCTGTATGTCTTCCAAAAGGTACTGTGACCTTCTGA32700.19571896193535526No Hit
CTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATGAACAAGGAATAA32650.19541969746756424No Hit
GTATCATGCAAAAACATGAATGAAACTAAAAACAATGTCAAGAGAAAAAA32000.1915292593862804No Hit
TCCCAGGACCCTGGGATCATGACCTGAGCCAAAGGCAGACACTTAACAAC31870.19075117177002365No Hit
TTTAATGACTGAGCCACCAAGGCGCTCCTGTAGTTCCACTTAAATAAGAT31550.18883587917616082No Hit
CCTGTAGTTCCACTTAAATAAGATTCATAATTTTTGGCTGGCCAGTAAAG31260.18710014526297267No Hit
CTCCTGTAGTTCCACTTAAATAAGATTCATAATTTTTGGCTGGCCAGTAA30640.18338926586236348No Hit
GTGTTTTATTTTTCCTATGTAGTTTTCCATTTTCAGCTGTTATGAAGCTT30070.17997765092954535No Hit
CAATTGATAAGCTTCATAACAGCTGAAAATGGAAAACTACATAGGAAAAA29820.17848132859059004No Hit
CATAAATACTGAGGTCTGGTTGGAATCGCATTTACTGTGACAAAGTTAAC29460.1763266244224944No Hit
GTCTGAAGTAAGCAATTACATTAGAAAATTCTGGAACAACATTCTCATAA29380.1758478012740287No Hit
GTATTGCATGGAGCACTGGGTATGGTGCATAATCAGTGAAATCTTGGAAC29320.1754886839126794No Hit
GTATTTATGAGAATGTTGTTCCAGAATTTTCTAATGTAATTGCTTACTTC29050.17387265578660768No Hit
AAATATATGTGGTTAGAAACCAGATTTTTTTTATCTGCCACATGTATTAA28600.1711792755764881No Hit
TATCAATTGTGTGGACAGACATCTGCATTGTTGCCAGTTTCTGGCTATAC28410.17004207059888204No Hit
GTATTTGAAATTAGTAGGCAATTTCAACCCACCTACAGATGCTTTCTGCA28250.16908442430195067No Hit
GTCCTGAGATCTAAATTGTTGTGTTTCTATGCAATAAAACCGTGTGGAAG27920.16710927881452967No Hit
ATTTAGTATTTCAGACCCAATCTTCTCCCCAACCCTGCAAAGGAAACCAA27770.16621148541115646No Hit
CAATAAAACCGTGTGGAAGACTGTGTTCAGTGGCATACACTAAATGCAGA27740.1660319267304818No Hit
GTTTTTAGTTTCATTCATGTTTTTGCATGATACTGTGTTTTATTTTTCCT27680.16567280936913253No Hit
GATTACAAGTAGGCAGAGAGGCAGACAGAGAGAGGAGGAAGCAGGCTCCC27440.16423633992373546No Hit
CTTCATAACAGCTGAAAATGGAAAACTACATAGGAAAAATAAAACACAGT26740.16004663737466054No Hit
GTGAGAGAGGGAACCCAAGCAGGGGGAATGGGAGAGGGAGAAACATGCTT26580.15908899107772914No Hit
TATATGTGGTTAGAAACCAGATTTTTTTTATCTGCCACATGTATTAATCT26340.15765252163233207No Hit
CCTCTATGCTAAGCCAGGCACCCGTCGCAACATGCAAACACAGTACCTTC25990.1555576703577946No Hit
CATACAGACATACATTTAAAACTTGTTTGGCTGGTTATTCCTTGTTCATT25760.15418105380595573No Hit
ATGTATGTCTGTATGTCTTCCAAAAGGTACTGTGACCTTCTGAAGATTTA25670.1536423777639318No Hit
GTGTTACGCAGTTAAAGCATCAGTCACAAGGGAAAATGAGCTTTTTCCGC25350.151727085170069No Hit
GCATTTAGTGTATGCCACTGAACACAGTCTTCCACACGGTTTTATTGCAT25190.1507694388731376No Hit
ACTCTACTGTAAGAGTTTTATAGCATTATTCAAGCCTTTAAAGTTAGCAG24870.1488541462792748No Hit
GTCATTAAATGTCTGTCTTTGGCTCAGGTCACGTTCCCGGGGTCCTGGAA24860.14879429338571656No Hit
GGAATAACCAGCCAAACAAGTTTTAAATGTATGTCTGTATGTCTTCCAAA24500.14663958921762094No Hit
TCATTTAACTGCTAACTTTAAAGGCTTGAATAATGCTATAAAACTCTTAC24460.14640017764338809No Hit
GTATGGTGCATAATCAGTGAAATCTTGGAACACTGAAAAAATTAAAAAAG24440.14628047185627166No Hit
GTCTGTATGTCTTCCAAAAGGTACTGTGACCTTCTGAAGATTTAGATCAT23790.14239003377498782No Hit
GTTTTATTTTTCCTATGTAGTTTTCCATTTTCAGCTGTTATGAAGCTTAT23700.14185135773296392No Hit
GTGTATGTGTATACAGATTTTAATAGGCCTTCAGGAATTTTACATATCTA23680.1417316519458475No Hit
GATTAATACATGTGGCAGATAAAAAAAATCTGGTTTCTAACCACATATAT23580.14113312301026537No Hit
GACATACAGACATACATTTAAAACTTGTTTGGCTGGTTATTCCTTGTTCA23190.13879886016149506No Hit
AAATTATATATGCCAAACAAACTTCTATTTTCTAAAGACTCTGGGATTGC22990.13760180229033084No Hit
CACACATATGGAAGCACAAATTTTTTTTTCTCTTGACATTGTTTTTAGTT22800.13646459731272478No Hit
TCACAGTACCTTTTGGAAGACATACAGACATACATTTAAAACTTGTTTGG22490.1346091576124202No Hit
GATTTTAATAGGCCTTCAGGAATTTTACATATCTATCATCAGGATGTATT22310.13353180552837235No Hit
ATTGATAAGCTTCATAACAGCTGAAAATGGAAAACTACATAGGAAAAATA22250.13317268816702307No Hit
CCGTACATCGTTCACATCTGCTACTCACCACCAACTCGACGTCTCCTCCT22080.13215518897653347No Hit
GTTTATATTTGGTTTGCTTGCTTGCAGCTTTTCATTTTCAAATTTAGGTT21910.13113768978604384No Hit
ATGCTAAGCCAGGCACCCGTCGCAACATGCAAACACAGTACCTTCTAATT21890.13101798399892745No Hit
CTCTTACAGTAGAGTCATTAACTAAAAAGAACAGAGGGTTGCATTTTTCT21720.13000048480843782No Hit
GTTAGAAACCAGATTTTTTTTATCTGCCACATGTATTAATCTCCTTTAAG21650.12958151455353034No Hit
GACATACATTTAAAACTTGTTTGGCTGGTTATTCCTTGTTCATTCCTGGA21220.1270078401305272No Hit
AGTAAGCACCGTACATCGTTCACATCTGCTACTCACCACCAACTCGACGT21050.12599034094003758No Hit
CACACACACATATGGAAGCACAAATTTTTTTTTCTCTTGACATTGTTTTT20830.12467357728175689No Hit
CAGTAAAGGTATCTTTGATAACCTAAATTTGAAAATGAAAAGCTGCAAGC20780.12437431281396584No Hit
GTTAATGACTCTACTGTAAGAGTTTTATAGCATTATTCAAGCCTTTAAAG20320.12162107971028806No Hit
GCATTACTTGTATAGCCAGAAACTGGCAACAATGCAGATGTCTGTCCACA20270.12132181524249698No Hit
CAGTGAGAGAGGGAACCCAAGCAGGGGGAATGGGAGAGGGAGAAACATGC20210.1209626978811477No Hit
CTCTGGGATTGCTAAGTCTGAAGTAAGCAATTACATTAGAAAATTCTGGA20130.12048387473268202No Hit
CTGTAGTTCCACTTAAATAAGATTCATAATTTTTGGCTGGCCAGTAAAGG19960.11946637554219239No Hit
GTATTAGATACATGTAATTAAAAACAAATAAACAAACAAAAAAAAACAAA19920.11922696396795955No Hit
ATAAAACACAGTATCATGCAAAAACATGAATGAAACTAAAAACAATGTCA19770.11832917056458635No Hit
GCTCTGGGACACTCATTCTCTGGACATGAGTCCTGAGGCTGCACGCAAGT19650.11761093584188782No Hit
GCATTATTTTAGAGTAACAAGATATTAGACCCAGGTGACAGTCCTGAGAT19320.11563579035446679No Hit
CTTTAAAGGCTTGAATAATGCTATAAAACTCTTACAGTAGAGTCATTAAC19200.11491755563176824No Hit
GAACTACAGGAGCGCCTTGGTGGCTCAGTCATTAAATGTCTGTCTTTGGC19110.11437887958974433No Hit
CTACAGGAGCGCCTTGGTGGCTCAGTCATTAAATGTCTGTCTTTGGCTCA19110.11437887958974433No Hit
ATTTTTATCTCTGCTGCTGTTTCAAACGGATAGTTATCTTTGCTTCCCAG19040.11395990933483684No Hit
ACATACAGACATACATTTAAAACTTGTTTGGCTGGTTATTCCTTGTTCAT18920.11324167461213829No Hit
CTGTATGTCTTCCAAAAGGTACTGTGACCTTCTGAAGATTTAGATCATCA18860.112882557250789No Hit
GCATAGAGGAGGAGACGTCGAGTTGGTGGTGAGTAGCAGATGTGAACGAT18830.11270299857011436No Hit
GTTAAAGCATCAGTCACAAGGGAAAATGAGCTTTTTCCGCAGCGTGAAGG18660.11168549937962476No Hit
GAGTTTATATTTGGTTTGCTTGCTTGCAGCTTTTCATTTTCAAATTTAGG18340.10977020678576196No Hit
GTGTTTGCATGTTGCGACGGGTGCCTGGCTTAGCATAGAGGAGGAGACGT18230.10911182495662161No Hit
GGGTTAAGCCGCTGCCTTCGGCTCAGGTCATGATCCCAGGGTCCCGGGAT18210.1089921191695052No Hit
ATGCAAACACAGTACCTTCTAATTTGTAACCTGCTTCCTCTGCATTTAGT18200.10893226627594697No Hit
GTCTTCCACACGGTTTTATTGCATAGAAACACAACAATTTAGATCTCAGG18180.10881256048883056No Hit
GTCTAATATCTTGTTACTCTAAAATAATGCAGCAGGGCCCCTGGGTGGCT18040.10797461997901557No Hit
ATACAAGTAATGCTGCTATGAAATTCTGGGGTTTTTTTTCTTTTTTAATT17930.10731623814987525No Hit
TGCAAACACAGTACCTTCTAATTTGTAACCTGCTTCCTCTGCATTTAGTG17890.1070768265756424No Hit
ATCTAATACCTTCAGTGCTTTAAATATATAATTGCCTCCATAAGAAGATC17810.10659800342717668No Hit
TTTCAAATACTGTATAATTTAGTATTTCAGACCCAATCTTCTCCCCAACC17740.1061790331722692No Hit
GATCTAAATTGTTGTGTTTCTATGCAATAAAACCGTGTGGAAGACTGTGT17690.10587976870447813No Hit
CCTTAATACCCACCACCAGTGTCACCCATCCTCCCACCCGTCTCCTCTCC17620.10546079844957065No Hit
ATGCAGATGTCTGTCCACACAATTGATAAGCTTCATAACAGCTGAAAATG17480.10462285793975566No Hit
ATCATGACCTGAGCCAAAGGCAGACACTTAACAACTGAGCCACCCAGGGG17460.10450315215263925No Hit
TCTTGGAACACTGAAAAAATTAAAAAAGAAAAAAAACCCCAGAATTTCAT17390.10408418189773176No Hit
GTCTTTATGTTAGTGCCTCATTAGAGGGAAAGTGGCCTTGGCTTGATAGT17340.1037849174299407No Hit
CTGTAAGTACTATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGT17290.10348565296214962No Hit
CTATAAAACTCTTACAGTAGAGTCATTAACTAAAAAGAACAGAGGGTTGC17160.10270756534589286No Hit
CAAGTAGGCAGAGAGGCAGACAGAGAGAGGAGGAAGCAGGCTCCCTGCCG17120.10246815377166002No Hit
ACTTGATACAGTAATTTTAAAAAAAAGGAAGACCTATGCCTGCAAGGCCA17090.10228859509098537No Hit
GTGAGAAATAATGTTAACTTTGTCACAGTAAATGCGATTCCAACCAGACC17040.1019893306231943No Hit
GTGTATGCCACTGAACACAGTCTTCCACACGGTTTTATTGCATAGAAACA16730.10013389092288973No Hit
TCTCTAACCTACTTGATACCTGGGAAGCAAAGATAACTATCCGTTTGAAA16720.1000740380293315No Hit
GAGTTTTATAGCATTATTCAAGCCTTTAAAGTTAGCAGTTAAATGATGAT16720.1000740380293315No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)