FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_D9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_D9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5442519
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAAGACTGAATGGAAACTGAACGGACAGGTGCTGGTCTTCACACTCC239930.4408436608122085No Hit
AAGCAGGACAGTGGGAGACAGGAAACGGCAAAAACGGCAGTGGCTGAGAC236780.43505589966704755No Hit
CTCATATACTGCACCCATTTCAGCAGCAAATCCTGTTGCCCCTACTTTCA155720.28611751286490683No Hit
GTGGGAGACAGGAAACGGCAAAAACGGCAGTGGCTGAGACCTGACAGCAA153930.28282859462686305No Hit
GTATATGAGAAGAAAAAAGAAATGCAGAATAACACAAAGATTCTAGACAA137520.2526771151373105No Hit
GTAATAAGCCAATGAGTTGGAATCCTTGATGAAAATGCCCTCATACTGCA130330.2394663206504194No Hit
CCCTCATACTGCAGCTTCTGTTTCCCTGCAGGCATACCCGTGGCTTCGTG108940.20016466639804106No Hit
AAACAAGGGTCCAGTGTCCATCAAAGTCCAAGTGCCCAACATGCAGGATA102250.1878725641564136No Hit
GTCCAGTGTCCATCAAAGTCCAAGTGCCCAACATGCAGGATAAGACTGAA98490.18096399847203104No Hit
GACCAGGTCTCTGTCATCAAGGTGAAGATTCACGAAGCCACGGGTATGCC97980.18002693238186213No Hit
GTCCATCAAAGTCCAAGTGCCCAACATGCAGGATAAGACTGAATGGAAAC97790.17967782932866197No Hit
GTGCTGGTCTTCACACTCCCGCTCACAGACCAGGTCTCTGTCATCAAGGT96260.17686663105815523No Hit
CTCCTTGAGGGCCAGGTGGATGATGGCGCCGTTGGCCATGTTGTAATAAG92550.17004993459829906No Hit
GTTGTAATAAGCCAATGAGTTGGAATCCTTGATGAAAATGCCCTCATACT87390.16056903062717834No Hit
GTATGAGGGCATTTTCATCAAGGATTCCAACTCATTGGCTTATTACAACA85170.1564900370582078No Hit
ACCTAGGAGACACCAGTGGCTTGGACCAGCATAGCTGAAGCACACACGGT84600.15544272789860725No Hit
GGCTGAGACCTGACAGCAAGTTTCTCTTCTCTACTTCTTCCGCCCGCCTC82970.1524477911790478No Hit
GGCATACCCGTGGCTTCGTGAATCTTCACCTTGATGACAGAGACCTGGTC73720.13545198464167052No Hit
GTAGAAACAAGGGTCCAGTGTCCATCAAAGTCCAAGTGCCCAACATGCAG72080.1324386740772058No Hit
ATTCTAGACAATGAGCTTTTTCTTTCCAGACACAGGTGCTGCTCCTTCTT67760.12450117307812797No Hit
CCATAAAATTCGCCCTTTTAGAACTGTACAACTCAGTGATTTTAGTCTTT66240.12170834865252653No Hit
TGTTGTAATAAGCCAATGAGTTGGAATCCTTGATGAAAATGCCCTCATAC65590.12051404873368379No Hit
GTCTAGAATCTTTGTGTTATTCTGCATTTCTTTTTTCTTCTCATATACTG65480.12031193643972579No Hit
CTCTTAATCTTGGCTTAAATAAATAGGGTGCTTATTTTTCTTCTGTAAGA64020.11762935508355599No Hit
TCACACTCCCGCTCACAGACCAGGTCTCTGTCATCAAGGTGAAGATTCAC61250.11253980004479543No Hit
GTATGTTATTGTCCTCATGCTTGTCTCTGTAGGTTACCAAATACCTGTTC59810.10989396637843617No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA59680.10965510639466762No Hit
GACTATAGGCACGCCAGAGTGGGAGCAGGGAGAGGAAACATTGGCCATTA59500.10932437718637271No Hit
GACAGGTGCTGGTCTTCACACTCCCGCTCACAGACCAGGTCTCTGTCATC59200.10877316183921452No Hit
ATAATAACCTAGGAGACACCAGTGGCTTGGACCAGCATAGCTGAAGCACA59150.10868129261468816No Hit
CCTCTCTCCTTGAGGGCCAGGTGGATGATGGCGCCGTTGGCCATGTTGTA59060.1085159280105407No Hit
GGTCTCTGTCATCAAGGTGAAGATTCACGAAGCCACGGGTATGCCTGCAG58960.10833218956148798No Hit
GGGTATATGGAATCTATATCAAACAAGAAAGGCAGGAGGAAGATAGAAGC57830.10625594508719216No Hit
ATAACATACTAAGGATTTCTGAATGGAAAGGCAGAAATAGCCTCCTGGCT57680.10598033741361307No Hit
GTGGATGATGGCGCCGTTGGCCATGTTGTAATAAGCCAATGAGTTGGAAT57410.1054842436011707No Hit
GGGTATGCCTGCAGGGAAACAGAAGCTGCAGTATGAGGGCATTTTCATCA57090.10489628056420197No Hit
GAGTTGGAATCCTTGATGAAAATGCCCTCATACTGCAGCTTCTGTTTCCC55760.10245255919180071No Hit
TCTCTACTTCTTCCGCCCGCCTCTCTCCTTGAGGGCCAGGTGGATGATGG55680.10230556843255853No Hit
GAATGGAAACTGAACGGACAGGTGCTGGTCTTCACACTCCCGCTCACAGA54810.1007070439257998No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)