FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_D8a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_D8a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences879024
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGAGTTTATGTAGTTCCAGAAGAGAATTGCTGGATCATATTGCAT151951.7286217441162015No Hit
ATGTATAGGAGTTTATGTAGTTCCAGAAGAGAATTGCTGGATCATATTGC126471.4387548007790458No Hit
GTATAAAGTATTAGGATACGGATCTGCAGATTCTCCAGTACACTGTTGTT113871.2954140046233096No Hit
GTATTAGGATACGGATCTGCAGATTCTCCAGTACACTGTTGTTGGTACTA109001.2400116492837512No Hit
AGTTTATGTAGTTCCAGAAGAGAATTGCTGGATCATATTGCATACCACAT94221.0718706201423396No Hit
GTTGTTGGTACTATAAATTGGTGAACTTGTTACCACTTTGGAAAACTCCT81540.9276197236935511No Hit
CTCCAGTACACTGTTGTTGGTACTATAAATTGGTGAACTTGTTACCACTT78800.8964487886565099No Hit
ATATTGCATACCACATACTCAACTTTAGGAGTTTTCCAAAGTGGTAACAA78490.8929221500209323No Hit
ATCATATTGCATACCACATACTCAACTTTAGGAGTTTTCCAAAGTGGTAA70130.7978166693969675No Hit
GTTCTAGGACTCGGAGTTCATAACTCAAACCAAAGGCAGACACTTAGCTG68310.7771118877300279No Hit
TCCTAATACTTTATACGACGAGACTTTAAAATCAAGCTCCTGAGTGGCTC58680.6675585649538579No Hit
CACACACACAGAGGGCACAAGCAGGCAGAGGGAGATCGAGAAGAAGCAGG54470.6196645370319809No Hit
CACACACAGAGGGCACAAGCAGGCAGAGGGAGATCGAGAAGAAGCAGGCT53000.602941444147145No Hit
ATTTTAAAGTCTCGTCGTATAAAGTATTAGGATACGGATCTGCAGATTCT44620.5076084384499172No Hit
ACTTTATACGACGAGACTTTAAAATCAAGCTCCTGAGTGGCTCAGTCAGC43790.49816614790949965No Hit
CTGTTGTTGGTACTATAAATTGGTGAACTTGTTACCACTTTGGAAAACTC43720.49736981015307885No Hit
GTATCAGACTTCCCCTGCTCTGCGGGGAGCCTGCTTCTTCTCGATCTCCC42710.48587979395329367No Hit
ACTCAGGAGCTTGATTTTAAAGTCTCGTCGTATAAAGTATTAGGATACGG41390.4708631391179308No Hit
CCACATACTCAACTTTAGGAGTTTTCCAAAGTGGTAACAAGTTCACCAAT39960.45459509637962103No Hit
GTACTATAAATTGGTGAACTTGTTACCACTTTGGAAAACTCCTAAAGTTG39830.45311618340341103No Hit
CACACAGAGGGCACAAGCAGGCAGAGGGAGATCGAGAAGAAGCAGGCTCC38900.4425362674966782No Hit
GAGTTTATGTAGTTCCAGAAGAGAATTGCTGGATCATATTGCATACCACA38660.43980596661752125No Hit
GCATACCACATACTCAACTTTAGGAGTTTTCCAAAGTGGTAACAAGTTCA37890.4310462512968929No Hit
ACACTGTTGTTGGTACTATAAATTGGTGAACTTGTTACCACTTTGGAAAA35720.40635978084784946No Hit
TGTTACCACTTTGGAAAACTCCTAAAGTTGAGTATGTGGTATGCAATATG35150.39987531625985184No Hit
ATAAATTGGTGAACTTGTTACCACTTTGGAAAACTCCTAAAGTTGAGTAT34750.3953248147945903No Hit
GTACACTGTTGTTGGTACTATAAATTGGTGAACTTGTTACCACTTTGGAA34270.3898642130362766No Hit
CCAGTACACTGTTGTTGGTACTATAAATTGGTGAACTTGTTACCACTTTG33520.3813320227889113No Hit
CTATAAATTGGTGAACTTGTTACCACTTTGGAAAACTCCTAAAGTTGAGT31130.3541427765339741No Hit
GAACAGGAGAGAGGAACAGAGGGAAAGGGAGAAGGAGACTCCACAGTGAG30970.3523225759478694No Hit
CCTTTTACACGCACTCCGCGCTACGAACAAATATACCGGCGGGCTGCTCC30060.34197018511439964No Hit
ACCAACAACAGTGTACTGGAGAATCTGCAGATCCGTATCCTAATACTTTA29450.3350306703798759No Hit
GTACTGGAGAATCTGCAGATCCGTATCCTAATACTTTATACGACGAGACT29290.3332104697937713No Hit
TCCGTATCCTAATACTTTATACGACGAGACTTTAAAATCAAGCTCCTGAG27700.3151222264693569No Hit
CTCCTAAAGTTGAGTATGTGGTATGCAATATGATCCAGCAATTCTCTTCT27450.31227816305356854No Hit
GGGCAAGAGAGAAGCAGACTCCCTGCTGAGCAGGAAGCCCAATGCGGGGC25540.290549518556945No Hit
CTTAGCTGACTGAGCCACTCAGGAGCTTGATTTTAAAGTCTCGTCGTATA25400.28895684304410346No Hit
ACTTTAGGAGTTTTCCAAAGTGGTAACAAGTTCACCAATTTATAGTACCA25030.2847476291887366No Hit
GGATACGGATCTGCAGATTCTCCAGTACACTGTTGTTGGTACTATAAATT24880.28304119113926357No Hit
CTTTAGGAGTTTTCCAAAGTGGTAACAAGTTCACCAATTTATAGTACCAA24730.2813347530897905No Hit
ATGTAGTTCCAGAAGAGAATTGCTGGATCATATTGCATACCACATACTCA24610.27996960265021203No Hit
GTCCAACACGGTACCTGAACCCAGGACTCCAGGATCAAGACCTGAGCTGA24570.2795145525036859No Hit
AGCGAAAGCAGGGCAAGAGAGAAGCAGACTCCCTGCTGAGCAGGAAGCCC24030.27337137552558294No Hit
GGATCATATTGCATACCACATACTCAACTTTAGGAGTTTTCCAAAGTGGT22330.2540317442982217No Hit
ATTGCATACCACATACTCAACTTTAGGAGTTTTCCAAAGTGGTAACAAGT22230.2528941189319063No Hit
TGTTGGTACTATAAATTGGTGAACTTGTTACCACTTTGGAAAACTCCTAA22130.25175649356559093No Hit
ATGCAATATGATCCAGCAATTCTCTTCTGGAACTACATAAACTCCTATAC21650.24629589180727718No Hit
GTCTGATACAGGACTCGATTTCATGACCAGAGCTGAAGGCAGTGGCTCAG21470.24424816614790948No Hit
ATACTCAACTTTAGGAGTTTTCCAAAGTGGTAACAAGTTCACCAATTTAT21010.2390150894628588No Hit
ATGCTTAACTGACTGAGCCACTGCCTTCAGCTCTGGTCATGAAATCGAGT20970.23856003931633263No Hit
TTCCAGAAGAGAATTGCTGGATCATATTGCATACCACATACTCAACTTTA20910.23787746409654345No Hit
AAGTATTAGGATACGGATCTGCAGATTCTCCAGTACACTGTTGTTGGTAC20650.2349196381441235No Hit
GAGAGAAGCAGACTCCCTGCTGAGCAGGAAGCCCAATGCGGGGCTCTGTT20100.22866269862938893No Hit
ACATAAACTCCTATACATTTTCTTTTCTTTCTTTCCTTTTTTTAAAGATT20060.22820764848286282No Hit
GATCTGCAGATTCTCCAGTACACTGTTGTTGGTACTATAAATTGGTGAAC19990.22741131072644202No Hit
GGTCAATGATTTGTTACGAGTGAAGGGCATCGGAGTACGAGGCTTGAAGA19870.2260461602868636No Hit
AGCGAAAGCAGGTCAATGATTTGTTACGAGTGAAGGGCATCGGAGTACGA19640.22342962194433824No Hit
ACTATAAATTGGTGAACTTGTTACCACTTTGGAAAACTCCTAAAGTTGAG19570.2226332841879175No Hit
GTACCTGAACCCAGGACTCCAGGATCAAGACCTGAGCTGAAGGCAGATGC19550.22240575911465443No Hit
GCCCAAGGCTTCGGCCTGATTGTTCGATTCGAAATGCTGAACGCCGGGAC19430.221040608675076No Hit
GAGTACGAGGCTTGAAGAAGCTCATGCCGCACGTCGTGCTCGACCCGCCC18670.2123946558910792No Hit
GGAGAGAGGAACAGAGGGAAAGGGAGAAGGAGACTCCACAGTGAGCAGGG18360.20886801725550158No Hit
GTGGTAACAAGTTCACCAATTTATAGTACCAACAACAGTGTACTGGAGAA17790.20238355266750394No Hit
GAAAATGTATAGGAGTTTATGTAGTTCCAGAAGAGAATTGCTGGATCATA17540.19953948925171555No Hit
GTGGTATGCAATATGATCCAGCAATTCTCTTCTGGAACTACATAAACTCC17440.19840186388540015No Hit
AGTATGTGGTATGCAATATGATCCAGCAATTCTCTTCTGGAACTACATAA17430.19828810134876865No Hit
GTTTATGTAGTTCCAGAAGAGAATTGCTGGATCATATTGCATACCACATA17370.19760552612897941No Hit
GCCCAATGCGGGGCTCTGTTCTAGGACTCGGAGTTCATAACTCAAACCAA17190.19555780046961174No Hit
CTGTGTGTGTGTGTCAAATAAATAAATATAATCTTTAAAAAAAGGAAAGA16740.19043848632119262No Hit
GTATATTTGTTCGTAGCGCGGAGTGCGTGTAAAAGGAGCGCTTCAGCGTG16720.19021096124792952No Hit
TTTCCAAAGTGGTAACAAGTTCACCAATTTATAGTACCAACAACAGTGTA16570.18850452319845648No Hit
GCTCTGCGGGGAGCCTGCTTCTTCTCGATCTCCCTCTGCCTGCTTGTGCC16400.18657056007572034No Hit
TTATAGTACCAACAACAGTGTACTGGAGAATCTGCAGATCCGTATCCTAA16360.1861155099291942No Hit
GTGTGTGTGTGTCAAATAAATAAATATAATCTTTAAAAAAAGGAAAGAAA16340.18588798485593114No Hit
GCCTTCAGCTCTGGTCATGAAATCGAGTCCTGTATCAGACTTCCCCTGCT16030.18236134622035347No Hit
GTTAAGCATCTGCCTTCAGCTCAGGTCTTGATCCTGGAGTCCTGGGTTCA15960.18156500846393273No Hit
AAAGCAGGGAGGCTCGCAGCTTGCTGGCGGGTGGATCGAGCGGTCGGCCC15080.1715539052403575No Hit
TCATAACTCAAACCAAAGGCAGACACTTAGCTGACTGAGCCACTCAGGAG15050.17121261763046286No Hit
CTCCTGAGTGGCTCAGTCAGCTAAGTGTCTGCCTTTGGTTTGAGTTATGA14930.16984746719088442No Hit
GGCAGAGGGAGATCGAGAAGAAGCAGGCTCCCCGCAGAGCAGGGGAAGTC14880.16927865450772675No Hit
GGGCACAAGCAGGCAGAGGGAGATCGAGAAGAAGCAGGCTCCCCGCAGAG14000.15926755128415152No Hit
ACTCCACAGTGAGCAGGGAGTCCAACACGGTACCTGAACCCAGGACTCCA13940.1585849760643623No Hit
CTCCTATACATTTTCTTTTCTTTCTTTCCTTTTTTTAAAGATTATATTTA13630.15505833742878464No Hit
GCTCAGTGAGGTTGCAAGCACGCTGCAGCTGACGATGGCGAAGGTTCTGG13580.15448952474562697No Hit
GATTTGTTACGAGTGAAGGGCATCGGAGTACGAGGCTTGAAGAAGCTCAT13550.15414823713573236No Hit
CACTTAGCTGACTGAGCCACTCAGGAGCTTGATTTTAAAGTCTCGTCGTA13480.1533518993793116No Hit
CTGTTCTAGGACTCGGAGTTCATAACTCAAACCAAAGGCAGACACTTAGC13350.15187298640310162No Hit
CAAATATACCGGCGGGCTGCTCCGAGCGCGGCCCGCCGGTCCCGGCGTTC13300.15130417371994395No Hit
GAGTATGTGGTATGCAATATGATCCAGCAATTCTCTTCTGGAACTACATA13260.1508491235734178No Hit
CTTCAGCTCTGGTCATGAAATCGAGTCCTGTATCAGACTTCCCCTGCTCT13090.14891516045068168No Hit
CTTTGGAAAACTCCTAAAGTTGAGTATGTGGTATGCAATATGATCCAGCA13070.1486876353774186No Hit
CTCTGTGTGTGTGTGTCAAATAAATAAATATAATCTTTAAAAAAAGGAAA12990.14777753508436628No Hit
CCCCTGCTCTGCGGGGAGCCTGCTTCTTCTCGATCTCCCTCTGCCTGCTT12970.14755001001110324No Hit
TTGCATACCACATACTCAACTTTAGGAGTTTTCCAAAGTGGTAACAAGTT12590.14322703361910483No Hit
CACAGAGGGCACAAGCAGGCAGAGGGAGATCGAGAAGAAGCAGGCTCCCC12510.14231693332605252No Hit
GTTCCAGAAGAGAATTGCTGGATCATATTGCATACCACATACTCAACTTT12480.14197564571615792No Hit
AACCAAAGGCAGACACTTAGCTGACTGAGCCACTCAGGAGCTTGATTTTA12460.14174812064289485No Hit
CAACAACAGTGTACTGGAGAATCTGCAGATCCGTATCCTAATACTTTATA12440.14152059556963179No Hit
GTGAGCAGGGAGTCCAACACGGTACCTGAACCCAGGACTCCAGGATCAAG12440.14152059556963179No Hit
AATTTATAGTACCAACAACAGTGTACTGGAGAATCTGCAGATCCGTATCC12380.14083802034984255No Hit
GTGTACTGGAGAATCTGCAGATCCGTATCCTAATACTTTATACGACGAGA12260.1394728699102641No Hit
CTTGTTACCACTTTGGAAAACTCCTAAAGTTGAGTATGTGGTATGCAATA12110.13776643186079107No Hit
GAGCTGAAGGCAGTGGCTCAGTCAGTTAAGCATCTGCCTTCAGCTCAGGT12110.13776643186079107No Hit
AGCAGGAACAGGAGAGAGGAACAGAGGGAAAGGGAGAAGGAGACTCCACA12050.13708385664100184No Hit
GTTCTGGCTCGAGGCAGCACGCTGAAGCGCTCCTTTTACACGCACTCCGC12040.1369700941043703No Hit
TTGCTGGATCATATTGCATACCACATACTCAACTTTAGGAGTTTTCCAAA11980.1362875188845811No Hit
GAAAGAAAAGAAAATGTATAGGAGTTTATGTAGTTCCAGAAGAGAATTGC11890.13526365605489724No Hit
CTCCCTGCTGAGCAGGAAGCCCAATGCGGGGCTCTGTTCTAGGACTCGGA11770.1338985056153188No Hit
GAACAGAGGGAAAGGGAGAAGGAGACTCCACAGTGAGCAGGGAGTCCAAC11700.13310216785889806No Hit
TGTAAAAGGAGCGCTTCAGCGTGCTGCCTCGAGCCAGAACCTTCGCCATC11630.13230583010247732No Hit
ACACTTAGCTGACTGAGCCACTCAGGAGCTTGATTTTAAAGTCTCGTCGT11590.13185077995595115No Hit
GTTCATAACTCAAACCAAAGGCAGACACTTAGCTGACTGAGCCACTCAGG11530.13116820473616192No Hit
GAAGAGAATTGCTGGATCATATTGCATACCACATACTCAACTTTAGGAGT11440.13014434190647808No Hit
AGTGAAGGGCATCGGAGTACGAGGCTTGAAGAAGCTCATGCCGCACGTCG11320.12877919146689965No Hit
ATATTTATTTATTTGACACACACACACAGAGGGCACAAGCAGGCAGAGGG11210.12752780356395274No Hit
GGCAGACACTTAGCTGACTGAGCCACTCAGGAGCTTGATTTTAAAGTCTC11070.12593512805111123No Hit
TATTGCATACCACATACTCAACTTTAGGAGTTTTCCAAAGTGGTAACAAG11020.12536631536795356No Hit
GAAAAGAAAATGTATAGGAGTTTATGTAGTTCCAGAAGAGAATTGCTGGA11010.12525255283132203No Hit
GTATGCAATATGATCCAGCAATTCTCTTCTGGAACTACATAAACTCCTAT10810.12297730209869127No Hit
GAGAGGAACAGAGGGAAAGGGAGAAGGAGACTCCACAGTGAGCAGGGAGT10730.12206720180563899No Hit
ACCACATACTCAACTTTAGGAGTTTTCCAAAGTGGTAACAAGTTCACCAA10620.12081581390269208No Hit
ACAGTGTACTGGAGAATCTGCAGATCCGTATCCTAATACTTTATACGACG10620.12081581390269208No Hit
GACTGAGCCACTGCCTTCAGCTCTGGTCATGAAATCGAGTCCTGTATCAG10610.12070205136606055No Hit
CCTATACATTTTCTTTTCTTTCTTTCCTTTTTTTAAAGATTATATTTATT10550.12001947614627131No Hit
TCCTAGAACAGAGCCCCGCATTGGGCTTCCTGCTCAGCAGGGAGTCTGCT10530.11979195107300826No Hit
TACACGCACTCCGCGCTACGAACAAATATACCGGCGGGCTGCTCCGAGCG10490.1193369009264821No Hit
AGCGAAAGCAGGGAGGCTCGCAGCTTGCTGGCGGGTGGATCGAGCGGTCG10440.11876808824332441No Hit
GCTCTGTTCTAGGACTCGGAGTTCATAACTCAAACCAAAGGCAGACACTT10370.11797175048690366No Hit
CTCAGGAGCTTGATTTTAAAGTCTCGTCGTATAAAGTATTAGGATACGGA9990.11364877409490526No Hit
GAGAAGCAGACTCCCTGCTGAGCAGGAAGCCCAATGCGGGGCTCTGTTCT9850.11205609858206374No Hit
CTCCAGGATCAAGACCTGAGCTGAAGGCAGATGCTTAACTGACTGAGCCA9840.1119423360454322No Hit
GACTGAGCCACTCAGGAGCTTGATTTTAAAGTCTCGTCGTATAAAGTATT9790.11137352336227453No Hit
CTTTAAAATCAAGCTCCTGAGTGGCTCAGTCAGCTAAGTGTCTGCCTTTG9780.111259760825643No Hit
GGTAACAAGTTCACCAATTTATAGTACCAACAACAGTGTACTGGAGAATC9770.11114599828901145No Hit
ATGCGGGGCTCTGTTCTAGGACTCGGAGTTCATAACTCAAACCAAAGGCA9760.11103223575237992No Hit
GAAGCAGACTCCCTGCTGAGCAGGAAGCCCAATGCGGGGCTCTGTTCTAG9690.11023589799595915No Hit
TACTCAACTTTAGGAGTTTTCCAAAGTGGTAACAAGTTCACCAATTTATA9640.10966708531280148No Hit
CTGCTGAGCAGGAAGCCCAATGCGGGGCTCTGTTCTAGGACTCGGAGTTC9520.10830193487322304No Hit
GATTATATTTATTTATTTGACACACACACACAGAGGGCACAAGCAGGCAG9490.10796064726332842No Hit
AAATTGGTGAACTTGTTACCACTTTGGAAAACTCCTAAAGTTGAGTATGT9480.10784688472669687No Hit
GAATTGCTGGATCATATTGCATACCACATACTCAACTTTAGGAGTTTTCC9480.10784688472669687No Hit
ACTCAAACCAAAGGCAGACACTTAGCTGACTGAGCCACTCAGGAGCTTGA9470.10773312219006534No Hit
TTGGTGAACTTGTTACCACTTTGGAAAACTCCTAAAGTTGAGTATGTGGT9350.1063679717504869No Hit
CCAATTTATAGTACCAACAACAGTGTACTGGAGAATCTGCAGATCCGTAT9320.10602668414059228No Hit
GGGAGAAGGAGACTCCACAGTGAGCAGGGAGTCCAACACGGTACCTGAAC9200.10466153370101385No Hit
GCCTTGGGCGGGTCGAGCACGACGTGCGGCATGAGCTTCTTCAAGCCTCG9140.10397895848122463No Hit
GGGAAAGGGAGAAGGAGACTCCACAGTGAGCAGGGAGTCCAACACGGTAC9040.10284133311490927No Hit
ATCCTGGAGTCCTGGGTTCAGGTACCGTGTTGGACTCCCTGCTCACTGTG9010.10250004550501465No Hit
GTTGAGTATGTGGTATGCAATATGATCCAGCAATTCTCTTCTGGAACTAC9000.10238628296838312No Hit
GAGCAGGGGAAGTCTGATACAGGACTCGATTTCATGACCAGAGCTGAAGG8970.1020449953584885No Hit
ATTTATAGTACCAACAACAGTGTACTGGAGAATCTGCAGATCCGTATCCT8970.1020449953584885No Hit
GCTGAAGGCAGATGCTTAACTGACTGAGCCACTGCCTTCAGCTCTGGTCA8960.10193123282185695No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)