FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_D8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_D8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4540702
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAGCAAAGCTCACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTTT318740.7019619433294676No Hit
ATCCTGGAGTCCTGGGACCGAGTCCTACGTCAGGCTCCCTGTTCGGCGGG273000.6012286205965509No Hit
GAGAGAGAGAGTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCG262710.578566926435604No Hit
GTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGG249610.5497167618575277No Hit
CACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTTTTATGTCAGTCAAG242850.5348291960141847No Hit
GCTCACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTTTTATGTCAGTC242130.5332435381137102No Hit
ATTTGAGAGAGAGAGTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTC231420.5096568768441532No Hit
GTCCCAGGACTCCAGGATCATGACCTGAGCCAAAGGCAGTTGCTCAACCA229670.5058028472249445No Hit
GGTCATGATCCTGGAGTCCTGGGACCGAGTCCTACGTCAGGCTCCCTGTT217160.47825204120420145No Hit
GGTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGGAGCCTGACGTAGGAC212840.46873809380135495No Hit
AGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGGAGCCT212100.4671083898480896No Hit
CTTTTATGTCAGTCAAGAAAGCAGAAATGAGGGGCACCTGGGTGGCTCAG207230.45638317599349176No Hit
CTCCAGGATCATGACCTGAGCCAAAGGCAGTTGCTCAACCAACTGAGCCA205460.45248510032149214No Hit
GTCCTGGGACCGAGTCCTACGTCAGGCTCCCTGTTCGGCGGGGAGTCTGC205240.45200059374079166No Hit
GAAATGAGGGGCGCCTGGGTGGCTCAGTTGGTTAAGCCGCTGCAGCTCGG197970.43598985355127906No Hit
ATCATGACCTGAGCCAAAGGCAGTTGCTCAACCAACTGAGCCACCCAGGT194400.4281276331280934No Hit
ACGTAGGACTCGGTCCCAGGACTCCAGGATCATGACCTGAGCCAAAGGCA180250.396965050778492No Hit
GTCCTACGTCAGGCTCCCTGTTCGGCGGGGAGTCTGCGCTTCCTCTGACC176790.38934508364565656No Hit
GGCATAGTGGCAGGCAGAAGCAGAGGGAGACATAGGCTCCCTGCTGAGCA159930.3522142611428805No Hit
GCTTTTATGTCAGTCAAGAAAGCAGAAATGAGGGGCACCTGGGTGGCTCA155620.3427223367664295No Hit
CCCTGAGATCATGACCCGAGCTGCAGCGGCTTAACCAACTGAGCCACCCA152380.3355868762142946No Hit
GACATAGGCTCCCTGCTGAGCAAGGATCCTCTGGTGGGACTCCATCTCCA148780.3276585867119225No Hit
GAAATGAGGGGCACCTGGGTGGCTCAGTTGGTTGAGCAACTGCCTTTGGC143940.31699944193651114No Hit
GAGTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAACAG140200.30876283006460237No Hit
CACCTGGGTGGCTCAGTTGGTTGAGCAACTGCCTTTGGCTCAGGTCATGA139890.3080801162463425No Hit
CGCCTGGGTGGCTCAGTTGGTTAAGCCGCTGCAGCTCGGGTCATGATCTC138030.3039838333367836No Hit
GAGAGAGAGTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCC137450.30270649780584585No Hit
GGGATAGCAAAGCTCACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTT134290.2957472214648748No Hit
GTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGGAGCCTGACGTAGGACT132550.2919152148720616No Hit
GCCTTTGGCTCAGGTCATGATCCTGGAGTCCTGGGACCGAGTCCTACGTC123260.27145582335066254No Hit
ATTTATTTGAGAGAGAGAGTGTGAGAATGGAGAGGTCAGAGGAAGCGCAG122090.2688791292623916No Hit
AAAGCAGGGATAGCAAAGCTCACAAGAACGTGTCAGGGAAAACTGGCTGT121500.26757977070505834No Hit
TCTCCAGACCCTGAGATCATGACCCGAGCTGCAGCGGCTTAACCAACTGA119460.26308707332038084No Hit
TTCTCACACTCTCTCTCTCAAATAAATAAATAAAATTTAAAAAAAAAAAA117400.2585503298829124No Hit
ATCATGACCCGAGCTGCAGCGGCTTAACCAACTGAGCCACCCAGGCGCCC115920.25529092197638165No Hit
CTCCAGACCCTGAGATCATGACCCGAGCTGCAGCGGCTTAACCAACTGAG115630.2546522542109128No Hit
ATCTCAGGGTCTGGAGATGGAGTCCCACCAGAGGATCCTTGCTCAGCAGG108900.23983075744675605No Hit
GCATAGTGGCAGGCAGAAGCAGAGGGAGACATAGGCTCCCTGCTGAGCAA106710.23500771466614634No Hit
CTCCATTCTCACACTCTCTCTCTCAAATAAATAAATAAAATTTAAAAAAA104730.2306471554398417No Hit
AGCAGGGATAGCAAAGCTCACAAGAACGTGTCAGGGAAAACTGGCTGTGA104490.23011860280635021No Hit
GGTTAAGCCGCTGCAGCTCGGGTCATGATCTCAGGGTCTGGAGATGGAGT102000.22463486923387618No Hit
TAGCAAAGCTCACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTTTTAT100600.22155164553850923No Hit
AGCGAAAGCAGGGATAGCAAAGCTCACAAGAACGTGTCAGGGAAAACTGG95470.21025383299762898No Hit
GTGGCAGGCAGAAGCAGAGGGAGACATAGGCTCCCTGCTGAGCAAGGATC92620.20397727047491773No Hit
GGTCATGATCTCAGGGTCTGGAGATGGAGTCCCACCAGAGGATCCTTGCT89420.1969299020283648No Hit
GCAGAAATGAGGGGCACCTGGGTGGCTCAGTTGGTTGAGCAACTGCCTTT89380.19684180992278286No Hit
GGGTGGCTCAGTTGGTTGAGCAACTGCCTTTGGCTCAGGTCATGATCCTG88060.19393477043857976No Hit
AGCAGGCATAGTGGCAGGCAGAAGCAGAGGGAGACATAGGCTCCCTGCTG86050.18950814213308867No Hit
GCCAAAGGCAGTTGCTCAACCAACTGAGCCACCCAGGTGCCCCTCATTTC84450.1859844579098122No Hit
GGGAAAACTGGCTGTGAGCTTTTATGTCAGTCAAGAAAGCAGAAATGAGG83280.18340776382154125No Hit
GCACCTGGGTGGCTCAGTTGGTTGAGCAACTGCCTTTGGCTCAGGTCATG83180.1831875335575865No Hit
GGCAGAAGCAGAGGGAGACATAGGCTCCCTGCTGAGCAAGGATCCTCTGG81330.17911327367442303No Hit
AAAGCAGGCATAGTGGCAGGCAGAAGCAGAGGGAGACATAGGCTCCCTGC78420.1727045729933389No Hit
GTGTCAGGGAAAACTGGCTGTGAGCTTTTATGTCAGTCAAGAAAGCAGAA77990.17175758285833334No Hit
ACCCAGGTGCCCCTCATTTCTGCTTTCTTGACTGACATAAAAGCTCACAG77920.17160342167356502No Hit
GCGCCTGGGTGGCTCAGTTGGTTAAGCCGCTGCAGCTCGGGTCATGATCT77830.1714052144360057No Hit
TGTCAGGGAAAACTGGCTGTGAGCTTTTATGTCAGTCAAGAAAGCAGAAA76000.1673750006056332No Hit
AGCGCAGACTCCCCGCCGAACAGGGAGCCTGACGTAGGACTCGGTCCCAG74510.164093569672707No Hit
CATAGGCTCCCTGCTGAGCAAGGATCCTCTGGTGGGACTCCATCTCCAGA74280.163587040065611No Hit
GAGGAAGCGCAGACTCCCCGCCGAACAGGGAGCCTGACGTAGGACTCGGT73260.16134069137327225No Hit
GAGGGAGACATAGGCTCCCTGCTGAGCAAGGATCCTCTGGTGGGACTCCA72480.15962289531442497No Hit
CCTCTGACCTCTCCATTCTCACACTCTCTCTCTCAAATAAATAAATAAAA71980.15852174399465105No Hit
TCCTCTGACCTCTCCATTCTCACACTCTCTCTCTCAAATAAATAAATAAA71980.15852174399465105No Hit
CTCCCCGCCGAACAGGGAGCCTGACGTAGGACTCGGTCCCAGGACTCCAG71410.1572664314901088No Hit
GAGAGAGTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGA69610.15330228673892274No Hit
AAAGAAATGAGGGGCGCCTGGGTGGCTCAGTTGGTTAAGCCGCTGCAGCT69040.1520469742343805No Hit
GCTCAGTTGGTTGAGCAACTGCCTTTGGCTCAGGTCATGATCCTGGAGTC68770.15145235252170258No Hit
GGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGGAGCCTGACGT67350.1483250827735447No Hit
GTTGAGCAACTGCCTTTGGCTCAGGTCATGATCCTGGAGTCCTGGGACCG66270.14594659592283307No Hit
GAAAGAAAGAAAGAAATGAGGGGCGCCTGGGTGGCTCAGTTGGTTAAGCC65920.14517578999899133No Hit
CTGCTGAGCAAGGATCCTCTGGTGGGACTCCATCTCCAGACCCTGAGATC65450.14414070775840387No Hit
GAAAGAAAGAAATGAGGGGCGCCTGGGTGGCTCAGTTGGTTAAGCCGCTG65220.14363417815130788No Hit
GGGAGACATAGGCTCCCTGCTGAGCAAGGATCCTCTGGTGGGACTCCATC65080.1433258557817712No Hit
GAAAGAAATGAGGGGCGCCTGGGTGGCTCAGTTGGTTAAGCCGCTGCAGC64420.14187233603966964No Hit
CCTCTGGTGGGACTCCATCTCCAGACCCTGAGATCATGACCCGAGCTGCA63380.1395819412945399No Hit
GAGCAAGGATCCTCTGGTGGGACTCCATCTCCAGACCCTGAGATCATGAC62800.1383046057636022No Hit
AATGAGGGGCGCCTGGGTGGCTCAGTTGGTTAAGCCGCTGCAGCTCGGGT61930.1363886024671956No Hit
GAGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGGAGCC60390.13299705640229198No Hit
GGCTTAACCAACTGAGCCACCCAGGCGCCCCTCATTTCTTTCTTTCTTTC60320.13284289521752365No Hit
GAACGTGTCAGGGAAAACTGGCTGTGAGCTTTTATGTCAGTCAAGAAAGC59690.1314554445546085No Hit
GGGTGGCTCAGTTGGTTAAGCCGCTGCAGCTCGGGTCATGATCTCAGGGT58660.12918707283587427No Hit
GTCAAGAAAGCAGAAATGAGGGGCACCTGGGTGGCTCAGTTGGTTGAGCA57690.12705083927551292No Hit
AGCGAAAGCAGGCATAGTGGCAGGCAGAAGCAGAGGGAGACATAGGCTCC56590.12462830637201032No Hit
GATCATGACCCGAGCTGCAGCGGCTTAACCAACTGAGCCACCCAGGCGCC56540.12451819124003292No Hit
GCAAAGCTCACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTTTTATGT56250.12387952347456407No Hit
GAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGGAGCCTG54790.12066416162082427No Hit
ACGTCAGGCTCCCTGTTCGGCGGGGAGTCTGCGCTTCCTCTGACCTCTCC53320.11742677674068899No Hit
GCGCAGACTCCCCGCCGAACAGGGAGCCTGACGTAGGACTCGGTCCCAGG52380.11535661225951406No Hit
AACTGGCTGTGAGCTTTTATGTCAGTCAAGAAAGCAGAAATGAGGGGCAC51960.11443164515090398No Hit
GAGAGTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAAC51630.1137048852798532No Hit
CAGTTGGTTGAGCAACTGCCTTTGGCTCAGGTCATGATCCTGGAGTCCTG51380.11315430961996624No Hit
AGTCAAGAAAGCAGAAATGAGGGGCACCTGGGTGGCTCAGTTGGTTGAGC50010.11013715500378576No Hit
GGTGGGACTCCATCTCCAGACCCTGAGATCATGACCCGAGCTGCAGCGGC49790.10965264842308525No Hit
GAGCTGCAGCGGCTTAACCAACTGAGCCACCCAGGCGCCCCTCATTTCTT49590.10921218789517569No Hit
GGCTCAGGTCATGATCCTGGAGTCCTGGGACCGAGTCCTACGTCAGGCTC48900.10769259907388769No Hit
AGGGAAAACTGGCTGTGAGCTTTTATGTCAGTCAAGAAAGCAGAAATGAG48610.10705393130841882No Hit
ACCCAGGCGCCCCTCATTTCTTTCTTTCTTTCTTTCTTTTTTTTTTTTTA48400.1065914477541138No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG47590.10480758261608007No Hit
GAAGCAGAGGGAGACATAGGCTCCCTGCTGAGCAAGGATCCTCTGGTGGG47140.10381654642828357No Hit
CTGCAGCTCGGGTCATGATCTCAGGGTCTGGAGATGGAGTCCCACCAGAG46930.10335406287397851No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA46870.10322192471560565No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA46230.10181245102629506No Hit
CAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTTTTATGTCAGTCAAGAA45970.10123985234001262No Hit
CTCTCTCTCAAATAAATAAATAAAATTTAAAAAAAAAAAAAGAAAGAAAG45820.10090950694408045No Hit
GCTCAGTTGGTTAAGCCGCTGCAGCTCGGGTCATGATCTCAGGGTCTGGA45780.10082141483849855No Hit
CAACTGAGCCACCCAGGTGCCCCTCATTTCTGCTTTCTTGACTGACATAA45700.10064523062733471No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT45440.1000726319410523No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)