FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_D7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_D7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4359939
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAGGATGGGTGGGAGGCACCAGCTCCAGGCCAGCAGGGCAGCGAGGA304440.6982666500609297No Hit
GTCCTAGACTCTTTATTTATTTGCTTATTTATTTTTTGAGGTAGAGTCTC288920.6626698217566805No Hit
GGAATAAGCAGGTCAGAAGCTCATCCTGTGATGGAGCAGGCTAGGATGGG235810.5408561908778999No Hit
ATTCTGGATATGACCATTTTCTGCACAGCCACCATGTGCCTGATAGAGAA170030.38998252039764775No Hit
GTCTTGGTGAGAGCAGAGAGCACTCCATTCTGGATATGACCATTTTCTGC161940.3714272149220436No Hit
GTTCATAGGATGTACGACTCAAGGCCGCAGCAGGGGAGGATAGGAGCCTA142140.32601373551327206No Hit
GGTCAGAAGCTCATCCTGTGATGGAGCAGGCTAGGATGGGTGGGAGGCAC131310.3011739384427167No Hit
GCCACCATGTGCCTGATAGAGAAGCACTCTAGGCTCCTATCCTCCCCTGC128540.294820638545631No Hit
CTCTAGGCTCCTATCCTCCCCTGCTGCGGCCTTGAGTCGTACATCCTATG128320.294316044329978No Hit
GCACACACCTGTCCCCAATGCCTGAGACCTGCACACACCTGTCCCTGCTA113380.2600495098669958No Hit
CACCTGTCCCTGCTATCAAAGACCTGCATACACCTCTCCTCACTGCCTGA112200.25734304998303875No Hit
GGGGAGGATGGACCCCTCACCCCCTCTGCTGCAGAGGCCAGGTCTTGGTG110530.2535127211642181No Hit
GGACAGATGTGTGCATGCAGGTCTCAGGCACTGAGAACAGGTGTGTGCAC106770.24488874729669385No Hit
GGCTGGCACTGCACATCGGGCTGGGAGGATGAACCCTGACCCAGGGCAAG103600.2376180033711481No Hit
GTGTATGCACATCTCTGATAGCAGGGACAGGTTTGTGCATGTCTCAGGCA102140.23426933266726896No Hit
GTACATCACATAGTGACAGTGGGAGCAAGAGAGAGAGATAAGGGGTAGGT100340.23014083453919884No Hit
CTGTTACCCAGGCTGGAGTGCAGTGGCATGATCTTGGCTCACGGCAACCT98100.22500314797982268No Hit
GGATGGACCCCTCACCCCCTCTGCTGCAGAGGCCAGGTCTTGGTGAGAGC97850.22442974546203515No Hit
GCACACACCTGTCCCTGCTATCAAAGACCTGCATACACCTCTCCTCACTG93940.21546173008383834No Hit
CTGCTGCAGAGGCCAGGTCTTGGTGAGAGCAGAGAGCACTCCATTCTGGA93320.2140396918397253No Hit
CCTATGAACTCAGCTCCCCTCTTCCTGCCCTTGCCCTGGGTCAGGGTTCA92140.2113332319557682No Hit
CATAGTGACAGTGGGAGCAAGAGAGAGAGATAAGGGGTAGGTCCTAGACT91190.20915430238817564No Hit
GGCCAGGTCTTGGTGAGAGCAGAGAGCACTCCATTCTGGATATGACCATT90000.20642490640350705No Hit
GATGTGTGCATGCAGGTCTCAGGCACTGAGAACAGGTGTGTGCACGTCTC89680.205690951180739No Hit
GTGATGGAGCAGGCTAGGATGGGTGGGAGGCACCAGCTCCAGGCCAGCAG88670.20337440500887743No Hit
CTTTATTTATTTGCTTATTTATTTTTTGAGGTAGAGTCTCACTCTGTTAC87040.1996358205929028No Hit
GTCTAGGACCTACCCCTTATCTCTCTCTCTTGCTCCCACTGTCACTATGT84860.19463575063779562No Hit
GCATACACCTCTCCTCACTGCCTGAGACATGCACAAACCTGTCCCTGCTA84110.19291554308443307No Hit
GTCTCACTCTGTTACCCAGGCTGGAGTGCAGTGGCATGATCTTGGCTCAC80480.1845897385261583No Hit
CCTGTAGTCTCAACTACTTGGGAAGCTGAGGGAGGAGAATCACTTCAACC79020.18124106782227917No Hit
TCCTGTGATGGAGCAGGCTAGGATGGGTGGGAGGCACCAGCTCCAGGCCA77550.17786946101768855No Hit
CTTCCTGCCCTTGCCCTGGGTCAGGGTTCATCCTCCCAGCCCGATGTGCA77080.17679146428424802No Hit
CTCCTATCCTCCCCTGCTGCGGCCTTGAGTCGTACATCCTATGAACTCAG75420.1729840715661389No Hit
ACCCTGTCTCTACTAAAAATACAAAAATTAGGCGGGTGTGGTGGTCCACG74590.17108037520708433No Hit
ACTCAAGGCCGCAGCAGGGGAGGATAGGAGCCTAGAGTGCTTCTCTATCA74180.17013999507791278No Hit
CATGGTGGCTGTGCAGAAAATGGTCATATCCAGAATGGAGTGCTCTCTGC73300.16812161821530072No Hit
GGGTAGGGTCCGCCATCAGGAGCTTAGTGGAGAGACCATTGTGCAACATG72090.1653463500292091No Hit
CCCTGACCCAGGGCAAGGGCAGGAAGAGGGGAGCTGAGTTCATAGGATGT71440.16385550348296157No Hit
GGATAGGAGCCTAGAGTGCTTCTCTATCAGGCACATGGTGGCTGTGCAGA71310.16355733417371204No Hit
CCCCCTCTGCTGCAGAGGCCAGGTCTTGGTGAGAGCAGAGAGCACTCCAT71250.16341971756944307No Hit
GAGCAGAGAGCACTCCATTCTGGATATGACCATTTTCTGCACAGCCACCA71170.16323622876375105No Hit
AGCCTAGAGTGCTTCTCTATCAGGCACATGGTGGCTGTGCAGAAAATGGT67870.1556673155289558No Hit
GATGAACCCTGACCCAGGGCAAGGGCAGGAAGAGGGGAGCTGAGTTCATA67670.15520859351472577No Hit
CCATTCTGGATATGACCATTTTCTGCACAGCCACCATGTGCCTGATAGAG66630.1528232390407297No Hit
ATCTTGGCTCACGGCAACCTCTGCCTCCCAGGTTGAAGTGATTCTCCTCC66360.15220396432151917No Hit
GGGTAACAGAGTGAGACTCTACCTCAAAAAATAAATAAGCAAATAAATAA66220.15188285891155817No Hit
GGCAGGAAGAGGGGAGCTGAGTTCATAGGATGTACGACTCAAGGCCGCAG65980.15133239249448216No Hit
GGATATGACCATTTTCTGCACAGCCACCATGTGCCTGATAGAGAAGCACT65450.15011677915677263No Hit
GTCCACGCCTGTAGTCTCAACTACTTGGGAAGCTGAGGGAGGAGAATCAC64520.14798372179060304No Hit
AGCCAGTACATCACATAGTGACAGTGGGAGCAAGAGAGAGAGATAAGGGG64110.14704334166143151No Hit
GCCTGATAGAGAAGCACTCTAGGCTCCTATCCTCCCCTGCTGCGGCCTTG63810.14635525864008647No Hit
AAGCAGGGGAGGATGGACCCCTCACCCCCTCTGCTGCAGAGGCCAGGTCT63350.14530019800735744No Hit
CACCTGTCCCCAATGCCTGAGACCTGCACACACCTGTCCCTGCTATCAAA63120.14477266769099292No Hit
GGTGTGTGCTGGTCTCAGGCAGGGGGACAGGTGCGTGCAGGTCTCAGGCA61750.14163042189351732No Hit
TTTCTGCACAGCCACCATGTGCCTGATAGAGAAGCACTCTAGGCTCCTAT61560.14119463597999882No Hit
GGTGTATGCAGGTCTTTGATAGCAGGGACAGGTGTGTGCAGGTCTCAGGC61500.14105701937572981No Hit
ATGTACGACTCAAGGCCGCAGCAGGGGAGGATAGGAGCCTAGAGTGCTTC61470.1409882110735953No Hit
GCCTTGAGTCGTACATCCTATGAACTCAGCTCCCCTCTTCCTGCCCTTGC61400.1408276583686148No Hit
ACCCTGACCCAGGGCAAGGGCAGGAAGAGGGGAGCTGAGTTCATAGGATG60660.13913038691596372No Hit
CTCTGCTGCAGAGGCCAGGTCTTGGTGAGAGCAGAGAGCACTCCATTCTG60510.13878634540529122No Hit
GTTTGAGACCAGCCTGGCCAACATGGCGAAACCCTGTCTCTACTAAAAAT59440.13633218262916064No Hit
GAACAGGTGTGTGCACGTCTCAGGAAGAGGGACAAGTGTATGCACATCTC58790.13484133608291307No Hit
GAGTTCATAGGATGTACGACTCAAGGCCGCAGCAGGGGAGGATAGGAGCC58690.13461197507579808No Hit
GTCTCAGGCAGTGAGGAGAGGTGTATGCAGGTCTTTGATAGCAGGGACAG58310.13374040324876105No Hit
CGCCTGTAGTCTCAACTACTTGGGAAGCTGAGGGAGGAGAATCACTTCAA57700.1323413011053595No Hit
CCTCACCCCCTCTGCTGCAGAGGCCAGGTCTTGGTGAGAGCAGAGAGCAC57190.13117155996907295No Hit
ATCCAGAATGGAGTGCTCTCTGCTCTCACCAAGACCTGGCCTCTGCAGCA56970.13066696575341996No Hit
GCCTGAGACATGCACAAACCTGTCCCTGCTATCAGAGATGTGCATACACT56270.1290614387036149No Hit
CCATGTGCCTGATAGAGAAGCACTCTAGGCTCCTATCCTCCCCTGCTGCG55350.1269513174381568No Hit
TCTCAGGAAGAGGGACAAGTGTATGCACATCTCTGATAGCAGGGACAGGT54560.12513936548194826No Hit
GATAAGGGGTAGGTCCTAGACTCTTTATTTATTTGCTTATTTATTTTTTG54450.12488706837412175No Hit
GAAGAGGGGAGCTGAGTTCATAGGATGTACGACTCAAGGCCGCAGCAGGG54370.12470357956842974No Hit
GTGTGGTGGTCCACGCCTGTAGTCTCAACTACTTGGGAAGCTGAGGGAGG54250.12442834635989174No Hit
GGCTGGGAGGATGAACCCTGACCCAGGGCAAGGGCAGGAAGAGGGGAGCT53910.12364851893570071No Hit
GGGCAAGGGCAGGAAGAGGGGAGCTGAGTTCATAGGATGTACGACTCAAG53740.1232586052236052No Hit
CCCCTCACCCCCTCTGCTGCAGAGGCCAGGTCTTGGTGAGAGCAGAGAGC51900.11903836269268905No Hit
ACATCACATAGTGACAGTGGGAGCAAGAGAGAGAGATAAGGGGTAGGTCC51590.11832734357063253No Hit
ATCCTCCCCTGCTGCGGCCTTGAGTCGTACATCCTATGAACTCAGCTCCC51050.11708879413221149No Hit
GTCGTACATCCTATGAACTCAGCTCCCCTCTTCCTGCCCTTGCCCTGGGT49420.11335020971623687No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC49040.11247863788919983No Hit
CCCCTGCTGCGGCCTTGAGTCGTACATCCTATGAACTCAGCTCCCCTCTT48860.11206578807639281No Hit
GAGTGAGACTCTACCTCAAAAAATAAATAAGCAAATAAATAAAGAGTCTA48340.11087311083939476No Hit
GCACTGCACATCGGGCTGGGAGGATGAACCCTGACCCAGGGCAAGGGCAG48250.11066668593299127No Hit
AGCTCATCCTGTGATGGAGCAGGCTAGGATGGGTGGGAGGCACCAGCTCC47760.10954281699812772No Hit
CTCCAGGCCAGCAGGGCAGCGAGGAGGCTGGCACTGCACATCGGGCTGGG47710.10942813649457023No Hit
AGCGAAAGCAGGGGAGGATGGACCCCTCACCCCCTCTGCTGCAGAGGCCA47580.10912996718532073No Hit
GGATGTACGACTCAAGGCCGCAGCAGGGGAGGATAGGAGCCTAGAGTGCT47500.1089464783796287No Hit
CTATCAGGCACATGGTGGCTGTGCAGAAAATGGTCATATCCAGAATGGAG47470.1088776700774942No Hit
AATTAGGCGGGTGTGGTGGTCCACGCCTGTAGTCTCAACTACTTGGGAAG47380.10867124517109071No Hit
GCTCCAGGCCAGCAGGGCAGCGAGGAGGCTGGCACTGCACATCGGGCTGG47010.10782260944476516No Hit
ATAGAGAAGCACTCTAGGCTCCTATCCTCCCCTGCTGCGGCCTTGAGTCG47010.10782260944476516No Hit
GTGGTGGTCCACGCCTGTAGTCTCAACTACTTGGGAAGCTGAGGGAGGAG46450.10653818780492112No Hit
GTGCCAGCCTCCTCGCTGCCCTGCTGGCCTGGAGCTGGTGCCTCCCACCC46270.1061253379921141No Hit
GAGAACAGGTGTGTGCACGTCTCAGGAAGAGGGACAAGTGTATGCACATC45900.10527670226578859No Hit
GTGCAGGTCTCTGCTATCAGAGACCTGCACACACCTGTCCCCAATGCCTG45570.10451981094230905No Hit
ATCAAAGACCTGCATACACCTCTCCTCACTGCCTGAGACATGCACAAACC45460.10426751383448254No Hit
CCTGTCCCCAATGCCTGAGACCTGCACACACCTGTCCCTGCTATCAAAGA44950.103097772698196No Hit
GCACATCGGGCTGGGAGGATGAACCCTGACCCAGGGCAAGGGCAGGAAGA44460.10197390376333246No Hit
GTGACAGTGGGAGCAAGAGAGAGAGATAAGGGGTAGGTCCTAGACTCTTT44010.10094177923131495No Hit
GACCTGCACACACCTGTCCCTGCTATCAAAGACCTGCATACACCTCTCCT43670.10016195180712392No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)