FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_D6a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_D6a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences991098
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATTGCTGTGCATGATGGAGCGCACTGGCAAGCACTACAGCATTTTG57270.5778439669941823No Hit
GTCCAGGTCAGAAAGGGCCAGTGCCAGGGAGAACTGAAAGGAGGGAACAG43040.43426583445834827No Hit
GTAGGAGGAAGGGAGTGGAGAAAGAAGAGAGGAATCAAAGGCCACGGGGG36110.3643433848115928No Hit
GCCCTAGGTTTTGGCCTCAGCCACTGGGAAGATGGTGATGGCATTTACTG34540.348502368080654No Hit
CTACAGCATTTTGAATAATTAGTGTCAGCCATTCTTCCTGTCCTCTTGCA30110.3038044673685145No Hit
TCCTCCTACTGTCTGCTTCCCCATAAGTCCTGGGGCTCTAGTGTCTGTTC29070.2933110550117143No Hit
GTATTCAGTAAATGCCATCACCATCTTCCCAGTGGCTGAGGCCAAAACCT28770.2902841091395604No Hit
GTGCCAGGGAGAACTGAAAGGAGGGAACAGACACTAGAGCCCCAGGACTT28060.2831203372421294No Hit
CAGTAGGAGGAAGGGAGTGGAGAAAGAAGAGAGGAATCAAAGGCCACGGG25530.2575930937202981No Hit
ACATAGTTCAGTCTGTATTCAGTAAATGCCATCACCATCTTCCCAGTGGC24650.24871405249531328No Hit
GCAATACATAGTTCAGTCTGTATTCAGTAAATGCCATCACCATCTTCCCA24250.24467812466577474No Hit
ACTGAATACAGACTGAACTATGTATTGCTGTGCATGATGGAGCGCACTGG22730.22934159891352823No Hit
GAATTGGATGGGCGTAGTAGGTATTTTGGAAGGTGAACAGATGGGGCTTA22360.22560836567120504No Hit
GAACTATGTATTGCTGTGCATGATGGAGCGCACTGGCAAGCACTACAGCA22050.2224805216033127No Hit
ACCTAGGGCTCATCCTCAAATCCTCTGCTGCCATGCAACCCTCTTCAGTA21330.21521585151014327No Hit
GGCTGGGACTGGGGGGCCGAGGCCGAGAAACACGCAGCGGGGCTGGGCAG20790.20976734894026625No Hit
ATGCTGTAGTGCTTGCCAGTGCGCTCCATCATGCACAGCAATACATAGTT20410.20593321750220464No Hit
GGCAAGCACTACAGCATTTTGAATAATTAGTGTCAGCCATTCTTCCTGTC20380.20563052291498923No Hit
GGTCAGAAAGGGCCAGTGCCAGGGAGAACTGAAAGGAGGGAACAGACACT18490.18656076392041956No Hit
CCCCAGGACTTATGGGGAAGCAGACAGTAGGAGGAAGGGAGTGGAGAAAG18310.18474459639712723No Hit
ATCAAAGGCCACGGGGGTTTTAACCTGCAAGAGGACAGGAAGAATGGCTG17730.17889250104429633No Hit
GTGCATGATGGAGCGCACTGGCAAGCACTACAGCATTTTGAATAATTAGT17690.17848890826134248No Hit
CACTAGAGCCCCAGGACTTATGGGGAAGCAGACAGTAGGAGGAAGGGAGT16760.16910537605766535No Hit
GCATGATGGAGCGCACTGGCAAGCACTACAGCATTTTGAATAATTAGTGT16360.1650694482281268No Hit
GAGGAAGGGAGTGGAGAAAGAAGAGAGGAATCAAAGGCCACGGGGGTTTT16030.1617398077687575No Hit
TTACTGAAGAGGGTTGCATGGCAGCAGAGGATTTGAGGATGAGCCCTAGG15910.1605290294198959No Hit
CCCTGGCACTGGCCCTTTCTGACCTGGACTGTGGCAAAGGCTCCGGTGCA15570.15709849076478816No Hit
CTCCTACTGTCTGCTTCCCCATAAGTCCTGGGGCTCTAGTGTCTGTTCCC15250.1538697485011573No Hit
GACCTGGACTGTGGCAAAGGCTCCGGTGCAAGTCTCGCTGCCCAGCCCCG14960.15094370082474184No Hit
GGGCTGGGCAGCGAGACTTGCACCGGAGCCTTTGCCACAGTCCAGGTCAG14770.14902663510571104No Hit
ATCATGCACAGCAATACATAGTTCAGTCTGTATTCAGTAAATGCCATCAC14360.14488980908043403No Hit
CTAGAGCCCCAGGACTTATGGGGAAGCAGACAGTAGGAGGAAGGGAGTGG14140.14267004877418782No Hit
TAATTAGTGTCAGCCATTCTTCCTGTCCTCTTGCAGGTTAAAACCCCCGT13610.13732244440004923No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT13370.1349008877023261No Hit
CCCTTCCTCCTACTGTCTGCTTCCCCATAAGTCCTGGGGCTCTAGTGTCT13060.13177304363443373No Hit
GGGTTGCATGGCAGCAGAGGATTTGAGGATGAGCCCTAGGTTTTGGCCTC12970.13086495987278754No Hit
GTGCTTGCCAGTGCGCTCCATCATGCACAGCAATACATAGTTCAGTCTGT12870.12985597791540293No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA12720.12834250497932595No Hit
GGATTTGAGGATGAGCCCTAGGTTTTGGCCTCAGCCACTGGGAAGATGGT12520.12632454106455668No Hit
GCACTACAGCATTTTGAATAATTAGTGTCAGCCATTCTTCCTGTCCTCTT12460.1257191518901259No Hit
CTGTAGTGCTTGCCAGTGCGCTCCATCATGCACAGCAATACATAGTTCAG12460.1257191518901259No Hit
GTTTTGGCCTCAGCCACTGGGAAGATGGTGATGGCATTTACTGAATACAG12210.1231966969966643No Hit
GTATTGCTGTGCATGATGGAGCGCACTGGCAAGCACTACAGCATTTTGAA12180.12289400240944892No Hit
ACTGAAGAGGGTTGCATGGCAGCAGAGGATTTGAGGATGAGCCCTAGGTT12000.12107783488615657No Hit
GTTTTAACCTGCAAGAGGACAGGAAGAATGGCTGACACTAATTATTCAAA11890.11996795473303347No Hit
GAAAGGGCCAGTGCCAGGGAGAACTGAAAGGAGGGAACAGACACTAGAGC11860.11966526014581806No Hit
GGTTAAAACCCCCGTGGCCTTTGATTCCTCTCTTCTTTCTCCACTCCCTT11760.11865627818843344No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC11520.11623472149071029No Hit
GGTTTTAACCTGCAAGAGGACAGGAAGAATGGCTGACACTAATTATTCAA11460.11562933231627952No Hit
ATTCAAAATGCTGTAGTGCTTGCCAGTGCGCTCCATCATGCACAGCAATA11260.11361136840151023No Hit
GACTTGCACCGGAGCCTTTGCCACAGTCCAGGTCAGAAAGGGCCAGTGCC11090.11189609907395635No Hit
ATTTTGAATAATTAGTGTCAGCCATTCTTCCTGTCCTCTTGCAGGTTAAA10990.11088711711657172No Hit
GATGGGGCTTACTGAAGAGGGTTGCATGGCAGCAGAGGATTTGAGGATGA10980.11078621892083326No Hit
GAAGAGGGTTGCATGGCAGCAGAGGATTTGAGGATGAGCCCTAGGTTTTG10960.11058442252935632No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG10770.10866735681032552No Hit
GAAGAGAGGAATCAAAGGCCACGGGGGTTTTAACCTGCAAGAGGACAGGA10740.10836466222311013No Hit
GTATTTTGGAAGGTGAACAGATGGGGCTTACTGAAGAGGGTTGCATGGCA10740.10836466222311013No Hit
GTCCTGGGGCTCTAGTGTCTGTTCCCTCCTTTCAGTTCTCCCTGGCACTG10640.10735568026572549No Hit
TTTCTGACCTGGACTGTGGCAAAGGCTCCGGTGCAAGTCTCGCTGCCCAG10640.10735568026572549No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA10560.10654849469981778No Hit
CCATCATGCACAGCAATACATAGTTCAGTCTGTATTCAGTAAATGCCATC10490.10584220732964852No Hit
GGCGTAGTAGGTATTTTGGAAGGTGAACAGATGGGGCTTACTGAAGAGGG10460.10553951274243314No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA10420.1051359199594793No Hit
GGCTTACTGAAGAGGGTTGCATGGCAGCAGAGGATTTGAGGATGAGCCCT10380.10473232717652543No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT10310.10402603980635618No Hit
CCATCTTCCCAGTGGCTGAGGCCAAAACCTAGGGCTCATCCTCAAATCCT10180.10271436326175615No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)