FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_D4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_D4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11994422
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATGGTGCCTCCATACATGGAAATTGGCAGACCTTTAGAAAAATCC520380.4338516687173421No Hit
CCATAATACCCTCCCATCCTCAGCTTGCTGATGTTCCAGGTGGACCTCTG391390.3263100131044247No Hit
GATTAGGAGAGGCCACCATTGGCTGTCCAACTCTCTTCTTCGGTCTGGTT357910.29839703822326746No Hit
CCATAAGGGGTGGTACCGAATCAACAAGATATGCAGTTCAACTTATCAAT297710.24820704157315793No Hit
CATATATCCTTAGCACAGCAAAAAGCTGATAAAAATAAAATAAATGGAGA267060.22265349676708054No Hit
CAGTGGGATCTGGTCATTTGGAGTCAACGCTATGTCAGAAGGCTTATCAG264430.220460810866918No Hit
GAGTAGCAGTGACGGGTATCTCCTATCATTAGAGGAATTCACATCCATAG260980.21758447384959442No Hit
ATCACAGACTGTGACCAACCTGATAAGCCTTCTGACATAGCGTTGACTCC259050.21597539256164242No Hit
GACTTAAAGCTTGTTGATAAGGAATTAGAATTCGTTTCACCTTCAAGTCG256860.2141495438462979No Hit
CTGCTACTCCATTCCCAAGAAGAAAAGACAAGTACTGCTACTTCCAAAAC255590.213090718335573No Hit
GTCTGTGATAGGGAACCATCCAATGCATCAACAATTATCAGACCCAAGCA245690.20483688167716624No Hit
ACCCAGAGGTGGTGGTACAGGTGGGAATAGTGACTCGGATAACTTGGACA233460.19464047538097293No Hit
CTCCTAGCACCAATCGGAAAAATAAGAAGAACAAAACAAAGGAGACCCCT232200.19358998707899389No Hit
CCCTTATGGTGATCATTCTCTCGCCGTTCTTGTCTTTTTGTTTATCCACA230980.1925728476119983No Hit
GTATAGCAGTGATGTTGCACCCTCCCCTTCCCATTATCCTGGAAACCACG192420.16042457068794144No Hit
GGATATATGTTGTTCTGGTAATATTAAATGTGCAGTGGGAGGGGTCTCCT189510.1579984429428946No Hit
ACATATATCCTTAGCACAGCAAAAAGCTGATAAAAATAAAATAAATGGAG183420.1529210828166626No Hit
TCCTTGGACAAGCGCCTCAAACTCATCCACCTCTGCCCCACCAACTTTGG182630.15226244332573924No Hit
ATACAAGACGTTACGGGTGCGCATATTGATGTGGATAAACAAAAAGACAA176510.14716007157326963No Hit
CCTTAGCACAGCAAAAAGCTGATAAAAATAAAATAAATGGAGAACCCAGA175640.14643473441237934No Hit
CTGCTATACAAGACGTTACGGGTGCGCATATTGATGTGGATAAACAAAAA172590.14389188574489042No Hit
GAGTAGCAGTGGTGGTAAAAGCCAAGAGTTAAATTTCACTATGGATGTGA161220.1344124794008415No Hit
ACCTATGGCAAGCAGTTTTCTGGATTTTTCTAAAGGTCTGCCAATTTCCA159350.13285342136536465No Hit
CCCAAAGGAAATGCATTTTTACTGGAAGCTGCTGTGGTGCCAACTCCAGA157590.13138607262609237No Hit
GTGCTAGGAGTTGTCACCACATTGGCCCTTTTTTTCCCAAACACATTTGT156330.13033558432411332No Hit
CCTCTGGGTTCTCCATTTATTTTATTTTTATCAGCTTTTTGCTGTGCTAA154190.12855142165249814No Hit
CTGCTACTCTCACTGTTGCAGTCTGTGCTGTCCAAGTTATCCGAGTCACT152630.12725081708814312No Hit
GGTGGGAATAGTGACTCGGATAACTTGGACAGCACAGACTGCAACAGTGA149720.1248246893430963No Hit
GCACAGACTGCAACAGTGAGAGTAGCAGTGGTGGTAAAAGCCAAGAGTTA149720.1248246893430963No Hit
ATGTTGCACCCTCCCCTTCCCATTATCCTGGAAACCACGGAAGCTGGAAC148820.12407434055596843No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG146990.12254863135547507No Hit
GACTGCAACAGTGAGAGTAGCAGTGGTGGTAAAAGCCAAGAGTTAAATTT145660.12143978259227498No Hit
GTCTTGTATAGCAGTGATGTTGCACCCTCCCCTTCCCATTATCCTGGAAA140190.11687932940828662No Hit
GTTGCAGTCTGTGCTGTCCAAGTTATCCGAGTCACTATTCCCACCTGTAC136010.11339437615251491No Hit
AACTTGGACAGCACAGACTGCAACAGTGAGAGTAGCAGTGGTGGTAAAAG134480.11211878321439749No Hit
TCCTAGCACCAATCGGAAAAATAAGAAGAACAAAACAAAGGAGACCCCTC134420.1120687599619223No Hit
CCCCTGTGGGGACCAGTTTTGTTGCTCCTGTTGGACACAGTGGGATCTGG132760.11068478331010866No Hit
ATTCCCACCTGTACCACCACCTCTGGGTTCTCCATTTATTTTATTTTTAT132070.11010951590664393No Hit
TCCTTTGGGTGCTCCAGCTCTCGTAACATCACAGGCGACAACATTATCAA131610.10972600430433413No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA130430.10874221367232202No Hit
CATTGGAACAGAGAGGCTAGCCCGGATTCGGCAAGGAGGATCTGTCGCAC130410.10872553925483029No Hit
ATCCTTAGCACAGCAAAAAGCTGATAAAAATAAAATAAATGGAGAACCCA129380.10786680675400614No Hit
ATCCTGGAAACCACGGAAGCTGGAACGGACAACTTCTTTGACCTTCGTAC128550.10717481842809932No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT127050.10592423711621954No Hit
CCCGTAACGTCTTGTATAGCAGTGATGTTGCACCCTCCCCTTCCCATTAT126080.10511552786787058No Hit
CATTAGAGGAATTCACATCCATAGTGAAATTTAACTCTTGGCTTTTACCA125380.10453192325566002No Hit
GCCATAGGTTGATGAAAAATGTTAGAGGGGGCTACCATTCCAGAATCATC123380.10286448150648693No Hit
TCTCTTTTCTGTCCCCTTTTGGGGCTAGTTAGATTCAGCTTTATGTTTGG122900.10246429548668538No Hit
GTACAGAGTTACCCAGGAACGCTGAATTTGCTGGAACGTTCTTGTTAAGT122640.1022475280592929No Hit
GTGCAACATCACTGCTATACAAGACGTTACGGGTGCGCATATTGATGTGG122390.10203909784064626No Hit
GTTGGACACAGTGGGATCTGGTCATTTGGAGTCAACGCTATGTCAGAAGG121940.10166392344708232No Hit
GTCCAAGGATGGGGGGAAAAATGTTGCCTGTTGAAACTTGGAGTCCCAAC121720.10148050485467328No Hit
GTGCTAAGGATATATGTTGTTCTGGTAATATTAAATGTGCAGTGGGAGGG121590.10137212114097703No Hit
CCATTAAACAGAGGTCCACCTGGAACATCAGCAAGCTGAGGATGGGAGGG120330.10032163283899799No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)