FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_D3a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_D3a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1453578
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTGATCTGCCTATCTGCGTACATACATCGATAGTATTCTGAAAGCAA100410.6907782038528376No Hit
AGTAATAACAGCACGTATTTATTAAGGGACTACTACATTCCACATCCTGT82730.56914730410064No Hit
GATATATCTCCATATATATTGATCTGCCTATCTGCGTACATACATCGATA77460.5328919397514272No Hit
GTTATTACTATGGTGCAAAAATCATCTTGCTGGGTGGAATACAGGTACTA76850.5286953985269452No Hit
TTATGATACTGGACAGCAAGCAAACCTGCCTTTGTGCTCTGGAGAGAGAC73270.5040665172422808No Hit
GTACAGGGAGGCACTAGTACCTGTATTCCACCCAGCAAGATGATTTTTGC69240.47634182685758863No Hit
CCATATATATTGATCTGCCTATCTGCGTACATACATCGATAGTATTCTGA62680.43121180975496326No Hit
GCCTATCTGCGTACATACATCGATAGTATTCTGAAAGCAATGGGGATCTG49280.33902549433191753No Hit
TCCCTGTACCGACCCTGTCCCTGCATGTCCTTCCAGCACAGGCATTGGTG49140.33806235372302No Hit
CTTCTAAGACTGTTTGCTAAAAATCACCTTGAAGGGGTAATTCAGAACAA45640.3139838385005827No Hit
GTCTTAATGCTTTAGCTGTTTTAACTCTTTTGGTCCCTCATACGTGAGAG44520.3062787136294028No Hit
ATACTGGACAGCAAGCAAACCTGCCTTTGTGCTCTGGAGAGAGACATGAT42650.29341390692484337No Hit
GTTGATTACCTGTTAGAGCCTCTCACGTATGAGGGACCAAAAGAGTTAAA41540.2857775778114418No Hit
TTCTAAGACTGTTTGCTAAAAATCACCTTGAAGGGGTAATTCAGAACAAA41200.2834385220469765No Hit
GTAATAACAGCACGTATTTATTAAGGGACTACTACATTCCACATCCTGTC40800.28068669173584077No Hit
CATCGATAGTATTCTGAAAGCAATGGGGATCTGTTGATTACCTGTTAGAG34040.2341807594776476No Hit
GCGTACATACATCGATAGTATTCTGAAAGCAATGGGGATCTGTTGATTAC33400.22977783097983046No Hit
ATAGTAATAACAGCACGTATTTATTAAGGGACTACTACATTCCACATCCT31960.21987124185974197No Hit
GTTAGAGCCTCTCACGTATGAGGGACCAAAAGAGTTAAAACAGCTAAAGC31880.21932087579751483No Hit
GTCTTAGAAGACAGAGATCATGTCTCTCTCCAGAGCACAAAGGCAGGTTT30930.21278527880856757No Hit
ATCTTTATGATACTGGACAGCAAGCAAACCTGCCTTTGTGCTCTGGAGAG30490.2097582654663183No Hit
ATATATCTCCATATATATTGATCTGCCTATCTGCGTACATACATCGATAG29710.2043921963596037No Hit
ATAGTATTCTGAAAGCAATGGGGATCTGTTGATTACCTGTTAGAGCCTCT29710.2043921963596037No Hit
ACTACATTCCACATCCTGTCTTAATGCTTTAGCTGTTTTAACTCTTTTGG29210.20095240847068405No Hit
AAACAAGGGCTTGAATTGGTTGGTTTGAACTCGTCTCAAAATACATGCAG28910.19888853573733228No Hit
GTATTCCACCCAGCAAGATGATTTTTGCACCATAGTAATAACAGCACGTA28710.19751262058176444No Hit
CTTTATGATACTGGACAGCAAGCAAACCTGCCTTTGTGCTCTGGAGAGAG27530.18939472116391415No Hit
GACAGATATATCTCCATATATATTGATCTGCCTATCTGCGTACATACATC27010.18581734175943776No Hit
CGTATGAGGGACCAAAAGAGTTAAAACAGCTAAAGCATTAAGACAGGATG27000.18574854600165935No Hit
GTACCGACCCTGTCCCTGCATGTCCTTCCAGCACAGGCATTGGTGAAATT26330.18113923023050707No Hit
CATATATATTGATCTGCCTATCTGCGTACATACATCGATAGTATTCTGAA26200.18024488537938796No Hit
GCCTTAGAGGCAGACAGGATCTTTATGATACTGGACAGCAAGCAAACCTG26170.1800384981060528No Hit
TTCCACATCCTGTCTTAATGCTTTAGCTGTTTTAACTCTTTTGGTCCCTC26030.1790753574971553No Hit
TATTACTATGGTGCAAAAATCATCTTGCTGGGTGGAATACAGGTACTAGT24800.17061347929041304No Hit
GCATTAAGACAGGATGTGGAATGTAGTAGTCCCTTAATAAATACGTGCTG24110.16586657200370397No Hit
CTGTTATTACTATGGTGCAAAAATCATCTTGCTGGGTGGAATACAGGTAC22590.15540961682138832No Hit
GGAGAGAGACATGATCTCTGTCTTCTAAGACTGTTTGCTAAAAATCACCT21650.14894281559021944No Hit
GTAGTAGTCCCTTAATAAATACGTGCTGTTATTACTATGGTGCAAAAATC21180.145709414974635No Hit
ATGTTACGTAGACAGATATATCTCCATATATATTGATCTGCCTATCTGCG21140.14543423194352142No Hit
TCTCCATATATATTGATCTGCCTATCTGCGTACATACATCGATAGTATTC20910.1438519295146184No Hit
GTAGAAACAAGGGCTTGAATTGGTTGGTTTGAACTCGTCTCAAAATACAT20750.14275119739016415No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC20240.13924261374346614No Hit
TACGTAGACAGATATATCTCCATATATATTGATCTGCCTATCTGCGTACA20030.1377979028301199No Hit
TGCAGGGACAGGGTCGGTACAGGGAGGCACTAGTACCTGTATTCCACCCA19640.13511486827676258No Hit
ATGTAGTAGTCCCTTAATAAATACGTGCTGTTATTACTATGGTGCAAAAA19530.13435811494120026No Hit
AACCTAACCATGACTTGCCTTAGAGGCAGACAGGATCTTTATGATACTGG19090.131331101598951No Hit
GACTACTACATTCCACATCCTGTCTTAATGCTTTAGCTGTTTTAACTCTT19010.13078073553672387No Hit
CCTGTATTCCACCCAGCAAGATGATTTTTGCACCATAGTAATAACAGCAC18750.12899204583448565No Hit
GGCTTGAATTGGTTGGTTTGAACTCGTCTCAAAATACATGCAGAGAACCC18550.1276161306789178No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG18450.12692817310113388No Hit
GGTGATGCCACTCGCACACTGAGTTTGTGCCAAAGCCGAAGAGCTTAGCA18380.12644660279668513No Hit
TATTAAGGGACTACTACATTCCACATCCTGTCTTAATGCTTTAGCTGTTT18120.12465791309444695No Hit
CTACTACATTCCACATCCTGTCTTAATGCTTTAGCTGTTTTAACTCTTTT18070.12431393430555497No Hit
CCATAGTAATAACAGCACGTATTTATTAAGGGACTACTACATTCCACATC18010.12390115975888462No Hit
AACCATGACTTGCCTTAGAGGCAGACAGGATCTTTATGATACTGGACAGC17890.12307561066554393No Hit
GAATGTAGTAGTCCCTTAATAAATACGTGCTGTTATTACTATGGTGCAAA17600.12108053368997053No Hit
TTACTATGGTGCAAAAATCATCTTGCTGGGTGGAATACAGGTACTAGTGC17410.1197734142921811No Hit
GTCCCTGCATGTCCTTCCAGCACAGGCATTGGTGAAATTTTGTTCTGAAT17360.11942943550328912No Hit
TAGTAATAACAGCACGTATTTATTAAGGGACTACTACATTCCACATCCTG17280.11887906944106198No Hit
TACATCGATAGTATTCTGAAAGCAATGGGGATCTGTTGATTACCTGTTAG17260.11874147792550521No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA16910.11633362640326146No Hit
GCTCTGGAGAGAGACATGATCTCTGTCTTCTAAGACTGTTTGCTAAAAAT16620.11433854942768809No Hit
TACATACATCGATAGTATTCTGAAAGCAATGGGGATCTGTTGATTACCTG16570.11399457063879612No Hit
GCTTAGCACAGGCAGACCCAGTCTTTCCTAGCAGCCTCTGCGCAAACCTA16290.11206828942100114No Hit
GGGTAATTCAGAACAAAATTTCACCAATGCCTGTGCTGGAAGGACATGCA16280.11199949366322276No Hit
GTGGAATACAGGTACTAGTGCCTCCCTGTACCGACCCTGTCCCTGCATGT16220.1115867191165524No Hit
CACTGAGTTTGTGCCAAAGCCGAAGAGCTTAGCACAGGCAGACCCAGTCT15760.10842211425874636No Hit
GGCACGGGTGATGCCACTCGCACACTGAGTTTGTGCCAAAGCCGAAGAGC15710.10807813546985438No Hit
GAATACAGGTACTAGTGCCTCCCTGTACCGACCCTGTCCCTGCATGTCCT15640.10759656516540564No Hit
CATTAAGACAGGATGTGGAATGTAGTAGTCCCTTAATAAATACGTGCTGT15230.10477593909649155No Hit
GTTTTAACTCTTTTGGTCCCTCATACGTGAGAGGCTCTAACAGGTAATCA15140.10415677727648603No Hit
GTATTATATGGCATGAATATGGCACACTTTGTTTATCCATTCTCTAGTTG15100.10388159424537244No Hit
GGTCGGTACAGGGAGGCACTAGTACCTGTATTCCACCCAGCAAGATGATT14900.10250567908980461No Hit
TATTTATTAAGGGACTACTACATTCCACATCCTGTCTTAATGCTTTAGCT14680.10099217241867997No Hit
CACTAGTACCTGTATTCCACCCAGCAAGATGATTTTTGCACCATAGTAAT14590.10037301059867446No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)