FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_D3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_D3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2839251
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG157100.5533149411587774No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC137480.48421220948764304No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC130700.46033267224348956No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC103320.3638987888002857No Hit
GTATAAGACCTTGCTTCTGGGTTGAACTAATCAGAGGGCGACCCAAAGAG103250.3636522449054346No Hit
ACTCAGCACCGTCTGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA95300.33565190256162625No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC83470.29398598433178325No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG81650.2875758430656536No Hit
GTCCAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGGTTTTCATTCAAA75290.265175569190607No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA75070.26440071694964623No Hit
GGATACATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAA71630.25228484554553293No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC60120.21174598512072373No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA58870.20734341556981048No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA58740.20688554833651554No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA56870.2002993042883493No Hit
GCTATATCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGA55400.1951218824964753No Hit
GTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGT52630.18536578837165155No Hit
ATGTTACATTTACCCAAATGCAATGGGGCTACCCCTCTTAGTTTGCATAG52220.183921745558952No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT51990.18311167276158397No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA51700.18209027662577207No Hit
GACTGGGACAGACAATAACTTCTCAATAAAGCAAGATATCGTAGGAATAA49830.17550403257760586No Hit
GTCTAATGGAGCAAATGGAGTAAAAGGGTTTTCATTCAAATACGGCAATG47980.16898822964225424No Hit
ATCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTA47940.16884734741662502No Hit
GACAATAACTTCTCAATAAAGCAAGATATCGTAGGAATAAATGAGTGGTC46730.16458566009134099No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT46420.16349382284271452No Hit
GTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAACCGTTTCTTGA45720.16102838389420307No Hit
GGATATAGCGGGAGTTTTGTTCAGCATCCAGAACTAACAGGGCTGGATTG45300.15954912052509623No Hit
CTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTAGT44810.15782331326113824No Hit
CATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGACAG43620.15363206704866883No Hit
CTCTTAGTTTGCATAGTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACA43300.15250500924363503No Hit
ATATACATCCGATCACAATTGGAAAATGTCCAAAATATGTAAAAAGCACA41630.14662317632361493No Hit
CCCCTATGTCTCCCCATATCCCTTGTGAGGTTAGGTTTGTATACCCACAA41560.14637663242876378No Hit
GTGTTTGGATAGGGAGAACTAAAAGCATTAGTTCAAGAAACGGTTTTGAG41350.14563700074421035No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG40170.14148097508814825No Hit
TGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAAATGGAG40150.14141053397533362No Hit
GTTATTGTCTGTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAAC40070.1411287695240752No Hit
GATGTATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTT37620.1324997332042852No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA37600.13242929209147059No Hit
TACATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGAC36720.12932988312762767No Hit
CATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTATTGTCTGTCCCA36180.12742797308163314No Hit
CCCTAATGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAA35280.12425812300497562No Hit
GTCCTGGACTGAGATCTGTCAGGTTTTGCCTGGGTCCCTTTGCACCACTG35200.12397635855371715No Hit
CTCCTTGGTTGACCTAGAAAAGCCAGTGGAGCCATCCGTGTGTCCCAGAG33700.11869327509262126No Hit
ATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCGCTATATCCTG33370.11753099673118017No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA31960.11256489827775001No Hit
AGGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCTCTTTGGGTCGCCCT31930.11245923660852811No Hit
GTGTCACTGTTTACACCACAAAAGGATATGCTGCTCCCGCTAGTCCAGAT31780.11193092826241852No Hit
ATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCGCTATA31730.11175482548038196No Hit
ATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGGATTGTCTCCG31630.11140261991630891No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC31250.11006423877283128No Hit
ATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCATG31050.10935982764468516No Hit
GTTTACACCACAAAAGGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCT30970.10907806319342672No Hit
GTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTG30830.10858497540372444No Hit
GTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAA30720.10819754928324407No Hit
ATCCAAACACCATTGCCGTATTTGAATGAAAACCCTTTTACTCCATTTGC29420.10361887695029429No Hit
TCTTTATCCCGTCCCTCTGGGACACACGGATGGCTCCACTGGCTTTTCTA29060.10235093691963128No Hit
GAATAAATGAGTGGTCAGGATATAGCGGGAGTTTTGTTCAGCATCCAGAA28530.10048424743004404No Hit
GGTCAGGATATAGCGGGAGTTTTGTTCAGCATCCAGAACTAACAGGGCTG28470.10027292409160021No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)