FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_D2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_D2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences811215
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATAGATAGATAGATCTTTACTCTGGACAGTTAGGTGGGAACAGTTTT16680.2056174996764113No Hit
ATACAATCTGTACCCCGATGATTAAATGCAATTCTAGTTCTCGAACTCTT12830.15815782499090872No Hit
GTGCAGGGGTTGATCCCCCGGACCCTGGGATCATGACCTGAGCCGAAAAG11900.14669353993700807No Hit
ATATTGTACCTTGTCATTTGCAAATAGTGACAGTTTTACTTTTTCCTACC11890.14657026805470807No Hit
ACAGTGGACTGTCCCTCGTGGAGCTTGGATGGACTGAGCATTCCTGAGCC11810.145584092996308No Hit
TGGTATTACTGTCCAACTGAAAGGAAACATACTCTTAGAAGTGTATTTTG11640.1434884709972079No Hit
CTATACAATCTGTACCCCGATGATTAAATGCAATTCTAGTTCTCGAACTC11310.13942049888130767No Hit
ATATTAATATGGCTACTCAAAGCAACTCACTAGTCAGCTAGACATTAGAT11210.13818778005830762No Hit
CTATATTACCTGGCAAACAATATTTCGTGTTTCTAACTTATCTTTTCCTT10560.13017510770880716No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT9790.12068317277170663No Hit
GTCAATGACAGTGGACTGTCCCTCGTGGAGCTTGGATGGACTGAGCATTC9200.11341013171600622No Hit
CTTCAGGACCCCCCGGTTGGGGGGAACGTGCCCAGTAAAGAACTGAAATG9160.11291704418680622No Hit
GTGTGAGGCGTGACTGATTTGCAATTCAAAAAGCTCCAACAAAGGCAGAA8760.10798616889480593No Hit
TTCCTGAGCCCAGCAGGTGTCCTGGCTCTATTCTCTACGCATTGGGAAAT8200.10108294348600555No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)