FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_D10a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_D10a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493022
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTAATAGTTACCCTAGCTAGAAACCTCAAAACATTTTTAGTTCCTTC52911.0731772618666104No Hit
CTATTAGAACTGGGGTTTTATTGACAGAAACTGGTAAATTAGAAGGGGCA43210.8764314777028206No Hit
ACCTAATATAGTAGCATTTATAGGCAATGGTGCTAATAGTGAGAGTTTGT33360.6766432329591783No Hit
ATATTAGGTAAGTACTTAACACAGTACAATCTAGGCTGGCTCATCGACTT31020.6291808479134805No Hit
TCCTAAGAGTAATCTTGGAGACTTACACAGAGGGCATTAGAGCCTTATCA25370.514581499405706No Hit
TACTAGGACTTGGCAACTGGACCTAATAGCTAAGAGAAAGTGAGAAGGAA25260.5123503616471476No Hit
ATTCTAAGGTTACATGTTGTAAGATGTATTGCAGTGGAAAAGAAGTTGAG23450.47563800398359507No Hit
ATTTTATTTAGTGTCATGTTTTCAAGATTCATGCATGTTGTAGCATGTAT19880.4032274421831074No Hit
GTGTTAAGTACTTACCTAATATAGTAGCATTTATAGGCAATGGTGCTAAT19650.39856233596066704No Hit
CTATTAGCACCATTGCCTATAAATGCTACTATATTAGGTAAGTACTTAAC19410.39369439903290326No Hit
ATATAGTAGCATTTATAGGCAATGGTGCTAATAGTGAGAGTTTGTGAAAG19010.3855811708199634No Hit
ATGTATTATTTGTCCTTTTGTGTCTGCTTTATTTTATTTAGTGTCATGTT17540.3557650571374097No Hit
AGATTGTACTGTGTTAAGTACTTACCTAATATAGTAGCATTTATAGGCAA17200.3488688131564109No Hit
GTATTAAAGTACATGTAACAGAATTTGCCAGTGTAATCATCTTTAAGTGT17200.3488688131564109No Hit
CTTCAAACTAATCCTTCCAAAATCCAAAATTATTTCTCCTTTCAAAATTC16740.33953860071153014No Hit
ACTTAACACAGTACAATCTAGGCTGGCTCATCGACTTAATAACTCAACTT16660.3379159550689422No Hit
CCCTTAGGCATGCCAGCCACTCTCATGGCCAAAAATACTATCACTGTCTG16600.3366989708370012No Hit
GTTAAGTACTTACCTAATATAGTAGCATTTATAGGCAATGGTGCTAATAG16310.33081688038261986No Hit
GTGCTAATAGTGAGAGTTTGTGAAAGAGCAGTTACTAGGACTTGGCAACT16180.3281800812134144No Hit
GTTGTGGACCATCGCCATCATCCATCTGCAGAACTTTCTTGCTCTTCCAA16160.3277744198027674No Hit
GTACAGTACTGAGTCCTTTGAGTCCCACTGGCAAATCCTAAGAGTAATCT15200.3083026720917119No Hit
ATGTATTGCAGTGGAAAAGAAGTTGAGTTATTAAGTCGATGAGCCAGCCT13860.28112335757836365No Hit
GCCTAAGGGAGTGGGAAGTACAAAGAGCAGGTTCTGGGGCAGATCAAAAT13550.2748356057133353No Hit
CTTACACAGAGGGCATTAGAGCCTTATCATCACTTCAAACTAATCCTTCC13540.2746327750080118No Hit
GTTCTAATAGTTCTGATACATGCTACAACATGCATGAATCTTGAAAACAT13250.2687506845536305No Hit
GTGCAGCAGCTACTGAAAACAATATGGCAGTTCATCAAAAAATTAAAAAT12780.2592176414034262No Hit
GTTATGAGTACAGTACTGAGTCCTTTGAGTCCCACTGGCAAATCCTAAGA12780.2592176414034262No Hit
CTGCTGCACCATGCCGCATTCCCAACAGCAGAATTTTAAGTGTTCTAGTT12580.25516102729695633No Hit
TATGAGTACAGTACTGAGTCCTTTGAGTCCCACTGGCAAATCCTAAGAGT12390.25130724389580994No Hit
GTCCTAGTAACTGCTCTTTCACAAACTCTCACTATTAGCACCATTGCCTA11950.24238269286157615No Hit
GACTTACACAGAGGGCATTAGAGCCTTATCATCACTTCAAACTAATCCTT11950.24238269286157615No Hit
GCCCTAGGAAGAGTAAGAAAGAAAGGGGGGTGCAAGGTGTTTGGCCTGAG11680.2369062638178418No Hit
CAATATGGCAGTTCATCAAAAAATTAAAAATTAAAAACGGATTACCGTAT11090.22493925220375563No Hit
CTACTATATTAGGTAAGTACTTAACACAGTACAATCTAGGCTGGCTCATC11010.22331660656116767No Hit
GCTGTACACATTACCTAACAAGTCAGCAAAGATGAGGTCTAAAATGTGAA10830.21966565386534473No Hit
GTTCTGATACATGCTACAACATGCATGAATCTTGAAAACATGACACTAAA10830.21966565386534473No Hit
CATTAGAGCCTTATCATCACTTCAAACTAATCCTTCCAAAATCCAAAATT10750.21804300822275677No Hit
GTACTTAACACAGTACAATCTAGGCTGGCTCATCGACTTAATAACTCAAC10650.21601470116952184No Hit
GTTGTAAGATGTATTGCAGTGGAAAAGAAGTTGAGTTATTAAGTCGATGA10550.21398639411628692No Hit
GGGCATTAGAGCCTTATCATCACTTCAAACTAATCCTTCCAAAATCCAAA10440.21175525635772846No Hit
GTACTGAGTCCTTTGAGTCCCACTGGCAAATCCTAAGAGTAATCTTGGAG10390.210741102831111No Hit
TAGTAGATCTGATAGTATCAAAGTAGTATTCTAAGGTTACATGTTGTAAG10090.20465618167140615No Hit
TTTATAGGCAATGGTGCTAATAGTGAGAGTTTGTGAAAGAGCAGTTACTA10060.20404768955543567No Hit
GTACTGTGTTAAGTACTTACCTAATATAGTAGCATTTATAGGCAATGGTG10000.20283070532349468No Hit
GTAGTATTCTAAGGTTACATGTTGTAAGATGTATTGCAGTGGAAAAGAAG9890.20059956756493624No Hit
GTTCATGGCATTGTTGTGGACCATCGCCATCATCCATCTGCAGAACTTTC9860.19999107544896574No Hit
ATCCTGATGAGTGTGTAGTTTGCTCTACTGAGGCTTTGATTCACATTTTA9820.1991797526276718No Hit
GTCCACAACAATGCCATGAACTGTACACTTAAAGATGATTACACTGGCAA9770.19816559910105433No Hit
GTATCAAAGTAGTATTCTAAGGTTACATGTTGTAAGATGTATTGCAGTGG9740.19755710698508383No Hit
ATCCAGGCATCCCTGGATTTTGTTTTTAGAATATGGGAAGATCTTGACTA9660.1959344613424959No Hit
GACTATTACTTAACCTTAAAAAGGAATGAAGTTCTAATAGTTCTGATACA9480.19228350864667298No Hit
AAATAAAATAAAGCAGACACAAAAGGACAAATAATACATAATTCCACTTA9440.191472185825379No Hit
GTATAGAGTTTGGAAGAGCAAGAAAGTTCTGCAGATGGATGATGGCGATG9320.18903821736149706No Hit
CAATGGACTATTACTTAACCTTAAAAAGGAATGAAGTTCTAATAGTTCTG9240.18741557171890907No Hit
GATGTGAGATGACCAAGATGTAGACCAGAATGAAGATGAAGCAGTGAGAA9230.1872127410135856No Hit
GTACAATCTAGGCTGGCTCATCGACTTAATAACTCAACTTCTTTTCCACT9170.18599575678164465No Hit
ATCTGATAGTATCAAAGTAGTATTCTAAGGTTACATGTTGTAAGATGTAT8940.18133065055920425No Hit
AGCTAAGAGAAAGTGAGAAGGAACTAAAAATGTTTTGAGGTTTCTAGCTA8930.18112781985388074No Hit
GCCATATTGTTTTCAGTAGCTGCTGCACCATGCCGCATTCCCAACAGCAG8890.1803164970325868No Hit
AAACTAATCCTTCCAAAATCCAAAATTATTTCTCCTTTCAAAATTCACTG8700.17646271363144037No Hit
AAGTAGTATTCTAAGGTTACATGTTGTAAGATGTATTGCAGTGGAAAAGA8550.17342025305158798No Hit
GTGTACAGCTGATCAGTGTCCTCCTCCTTTTACTCTACCCGTCTGGTTTT8540.17321742234626447No Hit
AGATGGGACCCTGCAAAAGAAGACGATGAAGAGATCTCTGGGAAATGAAT8450.17139194599835303No Hit
GGAGTATAGTGTGTGTCTACTTCCAAAGTCAAGTCTGAAACTAAAAATCA8430.170986284587706No Hit
ATTTAGTGTCATGTTTTCAAGATTCATGCATGTTGTAGCATGTATCAGAA8370.16976930035576507No Hit
GGTTACATGTTGTAAGATGTATTGCAGTGGAAAAGAAGTTGAGTTATTAA8260.1675381625972066No Hit
TCCCTGGATTTTGTTTTTAGAATATGGGAAGATCTTGACTATTTCAGCAT8120.16469853272267768No Hit
CTCATCAGGATGGCTACTAACAAAAACAAACAAACAAACAAAATAACAGT8050.16327871778541322No Hit
ATAATACATAATTCCACTTACAAGAGATGCATAAAGAAACTGGACTGGAT8020.16267022566944275No Hit
GTAATAGTCCATTGTGTGTCTTTATCAACATTTTGTTCACGTGTTCACCT7980.16185890284814877No Hit
CTTGTAAGTGGAATTATGTATTATTTGTCCTTTTGTGTCTGCTTTATTTT7950.16125041073217827No Hit
GCTTTATTTTATTTAGTGTCATGTTTTCAAGATTCATGCATGTTGTAGCA7850.15922210367894332No Hit
ACTCTATACACATTAAACACATCTTGCCAGCCCTCCTTCCCCTCAGTCCC7830.15881644226829636No Hit
AGCCTAGATTGTACTGTGTTAAGTACTTACCTAATATAGTAGCATTTATA7780.15780228874167887No Hit
CATCAGGATGGCTACTAACAAAAACAAACAAACAAACAAAATAACAGTCC7750.1571937966257084No Hit
TAGTGAGAGTTTGTGAAAGAGCAGTTACTAGGACTTGGCAACTGGACCTA7750.1571937966257084No Hit
TATCAGAACTATTAGAACTTCATTCCTTTTTAAGGTTAAGTAATAGTCCA7660.15536832027779693No Hit
GTCTTTTATCTTGAAAACCAAAGAATCTGCTTTGTCCTGTCCTAGTATCC7560.153340013224562No Hit
CCTGATGAGTGTGTAGTTTGCTCTACTGAGGCTTTGATTCACATTTTAGA7500.152123028992621No Hit
GTACTGAAGGTACAGCCCTAGGAAGAGTAAGAAAGAAAGGGGGGTGCAAG7370.14948622982341558No Hit
CATTAAACACATCTTGCCAGCCCTCCTTCCCCTCAGTCCCTGGCAATCCA7220.14644376924356317No Hit
TACTATCACTGTCTGAGTTGCTTTCAACAAAACTGAACTGTGCTTCGCTC7200.14603810783291618No Hit
CTATTAGAACTTCATTCCTTTTTAAGGTTAAGTAATAGTCCATTGTGTGT7170.1454296157169457No Hit
TAGTTACCCTAGCTAGAAACCTCAAAACATTTTTAGTTCCTTCTCACTTT7050.14299564725306374No Hit
GTACTTACCTAATATAGTAGCATTTATAGGCAATGGTGCTAATAGTGAGA6990.1417786630211228No Hit
GAGTAATCTTGGAGACTTACACAGAGGGCATTAGAGCCTTATCATCACTT6950.14096734019982882No Hit
GCATTAGAGCCTTATCATCACTTCAAACTAATCCTTCCAAAATCCAAAAT6920.14035884808385832No Hit
ACACAGTACAATCTAGGCTGGCTCATCGACTTAATAACTCAACTTCTTTT6870.13934469455724086No Hit
GTAGATCTGATAGTATCAAAGTAGTATTCTAAGGTTACATGTTGTAAGAT6750.13691072609335891No Hit
ATCTAGGCTGGCTCATCGACTTAATAACTCAACTTCTTTTCCACTGCAAT6710.13609940327206493No Hit
GGACTATTACTTAACCTTAAAAAGGAATGAAGTTCTAATAGTTCTGATAC6550.132854111986889No Hit
ATGTTGTAGCATGTATCAGAACTATTAGAACTTCATTCCTTTTTAAGGTT6540.13265128128156553No Hit
GCATAGTAGATCTGATAGTATCAAAGTAGTATTCTAAGGTTACATGTTGT6520.13224561987091854No Hit
CCACTGAGCTGCATCACTAACTAACGAAAGAAGACTGCAGGAGGAACAGG6480.13143429704962456No Hit
GTTATTTTGTTTGTTTGTTTGTTTTTGTTAGTAGCCATCCTGATGAGTGT6470.13123146634430105No Hit
GTATTATTTGTCCTTTTGTGTCTGCTTTATTTTATTTAGTGTCATGTTTT6430.13042014352300707No Hit
TTTCAGCATAGTAGATCTGATAGTATCAAAGTAGTATTCTAAGGTTACAT6370.12920315929106613No Hit
TTATTTCTCCTTTCAAAATTCACTGCCCCTTCTAATTTACCAGTTTCTGT6310.12798617505912516No Hit
ATTTGAGCCCCTGCTTTTAATTCCTTTGGAAATAACACCCAGAAGTGCAT6280.12737768294315466No Hit
ATCATACGGTAATCCGTTTTTAATTTTTAATTTTTTGATGAACTGCCATA6230.1263635294165372No Hit
GTCCTATGTGACTCCATTGGAAAAGGACTCTGGAGTCTCTGACTGGTTTC6210.1259578680058902No Hit
GCCATGAACTGTACACTTAAAGATGATTACACTGGCAAATTCTGTTACAT6120.12413239165797875No Hit
TCCTAGGGCTGTACCTTCAGTACAGAGTCAGATTAGGGTCCCTTCTCACT6070.12311823813136127No Hit
CTACAACATGCATGAATCTTGAAAACATGACACTAAATAAAATAAAGCAG6050.12271257672071428No Hit
CACAATGGACTATTACTTAACCTTAAAAAGGAATGAAGTTCTAATAGTTC6040.12250974601539079No Hit
TTGTAGCATGTATCAGAACTATTAGAACTTCATTCCTTTTTAAGGTTAAG6030.1223069153100673No Hit
GCCTTATCATCACTTCAAACTAATCCTTCCAAAATCCAAAATTATTTCTC6020.12210408460474381No Hit
TTACCTAACAAGTCAGCAAAGATGAGGTCTAAAATGTGAATCAAAGCCTC6020.12210408460474381No Hit
AACTAATCCTTCCAAAATCCAAAATTATTTCTCCTTTCAAAATTCACTGC6020.12210408460474381No Hit
GTGATAGTATTTTTGGCCATGAGAGTGGCTGGCATGCCTAAGGGAGTGGG5980.12129276178344983No Hit
CTATTACTTAACCTTAAAAAGGAATGAAGTTCTAATAGTTCTGATACATG5960.12088710037280283No Hit
ATTACTTAACCTTAAAAAGGAATGAAGTTCTAATAGTTCTGATACATGCT5940.12048143896215585No Hit
GATAGTATCAAAGTAGTATTCTAAGGTTACATGTTGTAAGATGTATTGCA5940.12048143896215585No Hit
GTCCTTTTGTGTCTGCTTTATTTTATTTAGTGTCATGTTTTCAAGATTCA5910.11987294684618537No Hit
GTGTTTGGCCTGAGATAATGAAACACCACTGAGCTGCATCACTAACTAAC5890.11946728543553836No Hit
GTGTTTAATGTGTATAGAGTTTGGAAGAGCAAGAAAGTTCTGCAGATGGA5860.1188587933195679No Hit
ACACAGAGGGCATTAGAGCCTTATCATCACTTCAAACTAATCCTTCCAAA5840.1184531319089209No Hit
GCTTTGTCCTGTCCTAGTATCCATTTCTCTGTTCTTCTGTAGAGTGCTCT5830.1182503012035974No Hit
CTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAA5790.11743897838230344No Hit
CTCTATACACATTAAACACATCTTGCCAGCCCTCCTTCCCCTCAGTCCCT5700.11561350203439197No Hit
GTAAGATGTATTGCAGTGGAAAAGAAGTTGAGTTATTAAGTCGATGAGCC5680.11520784062374499No Hit
TTCTAATAGTTACCCTAGCTAGAAACCTCAAAACATTTTTAGTTCCTTCT5630.11419368709712752No Hit
CCGTATGATCCAGCAAATGCACTTCTGGGTGTTATTTCCAAAGGAATTAA5600.11358519498115703No Hit
TGCTACAACATGCATGAATCTTGAAAACATGACACTAAATAAAATAAAGC5590.11338236427583352No Hit
TCTGTAGAGTGCTCTTCCCCTTTCCTAAGCACATTCCAGTTGGGCTTTGA5550.11257104145453954No Hit
ACATAATTCCACTTACAAGAGATGCATAAAGAAACTGGACTGGATTGCCA5520.11196254933856908No Hit
ATCTTGGAGACTTACACAGAGGGCATTAGAGCCTTATCATCACTTCAAAC5490.11135405722259858No Hit
GTATTGCAGTGGAAAAGAAGTTGAGTTATTAAGTCGATGAGCCAGCCTAG5460.1107455651066281No Hit
TTATTTGTCCTTTTGTGTCTGCTTTATTTTATTTAGTGTCATGTTTTCAA5460.1107455651066281No Hit
GGTAAGTACTTAACACAGTACAATCTAGGCTGGCTCATCGACTTAATAAC5460.1107455651066281No Hit
GTTGTAGCATGTATCAGAACTATTAGAACTTCATTCCTTTTTAAGGTTAA5460.1107455651066281No Hit
ATGCTACAACATGCATGAATCTTGAAAACATGACACTAAATAAAATAAAG5440.11033990369598112No Hit
AATAGTGAGAGTTTGTGAAAGAGCAGTTACTAGGACTTGGCAACTGGACC5400.10952858087468714No Hit
TATCATCACTTCAAACTAATCCTTCCAAAATCCAAAATTATTTCTCCTTT5390.10932575016936363No Hit
GGTCCTATGTGACTCCATTGGAAAAGGACTCTGGAGTCTCTGACTGGTTT5370.10892008875871666No Hit
GGTCTGAGCGAAGCACAGTTCAGTTTTGTTGAAAGCAACTCAGACAGTGA5340.10831159664274617No Hit
CTACAGAAGAACAGAGAAATGGATACTAGGACAGGACAAAGCAGATTCTT5330.10810876593742268No Hit
GCAATACATCTTACAACATGTAACCTTAGAATACTACTTTGATACTATCA5280.10709461241080519No Hit
CCTTAGAATACTACTTTGATACTATCAGATCTACTATGCTGAAATAGTCA5240.1062832895895112No Hit
AAATAAAGCAGACACAAAAGGACAAATAATACATAATTCCACTTACAAGA5230.10608045888418773No Hit
ATCTTTAAGTGTACAGTTCATGGCATTGTTGTGGACCATCGCCATCATCC5210.10567479747354075No Hit
CTTCTGTAGAGTGCTCTTCCCCTTTCCTAAGCACATTCCAGTTGGGCTTT5180.10506630535757025No Hit
GATCTTGACTATTTCAGCATAGTAGATCTGATAGTATCAAAGTAGTATTC5130.10405215183095279No Hit
CCTTTTACTCTACCCGTCTGGTTTTCTTGTGGACACTGACCCTTTTTGTC5090.1032408290096588No Hit
GTTACATGTTGTAAGATGTATTGCAGTGGAAAAGAAGTTGAGTTATTAAG5080.1030379983043353No Hit
GCCAGGGACTGAGGGGAAGGAGGGCTGGCAAGATGTGTTTAATGTGTATA5080.1030379983043353No Hit
TTTTAGACCTCATCTTTGCTGACTTGTTAGGTAATGTGTACAGCTGATCA5060.10263233689368832No Hit
GAGTAAAAGGAGGAGGACACTGATCAGCTGTACACATTACCTAACAAGTC5030.10202384477771784No Hit
TGCCAGGACTGTTATTTTGTTTGTTTGTTTGTTTTTGTTAGTAGCCATCC5030.10202384477771784No Hit
CTTATCATCACTTCAAACTAATCCTTCCAAAATCCAAAATTATTTCTCCT5000.10141535266174734No Hit
TATCAAAGTAGTATTCTAAGGTTACATGTTGTAAGATGTATTGCAGTGGA5000.10141535266174734No Hit
TCCCAGCACCCTGGGATCATGACCTGAGCTGAAGGCGGACTTCACTGACT4980.10100969125110036No Hit
GTTCTGGGGCAGATCAAAATTGAGAGGCCTGGGGCATGACAAAAAGGGTC4940.10019836842980638No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)