FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_C9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_C9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5378166
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGATCTCTTAAGATGGAAGTGCCAGCAGCTACAGATCCTTCCAAGG265880.49436927011921905No Hit
ATACAGAGGAGACGACTTTTACATTTGTTTTTCTTTTCATTCCTGTTGAA217300.40404108017491464No Hit
GTGGTAAGGGGAGTGAAGGGAAGAGAGAAATTCAGTTTCCTGGAAGATTT206090.3831975435492322No Hit
CCTCTGTATAGATCTCTTAAGATGGAAGTGCCAGCAGCTACAGATCCTTC200690.3731569460667447No Hit
GATCTATACAGAGGAGACGACTTTTACATTTGTTTTTCTTTTCATTCCTG190540.35428434153947647No Hit
GTCTACACACAGAGCACCTGTGACCCATGTTCCTCCCTGCCCCATCCGCA186650.34705139261227713No Hit
GTACTACAGCCTGGGCAACGGAGCGAGACTCTGTCTCAAAAAAACACAAA144210.2681397338795418No Hit
CTGTACTACAGCCTGGGCAACGGAGCGAGACTCTGTCTCAAAAAAACACA137790.25620257909480665No Hit
GTCTTAACATGCGTGCTGTGAGTCCTCCGCAAAGTAGGGACAGTAATACG133860.24889525537144078No Hit
GTCCGAAGACCTACCAGAGAACATGCAGTTCAACAGGAATGAAAAGAAAA126860.23587966604229024No Hit
GGTAAGGGGAGTGAAGGGAAGAGAGAAATTCAGTTTCCTGGAAGATTTGT119640.2224550153342236No Hit
TGTTAAGACACTTTCTTTTGGTCATAGCTGGAGCCCATATTTGAAGCGGA119580.22234345313997375No Hit
ATCCAAGCTATCCCCTCAGTGAAACAAATGCTTGAGAAAGAAGATTTTTA111230.2068177144402014No Hit
GTGCCAGGCAGTGCTCTAAGTGGATGTCACTGAAGTCCTGTAGCCAGGAG108370.20149991651429133No Hit
GCTCTAAGTGGATGTCACTGAAGTCCTGTAGCCAGGAGCTATTCGTATTA106570.19815305068679548No Hit
GAACATAGATTTAAATTCAGAAGCTGGGAGTATATCCTGAGGACAGACAC106490.19800430109446232No Hit
TGTCTGGACTGAGGAAAATCCCTCTGGTACCCACTGTCCTTTCCTCTATG106050.19718617833663No Hit
GCCTTTTATGGGAGGGATTTTTTTTAAAAACAAACTCCACCAGCTCAGGT105330.19584743200563165No Hit
GTCCAGACACCTGAGTCCAGTCCCTGGGGACTTCAGGCTGGGTTATTTTG104760.19478759116025798No Hit
TCGTATTACTGTCCCTACTTTGCGGAGGACTCACAGCACGCATGTTAAGA102870.19127338204138733No Hit
GTAATACGAATAGCTCCTGGCTACAGGACTTCAGTGACATCCACTTAGAG101450.18863307677747396No Hit
CCCTAAACCAGCTCATCCGCTTCAAATATGGGCTCCAGCTATGACCAAAA99530.18506308656147838No Hit
GGTTTAGGGGCCGTACTCAGCTGGTTGAGTGTCATAGAGGAAAGGACAGT97070.18048903659723406No Hit
TCCCTGCCCCATCCGCAGGGGTGCCTGCTCCTCCAAGATTAGACTAAGGA96610.17963372644131848No Hit
GCCTGGCACCTTGGAAGGATCTGTAGCTGCTGGCACTTCCATCTTAAGAG95380.17734670145919632No Hit
ACTTAGAGCACTGCCTGGCACCTTGGAAGGATCTGTAGCTGCTGGCACTT94120.17500389537994923No Hit
GAATATGGGAGGCGAAGGTTGCAGTGAGCCAAGATCGCACCACTGTACTA90310.16791969604508303No Hit
GGATAGTGGTAAGGGGAGTGAAGGGAAGAGAGAAATTCAGTTTCCTGGAA90000.16734329137479206No Hit
GAGTAGATGAAGTCTACACACAGAGCACCTGTGACCCATGTTCCTCCCTG89780.1669342299958759No Hit
GAGTATATCCTGAGGACAGACACAAAGAAACCCCATGGTCCGAAGACCTA89460.16633923162654332No Hit
GTCCTTTCCTCTATGACACTCAACCAGCTGAGTACGGCCCCTAAACCAGC89240.16593017024762716No Hit
CCTGTGACCCATGTTCCTCCCTGCCCCATCCGCAGGGGTGCCTGCTCCTC84260.15667050812488867No Hit
GGTACCCACTGTCCTTTCCTCTATGACACTCAACCAGCTGAGTACGGCCC79980.14871240493506524No Hit
GTCTCAACCTGAGCTGGTGGAGTTTGTTTTTAAAAAAAATCCCTCCCATA79570.14795006327435783No Hit
GCTGAGTACGGCCCCTAAACCAGCTCATCCGCTTCAAATATGGGCTCCAG77440.14398960537848776No Hit
GCTCTGTGATGAGCCCTGAGATGTTTGAAGTGAGAACTCTCCAAACAGCC77220.1435805439995716No Hit
GGTTGAGACAGGCAAATTTCTTTCTTATCTTTCTATGCCTAAAAATCTTC75100.13963867980274317No Hit
GTATTACTGTCCCTACTTTGCGGAGGACTCACAGCACGCATGTTAAGACA74900.1392668058219103No Hit
AGCTTGGATAGCCTCATTTTAGTTGATGTCACCATTTCAATCCCTACTTT74230.13802102798612018No Hit
ATAAAAGGCTGGGCAGAGGGGCGTTACACCTGAGGGAATTTTCCAGATTA73780.1371843115292462No Hit
CCCTAGCTCCCTCATGCTCCACGACTTGGGGACAAGCTAGTTGTCTTCTC73620.13688681234457992No Hit
CAGTAATACGAATAGCTCCTGGCTACAGGACTTCAGTGACATCCACTTAG71900.13368869610941722No Hit
GCCCTGAGATGTTTGAAGTGAGAACTCTCCAAACAGCCTCCGTGGGTATA71810.13352135281804242No Hit
ACTACAGCCTGGGCAACGGAGCGAGACTCTGTCTCAAAAAAACACAAACA71100.13220120018608575No Hit
GAGCACCTGTGACCCATGTTCCTCCCTGCCCCATCCGCAGGGGTGCCTGC70710.13147604592346163No Hit
ATTCAGGAGACTGCATGGAACATAGATTTAAATTCAGAAGCTGGGAGTAT70450.13099260974837892No Hit
AGATTAGACTAAGGACAAAATAACCCAGCCTGAAGTCCCCAGGGACTGGA69860.12989558150492195No Hit
AAATAACCCAGCCTGAAGTCCCCAGGGACTGGACTCAGGTGTCTGGACTG69800.12978401931067207No Hit
GTTCAACAGGAATGAAAAGAAAAACAAATGTAAAAGTCGTCTCCTCTGTA69060.12840808558159048No Hit
GCATGGAACATAGATTTAAATTCAGAAGCTGGGAGTATATCCTGAGGACA68700.1277387124160913No Hit
GGAAAGGACAGTGGGTACCAGAGGGATTTTCCTCAGTCCAGACACCTGAG68080.12658590307550938No Hit
GTCTTACTCTGTCACCCAGGCTGGAGTGCAGTGGCATGATCTTGGCTCAC65840.12242091449018123No Hit
TCTCAGGGCTCATCACAGAGCTTTCCATGCATGTTCCCAGGCAAGTCTCT65070.12098919966397467No Hit
CTACAGATCCTTCCAAGGTGCCAGGCAGTGCTCTAAGTGGATGTCACTGA64380.11970623443010127No Hit
TCACAGAGCTTTCCATGCATGTTCCCAGGCAAGTCTCTTGTGGTCAGGGA64130.11924139195406017No Hit
CCTGAGGACAGACACAAAGAAACCCCATGGTCCGAAGACCTACCAGAGAA62810.11678702368056323No Hit
GTTCGTTGAGATGGCATTTCCATGGAATTCAGGAGACTGCATGGAACATA62700.11658249299110514No Hit
GTAGATGAAGTCTACACACAGAGCACCTGTGACCCATGTTCCTCCCTGCC62330.11589452612656434No Hit
GTGGGTACCAGAGGGATTTTCCTCAGTCCAGACACCTGAGTCCAGTCCCT62180.11561562064093966No Hit
CTGTATAGATCTCTTAAGATGGAAGTGCCAGCAGCTACAGATCCTTCCAA61750.114816091582149No Hit
AGTCTACACACAGAGCACCTGTGACCCATGTTCCTCCCTGCCCCATCCGC61160.11371906333869203No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT60040.11163656904602796No Hit
CTTCCAAGGTGCCAGGCAGTGCTCTAAGTGGATGTCACTGAAGTCCTGTA58680.10910782597636443No Hit
CTTAAGATGGAAGTGCCAGCAGCTACAGATCCTTCCAAGGTGCCAGGCAG58340.10847564020894855No Hit
TCTATACAGAGGAGACGACTTTTACATTTGTTTTTCTTTTCATTCCTGTT58280.10836407801469868No Hit
GTCTTCGGACCATGGGGTTTCTTTGTGTCTGTCCTCAGGATATACTCCCA58090.10801079773290748No Hit
GTCCCTACTTTGCGGAGGACTCACAGCACGCATGTTAAGACACTTTCTTT55040.10233971952520618No Hit
CCTCTATGACACTCAACCAGCTGAGTACGGCCCCTAAACCAGCTCATCCG54500.10133565977695742No Hit
GTACTCAGCTGGTTGAGTGTCATAGAGGAAAGGACAGTGGGTACCAGAGG54500.10133565977695742No Hit
CCTTGGAAGGATCTGTAGCTGCTGGCACTTCCATCTTAAGAGATCTATAC54470.10127987867983249No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)