FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_C8a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_C8a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences973720
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGAACTGAGAGCCACTTCTATCTAGAAGTCATTTTCTGTAACGAAC46290.4753933368935629No Hit
GCCCTAAGAAGCCATGTGAAATTTAAGAGTAACCATTTAACTACAAGATA37020.38019143080146245No Hit
CCATTTAACTACAAGATATGCAAAAACCTTCTGAAATACTCGGGAGTATG34390.35318161278396254No Hit
ATTTAAGAGTAACCATTTAACTACAAGATATGCAAAAACCTTCTGAAATA34130.3505114406605595No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA29420.30214024565583536No Hit
TTATAAAAGTGGCTATGTAGTATTGTTAGTTTTGTCTCTAGATAAAGTTT28740.29515671856385817No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT27590.28334634186419094No Hit
ACTCAATACTCAGTAAGTTCAGGGATGTTTGGAAAGCCGGCCCTGCCTTT27210.2794437826069096No Hit
CTTCTATCTAGAAGTCATTTTCTGTAACGAACTAAAATTCCTCAGCTTGC27040.27769790083391527No Hit
GATTAGGACATTCCCCTGCTCCAAACCCTCCAGGGGCTCCCATTACAGTT26900.2762601158443906No Hit
GTAGTATTGTTAGTTTTGTCTCTAGATAAAGTTTATTGTCTCGCCTTTTA25330.2601363841761492No Hit
ATGTAGTATTGTTAGTTTTGTCTCTAGATAAAGTTTATTGTCTCGCCTTT24760.25428254529022715No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA24060.24709362034260363No Hit
GTGTCAGGACTTGGCAACCTCTTCTGTAAAGGACCGGATAGTAAATACTT23950.2459639321365485No Hit
GTTATAAGGTGGAGAAGTGGGGAACGCAGTGAACCAGAGAGGGAAGGCAC23010.2363102329211683No Hit
GTCCTGACACAGGGCACTGAGTTTTATTCTAACTGTAATGGGAGCCCCTG21770.22357556587109229No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT21750.2233701680154459No Hit
GTCCAAGTCTGGCCCACCACTTGTTCTTGTAAACCAAGTTTTATTGGAAC20890.21453806022265126No Hit
GTACGTTAGGAGTCTTTCCAAGTTCTTTTTTGTGTGTCCTTTAACTAGTT20760.21320297416094974No Hit
CCTTATAACTTTCCTCAATTTAGGGAGTGGAGTTGATCCTCATCTTTGCC19660.2019060921003985No Hit
CCCTTACAAATTGTCTGTGGCTGCTTTTGTGCTACAACAGCAGAACCAAA19200.19718194142053155No Hit
CTACAAGATATGCAAAAACCTTCTGAAATACTCGGGAGTATGCAAGCTGA18810.19317668323542703No Hit
ATCTAGAAGTCATTTTCTGTAACGAACTAAAATTCCTCAGCTTGCATACT18700.1920469950293719No Hit
GTTCAGGGATGTTTGGAAAGCCGGCCCTGCCTTTGACGAGTTTGTTCCCT18610.19112270467896317No Hit
CATTTGGGTGGTACGTTAGGAGTCTTTCCAAGTTCTTTTTTGTGTGTCCT17630.1810582097522902No Hit
CCTTTAACTAGTTGATGCCTAGTGGAAATCGGCCTTGTTACCAATTTAGA17370.17838803762888716No Hit
CGTTAGGAGTCTTTCCAAGTTCTTTTTTGTGTGTCCTTTAACTAGTTGAT16430.16873433841350696No Hit
GTATTGAGTTGGGGGCAGAGCCCAGTGGGAGAGGCGTCAAGTGCATCCCA16080.1651398759396952No Hit
CTCCATGTTTGAGGAGTGAGTGAGTGAATAAACTAGCCATGTTCCATGAA16070.16503717701187198No Hit
CTAAGAAGCCATGTGAAATTTAAGAGTAACCATTTAACTACAAGATATGC16060.1649344780840488No Hit
ACTCGGGAGTATGCAAGCTGAGGAATTTTAGTTCGTTACAGAAAATGACT15740.1616481123937066No Hit
CCCTAAATTGAGGAAAGTTATAAGGTGGAGAAGTGGGGAACGCAGTGAAC15620.1604157252598283No Hit
GTTCTAAATTGGTAACAAGGCCGATTTCCACTAGGCATCAACTAGTTAAA15560.15979953169288913No Hit
TCCCTGAACTTACTGAGTATTGAGTTGGGGGCAGAGCCCAGTGGGAGAGG15370.15784825206424843No Hit
CCCCAGTGCCTGGGAAGTGGTTGCACGTGCTCTCCATGTTTGAGGAGTGA14300.14685946678716674No Hit
GCTCCAAACCCTCCAGGGGCTCCCATTACAGTTAGAATAAAACTCAGTGC14280.14665406893152036No Hit
TTTGAGGAGTGAGTGAGTGAATAAACTAGCCATGTTCCATGAATTTCTCA14040.1441892946637637No Hit
GATTGAAAGCGACAGAGAATAGTTTCGAACAAATAACATTTATGCAAGCC14010.14388119788029413No Hit
GGAGTATGCAAGCTGAGGAATTTTAGTTCGTTACAGAAAATGACTTCTAG13480.13843815470566487No Hit
AGATAGAAGTGGCTCTCAGTTCTAAATTGGTAACAAGGCCGATTTCCACT12770.13114653083021813No Hit
TCCTTTAACTAGTTGATGCCTAGTGGAAATCGGCCTTGTTACCAATTTAG12710.13053033726327898No Hit
CTATAAAAGGCGAGACAATAAACTTTATCTAGAGACAAAACTAACAATAC12700.1304276383354558No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG12480.12816826192334552No Hit
CTCCAGGGGCTCCCATTACAGTTAGAATAAAACTCAGTGCCCTGTGTCAG12470.12806556299552233No Hit
CCATATGGCCCACTAAGTTGAAAGTATTTACTATCCGGTCCTTTACAGAA12330.12662777800599762No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTC12140.12467649837735693No Hit
GGGCAGAAGTGTGCAGGAGGCAGCGGATCTGGCGGGACAGACCTGAGGAT12110.12436840159388736No Hit
GAACCAGAGACTGCAGGATTATCTGTGGCTTTCAATGATCTGTGTTCCGA12030.12354681017130181No Hit
TTAGGAGTCTTTCCAAGTTCTTTTTTGTGTGTCCTTTAACTAGTTGATGC12030.12354681017130181No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC12010.12334141231565543No Hit
CCCCAAAGCCCTAAGAAGCCATGTGAAATTTAAGAGTAACCATTTAACTA11970.12293061660436265No Hit
CTGCAGGATTATCTGTGGCTTTCAATGATCTGTGTTCCGAGGGTCTGATG11890.1221090251817771No Hit
GTGGAGAAGTGGGGAACGCAGTGAACCAGAGAGGGAAGGCACCTCCGCCT11850.12169822947048434No Hit
GAAATAAGGTGGTTAATTGAAGAAATGCGGCACAGATTGAAAGCGACAGA11720.1203631434087828No Hit
GTATGCAAGCTGAGGAATTTTAGTTCGTTACAGAAAATGACTTCTAGATA11640.11954155198619727No Hit
GACCTGAGGATGACTCGGACGTCACCTCCCTCCTCTGAGCCGCAGTGGCC11560.11871996056361171No Hit
CCTGTAGATAAGGGGGCAGCAAACCACAGGCTGCAGTCCAAGTCTGGCCC11530.11841186378014214No Hit
GTATTTACTATCCGGTCCTTTACAGAAGAGGTTGCCAAGTCCTGACACAG11450.11759027235755659No Hit
ACCAGAGACTGCAGGATTATCTGTGGCTTTCAATGATCTGTGTTCCGAGG11350.11656328307932465No Hit
GCCATGCCCACTCCCTTACAAATTGTCTGTGGCTGCTTTTGTGCTACAAC11150.11450930452286077No Hit
ACTACAAGATATGCAAAAACCTTCTGAAATACTCGGGAGTATGCAAGCTG11130.11430390666721439No Hit
GTGTTCCGAGGGTCTGATGTCAGCCATGGAGGATACAGCTGCTGCACCTT10860.11153103561598818No Hit
GTATTGTTAGTTTTGTCTCTAGATAAAGTTTATTGTCTCGCCTTTTATAG10860.11153103561598818No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT10730.11019594955428665No Hit
CTCCCGAGTATTTCAGAAGGTTTTTGCATATCTTGTAGTTAAATGGTTAC10730.11019594955428665No Hit
CCTCAAGGATGTGGGATGCACTTGACGCCTCTCCCACTGGGCTCTGCCCC10700.10988785277081707No Hit
GTCCTAATCTAATTTATATTTTTAAAATACCCCCCTGGTGGAGGAAAGAC10630.10916896027605473No Hit
CCTCTGAGCCGCAGTGGCCTCATTTCTAAGGCAAAGATGAGGATCAACTC10600.10886086349258513No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT10570.10855276670911555No Hit
CTTCTGTAAAGGACCGGATAGTAAATACTTTCAACTTAGTGGGCCATATG10520.1080392720699996No Hit
GGATACAGCTGCTGCACCTTCTGCAGGCCCCAAAGCCCTAAGAAGCCATG10520.1080392720699996No Hit
GTAGATAAGGGGGCAGCAAACCACAGGCTGCAGTCCAAGTCTGGCCCACC10510.10793657314217639No Hit
GAATAGTTTCGAACAAATAACATTTATGCAAGCCTTACAACTACTGCTTG10510.10793657314217639No Hit
CTTCTCCACCTTATAACTTTCCTCAATTTAGGGAGTGGAGTTGATCCTCA10460.10742307850306043No Hit
TTACAGAAGAGGTTGCCAAGTCCTGACACAGGGCACTGAGTTTTATTCTA10350.1062933902970053No Hit
GCTATGTAGTATTGTTAGTTTTGTCTCTAGATAAAGTTTATTGTCTCGCC10350.1062933902970053No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC10340.1061906913691821No Hit
GTAAAGGACCGGATAGTAAATACTTTCAACTTAGTGGGCCATATGGTCTC10300.10577989565788934No Hit
CTTCTGCCCACTTCTCACCACCTCTCCCGCAGCACCGCATCTCCCTGCAG9860.10126114283366883No Hit
GGCTCACACAGAAACCTGTAGATAAGGGGGCAGCAAACCACAGGCTGCAG9790.10054225033890646No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)