FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_C7a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_C7a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300891
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA14230.4729287349904118No Hit
GGATACACCATGCAATCTATTCGAGAAATTTTTCCCTAGCAGTTCATATA10840.36026335118032776No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT8400.27917086253826134No Hit
GTTCATTGATGCTTAATGCATGTCCATTATTCTCTTGTCTGCTGTAATTG8390.27883851627333484No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG8250.2741856685643638No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG7640.25391254640384725No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCATGGTGTAT7580.25191846881428825No Hit
CATAAATACGAGGCTTTTCATACGTAAAGTAAACGTCTTTATCGACGATC7350.2442745047209787No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG7290.2422804271314197No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA6900.22931892279928612No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT6890.2289865765343596No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA6520.21668976473207904No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA6430.21369864834774052No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG6350.21103987822832854No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC6090.20239887534023948No Hit
ATAAAGAAGGGATCGTCGATAAAGACGTTTACTTTACGTATGAAAAGCCT6060.20140183654546No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC5880.19541960377678297No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG5700.18943737100810593No Hit
GCTTAATGCATGTCCATTATTCTCTTGTCTGCTGTAATTGGGTATCTCAT5460.1814610606498699No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG5430.1804640218550904No Hit
GTATTAATGTAAACGTTTCTGTACGCTTATATGCAAACAACGACAACGTG5400.17946698306031086No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA5270.17514648161626636No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG5170.17182301896700136No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG4950.1645114011386183No Hit
CTATAAAGAAGGGATCGTCGATAAAGACGTTTACTTTACGTATGAAAAGC4610.15321162813111724No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG4510.14988816548185224No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT4430.14722939536244023No Hit
GTATTTATGTGTGTAAATGAACGAGCGTACGTAGTTATCTCTGTTTTCTT4270.1419118551236162No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG4170.13858839247435117No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG4130.13725900741464517No Hit
TTGCAAGATTGCTCAGTTCATTGATGCTTAATGCATGTCCATTATTCTCT4120.13692666114971866No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT3980.13227381344074765No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG3970.13194146717582114No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC3920.13027973585118863No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC3840.12762096573177661No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG3730.1239651568175851No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGCTAATGTGCTAATTGGGCAA3730.1239651568175851No Hit
CCCATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTA3680.1223034254929526No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT3660.12163873296309959No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG3640.12097404043324658No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT3610.1199770016384671No Hit
GAGTAAGACCTGCTGTTGCATCTTCGCCATTGTTTGCTTGGCGCCAAACT3610.1199770016384671No Hit
CTTATATGCAAACAACGACAACGTGTATCTCTGCGTTGAATACTCTTGCG3580.11897996284368757No Hit
CATATAGACTGTTGAATACAGATTAAACTTCCAAATGTGTGCATATTGCA3570.11864761657876108No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA3570.11864761657876108No Hit
GTGGAGGCCATGGTGTCTAGGGCCCGGATTGATGCCAGGGTCGACTTCGA3560.11831527031383457No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT3520.11698588525412858No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGCAATCTATTCGAGA3500.11632119272427556No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC3470.11532415392949606No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC3470.11532415392949606No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA3470.11532415392949606No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG3410.11333007633993705No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG3380.11233303754515755No Hit
GGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAG3370.11200069128023105No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGCAATCTATTCG3320.11033895995559853No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA3310.11000661369067204No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT3300.10967426742574554No Hit
ATCCAGAATAGCATAACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGA3290.10934192116081903No Hit
CCATTATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCAC3290.10934192116081903No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCATGGTGT3260.10834488236603952No Hit
ATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGA3260.10834488236603952No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA3240.10768018983618652No Hit
TCTATACACAGGTTGGATAGAAGAGCCTTTGTGGTCTAGAAAGGTTAGAT3210.10668315104140702No Hit
GTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTACGGTCTTGGGA3210.10668315104140702No Hit
GCCATGGAACAGGAACAGGATACACCATGCAATCTATTCGAGAAATTTTT3150.10468907345184801No Hit
GTATTCAACAGTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGA3140.10435672718692152No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT3130.10402438092199501No Hit
GTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAGG3090.10269499586228899No Hit
GGAAAGGAAGAGAGGAAGAAAAACCTAAAGAAGGAGAGAAAGGAAGAAAG3090.10269499586228899No Hit
ATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCACTTCAT3070.102030303332436No Hit
CACCTACATCTATCATACAGAAGTCTATTTCCATACAACCGATACGTATT3060.10169795706750949No Hit
GCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCT3030.10070091827273No Hit
ATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGACA3020.1003685720078035No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)