FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_C10a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_C10a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1140185
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATAGACTGTTGAATACATTGCAGCAAACTTGTTAGTTTCGATTTTCG157411.380565434556673No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC155491.3637260619987106No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG144791.2698816420142347No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG129441.135254366615944No Hit
ATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA126251.1072764507514132No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA126141.1063116950319465No Hit
GTTGAATACATTGCAGCAAACTTGTTAGTTTCGATTTTCGGATCTTCCCC109240.9580901344957179No Hit
GTATTCAACAGTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGA108120.9482671671702398No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA65230.5721001416436806No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT61450.5389476269201928No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTCGATCTTGGGGGGCTATATGA57150.5012344487955902No Hit
ATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATGTATT56350.494218043563106No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT53180.4664155378293873No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG52800.4630827453439574No Hit
ATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTG51240.4494007551406131No Hit
GTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGAT46680.4094072453154532No Hit
CTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAATT45630.4001982134478177No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC45330.3975670614856361No Hit
GATTAATGATCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACTCCTTCC41610.36494077715458456No Hit
ATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATATGGGGAA41570.36458995689296037No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA41120.360643228949688No Hit
ACCTGGAACCTGGAACCTTCGATCTTGGGGGGCTATATGAAGCAATCGAG41120.360643228949688No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC40090.35160960721286455No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATGTA39450.3459964830268772No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT37910.3324899029543451No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC37760.33117432697325433No Hit
GCTTAATGCATCTTGGTTCAACTCCTTCCTCACACATGCACTGAAGTAGT37500.32889399527269697No Hit
CGCATATAGACTGTTGAATACATTGCAGCAAACTTGTTAGTTTCGATTTT35130.3081078947714625No Hit
GTTCAGGCACTTAGGGACAACCTGGAACCTGGAACCTTCGATCTTGGGGG34560.3031087060433175No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG33580.29451360963352435No Hit
ATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG31060.27241193315119916No Hit
GAAAGAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAA29880.26206273543328495No Hit
GAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAACCCCTCAAGTTGT29500.25872994294785495No Hit
GTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAG29450.2582914176208247No Hit
GTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAACCCCTCA27890.2446094274174805No Hit
TCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAA27610.24215368558611106No Hit
TCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACTCCTTCCTCACACATG27470.24092581467042629No Hit
GGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTG27360.23996105895095973No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT26900.2359266259422813No Hit
GGGCTATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTT26370.2312782574757605No Hit
ATCTTGGGGGGCTATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCC26320.23083973214873021No Hit
ACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA25570.2242618522432763No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGT25400.22277086613137342No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGA25230.22127988001947055No Hit
AAAGAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAAT24720.21680692168376184No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA24160.2118954380210229No Hit
TAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAA24120.21154461775939867No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC23540.20645772396584763No Hit
CAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA23110.2026864061533874No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA23080.2024232909571692No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA22510.19742410222902423No Hit
GCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATAT22350.1960208211825274No Hit
ACAATGCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAG21690.19023228686572793No Hit
AAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCA21660.18996917166950977No Hit
GATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTC21500.18856589062301293No Hit
GAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCA20890.18321588163324373No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCC20800.18242653604458925No Hit
ATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGC20380.17874292329753505No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTG20310.17812898783969267No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT20120.1764625915969777No Hit
GTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACT19870.17426996496182637No Hit
GGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGA19550.1714634028688327No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA19490.1709371724763964No Hit
CTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA19400.1701478268877419No Hit
GTTTTCGGCTGAATCTAGAAAATTGCTTCTCATTGTTCAGGCACTTAGGG19040.166990444533124No Hit
CTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAA19000.1666396242714998No Hit
CCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTGCCTGAACA18710.16409617737472426No Hit
GAAGTAGTTGTGGCAATGCTACTATTTGCTATCCATACTGTCCAAAAAAG18690.16392076724391216No Hit
ATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCC18550.16269289632822745No Hit
GTCATAGAAGGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACT18350.16093879502010638No Hit
GTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTAC18190.15953551397360954No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA18170.15936010384279745No Hit
ATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAATTGC17960.15751829746927035No Hit
CTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATATG17820.1562904265535856No Hit
ATTCAACAGTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAAT17780.1559396062919614No Hit
TCATTGTTCAGGCACTTAGGGACAACCTGGAACCTGGAACCTTCGATCTT17430.15286992900274957No Hit
GCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCA17300.1517297631524709No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT17200.15085271249841034No Hit
TTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCA16990.14901090612488324No Hit
TCCCCATATTCTTTCATTGCCTTTTCCGCAAGCTCGACGATCATTGGATT16220.1422576160886172No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC16150.14164368063077484No Hit
CTTGAGGGGTTTTCGGCTGAATCTAGAAAATTGCTTCTCATTGTTCAGGC16120.14138056543455665No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA15960.13997728438805984No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT15560.1364690817718177No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA15460.1355920311177572No Hit
CACATGCACTGAAGTAGTTGTGGCAATGCTACTATTTGCTATCCATACTG15400.1350658007253209No Hit
TGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAACCCCTCAA15200.13331169941719984No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT15080.1322592386323272No Hit
AAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATA15040.131908418370703No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT15010.13164530317448486No Hit
GTTCAACTCCTTCCTCACACATGCACTGAAGTAGTTGTGGCAATGCTACT14860.13032972719339406No Hit
GTTTTGCTTAATGCATCTTGGTTCAACTCCTTCCTCACACATGCACTGAA14850.130242022127988No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTT14630.12831251068905486No Hit
CTGTTGAATACATTGCAGCAAACTTGTTAGTTTCGATTTTCGGATCTTCC14470.12690922964255802No Hit
TTTCTAGATTCAGCCGAAAACCCCTCAAGTTGTGGAGACGCATATAGACT14370.12603217898849747No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT13700.12015593960629196No Hit
ATCTAGAAAATTGCTTCTCATTGTTCAGGCACTTAGGGACAACCTGGAAC13670.1198928244100738No Hit
GGATAAAGAACAGGCGTGACAGCTGCTTCCTAAATAATCAACGGTGGGTG13570.11901577375601329No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT13310.11673544205545591No Hit
ACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAG13210.11585839140139538No Hit
GTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGT13090.11480593061652276No Hit
TGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATC13060.11454281542030459No Hit
GTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAA13040.11436740528949249No Hit
ATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGAT12830.11252559891596539No Hit
GGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCAT12440.10910510136512934No Hit
GGTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGA12410.10884198616891118No Hit
TTAATGATCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACTCCTTCCTC12090.10603542407591751No Hit
GACGCATATAGACTGTTGAATACATTGCAGCAAACTTGTTAGTTTCGATT12090.10603542407591751No Hit
GTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCAAT12040.10559689874888724No Hit
CCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGG12040.10559689874888724No Hit
GTTTGCTGCAATGTATTCAACAGTCTATATGCGTCTCCACAACTTGAGGG11910.10445673289860855No Hit
CCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAAC11720.10279033665589357No Hit
GTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTG11660.10226410626345724No Hit
ACTTGAGGGGTTTTCGGCTGAATCTAGAAAATTGCTTCTCATTGTTCAGG11510.10094853028236647No Hit
AATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATGTAT11410.10007147962830593No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)