FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_B7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_B7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5450829
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCAAAG633611.1624103416195959No Hit
ACATCTAGATGATCAAATAGTCCTTACTTATAACTAAACCACCTGAATCA450270.8260578345055404No Hit
CCCTTATTTCTCTGTAATCATCATCAAGGGGCTAAGGTATCAGTTCTCAA322320.5913228978564545No Hit
TCTCAGAAGAGTATCAAGAACAATTCAGAACATACAATATGTGTGTGATA314480.5769397645752601No Hit
GGATATTACTCTACATCTAGATGATCAAATAGTCCTTACTTATAACTAAA311620.5716928562609467No Hit
GTCCTTACTTATAACTAAACCACCTGAATCACCTATGATTTGAGGTACTA273990.5026574856778666No Hit
CTTATAACTAAACCACCTGAATCACCTATGATTTGAGGTACTACGTGTTA255270.46831408580236145No Hit
ACTTATAACTAAACCACCTGAATCACCTATGATTTGAGGTACTACGTGTT248570.45602237751358554No Hit
ATCAAATAGTCCTTACTTATAACTAAACCACCTGAATCACCTATGATTTG233750.42883385261214396No Hit
GTATGATAAACTTGAGTTACACTGAATTTTACTCAGTTCCCACAGAAGTA229340.4207433401414721No Hit
TAACTAAACCACCTGAATCACCTATGATTTGAGGTACTACGTGTTAGGAA223240.40955238184870596No Hit
GGATATGATTGCATAATTAAGGAAGTTTGGGTTTTTTTAAGAGTACTTCT215000.39443541523683834No Hit
ATTCAGAACATACAATATGTGTGTGATAACCACCTACTGGATGAATATTT202820.3720901903178397No Hit
ATCAAGAACAATTCAGAACATACAATATGTGTGTGATAACCACCTACTGG177620.3258586904854289No Hit
CAAATAACACTGTATGATAAACTTGAGTTACACTGAATTTTACTCAGTTC173800.3188505821775No Hit
ATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCAAAGAGAG164290.3014036947407449No Hit
CAATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCAAAGAG155670.28558958646473775No Hit
ATATAGGATTTGCTAAATGACCCAGAATGAGAGGAGATAAATCATAATCA140920.25852948239616397No Hit
CTCTACATCTAGATGATCAAATAGTCCTTACTTATAACTAAACCACCTGA138360.25383294907985554No Hit
CCTATGATTTGAGGTACTACGTGTTAGGAAATAAAGGCTCATCTTGAGAA130580.23955989079826207No Hit
ACTCTACATCTAGATGATCAAATAGTCCTTACTTATAACTAAACCACCTG129920.23834906580265128No Hit
GTTCTCAAGATGAGCCTTTATTTCCTAACACGTAGTACCTCAAATCATAG123050.2257454783483393No Hit
ATTAAGGAAGTTTGGGTTTTTTTAAGAGTACTTCTGTGGGAACTGAGTAA113630.20846370341098575No Hit
AAATAACACTGTATGATAAACTTGAGTTACACTGAATTTTACTCAGTTCC106840.19600688262280838No Hit
TACTAATACAGACCAGGGAAAGAAGGAGGAGGGAGAGGGGATGAGAAGAG105310.19319997013298346No Hit
GATTATAAAAGGTCACCAAAAAGGACAAAAAACATTAAAAAGCAATGTCC103060.18907215764794677No Hit
GTTTTTAAGTGTACAGTTGAGTGATTTTTAATCAGTTTGAGTATGCAGCT99920.18331156600216225No Hit
ATATAATTCCAAACTTCAATCAGTAATTCCAATGTGAAGGATGACTCAGT99250.18208239517328464No Hit
CATCTAGATGATCAAATAGTCCTTACTTATAACTAAACCACCTGAATCAC98240.18022946601333484No Hit
CTGTATGATAAACTTGAGTTACACTGAATTTTACTCAGTTCCCACAGAAG98190.18013773684700068No Hit
AAATAGTCCTTACTTATAACTAAACCACCTGAATCACCTATGATTTGAGG94970.17423037853508153No Hit
TTACTCTACATCTAGATGATCAAATAGTCCTTACTTATAACTAAACCACC86260.15825115775967288No Hit
TTATTAACCAGGATATTACTCTACATCTAGATGATCAAATAGTCCTTACT84500.15502229110471086No Hit
ATATAAAACAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATACACAC82680.15168334945014786No Hit
TATATAACCAGAACTAAAAATCAGGTATCATTCTTGTCCCATTTACTAAA82650.15162831195034737No Hit
GTGTACAGTTGAGTGATTTTTAATCAGTTTGAGTATGCAGCTATCATCAC80990.14858290362805365No Hit
GTATCAAGAACAATTCAGAACATACAATATGTGTGTGATAACCACCTACT80520.14772064946451266No Hit
ACTTTATGCTTTAAGAAAGTTTTGGGAAGTTTCTGATAAAATATTGGTTC79710.14623463696989944No Hit
GTGTTAGGAAATAAAGGCTCATCTTGAGAACTGATACCTTAGCCCCTTGA78750.1444734369762838No Hit
TCCTAACACGTAGTACCTCAAATCATAGGTGATTCAGGTGGTTTAGTTAT78490.14399644531134623No Hit
GTACTACGTGTTAGGAAATAAAGGCTCATCTTGAGAACTGATACCTTAGC76800.140895999489252No Hit
GTATATAATTCCAAACTTCAATCAGTAATTCCAATGTGAAGGATGACTCA76460.1402722411581798No Hit
GTATTATATAGGATTTGCTAAATGACCCAGAATGAGAGGAGATAAATCAT73210.13430984534646015No Hit
TTCTAGGACATTGCTTTTTAATGTTTTTTGTCCTTTTTGGTGACCTTTTA73080.13407134951399136No Hit
GAACATACAATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGG71440.13106263285823128No Hit
GAAATAAGGGAGGACCTCTCTTTGCCTCCAAATATTCATCCAGTAGGTGG70410.12917301203174783No Hit
CCATTAACTGGATATGATTGCATAATTAAGGAAGTTTGGGTTTTTTTAAG69870.12818233703533902No Hit
ACACTGTATGATAAACTTGAGTTACACTGAATTTTACTCAGTTCCCACAG66700.12236670788975401No Hit
CATACAATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCAA66040.12115588289414325No Hit
CTGTAATCATCATCAAGGGGCTAAGGTATCAGTTCTCAAGATGAGCCTTT64440.1182205495714505No Hit
ATATTACTCTACATCTAGATGATCAAATAGTCCTTACTTATAACTAAACC64250.11787197873938074No Hit
GATTTGAGGTACTACGTGTTAGGAAATAAAGGCTCATCTTGAGAACTGAT63580.11664280791050315No Hit
GGGCTAAGGTATCAGTTCTCAAGATGAGCCTTTATTTCCTAACACGTAGT62110.1139459704202792No Hit
AATATTAACAGCCTGAAAAGGATTCTTGATTACTACCATTCATCAACTAT61900.11356070792167576No Hit
AACCAGGATATTACTCTACATCTAGATGATCAAATAGTCCTTACTTATAA61820.11341394125554112No Hit
CTTGTAAACCTGGAATAATGTTCCATTAACTGGATATGATTGCATAATTA60690.11134086209638938No Hit
TATTTATACCTGTCTTTCCACATACTTTTTTCAAATGGTTTCATGAAAAG60440.11088221626471863No Hit
ATTTTACTCAGTTCCCACAGAAGTACTCTTAAAAAAACCCAAACTTCCTT60400.11080883293165132No Hit
TCTCTAATGTATCAGCCACTGAGTCATCCTTCACATTGGAATTACTGATT60370.11075379543185083No Hit
AATGTATATAATTCCAAACTTCAATCAGTAATTCCAATGTGAAGGATGAC60180.11040522459978105No Hit
GGTATCAGTTCTCAAGATGAGCCTTTATTTCCTAACACGTAGTACCTCAA59240.10868071627269907No Hit
GAACAATTCAGAACATACAATATGTGTGTGATAACCACCTACTGGATGAA58120.10662598294681415No Hit
GAATGAAGGGTGACTGCAAATGAGCACAAAGATCATTCTGGGATGATGAA57230.10499320378606632No Hit
ACATACAATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCA56550.10374568712392188No Hit
ATTCTGGGATGATGAAAATGTTCTAAAAATAAACTGTGATGATAGCTGCA56540.10372734129065506No Hit
GTTCTTATACTATAGGAACAAAAACTACTGCCACAGAATGTAATGCTACT55250.10136072879923402No Hit
CTTGAGAACTGATACCTTAGCCCCTTGATGATGATTACAGAGAAATAAGG55090.10106719546696474No Hit
ATGATAAACTTGAGTTACACTGAATTTTACTCAGTTCCCACAGAAGTACT55080.10104884963369792No Hit
TGATCAAATAGTCCTTACTTATAACTAAACCACCTGAATCACCTATGATT54510.10000313713748864No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)