FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_B6a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_B6a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1006438
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTAAAGTGACTTTCCTGACGGGTTAGCTCTGAAGCCCTCTGTGGCTA90030.8945409453935562No Hit
CCCCCACCCCAGGCCATGTTAGGTTTAATCAGGACCTGGTTTTCAGTGAG69000.6855861960697033No Hit
CCCCAGGCCATGTTAGGTTTAATCAGGACCTGGTTTTCAGTGAGATGAAG68090.6765444071070449No Hit
ATGTTAGGTTTAATCAGGACCTGGTTTTCAGTGAGATGAAGGTTCAGGGA66830.6640250070049024No Hit
GCTCTGAAGCCCTCTGTGGCTATCAATGCCCAGCAGGAGTCGGTCCTCAA59210.5883124444824221No Hit
GGTTTAATCAGGACCTGGTTTTCAGTGAGATGAAGGTTCAGGGAAGTGAT54390.5404207710758139No Hit
AAGCAGGACTCTAAAGTGACTTTCCTGACGGGTTAGCTCTGAAGCCCTCT54260.5391290869382913No Hit
GGTTAAGTGTCTGCCTTCAGTTCAGGTCTTGATCTCCGGGTCCTGGGATG52850.5251192820620842No Hit
GTAGGTTAAGTGTCTGCCTTCAGTTCAGGTCTTGATCTCCGGGTCCTGGG50730.5040548945886384No Hit
GTTCAGGGAAGTGATTGACAAAAATGCTGTTTCTCCAGAGTGTTTGCTAC50710.5038561739520964No Hit
TCTCCAGAGTGTTTGCTACTTGAGGACCGACTCCTGCTGGGCATTGATAG47390.47086854828613384No Hit
GGTTAGCTCTGAAGCCCTCTGTGGCTATCAATGCCCAGCAGGAGTCGGTC44250.43966940834904883No Hit
GAGTAGCAGAGGGACAGGAAGCAGCAGACTCCCAGCTGAGCAGGGAGCCT43670.43390650988933244No Hit
GTCCTCAAGTAGCAAACACTCTGGAGAAACAGCATTTTTGTCAATCACTT42480.4220826320150869No Hit
GGTTTTCAGTGAGATGAAGGTTCAGGGAAGTGATTGACAAAAATGCTGTT40090.3983355159483247No Hit
AGCGAAAGCAGGACTCTAAAGTGACTTTCCTGACGGGTTAGCTCTGAAGC39550.3929700587616923No Hit
CTCTGGAGAAACAGCATTTTTGTCAATCACTTCCCTGAACCTTCATCTCA39530.3927713381251503No Hit
GATGAAGGTTCAGGGAAGTGATTGACAAAAATGCTGTTTCTCCAGAGTGT38960.3871077999837049No Hit
CTAAAGTGACTTTCCTGACGGGTTAGCTCTGAAGCCCTCTGTGGCTATCA38960.3871077999837049No Hit
AATCAGGACCTGGTTTTCAGTGAGATGAAGGTTCAGGGAAGTGATTGACA33420.3320621836615867No Hit
CTTGAGGACCGACTCCTGCTGGGCATTGATAGCCACAGAGGGCTTCAGAG31330.31129587714295365No Hit
CAGTAGGTTAAGTGTCTGCCTTCAGTTCAGGTCTTGATCTCCGGGTCCTG29890.29698799131193376No Hit
AGCGAAAGCAGGGGGAGTAGCAGAGGGACAGGAAGCAGCAGACTCCCAGC29670.2948020643099724No Hit
GGACTCTAAAGTGACTTTCCTGACGGGTTAGCTCTGAAGCCCTCTGTGGC27890.27711592765773946No Hit
GTGGCTATCAATGCCCAGCAGGAGTCGGTCCTCAAGTAGCAAACACTCTG26930.26757733710372616No Hit
CTCTGTGGCTATCAATGCCCAGCAGGAGTCGGTCCTCAAGTAGCAAACAC24490.24333341944560916No Hit
CTCTAAAGTGACTTTCCTGACGGGTTAGCTCTGAAGCCCTCTGTGGCTAT23610.23458971143776366No Hit
AGCAGGGGGAGTAGCAGAGGGACAGGAAGCAGCAGACTCCCAGCTGAGCA23580.23429163048295076No Hit
GGCCATGTTAGGTTTAATCAGGACCTGGTTTTCAGTGAGATGAAGGTTCA23450.23299994634542812No Hit
TGTTAGGTTTAATCAGGACCTGGTTTTCAGTGAGATGAAGGTTCAGGGAA23030.22882681297804733No Hit
GTTCAGGTCTTGATCTCCGGGTCCTGGGATGGAACCCCTTGTTAGGCTCC22950.22803193043187958No Hit
GGTTCAGGGAAGTGATTGACAAAAATGCTGTTTCTCCAGAGTGTTTGCTA22390.22246775260870516No Hit
GCAGGAGTCGGTCCTCAAGTAGCAAACACTCTGGAGAAACAGCATTTTTG22250.22107670815291158No Hit
AAAGCAGGGGGAGTAGCAGAGGGACAGGAAGCAGCAGACTCCCAGCTGAG22000.21859270019613727No Hit
GGTAAATATATTTTTAAAAACTTTACTCTGGGGCGCCTGGGTGGCTCAGT21730.21590997160282105No Hit
GTAGCAGAGGGACAGGAAGCAGCAGACTCCCAGCTGAGCAGGGAGCCTAA21720.21581061128455006No Hit
GGGTGGGGGAATGCCTGGGGGCTCAGTAGGTTAAGTGTCTGCCTTCAGTT21430.21292916205469192No Hit
GTAGCAAACACTCTGGAGAAACAGCATTTTTGTCAATCACTTCCCTGAAC21280.2114387572806273No Hit
GGGAGTAGCAGAGGGACAGGAAGCAGCAGACTCCCAGCTGAGCAGGGAGC21160.21024643346137564No Hit
GTGTTTGCTACTTGAGGACCGACTCCTGCTGGGCATTGATAGCCACAGAG20860.20726562391324652No Hit
GCAGAGGGACAGGAAGCAGCAGACTCCCAGCTGAGCAGGGAGCCTAACAA20490.20358929213722057No Hit
CCTCTGTGGCTATCAATGCCCAGCAGGAGTCGGTCCTCAAGTAGCAAACA20440.20309249054586573No Hit
AGCAAACACTCTGGAGAAACAGCATTTTTGTCAATCACTTCCCTGAACCT20000.19872063654194297No Hit
CTGAAGCCCTCTGTGGCTATCAATGCCCAGCAGGAGTCGGTCCTCAAGTA19130.19007628885236846No Hit
GGGAAGTGATTGACAAAAATGCTGTTTCTCCAGAGTGTTTGCTACTTGAG18460.18341914752821334No Hit
GGACCTGGTTTTCAGTGAGATGAAGGTTCAGGGAAGTGATTGACAAAAAT18250.18133258084452294No Hit
ACCCCAGGCCATGTTAGGTTTAATCAGGACCTGGTTTTCAGTGAGATGAA18100.17984217607045838No Hit
GCCTAACAAGGGGTTCCATCCCAGGACCCGGAGATCAAGACCTGAACTGA18080.17964345543391644No Hit
CTTCAGTTCAGGTCTTGATCTCCGGGTCCTGGGATGGAACCCCTTGTTAG18050.17934537447910354No Hit
TCAGGGAAGTGATTGACAAAAATGCTGTTTCTCCAGAGTGTTTGCTACTT17980.17864985225120672No Hit
CAGCAGGAGTCGGTCCTCAAGTAGCAAACACTCTGGAGAAACAGCATTTT17640.1752716014299937No Hit
CCTGGGGGCTCAGTAGGTTAAGTGTCTGCCTTCAGTTCAGGTCTTGATCT17620.17507288079345174No Hit
TTCCTGACGGGTTAGCTCTGAAGCCCTCTGTGGCTATCAATGCCCAGCAG16920.16811765851448374No Hit
GTTTGCTACTTGAGGACCGACTCCTGCTGGGCATTGATAGCCACAGAGGG16700.16593173151252238No Hit
GCCCAGCAGGAGTCGGTCCTCAAGTAGCAAACACTCTGGAGAAACAGCAT16420.16314964260093517No Hit
GTTTCTCCAGAGTGTTTGCTACTTGAGGACCGACTCCTGCTGGGCATTGA16300.1619573187816835No Hit
CAAGTAGGCAGAGAGGCAGGCAGAGAGAGAGAGGAGGAAGCAGGCTCCCT16170.1606656346441609No Hit
ACTCCCAGCTGAGCAGGGAGCCTAACAAGGGGTTCCATCCCAGGACCCGG15640.1553995377757994No Hit
CTGTGGCTATCAATGCCCAGCAGGAGTCGGTCCTCAAGTAGCAAACACTC15580.15480337586617357No Hit
ATCAATGCCCAGCAGGAGTCGGTCCTCAAGTAGCAAACACTCTGGAGAAA15280.15182256631804444No Hit
GGACAGGAAGCAGCAGACTCCCAGCTGAGCAGGGAGCCTAACAAGGGGTT14970.1487423964516443No Hit
TTGTTAGGCTCCCTGCTCAGCTGGGAGTCTGCTGCTTCCTGTCCCTCTGC14960.14864303613337335No Hit
ACTCTGGGGCGCCTGGGTGGCTCAGTGGGTTATGCCGCTGCCTTCGGCTC14840.14745071231412168No Hit
GGTCTTGATCTCCGGGTCCTGGGATGGAACCCCTTGTTAGGCTCCCTGCT14780.14685455040449585No Hit
CAAAAATGCTGTTTCTCCAGAGTGTTTGCTACTTGAGGACCGACTCCTGC14660.1456622265852442No Hit
CTCCCAGCTGAGCAGGGAGCCTAACAAGGGGTTCCATCCCAGGACCCGGA14510.14417182181117963No Hit
TCCCTGAACCTTCATCTCACTGAAAACCAGGTCCTGATTAAACCTAACAT14440.14347629958328284No Hit
GGGAATGCCTGGGGGCTCAGTAGGTTAAGTGTCTGCCTTCAGTTCAGGTC14090.13999868844379884No Hit
GTCCTGGGATGGAACCCCTTGTTAGGCTCCCTGCTCAGCTGGGAGTCTGC13910.13821020271492132No Hit
GGAAGCAGCAGACTCCCAGCTGAGCAGGGAGCCTAACAAGGGGTTCCATC13850.1376140408052955No Hit
GTCTTGATCTCCGGGTCCTGGGATGGAACCCCTTGTTAGGCTCCCTGCTC13850.1376140408052955No Hit
GCCTGGGGGCTCAGTAGGTTAAGTGTCTGCCTTCAGTTCAGGTCTTGATC13670.13582555507641803No Hit
CTCCAGAGTGTTTGCTACTTGAGGACCGACTCCTGCTGGGCATTGATAGC13600.13513003284852124No Hit
CCTGACGGGTTAGCTCTGAAGCCCTCTGTGGCTATCAATGCCCAGCAGGA13570.1348319518937083No Hit
GACAGAGAGAGACCACAAGTAGGCAGAGAGGCAGGCAGAGAGAGAGAGGA13480.13393770902926957No Hit
ACTTTACTCTGGGGCGCCTGGGTGGCTCAGTGGGTTATGCCGCTGCCTTC13440.13354026775618566No Hit
GGGTTATGCCGCTGCCTTCGGCTCGGGTCATGATCTCAGGGTCCTGGGAT13260.1317517820273082No Hit
GGCTATCAATGCCCAGCAGGAGTCGGTCCTCAAGTAGCAAACACTCTGGA12830.12747928834165642No Hit
ATCAGGACCTGGTTTTCAGTGAGATGAAGGTTCAGGGAAGTGATTGACAA12490.12410103752044338No Hit
GCCTTCAGTTCAGGTCTTGATCTCCGGGTCCTGGGATGGAACCCCTTGTT12340.12261063274637882No Hit
TCCCAGGACCCGGAGATCAAGACCTGAACTGAAGGCAGACACTTAACCTA12200.12121958829058521No Hit
TAACAAGGGGTTCCATCCCAGGACCCGGAGATCAAGACCTGAACTGAAGG12170.1209215073357723No Hit
GTTAAGTGTCTGCCTTCAGTTCAGGTCTTGATCTCCGGGTCCTGGGATGG12010.11933174224343676No Hit
GCAGGGGGAGTAGCAGAGGGACAGGAAGCAGCAGACTCCCAGCTGAGCAG11680.11605285174049469No Hit
GTGTCTGCCTTCAGTTCAGGTCTTGATCTCCGGGTCCTGGGATGGAACCC11580.11505924855778499No Hit
GCGAAAGCAGGACTCTAAAGTGACTTTCCTGACGGGTTAGCTCTGAAGCC11230.11158163741830099No Hit
GGCAGAGGGAGAGGGGGAAGCAGGCTTCGGGCTGAGCAAGGAGCCGGATA11200.11128355646348807No Hit
GTGACTTTCCTGACGGGTTAGCTCTGAAGCCCTCTGTGGCTATCAATGCC11140.11068739455386224No Hit
GGGGAATGCCTGGGGGCTCAGTAGGTTAAGTGTCTGCCTTCAGTTCAGGT10980.1090976294615267No Hit
GCTGAGCAGGGAGCCTAACAAGGGGTTCCATCCCAGGACCCGGAGATCAA10930.10860082787017182No Hit
AGGTTAAGTGTCTGCCTTCAGTTCAGGTCTTGATCTCCGGGTCCTGGGAT10790.10720978341437824No Hit
GGTCATGATCTCAGGGTCCTGGGATCGAGTCCCGCATCGGGCTCTTTGCT10560.10492449609414589No Hit
GGAGAAACAGCATTTTTGTCAATCACTTCCCTGAACCTTCATCTCACTGA10420.10353345163835229No Hit
GGGGGAGTAGCAGAGGGACAGGAAGCAGCAGACTCCCAGCTGAGCAGGGA10270.10204304686428772No Hit
GAGCCTAACAAGGGGTTCCATCCCAGGACCCGGAGATCAAGACCTGAACT10170.10104944368157799No Hit
GTTCCATCCCAGGACCCGGAGATCAAGACCTGAACTGAAGGCAGACACTT10150.10085072304503606No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)