FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_B2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_B2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6921426
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT446350.6448815605339131No Hit
GGCATAGGGAGTGTCCTTATTGCTAAAAAGGATTCTGTCTCCTTCAAAGA431760.6238020893382376No Hit
AACAAGTACTCTGGACCAAAAATAAAACTTCTTTGAAGGAGACAGAATCC270850.3913210948148546No Hit
AGCAGGGACAGAGCTGAGAAACTGATCTCTGCTCTCTCCCTGCCCAGTCC264040.38148208187156807No Hit
AGTCAGGAAACAAGTACTCTGGACCAAAAATAAAACTTCTTTGAAGGAGA263230.3803118027990186No Hit
CCCTATGCCATTGGGCAAGGGGCATTAGCCTGCAGAAAGGATCTTGCTGT255030.36846453317567796No Hit
TAACTATACTGTTAGAGTGAAGTTCAAGTATTCCACGCTTTTCTAGCAAG242180.34989899480251613No Hit
AAACAAGGGAGTTTGGAGCCATAGGCATAGTAAAAGCTGGGATTATGGGC239100.3454490447488711No Hit
CCTTAGTACAACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCC235380.34007442974901414No Hit
GTATAACTATACTGTTAGAGTGAAGTTCAAGTATTCCACGCTTTTCTAGC225870.32633448656389596No Hit
GTCCTGGGTGGCAGTCCTATCGGGGCACCATAGGGGCAATTGCAGCCTGT225800.326233351335404No Hit
TCTATACACTGAAAACTAGAAAACACTGATGAAAGAAATTGAAGATGGCA222500.32146554770649866No Hit
CAAGTACTCTGGACCAAAAATAAAACTTCTTTGAAGGAGACAGAATCCTT206860.2988690480834441No Hit
ATGTAAAGAAGCTTAAAACCACTTACCTAAAGTCTTGCTTTATAATCAGT204300.29517038829859626No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA191580.27679267249263373No Hit
CTCCCTGCCCAGTCCTCTTCCCATCCTCATCCACAGGCTGCAATTGCCCC190510.2752467482856856No Hit
ACTATACTGTTAGAGTGAAGTTCAAGTATTCCACGCTTTTCTAGCAAGCA187880.27144695327234586No Hit
GAACTAGAACAAATAATCCTAAAATTTGTATGGAACCACAAAAGAGCCTG183370.2649309549795086No Hit
AATCTATACACTGAAAACTAGAAAACACTGATGAAAGAAATTGAAGATGG177750.2568112409205848No Hit
GCATAAACTATCTGGTGTTTTTAAAATCTGCAATATTGAGATGGAGAACT162960.23544281192921807No Hit
AAATAAAACTTCTTTGAAGGAGACAGAATCCTTTTTAGCAATAAGGACAC158600.22914353198314913No Hit
GCCATAGGCATAGTAAAAGCTGGGATTATGGGCCACAGTCCTGGGTGGCA156620.2262828498058059No Hit
GCATAAAGCCAAGTAACAAATTGCTACCTTCAACAAGCAGAGCTAGACTC154680.22347995918760097No Hit
TAGTACAACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCCAGA152610.22048924600219666No Hit
GTTTGGAGCCATAGGCATAGTAAAAGCTGGGATTATGGGCCACAGTCCTG151450.2188132907871875No Hit
TTTCTGATGAGAGTGAGCACTGGTTTCACTGGTTTTTCATGATGTAAAGA141670.2046832545778861No Hit
GAATAAAACTGTCAAAAAATAAAGAAAGTGACTTAGAGCAGGATCCATAA140430.20289171624460045No Hit
CTGTATAACTATACTGTTAGAGTGAAGTTCAAGTATTCCACGCTTTTCTA140140.20247272744084815No Hit
ATATTGAGATGGAGAACTGATTATAAAGCAAGACTTTAGGTAAGTGGTTT128860.18617550776386255No Hit
GAGCTGAGAAACTGATCTCTGCTCTCTCCCTGCCCAGTCCTCTTCCCATC128690.185929893637525No Hit
TCTTTACATCATGAAAAACCAGTGAAACCAGTGCTCACTCTCATCAGAAA126970.18344485659458037No Hit
GCATAGGGAGTGTCCTTATTGCTAAAAAGGATTCTGTCTCCTTCAAAGAA125770.18171110982043295No Hit
GGAAACAAGTACTCTGGACCAAAAATAAAACTTCTTTGAAGGAGACAGAA125540.18137880835538803No Hit
ACTTAGAGCAGGATCCATAAATGGGCTTCTGGTCTCTTAATCTCTGAAAC124880.18042524762960696No Hit
AGTAGAAACAAGGGAGTTTGGAGCCATAGGCATAGTAAAAGCTGGGATTA124570.17997736304628556No Hit
GTCCTCTTCCCATCCTCATCCACAGGCTGCAATTGCCCCTATGGTGCCCC123040.1777668359092476No Hit
ACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCCAGATTTGCTT122330.17674103573454372No Hit
AGCATAAACTATCTGGTGTTTTTAAAATCTGCAATATTGAGATGGAGAAC121830.17601864124531563No Hit
CCCCCAAAATGCATAAAGCCAAGTAACAAATTGCTACCTTCAACAAGCAG121360.17533959042544123No Hit
CTCTGAAACTGTACGCAAATTTGCATAATGTGCATTTTTCTGATGAGAGT121060.17490615373190438No Hit
CCCCTATGGTGCCCCGATAGGACTGCCACCCAGGACTGTGGCCCATAATC119520.17268117870508187No Hit
CCATAGGGGCAATTGCAGCCTGTGGATGAGGATGGGAAGAGGACTGGGCA118900.17178540953843904No Hit
GTATTTCTTTCTCAGTGTCATGAATTGTGGTTAATGCTTGCTAGAAAAGC114670.16567395215956943No Hit
ACCCAGGACTGTGGCCCATAATCCCAGCTTTTACTATGCCTATGGCTCCA111810.16154185568118476No Hit
ACCAATAACACTTCTCACAGAACTAGAACAAATAATCCTAAAATTTGTAT110990.16035712871885072No Hit
GAGCTAGACTCGGTTTGCAGTTCTATCCTAAAACTCCTTTTCACCAAGCT109280.15788653956569065No Hit
GGGCTAGCTGGACGAGTCAGGAAACAAGTACTCTGGACCAAAAATAAAAC107490.1553003672942541No Hit
GTATGTTTGTTTTGACTCCTTTTAAAAAGAGTAGAGCATAAACTATCTGG107030.15463576436416426No Hit
GACTTAATATGTTGCAGAGAAGGTAGTGTTAATGAACCGAAGTTAGAGGT106720.15418787978084283No Hit
GGTCTGAGAAACGCAGGTGCCTAAGTTCCAGTCACCCCCAAAATGCATAA105550.15249747667604913No Hit
GTACAACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCCAGATT104740.1513271976034996No Hit
CTTCCCATCCTCATCCACAGGCTGCAATTGCCCCTATGGTGCCCCGATAG103790.14995464807396625No Hit
GTCATGAAGGCACCCCTTAGTACAACTTTGTAGTATAACTTGAAGTCTGG103620.14970903394762872No Hit
ACTCTGGACCAAAAATAAAACTTCTTTGAAGGAGACAGAATCCTTTTTAG103480.14950676349064485No Hit
GTTCCAGTCACCCCCAAAATGCATAAAGCCAAGTAACAAATTGCTACCTT102870.1486254422137866No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT101640.1468483517702855No Hit
GTAACAAATTGCTACCTTCAACAAGCAGAGCTAGACTCGGTTTGCAGTTC101620.14681945599071639No Hit
GGTTAGGCCTTTAAGCTAAGCTTGGTGAAAAGGAGTTTTAGGATAGAACT99370.14356868078918997No Hit
GAGTAGAGCATAAACTATCTGGTGTTTTTAAAATCTGCAATATTGAGATG98390.14215278759030292No Hit
AGCTGGACGAGTCAGGAAACAAGTACTCTGGACCAAAAATAAAACTTCTT97940.14150263254999765No Hit
TTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCCAGATTTGCTTTCC96870.13995670834304955No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC96500.13942213642102075No Hit
GGACAGAGCTGAGAAACTGATCTCTGCTCTCTCCCTGCCCAGTCCTCTTC95560.13806403478127194No Hit
GAGAAACGCAGGTGCCTAAGTTCCAGTCACCCCCAAAATGCATAAAGCCA95510.13799179533234915No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA92290.13333957482172026No Hit
CTAGAAAACACTGATGAAAGAAATTGAAGATGGCACAAACAAACGGGAAG91690.13247270143464657No Hit
GTCATGAATTGTGGTTAATGCTTGCTAGAAAAGCGTGGAATACTTGAACT89900.12988652916321003No Hit
TAGTAAAAGCTGGGATTATGGGCCACAGTCCTGGGTGGCAGTCCTATCGG89090.1287162500906605No Hit
GGCATAGTAAAAGCTGGGATTATGGGCCACAGTCCTGGGTGGCAGTCCTA88780.12826836550733908No Hit
CTCTTAATCTCTGAAACTGTACGCAAATTTGCATAATGTGCATTTTTCTG88120.12731480478155802No Hit
ATCAAAATACCAATAACACTTCTCACAGAACTAGAACAAATAATCCTAAA87810.1268669201982366No Hit
GAGCATAAACTATCTGGTGTTTTTAAAATCTGCAATATTGAGATGGAGAA87140.12589891158267097No Hit
GTAGAAACAAGGGAGTTTGGAGCCATAGGCATAGTAAAAGCTGGGATTAT85740.12387620701283233No Hit
ATGGAGAACTGATTATAAAGCAAGACTTTAGGTAAGTGGTTTTAAGCTTC85520.12355835343757196No Hit
ATGGCATAGGGAGTGTCCTTATTGCTAAAAAGGATTCTGTCTCCTTCAAA84570.1221858039080386No Hit
TTGCAGTTCTATCCTAAAACTCCTTTTCACCAAGCTTAGCTTAAAGGCCT82650.11941180906940274No Hit
CCATAAATGGGCTTCTGGTCTCTTAATCTCTGAAACTGTACGCAAATTTG82370.11900726815543503No Hit
AGCGAAAGCAGGGCTAGCTGGACGAGTCAGGAAACAAGTACTCTGGACCA82160.11870386246995923No Hit
CCACAAAAGAGCCTGAATAGCCAAACAACCTTAAAAAAGGAAAGCAAATC82070.11857383146189816No Hit
CTGTTAGAGTGAAGTTCAAGTATTCCACGCTTTTCTAGCAAGCATTAACC82030.11851603990275991No Hit
CTTAATGACTGAGTCATGAAGGCACCCCTTAGTACAACTTTGTAGTATAA81630.11793812431137746No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT80930.11692677202645814No Hit
TTATAAAGCAAGACTTTAGGTAAGTGGTTTTAAGCTTCTTTACATCATGA80250.11594431552110793No Hit
CAATAACACTTCTCACAGAACTAGAACAAATAATCCTAAAATTTGTATGG79090.11426836030609877No Hit
TCCTAGTGATTAGGAAGTTCAGACTGTATTTCTTTCTCAGTGTCATGAAT78470.11337259113945594No Hit
TAACAATATTGTTCTTCCAATCCATGAGCATGGATTGTCTTCCCGTTTGT78260.11306918545398015No Hit
GCTAAAAAGGATTCTGTCTCCTTCAAAGAAGTTTTATTTTTGGTCCAGAG77170.11149436546746291No Hit
GTATAACTTGAAGTCTGGAACTTGATACCTCCAGATTTGCTTTCCTTTTT75480.10905267209387198No Hit
AATTTGCATAATGTGCATTTTTCTGATGAGAGTGAGCACTGGTTTCACTG75350.10886484952667268No Hit
CCAATAACACTTCTCACAGAACTAGAACAAATAATCCTAAAATTTGTATG74700.10792573669067616No Hit
GCCCAATGGCATAGGGAGTGTCCTTATTGCTAAAAAGGATTCTGTCTCCT74400.10749229999713932No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA74030.10695772807511053No Hit
AAGCTAAGCTTGGTGAAAAGGAGTTTTAGGATAGAACTGCAAACCGAGTC73570.1062931251450207No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT73420.10607640679825227No Hit
ATCCATAAATGGGCTTCTGGTCTCTTAATCTCTGAAACTGTACGCAAATT73300.10590303212083753No Hit
AGATATCCCTTATAGGAAAAACCTCTAACTTCGGTTCATTAACACTACCT72570.10484833616656451No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA72500.10474720093807258No Hit
GTCCTTATTGCTAAAAAGGATTCTGTCTCCTTCAAAGAAGTTTTATTTTT71090.10271004847844938No Hit
CCTTTAAGCTAAGCTTGGTGAAAAGGAGTTTTAGGATAGAACTGCAAACC71020.10260891324995744No Hit
AGTAAAAGCTGGGATTATGGGCCACAGTCCTGGGTGGCAGTCCTATCGGG70170.1013808426182697No Hit
GTAAAAGCTGGGATTATGGGCCACAGTCCTGGGTGGCAGTCCTATCGGGG70030.10117857216128583No Hit
GCAATATTGAGATGGAGAACTGATTATAAAGCAAGACTTTAGGTAAGTGG69580.10052841712098057No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)