FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_B1a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_B1a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1316061
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAATGAAATGTTCAAATAAGGAAAGATGAAGTTCATATGGTGCATGG37590.2856250584129459No Hit
TCCTAATACAGTTCCGATCTTATTAGTTAAGACAGAATCTGTCTGTTCCA35590.2704281944377958No Hit
ATCTAGAAGTCTATTCCTCAAAAGTATAAGATTAGCAGATACAAGACCTC35290.2681486648415233No Hit
TCATTGAACTGCTCACTGAGTCCAGTGAAGCTTCAAAGAATGCCAAATTA34450.26176598197196027No Hit
GTTTATTTCTTCTACTTTACCAACGTTTGCAGTTTGTGAGAAATTTGTAG34410.26146204469245726No Hit
GTATAAGATTAGCAGATACAAGACCTCCAAACTCTTCTTCAGTTCCAGTA32020.2433017922421529No Hit
CCATATGAACTTCATCTTTCCTTATTTGAACATTTCATTGAACTGCTCAC29400.2233939004347063No Hit
TCACTGGACTCAGTGAGCAGTTCAATGAAATGTTCAAATAAGGAAAGATG28510.21663129596576453No Hit
CTGTTGATAGTGGGCATGAGACCTCCATTATTCCAAATTCAACTGCTTTC27370.20796908349992896No Hit
GTCCAGTGAAGCTTCAAAGAATGCCAAATTAATGAGGGAATTCCAGCTAA27350.20781711486017748No Hit
GCATACATACGGCCTGAAAGCTTCCAACATAATTTGGTCCTAGTTCATAA26530.2015864006303659No Hit
CTTTAGATGTGTAAACTAGTTTTAGCAAGATATCTAGAAGTCTATTCCTC26050.19793915327632988No Hit
ATACAAGACCTCCAAACTCTTCTTCAGTTCCAGTATCAAGCTTCTCTTCA24630.18714937985397334No Hit
GAAATAAACAATGAAGAGAAGCTTGATACTGGAACTGAAGAAGAGTTTGG24070.1828942579409313No Hit
GTCTAAGGGAAAGCCGTGACCCCGGGGTCCAGCAGGTGCCTGCCCTGTGC24010.1824383520216768No Hit
CTTCTAGATATCTTGCTAAAACTAGTTTACACATCTAAAGAAAAGACAAG23690.1800068537856528No Hit
TCCATAAACATCATAATCCAGTCAAAACCCAGTGTCCTGACCAGTTCTTC23580.17917102626701956No Hit
GTTTATTTCCATAACTACAAATTTCTCACAAACTGCAAACGTTGGTAAAG23180.17613165347198953No Hit
ACTCATAGCAGCAATGGTAGGTTGGGATAAAGACATATCTCGAAGAGTCA22620.1718765315589475No Hit
GTTCTTCACAAGCTTGCAAATTAATGCTTGTCTTTTCTTTAGATGTGTAA21720.16503794277012995No Hit
CCATTATTCCAAATTCAACTGCTTTCCAGGATCTACTTTGTGATTTTGAA20640.15683163622354893No Hit
CTATCAACAGTTCCCACCAAGGAAAATGTTAGATGGAGAATGTGGCTGTT20480.15561588710553692No Hit
TATGTATGCCGTGTAAAGATACAAAGTCTGAAGGCGTAGTCCCATCTCCT20300.15424816934777338No Hit
GAATAAGCCTGGCCTCAGTTTTTCTGACGCGACAGAGGTTTTGGGCACTC20000.15196863975150088No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT19190.1458139098415651No Hit
ATCTTATACTTTTGAGGAATAGACTTCTAGATATCTTGCTAAAACTAGTT19070.1449020980030561No Hit
GTCCTGACCAGTTCTTCACAAGCTTGCAAATTAATGCTTGTCTTTTCTTT19040.14467414504342885No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT18660.14178674088815033No Hit
GTCTATGCGTACATTGGCACTCCACCTGCCCAGCGTCAAATTGCTTCCTT18590.14125485064902008No Hit
GTGTAAACTAGTTTTAGCAAGATATCTAGAAGTCTATTCCTCAAAAGTAT18390.13973516425150506No Hit
GGATAAAGACATATCTCGAAGAGTCAGTAGCAATTTGGGGATTAGCTGGA18150.13791154057448704No Hit
GTCTTGTATCTGCTAATCTTATACTTTTGAGGAATAGACTTCTAGATATC17700.1344922461800783No Hit
GCTCAGGTTTGGCCAGTTTATTTCTTCTACTTTACCAACGTTTGCAGTTT17640.13403634026082378No Hit
TTACCAACGTTTGCAGTTTGTGAGAAATTTGTAGTTATGGAAATAAACAA17110.130009171307409No Hit
ACTATAAGTTCTCCACATCGAAAGCGGGCACAGCACGGGAGAATCTCATA16990.1290973594689No Hit
GTTCATATGGTGCATGGAGCCAAACTTCAAAATCACAAAGTAGATCCTGG16740.12719775147200624No Hit
CTCCTTAACAGCCACATTCTCCATCTAACATTTTCCTTGGTGGGAACTGT16560.12583003371424273No Hit
AATTAATGCTTGTCTTTTCTTTAGATGTGTAAACTAGTTTTAGCAAGATA16520.12552609643473972No Hit
GCACGGGAGAATCTCATAAAAAGAAGATTCTTCACTAAAATCATCAACAT16300.12385444139747322No Hit
GTATCAAGCTTCTCTTCATTGTTTATTTCCATAACTACAAATTTCTCACA16130.12256270795958546No Hit
AACCTGAGCAGATCATTGCTGTTGGGAAAACCTTGTAGTAAGAAGCTCAG16060.12203081772045521No Hit
ATATGAACTTCATCTTTCCTTATTTGAACATTTCATTGAACTGCTCACTG15970.12134695884157345No Hit
GATATCTAGAAGTCTATTCCTCAAAAGTATAAGATTAGCAGATACAAGAC15970.12134695884157345No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA15640.11883947628567369No Hit
GGTTTGGCCAGTTTATTTCTTCTACTTTACCAACGTTTGCAGTTTGTGAG15570.11830758604654344No Hit
GGATAGACTGATTACTCAGTAGCACAACAAGAATCCTCATGGCTGCCGTA15400.11701585260865568No Hit
CATCTAAAGAAAAGACAAGCATTAATTTGCAAGCTTGTGAAGAACTGGTC15360.11671191532915268No Hit
AGTTTACACATCTAAAGAAAAGACAAGCATTAATTTGCAAGCTTGTGAAG15260.11595207213039517No Hit
CGCATAGACTGTGCTGACCACAGGTGGGTTAGCGGAACCTGAGCCACCAG15190.11542018189126492No Hit
TCATAATCCAGTCAAAACCCAGTGTCCTGACCAGTTCTTCACAAGCTTGC15170.11526821325151342No Hit
GAGTAATGTGCTGAGCTTCTTACTACAAGGTTTTCCCAACAGCAATGATC14580.11078513837884413No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG14570.11070915405896839No Hit
GATTAGCAGATACAAGACCTCCAAACTCTTCTTCAGTTCCAGTATCAAGC14230.10812568718319288No Hit
CTAGATATCTTGCTAAAACTAGTTTACACATCTAAAGAAAAGACAAGCAT14230.10812568718319288No Hit
GCTATGAGTAATGTGCTGAGCTTCTTACTACAAGGTTTTCCCAACAGCAA14090.10706190670493236No Hit
TCTTACTACAAGGTTTTCCCAACAGCAATGATCTGCTCAGGTTTGGCCAG13940.10592214190679611No Hit
GTATTAGGATTCAATGTGGGCAGGAGTGCTGGCGGGAGATCGACGGTCAG13760.1045544241490326No Hit
GCATTACGTGTGCCTTGCCATTGTCTTATCCGCAAAAGATCGATCTCTGA13760.1045544241490326No Hit
GTTGATAGTGGGCATGAGACCTCCATTATTCCAAATTCAACTGCTTTCCA13690.10402253390990235No Hit
ATGGTAGGTTGGGATAAAGACATATCTCGAAGAGTCAGTAGCAATTTGGG13590.10326269071114486No Hit
CCTCAAAAGTATAAGATTAGCAGATACAAGACCTCCAAACTCTTCTTCAG13470.10235087887263583No Hit
GAAATAAACTGGCCAAACCTGAGCAGATCATTGCTGTTGGGAAAACCTTG13410.10189497295338135No Hit
GTTTTGGAGTAGTTCCTCTTTGGTGGAAACAATCAGAGATCGATCTTTTG13370.10159103567387834No Hit
TCTAGATATCTTGCTAAAACTAGTTTACACATCTAAAGAAAAGACAAGCA13240.10060323951549359No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)