FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_B11a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_B11a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences767459
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT406135.291878784404118No Hit
GCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAG224522.925498300234931No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC189102.463975274249178No Hit
CCTATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAAC139341.8156018758005315No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC112211.4620976495161304No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA111221.4491979376096964No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC106641.3895204825274055No Hit
AGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTA98581.2844985855921944No Hit
ATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAACTAC83251.0847485012228666No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTATTC73910.9630481888934783No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT61630.8030396412055888No Hit
GTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGT56600.7374986807112823No Hit
AATGAAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATG56230.732677576261403No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA53450.6964541428271739No Hit
GTATATAGCAGAACTACTAGTATTGCCTTCATTTTGGTTGTTTTTATTTT53060.6913724381367604No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA53040.6911118378962263No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG52940.6898088366935563No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA52730.687072534167949No Hit
ATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCATG46330.603680457197062No Hit
ATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATT43230.5632874199142885No Hit
ATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCC42770.5572936143820061No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAACTACTAGTATTG42670.555990613179336No Hit
GAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAATAGTTTAAAAA41830.545045403076907No Hit
CTTAAATACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAA39860.5193762793843059No Hit
GTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGG35290.4598291244222818No Hit
ACCAAAATGAAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGC35040.45657162141560653No Hit
TACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATT34840.45396561901026633No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC32620.42503899231098985No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAATTCG32060.41774218557603726No Hit
CATGATAACCTATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATAT31810.414484682569362No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC31570.4113574796829537No Hit
TAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGT30350.3954608650103784No Hit
GTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAA28810.37539464648925874No Hit
CTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTA27580.3593677316964164No Hit
AGCGAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTA27280.355458728088406No Hit
TACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAACTACT26960.35128912423986164No Hit
GTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTG26920.3507679237587936No Hit
CTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATG26820.34946492255612355No Hit
GTAGAATTGTTCGCATGATAACCTATACATAATGTGTCTGCATTTGCGGT26640.3471195203913173No Hit
AAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAGTTCT26330.34308021666303995No Hit
GAATTGTTCGCATGATAACCTATACATAATGTGTCTGCATTTGCGGTTGC25820.3364349105294224No Hit
GAATAGATCGCATGATAACCTATACATAATGTGTCTGCATTTGCGGTTGC25780.33591371004835435No Hit
GTTGAATTGTTCGCATGATAACCTATACATAATGTGTCTGCATTTGCGGT25760.3356531098078203No Hit
CATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACC25690.33474100896595127No Hit
ATGTATAGGTTATCATGCGATCTATTCAACTGTCGCCAGTTCATTGGTAC25450.331613806079543No Hit
GAGCGAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT25440.331483505959276No Hit
AACCTATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGA25340.3301805047566059No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAA23270.3032083798613346No Hit
CAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACA23030.3000811769749263No Hit
GTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGCTTTAC22600.2944782718034449No Hit
GTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGC22450.2925237699994397No Hit
GGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACA22060.2874420653090263No Hit
CATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAACTACTAG22030.28705116494822525No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAACTACTAGTATTGCCTTC21980.2863996643468902No Hit
GTAGAGACCCATTAGAGCACATCCAGAACTACTAGTATTGCCTTCATTTT21660.28223006049834587No Hit
GGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAGTTCTGCTAT21540.2806664590551417No Hit
AATAAAAACAACCAAAATGAAGGCAATACTAGTAGTTCTGCTATATACAT21250.27688775556739836No Hit
ATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCATGCG21170.27584535460526227No Hit
AGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGAC21110.2750635538836603No Hit
CACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACA20990.2734999524404561No Hit
AAATGAAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAAT20400.26581224534470244No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAATTCGGA19170.24978533055186009No Hit
CTGTAGAATTGTTCGCATGATAACCTATACATAATGTGTCTGCATTTGCG18660.24314002441824253No Hit
ACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTAT17230.22450710722005995No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA16540.21551639892163618No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA16400.21369219723789806No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCAT16310.21251949615549492No Hit
CTTAAATACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAC15990.20834989230695058No Hit
CTCTTAGTTTGCATAGTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACA15930.20756809158534853No Hit
ATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAACTACTAGTAT15830.20626509038267843No Hit
GTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTACTA15120.19701378184372062No Hit
CAATATGGAGAGAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAA15000.19545018040051648No Hit
GTTGAATAGATCGCATGATAACCTATACATAATGTGTCTGCATTTGCGGT14850.19349567859651134No Hit
ATGTTACATTTACCCAAATGCAATGGGGCTACCCCTCTTAGTTTGCATAG14820.19310477823571032No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA14560.18971697510876803No Hit
CTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATA14410.18776247330476284No Hit
ATTCAATATGGAGAGAATAAAAGAATTCGGATGGCCATCAATTAGTTGTC14360.1871109727034278No Hit
GTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACAGAGTGTGTTACTGTT14290.18619887186155873No Hit
CTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGA14260.1858079715007577No Hit
TCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGGCG14130.18411406993728657No Hit
TAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATA13980.18215956813328138No Hit
ATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAG13640.177729364044203No Hit
CTGTTGAATTGTTCGCATGATAACCTATACATAATGTGTCTGCATTTGCG13580.17694756332260095No Hit
TACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAACTACTAG12730.16587205309990502No Hit
CATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGC12540.1633963508148318No Hit
CTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAAC12520.16313575057429777No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC12310.16039944804869055No Hit
CATTATGTATAGGTTATCATGCGATCTATTCAACTGTCGCCAGTTCATTG12150.15831464612441837No Hit
AAATAAAAACAACCAAAATGAAGGCAATACTAGTAGTTCTGCTATATACA12100.15766314552308333No Hit
GTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAG11950.15570864371907814No Hit
CTACTAGTATTGCCTTCATTTTGGTTGTTTTTATTTTCCCCTGCTTTTGC11700.15245114071240287No Hit
ATTCAACAGACACTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACA11320.14749973614225648No Hit
TGATAACCTATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAG11030.14372103265451314No Hit
GAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGT10760.14020292940730383No Hit
GTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGTGTCTGCATT10450.1361636256790265No Hit
CCTATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCCTGTC10300.13420912387502132No Hit
CAACCAAAATGAAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACC10300.13420912387502132No Hit
TTTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCT10260.1336879233939533No Hit
GCGAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAG10260.1336879233939533No Hit
AATGTATATAGCAGAACTACTAGTATTGCCTTCATTTTGGTTGTTTTTAT10220.13316672291288525No Hit
GTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT10120.13186372171021515No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGCTGTCT10050.13095162086834605No Hit
GTACCAATGAACTGGCGACAGTTGAATAGATCGCATGATAACCTATACAT9880.12873651882380688No Hit
CTTTTATTCTCTCCATATTGAATATAATTGACCTGCTTTTGCTCCTGTCT9840.12821531834273883No Hit
GGTCAATTATATTCAATATGGAGAGAATAAAAGAATTCGGATGGCCATCA9800.1276941178616708No Hit
CAGTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGCTTT9560.12456691497526252No Hit
GAACTACTAGTATTGCCTTCATTTTGGTTGTTTTTATTTTCCCCTGCTTT9480.12352451401312643No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT9350.1218306124496553No Hit
CAGTTGAATAGATCGCATGATAACCTATACATAATGTGTCTGCATTTGCG9280.12091851160778622No Hit
TGCAGACACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTG9270.1207882114875192No Hit
GGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTACTAGAAAA9200.11987611064565012No Hit
AGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGTA9160.11935491016458208No Hit
ACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCA9100.11857310944298001No Hit
TAAATACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACT9010.11740040836057691No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGCTGTC8850.11531560643630474No Hit
GTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACAT8840.11518530631603772No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGA8720.1136217048728336No Hit
CATCCAGAACTACTAGTATTGCCTTCATTTTGGTTGTTTTTATTTTCCCC8690.11323080451203257No Hit
TAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAACTACTAGTA8640.11257930391069752No Hit
GTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGTGT8540.1112763027080274No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG8520.11101570246749339No Hit
CTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAG8460.11023390174589132No Hit
ATGTATAGGTTATCATGCGAACAATTCAACTGTCGCCAGTTCATTGGTAC8430.10984300138509027No Hit
CAATTCAACAGACACTGTAGACACAGTACTAGAAAAGAATGTAACAGTAA8280.10788849958108511No Hit
CTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTATTCTCTCCATA8150.10619459801761398No Hit
CCGTTATGCTTGTCTTCTAGAAGGTTAACAGAGTGTGTTACTGTTACATT8140.10606429789734696No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGGAGGCAATACTAGT8050.10489159681494385No Hit
CATTAGAGCACATCCAGAACTACTAGTATTGCCTTCATTTTGGTTGTTTT8010.10437039633387582No Hit
TCTAGAAGGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGT7970.10384919585280777No Hit
ATTATATTCAATATGGAGAGAATAAAAGAATTCGGATGGCCATCAATTAG7870.10254619465013767No Hit
GTCTTCTAGAAGGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTA7790.10150379368800157No Hit
GTTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACC7780.10137349356773456No Hit
CTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTC7770.10124319344746754No Hit
GACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAG7690.10020079248533147No Hit
ATATAGCAGAACTACTAGTATTGCCTTCATTTTGGTTGTTTTTATTTTCC7690.10020079248533147No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)