FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_B11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_B11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6044201
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT224130.37081824380095896No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC216090.35751623746463757No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT173290.28670456194292676No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT151090.24997514146203942No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT148150.24511097496592188No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA138310.22883090750952856No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC136620.226034839013461No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA134720.22289133005338507No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT124840.2065450834609901No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA123310.20401373150892899No Hit
ACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACC118370.19584060821273153No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC110960.1835809232684353No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA109420.18103302653237374No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT107410.17770752494829342No Hit
GTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGT105980.17534162083623625No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT104110.17224774622816152No Hit
GATTGAAAGCGACAGAGAATAGTTTCGAACAAATAACATTTATGCAAGCC98400.16280067456393327No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT95060.15727471670779974No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA93190.15418084209972502No Hit
ATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA92770.1534859611717082No Hit
GAAAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATT92640.15327087897970304No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG91060.15065680310763988No Hit
GAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGA88710.14676877886754594No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA87310.14445250910748997No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA86570.1432281950914604No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT85250.14104428360340762No Hit
CTTCTAGGTATTTATTTCTTCTCTCATCAAAAGCAGAAAGCACCATCCTC84840.14036594745939124No Hit
GAAATAAGGTGGTTAATTGAAGAAATGCGGCACAGATTGAAAGCGACAGA82630.1367095501953029No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC79610.1317130254271822No Hit
ATGTTACATTTACCCAAATGCAATGGGGCTACCCCTCTTAGTTTGCATAG79230.13108432363516698No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA79080.130836151875161No Hit
ATCCAGAATAGCATAACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGA78720.13024053965114663No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG78000.12904931520311783No Hit
ATATCATTGGGATCTTGCACCTGATATTGTGGATTACTGATCGTCTTTTT77610.12840406862710224No Hit
GTAATGGACAGGCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATA77480.12818898643509705No Hit
ATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG76480.12653450803505709No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC75390.1247311265790135No Hit
GTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACCTTGAT74990.1240693352189975No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT74050.12251412552295995No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT73210.12112436366692636No Hit
CTCTTAGTTTGCATAGTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACA72940.12067765449891557No Hit
GTCCATTACTTGGTCCATCGGTCATTACAGTAAAGCAAGAACCATTTACA72370.1197346018108928No Hit
GAATAGTTTCGAACAAATAACATTTATGCAAGCCTTACAACTACTGCTTG71830.11884118347487121No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT71440.1181959368988556No Hit
GTGTAATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACT70920.11733560813083482No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT70060.11591275670680046No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT69970.11576385365079686No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA69020.11419209917075887No Hit
GATCAAGAAAGAAGAGTTCTCTGAGATCATGAAGATCTGTTCCACCATTG68910.11401010654675449No Hit
CTTCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGG68380.1131332329947333No Hit
GTATAAGGGAATGTGGTGCTTATGGCATTTTGCGCTGGAATTTTTAGGAA68190.11281888209872572No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT68160.1127692477467245No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG67940.1124052624987157No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA67000.11085005280267815No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT66270.10964228357064895No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG65590.10851723825862178No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACG65450.10828561128261617No Hit
AGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTC64920.107408737730595No Hit
GCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA64200.10621751328256622No Hit
GTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTG63880.10568808019455343No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT63400.10489393056253424No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC62070.10269347429048108No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG61970.10252802645047707No Hit
CGATTGGACCAGGCGATCATGGAAAAGAACATAGTACTGAAAGCGAACTT61710.10209786206646669No Hit
GTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAA61300.10141952592245031No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)