FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A9a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A9a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1652321
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAA194141.174953292973944No Hit
GGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCA188101.1383986525620626No Hit
CAGTATGGCTGACTAAAACCACAGAAGTCTTACCTTTTCATCTTTGATTT164150.9934510303990568No Hit
ATGTAGCAGAGGACAAATAAGGGAAAATCAAAGATGAAAAGGTAAGACTT146190.8847554440087609No Hit
ATCTAGATATTAATCATTATTTCTTCCATTTGGTCCTCAGTTTGTCGTCT116440.7047056837018957No Hit
TAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAG104630.6332304679296578No Hit
CCCCAAGATTAATCCATTTTAGGCATCCTAGTTTGAGAAAAACATGCACA100400.607630115455774No Hit
AGTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCAAAAGGACTCAA99190.600307083187831No Hit
TTCTCTCTCTCTGTCAAATAAAATCTTGAAATAATGAATCTTTTGTTCTT95130.5757355864871293No Hit
GCCATACTGTGAGGAAAGAAGAAAGAACAAAAGATTCATTATTTCAAGAT87750.5310711417454599No Hit
CTGCTACATCTTGCCAGTCATTGAGTCCTTTTGATTCAACCTCTTAAGTG86040.5207220630858047No Hit
CTTTCTCTCTCTCTGTCAAATAAAATCTTGAAATAATGAATCTTTTGTTC82070.49669525473561127No Hit
TATTTGTCCTCTGCTACATCTTGCCAGTCATTGAGTCCTTTTGATTCAAC81370.49245878978721447No Hit
GTATGGCTGACTAAAACCACAGAAGTCTTACCTTTTCATCTTTGATTTTC81000.49021951545734754No Hit
ATTTTAGGCATCCTAGTTTGAGAAAAACATGCACAACTGAATTTGAAGAC80090.4847121110244317No Hit
GTTTTATTTTTATGGTGCCTTAGTACTTTTGAATTGGTTTTTGTGAATGC79860.4833201296842442No Hit
GTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAG78340.47412094865343957No Hit
ATCTTGGGGTGCCTAGGTGGCTTGGTCGGTTAGTGTCTGCCTTTGGCTCA75000.4539069587568033No Hit
TATCTAGATAAAGCATTCACAAAAACCAATTCAAAAGTACTAAGGCACCA74380.4501546612310804No Hit
ATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAA71110.4303643178292838No Hit
GCTTTATCTAGATATTAATCATTATTTCTTCCATTTGGTCCTCAGTTTGT70230.4250384761798706No Hit
AGATAAAGCATTCACAAAAACCAATTCAAAAGTACTAAGGCACCATAAAA69980.42352545298401456No Hit
GATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTA63330.38327903597424473No Hit
CTCTTTCTCTCTCTCTGTCAAATAAAATCTTGAAATAATGAATCTTTTGT60840.3682093249435189No Hit
CTTAAGAGGTTGAATCAAAAGGACTCAATGACTGGCAAGATGTAGCAGAG60030.36330712978894536No Hit
GTCAAATAAAATCTTGAAATAATGAATCTTTTGTTCTTTCTTCTTTCCTC59060.35743659978902403No Hit
CAGTAACTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAA58490.35398690690247236No Hit
GGACAAATAAGGGAAAATCAAAGATGAAAAGGTAAGACTTCTGTGGTTTT58020.35114242329426304No Hit
ATATTAATCATTATTTCTTCCATTTGGTCCTCAGTTTGTCGTCTTCAAAT57720.34932679545923584No Hit
AGTCTTACCTTTTCATCTTTGATTTTCCCTTATTTGTCCTCTGCTACATC57320.3469059583458662No Hit
GACTAAAACCACAGAAGTCTTACCTTTTCATCTTTGATTTTCCCTTATTT55180.33395447978933873No Hit
CTTTTGTTCTTTCTTCTTTCCTCACAGTATGGCTGACTAAAACCACAGAA51280.31035131793398496No Hit
GTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAGGTT49650.30048640669700377No Hit
GTACTAAGGCACCATAAAAATAAAACAGATAAAATTGGTTTTAAAAATAG45800.2771858494808212No Hit
ATTATATTCCTGGCTTTAAAGTTGAGAGAGTTGCCTGGGAATACTTTCGA45430.27494657515095433No Hit
GCCTTAGTACTTTTGAATTGGTTTTTGTGAATGCTTTATCTAGATATTAA44650.27022594277988354No Hit
TCATTATTTCTTCCATTTGGTCCTCAGTTTGTCGTCTTCAAATTCAGTTG44430.2688944823675303No Hit
GCCTAAAATGGATTAATCTTGGGGTGCCTAGGTGGCTTGGTCGGTTAGTG43700.26447645463563074No Hit
CTCTCTGTCAAATAAAATCTTGAAATAATGAATCTTTTGTTCTTTCTTCT43090.2607846780377421No Hit
AGCAGGGAGAGAGCAGCAGGCAGCAGGATAAGTAGGCTTCCCACTGAGCA42910.25969530133672575No Hit
GGTAAGACTTCTGTGGTTTTAGTCAGCCATACTGTGAGGAAAGAAGAAAG42000.25418789690380983No Hit
CTCTCTCTCTGTCAAATAAAATCTTGAAATAATGAATCTTTTGTTCTTTC41420.2506776830894239No Hit
AGGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGA40740.24656225999669557No Hit
GCACTTAAGAGGTTGAATCAAAAGGACTCAATGACTGGCAAGATGTAGCA40040.24232579504829874No Hit
AGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTT39830.24105485556377967No Hit
TCTTTCTTCTTTCCTCACAGTATGGCTGACTAAAACCACAGAAGTCTTAC38820.23494224185252138No Hit
ATTTTTATGGTGCCTTAGTACTTTTGAATTGGTTTTTGTGAATGCTTTAT38290.23173463267730665No Hit
GCCTGGGTGTGTCCACTGGCTAAGTGTCTGCCTTCAGCTCAGTTCCTGAC38060.23034265133711915No Hit
GATTAATATCTAGATAAAGCATTCACAAAAACCAATTCAAAAGTACTAAG37750.22846650257425768No Hit
GTCATTGAGTCCTTTTGATTCAACCTCTTAAGTGCTTTTTGCATTCTATT37430.22652983288356196No Hit
GATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCAA37250.22544045618254566No Hit
GATTAATCCATTTTAGGCATCCTAGTTTGAGAAAAACATGCACAACTGAA36420.22041721917230367No Hit
CTTAAGTGCTTTTTGCATTCTATTCACTTTCTCTAATCCTACTACCACTA36170.21890419597644767No Hit
CCTTAGTACTTTTGAATTGGTTTTTGTGAATGCTTTATCTAGATATTAAT35710.21612023329607263No Hit
GTGGAGAGCCTCCTTCTCCCTCTTCTGTTTCCCCTGCTTGTGCGCTCTTT34260.20734469876010775No Hit
GAGAGAAAGAGCGCACAAGCAGGGGAAACAGAAGAGGGAGAAGGAGGCTC34060.20613428020342292No Hit
TCCTGGGATTGAGCCCCACATTGAGCTCCCTGCTCAGTGGAGAGCCTCCT33950.20546854999724629No Hit
GAGGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTG33850.20486334071890389No Hit
TTTTTATGGTGCCTTAGTACTTTTGAATTGGTTTTTGTGAATGCTTTATC33830.20474229886323542No Hit
CTTTTGATTCAACCTCTTAAGTGCTTTTTGCATTCTATTCACTTTCTCTA33130.20050583391483856No Hit
GTCTTACCTTTTCATCTTTGATTTTCCCTTATTTGTCCTCTGCTACATCT32540.19693509917261842No Hit
TAACAGGGATGCCTGGGTGTGTCCACTGGCTAAGTGTCTGCCTTCAGCTC32280.19536155504892816No Hit
AATCAAAAGGACTCAATGACTGGCAAGATGTAGCAGAGGACAAATAAGGG32050.1939695737087406No Hit
CAGCCATACTGTGAGGAAAGAAGAAAGAACAAAAGATTCATTATTTCAAG32050.1939695737087406No Hit
GATGTAGCAGAGGACAAATAAGGGAAAATCAAAGATGAAAAGGTAAGACT31950.1933643644303982No Hit
ATCCCAGGAAGGACCCTGGGATCATGACCTGAGCCAAAGGCAGACACTAA31810.19251707144071886No Hit
CTACTGAAGAAACAACAGGGGAGAGGTAACAGTAACTATAATAAGATAGT31750.1921539458737134No Hit
GGATAAGTAGGCTTCCCACTGAGCAGGGAGCTCCATGCAGTGCTCAATCC31580.1911250901005313No Hit
GCTAATAACAGGGATGCCTGGGTGTGTCCACTGGCTAAGTGTCTGCCTTC31560.19100404824486283No Hit
ATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCA31320.18955154597684107No Hit
GTTTTAGTCAGCCATACTGTGAGGAAAGAAGAAAGAACAAAAGATTCATT30470.18440726711093064No Hit
ATCTTGAAATAATGAATCTTTTGTTCTTTCTTCTTTCCTCACAGTATGGC30280.18325736948208007No Hit
GCTCAGTGGAGAGCCTCCTTCTCCCTCTTCTGTTTCCCCTGCTTGTGCGC30050.18186538814189251No Hit
CACCCAGGCATCCCTGTTATTAGCTATTTTTAAAACCAATTTTATCTGTT29880.18083653236871045No Hit
ATCATGACCTGAGCCAAAGGCAGACACTAACCGACCAAGCCACCTAGGCA29830.18053392772953925No Hit
GAGAGAGAAAGAGCGCACAAGCAGGGGAAACAGAAGAGGGAGAAGGAGGC29530.17871829989451202No Hit
AGATGTAGCAGAGGACAAATAAGGGAAAATCAAAGATGAAAAGGTAAGAC29390.17787100690483265No Hit
CCTCACAGTATGGCTGACTAAAACCACAGAAGTCTTACCTTTTCATCTTT29240.17696319298731905No Hit
CAACAGGGGAGAGGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATT29180.1766000674203136No Hit
GCTACATCTTGCCAGTCATTGAGTCCTTTTGATTCAACCTCTTAAGTGCT28580.1729688117502592No Hit
CACTTAGCCAGTGGACACACCCAGGCATCCCTGTTATTAGCTATTTTTAA27990.169398077008039No Hit
CCACTGAGCAGGGAGCTCAATGTGGGGCTCAATCCCAGGAAGGACCCTGG27790.1681876584513542No Hit
ATCCCAGGACGCAGGGGTCAGGAACTGAGCTGAAGGCAGACACTTAGCCA27350.1655247376266476No Hit
GGTCATGATCCCAGGGTCCTTCCTGGGATTGAGCCCCACATTGAGCTCCC27270.1650405702039737No Hit
ATCCTAGTTTGAGAAAAACATGCACAACTGAATTTGAAGACGACAAACTG27110.16407223535862583No Hit
GTCCTCAGTTTGTCGTCTTCAAATTCAGTTGTGCATGTTTTTCTCAAACT26260.1589279564927154No Hit
GAGAAAGAGCGCACAAGCAGGGGAAACAGAAGAGGGAGAAGGAGGCTCTC26060.15771753793603058No Hit
AATGAATCTTTTGTTCTTTCTTCTTTCCTCACAGTATGGCTGACTAAAAC26040.1575964960803621No Hit
GAGCAGGGAGCTCCATGCAGTGCTCAATCCCAGGACGCAGGGGTCAGGAA25900.15674920309068274No Hit
GGCTAAGTGTCTGCCTTCAGCTCAGTTCCTGACCCCTGCGTCCTGGGATT25540.15457044968865008No Hit
GTTAGTGTCTGCCTTTGGCTCAGGTCATGATCCCAGGGTCCTTCCTGGGA25340.15336003113196528No Hit
TCACAGTATGGCTGACTAAAACCACAGAAGTCTTACCTTTTCATCTTTGA25300.15311794742062831No Hit
ACATTGAGCTCCCTGCTCAGTGGAGAGCCTCCTTCTCCCTCTTCTGTTTC25170.15233117535878318No Hit
CTTCTTTCCTCACAGTATGGCTGACTAAAACCACAGAAGTCTTACCTTTT25090.15184700793610925No Hit
CCCTTATTTGTCCTCTGCTACATCTTGCCAGTCATTGAGTCCTTTTGATT25060.15166544515260655No Hit
CCTCTTAAGTGCTTTTTGCATTCTATTCACTTTCTCTAATCCTACTACCA24760.14984981731757935No Hit
CTATTCACTTTCTCTAATCCTACTACCACTATCTTATTATAGTTACTGTT24720.14960773360624238No Hit
GGGGAGAGGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATTAGAGA24510.14833679412172332No Hit
GAGCAGGGAGCTCAATGTGGGGCTCAATCCCAGGAAGGACCCTGGGATCA24090.14579491515268522No Hit
TTATTTGACAGAGAGAGAGAAAGAGCGCACAAGCAGGGGAAACAGAAGAG24060.1456133523691825No Hit
GGTCAGGAACTGAGCTGAAGGCAGACACTTAGCCAGTGGACACACCCAGG24050.14555283144134826No Hit
CTCAATGACTGGCAAGATGTAGCAGAGGACAAATAAGGGAAAATCAAAGA23770.14385824546198953No Hit
ACTGAAGAAACAACAGGGGAGAGGTAACAGTAACTATAATAAGATAGTGG23630.14301095247231016No Hit
ACAGAAGTCTTACCTTTTCATCTTTGATTTTCCCTTATTTGTCCTCTGCT23580.14270834783313896No Hit
TTATTTCTTCCATTTGGTCCTCAGTTTGTCGTCTTCAAATTCAGTTGTGC23420.1417400129877911No Hit
CTGTCAAATAAAATCTTGAAATAATGAATCTTTTGTTCTTTCTTCTTTCC23120.1399243851527639No Hit
AGATATTAATCATTATTTCTTCCATTTGGTCCTCAGTTTGTCGTCTTCAA22820.1381087573177367No Hit
ATAATGAATCTTTTGTTCTTTCTTCTTTCCTCACAGTATGGCTGACTAAA22660.13714042247238883No Hit
GGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAA22090.13369072958583714No Hit
CATTGAGTCCTTTTGATTCAACCTCTTAAGTGCTTTTTGCATTCTATTCA21910.1326013528848208No Hit
CTTCTGTTTCCCCTGCTTGTGCGCTCTTTCTCTCTCTCTGTCAAATAAAA21680.13120937154463327No Hit
AGAAACAACAGGGGAGAGGTAACAGTAACTATAATAAGATAGTGGTAGTA21660.1310883296889648No Hit
ACAGGGGAGAGGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATTAG21510.13018051577145118No Hit
GCCTAGGTGGCTTGGTCGGTTAGTGTCTGCCTTTGGCTCAGGTCATGATC21390.1294542646374403No Hit
CCACAGAAGTCTTACCTTTTCATCTTTGATTTTCCCTTATTTGTCCTCTG21360.12927270185393758No Hit
CAAATAAGGGAAAATCAAAGATGAAAAGGTAAGACTTCTGTGGTTTTAGT21330.12909113907043487No Hit
GTTATTAGCTATTTTTAAAACCAATTTTATCTGTTTTATTTTTATGGTGC21330.12909113907043487No Hit
ACTGTGAGGAAAGAAGAAAGAACAAAAGATTCATTATTTCAAGATTTTAT21310.12897009721476638No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC21020.1272149903075734No Hit
GAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCAAAAGGA20850.12618613453439131No Hit
TAATGAATCTTTTGTTCTTTCTTCTTTCCTCACAGTATGGCTGACTAAAA20780.12576248803955165No Hit
AGCGAAAGCAGGCTACTGAAGAAACAACAGGGGAGAGGTAACAGTAACTA20730.12545988340038045No Hit
CAGTTTGTCGTCTTCAAATTCAGTTGTGCATGTTTTTCTCAAACTAGGAT20670.125096757833375No Hit
GCTCCATGCAGTGCTCAATCCCAGGACGCAGGGGTCAGGAACTGAGCTGA20550.1243705066993641No Hit
GGATTAATCTTGGGGTGCCTAGGTGGCTTGGTCGGTTAGTGTCTGCCTTT20490.12400738113235867No Hit
TCCCTGTTATTAGCTATTTTTAAAACCAATTTTATCTGTTTTATTTTTAT20440.12370477649318745No Hit
GGCAAGATGTAGCAGAGGACAAATAAGGGAAAATCAAAGATGAAAAGGTA20200.12225227422516569No Hit
GGAGAGAGCAGCAGGCAGCAGGATAAGTAGGCTTCCCACTGAGCAGGGAG19870.12025508360663575No Hit
AAATAAAACAGATAAAATTGGTTTTAAAAATAGCTAATAACAGGGATGCC19500.11801580927676887No Hit
GTGTCTGCCTTTGGCTCAGGTCATGATCCCAGGGTCCTTCCTGGGATTGA19390.11735007907059222No Hit
GGCTACTGAAGAAACAACAGGGGAGAGGTAACAGTAACTATAATAAGATA19260.11656330700874709No Hit
GGTTTTTGTGAATGCTTTATCTAGATATTAATCATTATTTCTTCCATTTG19240.11644226515307862No Hit
GAACAAAAGATTCATTATTTCAAGATTTTATTTGACAGAGAGAGAGAAAG19160.11595809773040468No Hit
CCTTTTCATCTTTGATTTTCCCTTATTTGTCCTCTGCTACATCTTGCCAG19150.11589757680257044No Hit
AAAGCAGGCTACTGAAGAAACAACAGGGGAGAGGTAACAGTAACTATAAT18970.11480820010155411No Hit
GCTCAATGTGGGGCTCAATCCCAGGAAGGACCCTGGGATCATGACCTGAG18880.11426351175104596No Hit
CACCTAGGCACCCCAAGATTAATCCATTTTAGGCATCCTAGTTTGAGAAA18630.11275048855518995No Hit
AACTAGGATGCCTAAAATGGATTAATCTTGGGGTGCCTAGGTGGCTTGGT18560.11232684206035025No Hit
AACATGCACAACTGAATTTGAAGACGACAAACTGAGGACCAAATGGAAGA18500.11196371649334481No Hit
GGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAGGT18040.10917975381296975No Hit
CCCTGGGATCATGACCTGAGCCAAAGGCAGACACTAACCGACCAAGCCAC17900.10833246082329039No Hit
GACACTAACCGACCAAGCCACCTAGGCACCCCAAGATTAATCCATTTTAG17890.10827193989545614No Hit
GGGGAAACAGAAGAGGGAGAAGGAGGCTCTCCACTGAGCAGGGAGCTCAA17860.10809037711195343No Hit
AACTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCA17800.10772725154494797No Hit
GTGAGGAAAGAAGAAAGAACAAAAGATTCATTATTTCAAGATTTTATTTG17780.1076062096892795No Hit
ATCCCAGGGTCCTTCCTGGGATTGAGCCCCACATTGAGCTCCCTGCTCAG17770.10754568876144527No Hit
GTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCAAAAGGACTCAAT17370.10512485164807564No Hit
CCTAGGCACCCCAAGATTAATCCATTTTAGGCATCCTAGTTTGAGAAAAA17300.10470120515323597No Hit
GCAAAAAGCACTTAAGAGGTTGAATCAAAAGGACTCAATGACTGGCAAGA17300.10470120515323597No Hit
AATAAGGGAAAATCAAAGATGAAAAGGTAAGACTTCTGTGGTTTTAGTCA17260.104459121441899No Hit
GAAGAAACAACAGGGGAGAGGTAACAGTAACTATAATAAGATAGTGGTAG17220.10421703773056204No Hit
AAAGTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCAAAAGGACTC17200.10409599587489357No Hit
ATGTGGGGCTCAATCCCAGGAAGGACCCTGGGATCATGACCTGAGCCAAA17190.10403547494705932No Hit
GATGAAAAGGTAAGACTTCTGTGGTTTTAGTCAGCCATACTGTGAGGAAA17180.10397495401922507No Hit
GTTCCTGACCCCTGCGTCCTGGGATTGAGCACTGCATGGAGCTCCCTGCT17150.10379339123572236No Hit
GGCAGACACTAACCGACCAAGCCACCTAGGCACCCCAAGATTAATCCATT17090.10343026566871692No Hit
GCTCTCCACTGAGCAGGGAGCTCAATGTGGGGCTCAATCCCAGGAAGGAC17000.10288557731820876No Hit
GAAGAAAGAACAAAAGATTCATTATTTCAAGATTTTATTTGACAGAGAGA16970.10270401453470603No Hit
TCTTTCCTCACAGTATGGCTGACTAAAACCACAGAAGTCTTACCTTTTCA16800.10167515876152394No Hit
GTCCTTTTGATTCAACCTCTTAAGTGCTTTTTGCATTCTATTCACTTTCT16720.10119099133885001No Hit
CCATAAAAATAAAACAGATAAAATTGGTTTTAAAAATAGCTAATAACAGG16670.10088838669967881No Hit
AAAGCAGGGAGAGAGCAGCAGGCAGCAGGATAAGTAGGCTTCCCACTGAG16670.10088838669967881No Hit
GAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCAAAAGGACTCAATGA16640.1007068239161761No Hit
ATACTGTGAGGAAAGAAGAAAGAACAAAAGATTCATTATTTCAAGATTTT16600.10046474020483913No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)