FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4098701
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAAAGCGCGCGCTATGACGGGCTACCTCCCACTAGGCCAGGACTATT299630.7310364918055745No Hit
CTTTAAGGAGCGCCTGAAATTCCCACGTCTCCGCCCACCGCTTGTAGTAG261380.6377142416585156No Hit
ACCATGGACCAATTGCAAGAGCTGGACCGCAAATTCGATATCGCGCAGGC228730.5580548568924643No Hit
ACCTACCGCACTTTGATGATGCGCATTGCCGCCTGCGATGTGGCGGGCAC200790.48988691783079563No Hit
ACTACAAGCGGTGGGCGGAGACGTGGGAATTTCAGGCGCTCCTTAAAGCG174810.4265009816524797No Hit
GTAGTAGGCCACGTGGGATTCCAAGGTGCGCACCAATGCGCCCGACTTTC174290.4252322870099575No Hit
GTCCTGGCCTAGTGGGAGGTAGCCCGTCATAGCGCGCGCTTTAAGGAGCG137570.3356429268687811No Hit
CTCATAGGCTTCAATCAGCTGGGTGCCGTCCTCGCGGCCGACGTACCCCG126770.30929311506255275No Hit
GCCTACTACAAGCGGTGGGCGGAGACGTGGGAATTTCAGGCGCTCCTTAA123780.3019981208680506No Hit
GTGCTTGAGCTCTGCGGGAACATTGGCCAGCACCCGGCGGCGCATCGCCT123170.3005098444604766No Hit
CCTTGGAATCCCACGTGGCCTACTACAAGCGGTGGGCGGAGACGTGGGAA121880.297362505828066No Hit
GTACAGGCGATGCGCCGCCGGGTGCTGGCCAATGTTCCCGCAGAGCTCAA121620.29672815850680495No Hit
ATTCCAAGGTGCGCACCAATGCGCCCGACTTTCCCTCGGGGCGCAGGTTC120480.2939467894828142No Hit
ATATAGTTCAGCTCGCCTGCGCCGCACTTGCCCATCGCCATGACTGCCAG118230.2884572453565166No Hit
GTCATAGCGCGCGCTTTAAGGAGCGCCTGAAATTCCCACGTCTCCGCCCA117020.28550509051526324No Hit
GCTTTAGCTCGCGGTGCTTGAGCTCTGCGGGAACATTGGCCAGCACCCGG107430.26210743355028826No Hit
AAATAGTCCTGGCCTAGTGGGAGGTAGCCCGTCATAGCGCGCGCTTTAAG105370.2570814509279892No Hit
CGCCTAGCTTTAGCTCGCGGTGCTTGAGCTCTGCGGGAACATTGGCCAGC105220.2567154813195693No Hit
GGCCAGGACTATTTGGACCAGATTCGCCCGATGGTGTGGACGGCATCGCA104890.2559103481810457No Hit
GTACATCCTCAACAAAAGACTCGCGCTGCGATGCCGTCCACACCATCGGG99060.24168632940046128No Hit
CGCCTGTACATCCTCAACAAAAGACTCGCGCTGCGATGCCGTCCACACCA95810.23375698788469812No Hit
GCGTCTCATCAGAGCGACCATGGACCAATTGCAAGAGCTGGACCGCAAAT92680.22612042205567082No Hit
ATTTGGACCAGATTCGCCCGATGGTGTGGACGGCATCGCAGCGCGAGTCT90800.22153360296347552No Hit
GTTTAATCGCATTGGATCCACCACCTTCTTCGAGGTGGATGCGAACCTGC90660.22119203132895032No Hit
CATCAGAGCGACCATGGACCAATTGCAAGAGCTGGACCGCAAATTCGATA90140.21992333668642822No Hit
ATTCGATATCGCGCAGGCCGCCCACGCCTAGCTTTAGCTCGCGGTGCTTG89640.21870343799169542No Hit
GTCCATGGTCGCTCTGATGAGACGCTGCGCGCGCTTTCCACGGTGGAGGC87680.21392143510834288No Hit
CGTAAGGGCCAGACCAAGCTGCAGCCGGAGGTAGGATTTCGCCAGATTAC87290.21296991412645128No Hit
CCTGTACATCCTCAACAAAAGACTCGCGCTGCGATGCCGTCCACACCATC86900.21201839314455972No Hit
GGTGTGGACGGCATCGCAGCGCGAGTCTTTTGTTGAGGATGTACAGGCGA85260.20801712542583614No Hit
GAGCTGGACCGCAAATTCGATATCGCGCAGGCCGCCCACGCCTAGCTTTA83120.20279595901237976No Hit
GTCCACACCATCGGGCGAATCTGGTCCAAATAGTCCTGGCCTAGTGGGAG82190.2005269474401768No Hit
GCTTGTAGTAGGCCACGTGGGATTCCAAGGTGCGCACCAATGCGCCCGAC78850.1923780241593617No Hit
TCTTCGAGGTGGATGCGAACCTGCGCCCCGAGGGAAAGTCGGGCGCATTG78150.1906701659867358No Hit
CTGCGGGAACATTGGCCAGCACCCGGCGGCGCATCGCCTGTACATCCTCA76660.1870348678764321No Hit
GCTGGGTGCCGTCCTCGCGGCCGACGTACCCCGCTGAAATCAGCGCCTCC76620.18693727598085344No Hit
TCAATCAGCTGGGTGCCGTCCTCGCGGCCGACGTACCCCGCTGAAATCAG73720.17986186355140324No Hit
GGACTATTTGGACCAGATTCGCCCGATGGTGTGGACGGCATCGCAGCGCG73450.17920311825624752No Hit
GAAATATAGTTCAGCTCGCCTGCGCCGCACTTGCCCATCGCCATGACTGC73330.17891034256951166No Hit
CTTTGATGATGCGCATTGCCGCCTGCGATGTGGCGGGCACCTTCCATTCA72090.17588499380657432No Hit
GCGCAGGCAAGGGAGGCGGTTAAGGCGGCGTCGGCAAGCGCGGTGGTCAG70300.17151775647943093No Hit
AACTCATAGGCTTCAATCAGCTGGGTGCCGTCCTCGCGGCCGACGTACCC68870.16802884621249511No Hit
ATGTACAGGCGATGCGCCGCCGGGTGCTGGCCAATGTTCCCGCAGAGCTC68740.1677116725518646No Hit
GCGCACGACCTACCGCACTTTGATGATGCGCATTGCCGCCTGCGATGTGG68660.16751648876070735No Hit
GTGCGCACCAATGCGCCCGACTTTCCCTCGGGGCGCAGGTTCGCATCCAC68590.16734570294344475No Hit
ACCGTGGAAAGCGCGCGCAGCGTCTCATCAGAGCGACCATGGACCAATTG68350.166760151569973No Hit
CTACAAGCGGTGGGCGGAGACGTGGGAATTTCAGGCGCTCCTTAAAGCGC68090.16612580424871196No Hit
GGTCCAAATAGTCCTGGCCTAGTGGGAGGTAGCCCGTCATAGCGCGCGCT67420.16449113999777001No Hit
CGCTATGACGGGCTACCTCCCACTAGGCCAGGACTATTTGGACCAGATTC65090.1588064120803152No Hit
CCTTCCATTCACGTAAGGGCCAGACCAAGCTGCAGCCGGAGGTAGGATTT64550.15748892149000376No Hit
ATCCAATGCGATTAAACTCCGCCGCCAGCCGCGTGGCTCGAGGGGTTGCC64320.1569277680904267No Hit
ACCTCCCACTAGGCCAGGACTATTTGGACCAGATTCGCCCGATGGTGTGG63650.15529310383948475No Hit
CACTAGGCCAGGACTATTTGGACCAGATTCGCCCGATGGTGTGGACGGCA63540.15502472612664353No Hit
AGCTAGGCGTGGGCGGCCTGCGCGATATCGAATTTGCGGTCCAGCTCTTG63450.15480514436159162No Hit
GGTGCGCACCTTGGAATCCCACGTGGCCTACTACAAGCGGTGGGCGGAGA62810.15324367403233366No Hit
CTATGACGGGCTACCTCCCACTAGGCCAGGACTATTTGGACCAGATTCGC61900.15102345840791997No Hit
ATATCGAATTTGCGGTCCAGCTCTTGCAATTGGTCCATGGTCGCTCTGAT61830.15085267259065738No Hit
TCCAAGGGGTCATCGCCGTAAATGGTGCGCACGGCGCAGGCAAGGGAGGC61670.15046230500834287No Hit
GGCCTAGTGGGAGGTAGCCCGTCATAGCGCGCGCTTTAAGGAGCGCCTGA61430.14987675363487116No Hit
GTGTGGACGGCATCGCAGCGCGAGTCTTTTGTTGAGGATGTACAGGCGAT59070.14411883179573237No Hit
ACATTGGCCAGCACCCGGCGGCGCATCGCCTGTACATCCTCAACAAAAGA59010.14397244395236442No Hit
GCTCAAGCACCGCGAGCTAAAGCTAGGCGTGGGCGGCCTGCGCGATATCG58560.1428745351271049No Hit
GGTTCGCATCCACCTCGAAGAAGGTGGTGGATCCAATGCGATTAAACTCC57910.14128866682395227No Hit
CCTCGAAGAAGGTGGTGGATCCAATGCGATTAAACTCCGCCGCCAGCCGC57770.1409470951894271No Hit
CGATTAAACTCCGCCGCCAGCCGCGTGGCTCGAGGGGTTGCCTCGCTGCC57750.14089829924163777No Hit
GGCTTCAATCAGCTGGGTGCCGTCCTCGCGGCCGACGTACCCCGCTGAAA57650.14065431950269122No Hit
TGCCTGCGCCGTGCGCACCATTTACGGCGATGACCCCTTGGATGCACAGC57590.1405079316593233No Hit
AGTGGGAGGTAGCCCGTCATAGCGCGCGCTTTAAGGAGCGCCTGAAATTC57560.14043473773763931No Hit
GACGTACCCCGCTGAAATCAGCGCCTCCAGCGCCTCCACCGTGGAAAGCG57560.14043473773763931No Hit
CGCTTTAAGGAGCGCCTGAAATTCCCACGTCTCCGCCCACCGCTTGTAGT57360.1399467782597462No Hit
GGCCAGCACCCGGCGGCGCATCGCCTGTACATCCTCAACAAAAGACTCGC56060.13677504165344093No Hit
ATGCGAACCTGCGCCCCGAGGGAAAGTCGGGCGCATTGGTGCGCACCTTG55880.1363358781233371No Hit
CTGCGATGCCGTCCACACCATCGGGCGAATCTGGTCCAAATAGTCCTGGC54590.13318853949092652No Hit
AAACAAGGAAGTGATGGGACCGCAGAGATGAGACCCCCAGGGATGAGATG54050.1318710489006151No Hit
GTGGGAGGTAGCCCGTCATAGCGCGCGCTTTAAGGAGCGCCTGAAATTCC53170.12972402719788537No Hit
GGATCCACCACCTTCTTCGAGGTGGATGCGAACCTGCGCCCCGAGGGAAA53070.12948004745893882No Hit
CCATCGGGCGAATCTGGTCCAAATAGTCCTGGCCTAGTGGGAGGTAGCCC52900.12906528190272967No Hit
AGCTAAAGCTAGGCGTGGGCGGCCTGCGCGATATCGAATTTGCGGTCCAG52610.12835774065978464No Hit
GCTTGAGCTCTGCGGGAACATTGGCCAGCACCCGGCGGCGCATCGCCTGT52580.1282845467381007No Hit
ATCCACCACCTTCTTCGAGGTGGATGCGAACCTGCGCCCCGAGGGAAAGT51510.1256739635313725No Hit
ACAAAAGACTCGCGCTGCGATGCCGTCCACACCATCGGGCGAATCTGGTC51410.12542998379242595No Hit
GTGGTGGATCCAATGCGATTAAACTCCGCCGCCAGCCGCGTGGCTCGAGG51300.1251616060795847No Hit
CCGTAAATGGTGCGCACGGCGCAGGCAAGGGAGGCGGTTAAGGCGGCGTC51060.12457605470611299No Hit
TCTCCGCCCACCGCTTGTAGTAGGCCACGTGGGATTCCAAGGTGCGCACC50970.12435647294106109No Hit
TCTCATCAGAGCGACCATGGACCAATTGCAAGAGCTGGACCGCAAATTCG50910.12421008509769314No Hit
CCCTCGAGCCACGCGGCTGGCGGCGGAGTTTAATCGCATTGGATCCACCA50220.12252662489896189No Hit
ATTAAACTCCGCCGCCAGCCGCGTGGCTCGAGGGGTTGCCTCGCTGCCGA49250.12016002143118026No Hit
GGCCAGACCAAGCTGCAGCCGGAGGTAGGATTTCGCCAGATTACGGCGCT49180.11998923561391767No Hit
CGCCTCCACCGTGGAAAGCGCGCGCAGCGTCTCATCAGAGCGACCATGGA48220.11764703012003071No Hit
TTGATGATGCGCATTGCCGCCTGCGATGTGGCGGGCACCTTCCATTCACG48130.1174274483549788No Hit
GCATCGCAGCGCGAGTCTTTTGTTGAGGATGTACAGGCGATGCGCCGCCG47530.11596356992129946No Hit
CATTGGATCCACCACCTTCTTCGAGGTGGATGCGAACCTGCGCCCCGAGG47400.11564639626066894No Hit
ATGGTGTGGACGGCATCGCAGCGCGAGTCTTTTGTTGAGGATGTACAGGC47250.11528042665224908No Hit
GTTCCCGCAGAGCTCAAGCACCGCGAGCTAAAGCTAGGCGTGGGCGGCCT47060.11481686514825064No Hit
GCTCGAGGGGTTGCCTCGCTGCCGACGAAAATGACGTCCACATCGGAAAT46210.11274303736720488No Hit
CAACAAAAGACTCGCGCTGCGATGCCGTCCACACCATCGGGCGAATCTGG45700.11149874069857743No Hit
AGCGAAAGCAGGCGCAGAAACTCATAGGCTTCAATCAGCTGGGTGCCGTC45550.1111327710901576No Hit
GTGGAAAGCGCGCGCAGCGTCTCATCAGAGCGACCATGGACCAATTGCAA45330.11059601566447516No Hit
GCATTGGATCCACCACCTTCTTCGAGGTGGATGCGAACCTGCGCCCCGAG44200.10783904461437903No Hit
GCTTCAATCAGCTGGGTGCCGTCCTCGCGGCCGACGTACCCCGCTGAAAT44060.10749747297985388No Hit
GACCAATTGCAAGAGCTGGACCGCAAATTCGATATCGCGCAGGCCGCCCA43880.10705830944975005No Hit
CTCCTGGGCCCTGGACCCTGGGCCCTGCCTGGGGGTCCCATCTCATCCCT43290.10561882898996536No Hit
CCGCTTGTAGTAGGCCACGTGGGATTCCAAGGTGCGCACCAATGCGCCCG43290.10561882898996536No Hit
CCCCTCGAGCCACGCGGCTGGCGGCGGAGTTTAATCGCATTGGATCCACC43240.10549683912049207No Hit
TTGGAATCCCACGTGGCCTACTACAAGCGGTGGGCGGAGACGTGGGAATT43020.10496008369480965No Hit
CTTCGAGGTGGATGCGAACCTGCGCCCCGAGGGAAAGTCGGGCGCATTGG42660.10408175663460204No Hit
GGATTCCAAGGTGCGCACCAATGCGCCCGACTTTCCCTCGGGGCGCAGGT42480.10364259310449823No Hit
CCAATGCGCCCGACTTTCCCTCGGGGCGCAGGTTCGCATCCACCTCGAAG42360.10334981741776236No Hit
GCTGAAATCAGCGCCTCCAGCGCCTCCACCGTGGAAAGCGCGCGCAGCGT42220.10300824578323717No Hit
GACCAAGCTGCAGCCGGAGGTAGGATTTCGCCAGATTACGGCGCTGACCA41230.10059284636766624No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)