FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A8a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A8a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1228056
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAGTTGTTGAACATGGATGAATGAAACCTGTCGAGTGAGAGTGGAAT84350.6868579283029438No Hit
GTTCACAGCTGCACAGGCTCTGTGACAACAAACATTTGGTGAATTGAACT71520.5823838652309016No Hit
CAACTACTCTGTGCCCGGGCAACCAGGATTGGGAACCCAGCTTTTTAGTT63170.5143902232471483No Hit
CCCTCAGGGAGGGACCTCAGCATTGCCTTCCCTCGGCTCCCAGCAGTATC50580.41187046844769293No Hit
CCCTGAGGGTGTGGGTGTCAGGTGGAGCCCACTGTTCACAGCTGCACAGG48890.3981088810282267No Hit
ACCTAGGTCTCTGCACTCCAGAGCATGGGCTGTGCCCAGCACATGCTGAG40940.33337241949878504No Hit
GCTGTGAACAGTGGGCTCCACCTGACACCCACACCCTCAGGGAGGGACCT39240.3195294025679611No Hit
CTCCAGAGCATGGGCTGTGCCCAGCACATGCTGAGACTCGGGGAGTGGGG39060.3180636713635209No Hit
GGCTAGGAACCTAGGTCTCTGCACTCCAGAGCATGGGCTGTGCCCAGCAC37880.3084549890233019No Hit
TCCTAGCGCAGCACTCCAGGATTCCCACTCAGCAGCAGGGCAGTGACCGA35910.29241337528581757No Hit
ATCCTGGACGGGAGCAAGTCTGTGGTGGAAGCGGAGATGGTGGTGAGACC35490.2889933358087905No Hit
GCCCTGGTGTGCATTGGTTCTCATGCAGATCTTCAGGCTGGGAGTAAAAG35270.28720188655891915No Hit
CTCCAGGATTCCCACTCAGCAGCAGGGCAGTGACCGACTTTTACTCCCAG34810.2834561290364609No Hit
GTGCCCGGGCAACCAGGATTGGGAACCCAGCTTTTTAGTTCAATTCACCA34380.27995466004807595No Hit
CCCTGGGGTTTTGAGCTTGGGAGACTGGAGAGATGAGGAATCCTGGACGG33110.2696131121056369No Hit
GCATAGCCCTGGTGTGCATTGGTTCTCATGCAGATCTTCAGGCTGGGAGT32540.26497162995824297No Hit
CCCCACAGATACTGCTGGGAGCCGAGGGAAGGCAATGCTGAGGTCCCTCC32430.26407590533330727No Hit
AATTCATTCCACTCTCACTCGACAGGTTTCATTCATCCATGTTCAACAAC31150.2536529278795104No Hit
GGATTGGGAACCCAGCTTTTTAGTTCAATTCACCAAATGTTTGTTGTCAC29390.23932133388053964No Hit
CACCAGGGCTATGCTGCCTCCGTGACCAAGTGGTCCCCAGGGCCTGGTCT29180.2376113141420261No Hit
GACATATGCCTTCCAGCATATTCTCGTCTCCAGCACACAAATTCATTCCA27900.22718833668822921No Hit
CTGTGGCATCACCCCACAGATACTGCTGGGAGCCGAGGGAAGGCAATGCT27700.22555974646107343No Hit
GGTTATTAGTAGAAACAAGGCGGGTTATTAGTAGAAACAAGGCGGGTTAT26820.21839394946158805No Hit
TTTTAGTTCAATTCACCAAATGTTTGTTGTCACAGAGCCTGTGCAGCTGT26560.21627678216628557No Hit
AGTAGAAACAAGGCGGGTTATTAGTAGAAACAAGGCGGGTTATTAGTAGA26170.21310103122333182No Hit
GTGGGGAGGCATCCATACTTTGGAGAAAGAGCAGAGCCCACTGTGGCATC26150.21293817220061623No Hit
CTCTGTGCCCGGGCAACCAGGATTGGGAACCCAGCTTTTTAGTTCAATTC26110.21261245415518512No Hit
CCAGGGGACTTGGCTGCAAACCCAACAGATGATGGAGAAGACATATGCCT26010.2117981590416072No Hit
TGCTGAGACTCGGGGAGTGGGGAGGCATCCATACTTTGGAGAAAGAGCAG25990.21163530001889166No Hit
GCCCAGCACATGCTGAGACTCGGGGAGTGGGGAGGCATCCATACTTTGGA25930.2111467229507449No Hit
TCCCAATCCTGGTTGCCCGGGCACAGAGTAGTTGTTGAACATGGATGAAT25910.21098386392802934No Hit
GACATATGCCTTCCGGCATATTCTCGTCTCCAGCACACAAATTCATTCCA25860.2105767163712404No Hit
CATCCATACTTTGGAGAAAGAGCAGAGCCCACTGTGGCATCACCCCACAG25780.20992528028037807No Hit
CTCCCACCTCTCCCCACCACCCTCCTAGCGCAGCACTCCAGGATTCCCAC25740.20959956223494694No Hit
TCTTCAGGCTGGGAGTAAAAGTCGGTCACTGCCCTGCTGCTGAGTGGGAA25200.20520236862162636No Hit
GGGCAGACTGAGCTCCAGGGGACTTGGCTGCAAACCCAACAGATGATGGA25060.2040623554626173No Hit
GGGCTATGCTGCCTCCGTGACCAAGTGGTCCCCAGGGCCTGGTCTCACCA24470.19925801429250783No Hit
GGCTCTGTGACAACAAACATTTGGTGAATTGAACTAAAAAGCTGGGTTCC23660.192662223872527No Hit
CTTTTACTCCCAGCCTGAAGATCTGCATGAGAACCAATGCACACCAGGGC23590.19209221729302248No Hit
GGTTTCATTCATCCATGTTCAACAACTACTCTGTGCCCGGGCAACCAGGA23430.19078934511129786No Hit
GCACAGGCTCTGTGACAACAAACATTTGGTGAATTGAACTAAAAAGCTGG22280.1814249513051522No Hit
CACAGAGCCTGTGCAGCTGTGAACAGTGGGCTCCACCTGACACCCACACC21840.17784205280540952No Hit
GAACTAAAAAGCTGGGTTCCCAATCCTGGTTGCCCGGGCACAGAGTAGTT21670.17645775111232714No Hit
AATGAAACCTGTCGAGTGAGAGTGGAATGAATTTGTGTGCTGGAGACGAG21350.1738520067488779No Hit
GAGCATGGGCTGTGCCCAGCACATGCTGAGACTCGGGGAGTGGGGAGGCA21280.1732820001693734No Hit
GATGAGGAATCCTGGACGGGAGCAAGTCTGTGGTGGAAGCGGAGATGGTG20490.16684906877210812No Hit
CAGCTGCACAGGCTCTGTGACAACAAACATTTGGTGAATTGAACTAAAAA20300.16530190805631012No Hit
GGCACAGAGTAGTTGTTGAACATGGATGAATGAAACCTGTCGAGTGAGAG20260.164976190010879No Hit
CACAAATTCATTCCACTCTCACTCGACAGGTTTCATTCATCCATGTTCAA20230.1647319014768056No Hit
GCCTGAAGATCTGCATGAGAACCAATGCACACCAGGGCTATGCTGCCTCC20070.16342902929508102No Hit
AGCATGGGCTGTGCCCAGCACATGCTGAGACTCGGGGAGTGGGGAGGCAT19910.1621261571133564No Hit
AGATCACCCTGGGGTTTTGAGCTTGGGAGACTGGAGAGATGAGGAATCCT19880.16188186857928302No Hit
CACAGATACTGCTGGGAGCCGAGGGAAGGCAATGCTGAGGTCCCTCCCTG19780.16106757346570516No Hit
CTAATAACCCGCCTTGTTTCTACTAATAACCCGCCTTGTTTCTACTAATA19760.16090471444298957No Hit
TCTGTGGGGTGATGCCACAGTGGGCTCTGCTCTTTCTCCAAAGTATGGAT19290.15707752740917352No Hit
GAGTAAAAGTCGGTCACTGCCCTGCTGCTGAGTGGGAATCCTGGAGTGCT19130.15577465522744893No Hit
CCCCACCACCCTCCTAGCGCAGCACTCCAGGATTCCCACTCAGCAGCAGG18990.15463464206843985No Hit
GCATATTCTCGTCTCCAGCACACAAATTCATTCCACTCTCACTCGACAGG18840.15341319939807307No Hit
ATTCTCGTCTCCAGCACACAAATTCATTCCACTCTCACTCGACAGGTTTC18790.1530060518412841No Hit
CTCTGTGACAACAAACATTTGGTGAATTGAACTAAAAAGCTGGGTTCCCA18550.15105174356869722No Hit
AGGTGGGAGAGGGCAGAGGGCAGACTGAGCTCCAGGGGACTTGGCTGCAA18400.14983030089833038No Hit
GTATGGATGCCTCCCCACTCCCCGAGTCTCAGCATGTGCTGGGCACAGCC18390.1497488713869726No Hit
GAGGTGGGAGAGGGCAGAGGGCAGACTGAGCTCCAGGGGACTTGGCTGCA18330.14926029431882587No Hit
GTCACAGAGCCTGTGCAGCTGTGAACAGTGGGCTCCACCTGACACCCACA18160.14787599262574344No Hit
GACCAGGCCCTGGGGACCACTTGGTCACGGAGGCAGCATAGCCCTGGTGT17870.14551453679636758No Hit
GGAGATCACCCTGGGGTTTTGAGCTTGGGAGACTGGAGAGATGAGGAATC17810.14502595972822085No Hit
ACCCTGGGGTTTTGAGCTTGGGAGACTGGAGAGATGAGGAATCCTGGACG17790.1448631007055053No Hit
GCATATGTCTTCTCCATCATCTGTTGGGTTTGCAGCCAAGTCCCCTGGAG17740.14445595314871634No Hit
GGTGTGCATTGGTTCTCATGCAGATCTTCAGGCTGGGAGTAAAAGTCGGT17640.14364165803513845No Hit
ATCCTGGAGTGCTGCGCTAGGAGGGTGGTGGGGAGAGGTGGGAGAGGGCA17630.14356022852378067No Hit
CCCGGGCACAGAGTAGTTGTTGAACATGGATGAATGAAACCTGTCGAGTG17550.14290879243291837No Hit
GTTCAACAACTACTCTGTGCCCGGGCAACCAGGATTGGGAACCCAGCTTT17430.14193163829662492No Hit
CCAGAGCATGGGCTGTGCCCAGCACATGCTGAGACTCGGGGAGTGGGGAG17210.14014018904675357No Hit
ACTCTGTGCCCGGGCAACCAGGATTGGGAACCCAGCTTTTTAGTTCAATT17140.13957018246724906No Hit
CATTTGGTGAATTGAACTAAAAAGCTGGGTTCCCAATCCTGGTTGCCCGG17140.13957018246724906No Hit
GTGCCCAGCACATGCTGAGACTCGGGGAGTGGGGAGGCATCCATACTTTG17130.13948875295589125No Hit
GTGCAGCTGTGAACAGTGGGCTCCACCTGACACCCACACCCTCAGGGAGG17120.13940732344453347No Hit
GCACATGCTGAGACTCGGGGAGTGGGGAGGCATCCATACTTTGGAGAAAG16980.13826731028552444No Hit
CTCAGGGAGGGACCTCAGCATTGCCTTCCCTCGGCTCCCAGCAGTATCTG16960.13810445126280885No Hit
GTTTTGAGCTTGGGAGACTGGAGAGATGAGGAATCCTGGACGGGAGCAAG16080.13093865426332352No Hit
CCCTCCTAGCGCAGCACTCCAGGATTCCCACTCAGCAGCAGGGCAGTGAC15920.1296357820815989No Hit
AGTCTGTGGTGGAAGCGGAGATGGTGGTGAGACCAGGCCCTGGGGACCAC15810.1287400574566632No Hit
AGCGAAAGCAGGCTAGGAACCTAGGTCTCTGCACTCCAGAGCATGGGCTG15760.12833290989987428No Hit
ATGTTCAACAACTACTCTGTGCCCGGGCAACCAGGATTGGGAACCCAGCT15640.12735575576358082No Hit
GCTGGGAGCCGAGGGAAGGCAATGCTGAGGTCCCTCCCTGAGGGTGTGGG15560.1267043196727185No Hit
GTTGAACATGGATGAATGAAACCTGTCGAGTGAGAGTGGAATGAATTTGT15450.12580859504778283No Hit
GCCCACTGTGGCATCACCCCACAGATACTGCTGGGAGCCGAGGGAAGGCA15290.12450572286605821No Hit
CTGGAGAGATGAGGAATCCTGGACGGGAGCAAGTCTGTGGTGGAAGCGGA15120.12312142117297584No Hit
ACTAATAACCCGCCTTGTTTCTACTAATAACCCGCCTTGTTTCTACTAAT15080.12279570312754466No Hit
CTGCACTCCAGAGCATGGGCTGTGCCCAGCACATGCTGAGACTCGGGGAG15010.12222569654804015No Hit
TCCATGTTCAACAACTACTCTGTGCCCGGGCAACCAGGATTGGGAACCCA14940.12165568996853562No Hit
ATGCCACAGTGGGCTCTGCTCTTTCTCCAAAGTATGGATGCCTCCCCACT14890.1212485424117467No Hit
CTCAGCAGCAGGGCAGTGACCGACTTTTACTCCCAGCCTGAAGATCTGCA14870.12108568338903111No Hit
CATACATGCAGCCTCAGTTTTGGTGATGTTATTTTTCAAGCTGGGTGGCA14770.12027138827545325No Hit
CTGTGACAACAAACATTTGGTGAATTGAACTAAAAAGCTGGGTTCCCAAT14770.12027138827545325No Hit
CACCAAATGTTTGTTGTCACAGAGCCTGTGCAGCTGTGAACAGTGGGCTC14590.11880565707101305No Hit
GCTGAGGTCCCTCCCTGAGGGTGTGGGTGTCAGGTGGAGCCCACTGTTCA14550.1184799390255819No Hit
GTATCTGTGGGGTGATGCCACAGTGGGCTCTGCTCTTTCTCCAAAGTATG14460.1177470734233618No Hit
GGGCAGAGGGCAGACTGAGCTCCAGGGGACTTGGCTGCAAACCCAACAGA14430.11750278488928843No Hit
CCCAACAGATGATGGAGAAGACATATGCCTTCCAGCATATTCTCGTCTCC14340.11676991928706835No Hit
CTCCCTGAGGGTGTGGGTGTCAGGTGGAGCCCACTGTTCACAGCTGCACA14110.11489704052583923No Hit
GGAGAAGACATATGCCTTCCAGCATATTCTCGTCTCCAGCACACAAATTC14080.11465275199176585No Hit
GACTCGGGGAGTGGGGAGGCATCCATACTTTGGAGAAAGAGCAGAGCCCA14050.1144084634576925No Hit
ATGCACACCAGGGCTATGCTGCCTCCGTGACCAAGTGGTCCCCAGGGCCT14020.11416417492361912No Hit
CCCACACCCTCAGGGAGGGACCTCAGCATTGCCTTCCCTCGGCTCCCAGC13960.11367559785547239No Hit
GAACCTAGGTCTCTGCACTCCAGAGCATGGGCTGTGCCCAGCACATGCTG13900.11318702078732566No Hit
ATACTGCTGGGAGCCGAGGGAAGGCAATGCTGAGGTCCCTCCCTGAGGGT13850.11277987323053672No Hit
GCCCTGGGGACCACTTGGTCACGGAGGCAGCATAGCCCTGGTGTGCATTG13770.11212843713967441No Hit
CCCCAGGGCCTGGTCTCACCACCATCTCCGCTTCCACCACAGACTTGCTC13620.11090699446930757No Hit
GGGTGTGGGTGTCAGGTGGAGCCCACTGTTCACAGCTGCACAGGCTCTGT13580.11058127642387644No Hit
GTTTCTACTAATAACCCGCCTTGTTTCTACTAATAACCCGCCTTGTTTCT13480.10976698131029855No Hit
GCATGGGCTGTGCCCAGCACATGCTGAGACTCGGGGAGTGGGGAGGCATC13460.10960412228758298No Hit
GATACTGCTGGGAGCCGAGGGAAGGCAATGCTGAGGTCCCTCCCTGAGGG13330.10854553863993172No Hit
CCCAACAGATGATGGAGAAGACATATGCCTTCCGGCATATTCTCGTCTCC13270.10805696157178499No Hit
CTGTGGGGTGATGCCACAGTGGGCTCTGCTCTTTCTCCAAAGTATGGATG13150.10707980743549154No Hit
ACCCCACAGATACTGCTGGGAGCCGAGGGAAGGCAATGCTGAGGTCCCTC13090.10659123036734482No Hit
GTGGTGGGGAGAGGTGGGAGAGGGCAGAGGGCAGACTGAGCTCCAGGGGA13080.10650980085598702No Hit
ATATTCTCGTCTCCAGCACACAAATTCATTCCACTCTCACTCGACAGGTT13050.10626551232191365No Hit
TCATTCATCCATGTTCAACAACTACTCTGTGCCCGGGCAACCAGGATTGG13050.10626551232191365No Hit
AAACAAGGCGGGTTATTAGTAGAAACAAGGCGGGTTATTAGTAGAAACAA13040.10618408281055586No Hit
GCTTTTTAGTTCAATTCACCAAATGTTTGTTGTCACAGAGCCTGTGCAGC12950.10545121720833578No Hit
GAGCAGAGCCCACTGTGGCATCACCCCACAGATACTGCTGGGAGCCGAGG12920.10520692867426241No Hit
ATGCTGAGGTCCCTCCCTGAGGGTGTGGGTGTCAGGTGGAGCCCACTGTT12860.10471835160611569No Hit
GGTGAGACCAGGCCCTGGGGACCACTTGGTCACGGAGGCAGCATAGCCCT12760.1039040564925378No Hit
ATTCCACTCTCACTCGACAGGTTTCATTCATCCATGTTCAACAACTACTC12750.10382262698118No Hit
CTAAAAAGCTGGGTTCCCAATCCTGGTTGCCCGGGCACAGAGTAGTTGTT12650.10300833186760214No Hit
GAATGAAACCTGTCGAGTGAGAGTGGAATGAATTTGTGTGCTGGAGACGA12630.10284547284488656No Hit
TCTGTTGGGTTTGCAGCCAAGTCCCCTGGAGCTCAGTCTGCCCTCTGCCC12600.10260118431081318No Hit
GGAGCAAGTCTGTGGTGGAAGCGGAGATGGTGGTGAGACCAGGCCCTGGG12450.10137974164044637No Hit
GTTGTTGAACATGGATGAATGAAACCTGTCGAGTGAGAGTGGAATGAATT12350.10056544652686848No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)