FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8700147
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATAACTATCCAAACTCACATAATACTGGGACACCTGGGTGACTCAG645350.7417690758558447No Hit
GTATTTAACATGGGAGATGTTTAATTTGGTCCCGGCAAAATTTTGAATTC513790.5905532400774378No Hit
AAATAAGTGTATGTGGCATACCAAAAGTGCTTAAATATTATTGCTTCCAC415660.4777620424114673No Hit
GTTAAATACTATGGCTTAAAACATTAAGCCACCAATACCAAGATTTATTG337320.38771758684077406No Hit
GTCAGAGAGAGAGCAAAAGAGAGAGAGAAAGAGAGCAAGAGCGCGAGCAA336390.38664863938505867No Hit
GTATATATATAACTATCCAAACTCACATAATACTGGGACACCTGGGTGAC305070.35064924765064315No Hit
GAATATAGTCAAAGCAGTGGATACAAAGTGTCAGATACTGCTCCAGGCCA290700.3341322853510406No Hit
CAATAATGGAGGAGTGGCAGGCAGAGGTAGAAGTAGGCTCCCTGCTGAGC270770.31122462643447285No Hit
GTCAGATACTGCTCCAGGCCATAGCAATCTTCTCCAAATTCTTCTGGTCA262570.3017994983303156No Hit
GTATTACATATTTCCATGGGAGCAGTATGATGATACAAGTATGCCTCATT257520.2959949987051943No Hit
CCATAAAAGAGCATAAAAATGAATATAACTGAAGCAAACCTGAGTTAGAA253470.2913399049464337No Hit
ATATTATTGCTTCCACTATCAGGATTATTACCACCACCAAAAGGTGGCAT249560.2868457280089635No Hit
GTGAAAGATAGCCCATAGTACCTACTAGTTCCTACTCTTATTTCTCAGTC244310.2808113472105701No Hit
CACTTATTTAGTCCTCAAATATGTCTAAGAAATAGGTACTGTTCTTTTTC243130.27945504828826456No Hit
ATACTGGGACACCTGGGTGACTCAGTTGGTTAAGCACCTGCCTTCAGCTC238840.2745240971215773No Hit
ATGTAATACCGATACATTTTAACTTGGCCTAAGGGATTTTTAAAATTATG234140.26912188954968236No Hit
CAATAAATCTTGGTATTGGTGGCTTAATGTTTTAAGCCATAGTATTTAAC232410.26713341740087837No Hit
ACCTAAGTGCATAGCTAATAGTTCTATTTAAGGAAAGGAGAATACACAGA230010.2643748433216128No Hit
ATAATAACTAGGATGCAGTCAGCTACACTCAATTCTCTAGTTAATATACT229210.26345531862852434No Hit
GTAGAGACAGTGAGTAATAGGGTGTTTGATAGTTTGTGAAGAAATGTGAA216720.24909923935767983No Hit
GTACCTACTAGTTCCTACTCTTATTTCTCAGTCTTTTCATCTGTAAAATG213570.24547861087864378No Hit
TACCAAAAGTGCTTAAATATTATTGCTTCCACTATCAGGATTATTACCAC209260.24052467159462937No Hit
GTTAATATACTTTGTTGGCACATCAGCCTTGAAATACCCAGAATTCAAAA206520.23737529952080122No Hit
GTAGTAATGTAGATTACTTTTTAGTAAAGGTAGTTTACTTGTCAGTTGAT204590.23515694619872513No Hit
GCTAATAGTTCTATTTAAGGAAAGGAGAATACACAGAGAATATAGTCAAA200400.23034093561867405No Hit
GTACTATTTGATATGATGACTTAGAAAATTTTTGTTCCATTCTTCTAACT199070.2288122258164144No Hit
TTATTTATGTATGCAATAAATCTTGGTATTGGTGGCTTAATGTTTTAAGC196070.2253640082173324No Hit
GTAATAGGGTGTTTGATAGTTTGTGAAGAAATGTGAATCTTCCAAGTTCC194880.22399621523636323No Hit
CACATAATACTGGGACACCTGGGTGACTCAGTTGGTTAAGCACCTGCCTT188260.21638714840105577No Hit
GTATGATGATACAAGTATGCCTCATTGGATAAAAGCCACTAGCCCCATTC186840.2147549920708236No Hit
CATAAATAATGAAACTACCTAAGTGCATAGCTAATAGTTCTATTTAAGGA183910.21138723288238692No Hit
AAATAGTACAATGCTTTTCCAGTTTTTTTCTTACTCCTTACACCCAAAAA178950.2056861797852381No Hit
TCATCATACTGCTCCCATGGAAATATGTAATACCGATACATTTTAACTTG177450.20396207098569713No Hit
GGATTATTACCACCACCAAAAGGTGGCATTGATGACCAGAAGAATTTGGA175470.20168624737030308No Hit
ATCCATAAAAGAGCATAAAAATGAATATAACTGAAGCAAACCTGAGTTAG173880.19985869204278964No Hit
GTATATTAACTAGAGAATTGAGTGTAGCTGACTGCATCCTAGTTATTATT170300.1957438190412185No Hit
GTGTATAATGATAAAATCTGTACTTTAAAACAAGCAAAATTAAGTTGTCA170220.19565186657190967No Hit
AGTCAAAGCAGTGGATACAAAGTGTCAGATACTGCTCCAGGCCATAGCAA167400.1924105420287726No Hit
CTAAATAAGTGTATGTGGCATACCAAAAGTGCTTAAATATTATTGCTTCC166790.1917094044502926No Hit
GCTTTGTACCCAGTAGAGACAGTGAGTAATAGGGTGTTTGATAGTTTGTG162240.18647960775835165No Hit
ACTACAGACCTAATAAAATCAATATTTCTATGAATGGGGCTAGTGGCTTT160770.18478998113480152No Hit
GTTTCTAATCTTATGATGCAAATTAAACCAATAAGGAACAAAAGTCACTG160350.18430723067093005No Hit
CCTTAAGACTCTTCATCTTTAAAAATTTCATTAAATTTCGGGGCGCCTGG158960.18270955651668874No Hit
AGTTATTATTATCCAACAGTGACTTTTGTTCCTTATTGGTTTAATTTGCA156240.17958317256018777No Hit
TTTCAAGGCTGATGTGCCAACAAAGTATATTAACTAGAGAATTGAGTGTA154460.17753723011806583No Hit
GTGTCAGATACTGCTCCAGGCCATAGCAATCTTCTCCAAATTCTTCTGGT154310.1773648192381117No Hit
TATTCATACAGATGATGTGCCAAATCTCACAGAATATTTGGCAAAACACT154220.17726137271013928No Hit
GTATCTGACACTTTGTATCCACTGCTTTGACTATATTCTCTGTGTATTCT154010.17701999747820354No Hit
GAGTAATAGGGTGTTTGATAGTTTGTGAAGAAATGTGAATCTTCCAAGTT152660.17546829955861665No Hit
ATTTGATATGATGACTTAGAAAATTTTTGTTCCATTCTTCTAACTCAGGT151880.17457176298285534No Hit
GCATTAACCACTGGCTTTCTCAAGGCACCATCAGTCACAAAGAAACAGTT151660.174318893692256No Hit
ATACAGATGATGTGCCAAATCTCACAGAATATTTGGCAAAACACTCAGTG151220.17381315511105733No Hit
ACATTACTACAGACCTAATAAAATCAATATTTCTATGAATGGGGCTAGTG151130.17370970858308488No Hit
AAAATAAACTCACTTTAGTTAAATCTGCAAAAACCAGCATTAACCACTGG148420.1705948186852475No Hit
GTTTTAAGCCATAGTATTTAACATGGGAGATGTTTAATTTGGTCCCGGCA144270.1658247843398508No Hit
GTGCATAGCTAATAGTTCTATTTAAGGAAAGGAGAATACACAGAGAATAT144100.1656293853425695No Hit
GTGTATGTGGCATACCAAAAGTGCTTAAATATTATTGCTTCCACTATCAG141100.16218116774348754No Hit
GTTTAATTTGGTCCCGGCAAAATTTTGAATTCTGGGTATTTCAAGGCTGA140550.1615489945169892No Hit
CCATAGTACCTACTAGTTCCTACTCTTATTTCTCAGTCTTTTCATCTGTA140040.16096279752514528No Hit
CATTACTACAGACCTAATAAAATCAATATTTCTATGAATGGGGCTAGTGG139550.16039958865062853No Hit
GTATGTGGCATACCAAAAGTGCTTAAATATTATTGCTTCCACTATCAGGA137950.15856053926445152No Hit
AGTTAATATACTTTGTTGGCACATCAGCCTTGAAATACCCAGAATTCAAA137810.158399622443161No Hit
CCCTATTACTCACTGTCTCTACTGGGTACAAAGCACTGTGAAGAATATAA137810.158399622443161No Hit
ACTATTAGCTATGCACTTAGGTAGTTTCATTATTTATGTATGCAATAAAT136340.15670999581961087No Hit
GCCTTGAAATACCCAGAATTCAAAATTTTGCCGGGACCAAATTAAACATC136220.15657206711564758No Hit
ACATAATACTGGGACACCTGGGTGACTCAGTTGGTTAAGCACCTGCCTTC135860.15615828100375775No Hit
CTTCAGTACATAATTTTAAAAATCCCTTAGGCCAAGTTAAAATGTATCGG135720.15599736418246726No Hit
GTACTGAAGTGATAAAATTAAAATAAACTAAGAGCCTGGAAATCTAGGAA134090.1541238326202994No Hit
CACTTAGGTAGTTTCATTATTTATGTATGCAATAAATCTTGGTATTGGTG132860.1527100634046758No Hit
CTGTCTCTACTGGGTACAAAGCACTGTGAAGAATATAAAAGAAAAAAGTA131560.15121583577840697No Hit
CAATAATATTTAAGCACTTTTGGTATGCCACATACACTTATTTAGTCCTC130210.1496641378588201No Hit
ATATCAAATAGTACAATGCTTTTCCAGTTTTTTTCTTACTCCTTACACCC128840.148089451821906No Hit
GGACATGGGAGCACAATAATGGAGGAGTGGCAGGCAGAGGTAGAAGTAGG128700.1479285350006155No Hit
GCTCCAGGCCATAGCAATCTTCTCCAAATTCTTCTGGTCATCAATGCCAC127670.14674464695826403No Hit
GTTAAAATGTATCGGTATTACATATTTCCATGGGAGCAGTATGATGATAC121820.14002062264005424No Hit
CTACTAAACTCTTTTCCTATAAGGAACTTGGAAGATTCACATTTCTTCAC121430.13957235435217358No Hit
GTTAAGCACCTGCCTTCAGCTCAGGGCTTGATCCGGAGTCCCATATCTGG120300.13827352572318605No Hit
GAGAATACACAGAGAATATAGTCAAAGCAGTGGATACAAAGTGTCAGATA119810.13771031684866933No Hit
TCCCAGGACCCTGAGCCAAAGGAGATGCTCAACCATCTGAGCCACCCAGG119290.1371126257981618No Hit
GTCTTAAGGGATAAAATTAGTTTAAAGGCTTTACAAATAAATTAATAAAC118710.1364459703956726No Hit
GATTTATTGCATACATAAATAATGAAACTACCTAAGTGCATAGCTAATAG118680.1364114882196818No Hit
ATTATACACAATTCCATCTCAGAATGCATCCTGTTTATTAATTTATTTGT116860.13431956954290544No Hit
CCATTATTGTGCTCCCATGTCCCCTGCTGCTGTGCTTGCTCGCGCTCTTG116590.13400922995898804No Hit
ACCTACTAGTTCCTACTCTTATTTCTCAGTCTTTTCATCTGTAAAATGGA116540.13395175966567002No Hit
ATACTGCTCCAGGCCATAGCAATCTTCTCCAAATTCTTCTGGTCATCAAT114920.13208972216216575No Hit
ATCTGACACTTTGTATCCACTGCTTTGACTATATTCTCTGTGTATTCTCC114040.1310782449997684No Hit
GTACAAAGCACTGTGAAGAATATAAAAGAAAAAAGTAGTATTTGTAAATT113270.1301932024826707No Hit
CTTATTTAGTCCTCAAATATGTCTAAGAAATAGGTACTGTTCTTTTTCCA113230.13014722624801628No Hit
AGTAGGTACTATGGGCTATCTTTCACTGAGTGTTTTGCCAAATATTCTGT112850.12971045201879922No Hit
GCTATGCACTTAGGTAGTTTCATTATTTATGTATGCAATAAATCTTGGTA112190.1289518441470012No Hit
ACCTATTTCTTAGACATATTTGAGGACTAAATAAGTGTATGTGGCATACC112120.12887138573635595No Hit
CTTTTGGTATGCCACATACACTTATTTAGTCCTCAAATATGTCTAAGAAA111590.1282622006271848No Hit
GTTTGATAGTTTGTGAAGAAATGTGAATCTTCCAAGTTCCTTATAGGAAA111410.1280553075712399No Hit
GAATATAACTGAAGCAAACCTGAGTTAGAAGAATGGAACAAAAATTTTCT111080.12767600363534087No Hit
GTGAGTAATAGGGTGTTTGATAGTTTGTGAAGAAATGTGAATCTTCCAAG110520.1270323363501789No Hit
GTACAATGCTTTTCCAGTTTTTTTCTTACTCCTTACACCCAAAAAATGAC110240.12671050270759793No Hit
ATTTAAGGAAAGGAGAATACACAGAGAATATAGTCAAAGCAGTGGATACA109040.12533121566796515No Hit
GCATTGTACTATTTGATATGATGACTTAGAAAATTTTTGTTCCATTCTTC108300.12448065532685826No Hit
ATTCTCTAGTTAATATACTTTGTTGGCACATCAGCCTTGAAATACCCAGA107690.12377951774837828No Hit
GGAGAATACACAGAGAATATAGTCAAAGCAGTGGATACAAAGTGTCAGAT106510.12242321882607271No Hit
GATAAAATCTGTACTTTAAAACAAGCAAAATTAAGTTGTCATTTTTTGGG106180.12204391489017369No Hit
GGTAGAAGTAGGCTCCCTGCTGAGCAAGGAGCCAGATATGGGACTCCGGA105340.12107841396243074No Hit
GTCTGTAGTAATGTAGATTACTTTTTAGTAAAGGTAGTTTACTTGTCAGT105330.12106691990376715No Hit
GATACAAAGTGTCAGATACTGCTCCAGGCCATAGCAATCTTCTCCAAATT101670.11686009443288717No Hit
ATCTTTCACTGAGTGTTTTGCCAAATATTCTGTGAGATTTGGCACATCAT101480.11664170731827865No Hit
CTTTAAAACAAGCAAAATTAAGTTGTCATTTTTTGGGTGTAAGGAGTAAG100400.11540034898260913No Hit
TAATAATCCTGATAGTGGAAGCAATAATATTTAAGCACTTTTGGTATGCC100260.11523943216131866No Hit
TTTGAGGACTAAATAAGTGTATGTGGCATACCAAAAGTGCTTAAATATTA99900.11482564604942881No Hit
ATATTAACTAGAGAATTGAGTGTAGCTGACTGCATCCTAGTTATTATTAT99770.11467622328680194No Hit
AATTAAACCAATAAGGAACAAAAGTCACTGTTGGATAATAATAACTAGGA98290.11297510260458818No Hit
ATATGGGACTCCGGATCAAGCCCTGAGCTGAAGGCAGGTGCTTAACCAAC96970.11145788686099212No Hit
GCATCAGAATCATCTGGGATCACTTGGAGAAGTAAACTACAGATTCTGCA96780.1112394997463836No Hit
GTTATATATATACAATGAACTAATTATAAAGGAATATACAATGAAAGTTA96690.11113605321841112No Hit
GTACCTATTTCTTAGACATATTTGAGGACTAAATAAGTGTATGTGGCATA96230.11060732651988524No Hit
CTGTTGGATAATAATAACTAGGATGCAGTCAGCTACACTCAATTCTCTAG96030.1103774453466131No Hit
CATTTTAACTTGGCCTAAGGGATTTTTAAAATTATGTACTGAAGTGATAA95970.11030848099463147No Hit
CTATTACTCACTGTCTCTACTGGGTACAAAGCACTGTGAAGAATATAAAA95040.10923953353891606No Hit
AACTAAGAGCCTGGAAATCTAGGAATCCCTACTAAACTCTTTTCCTATAA95030.10922803948025246No Hit
ACATATTTCCATGGGAGCAGTATGATGATACAAGTATGCCTCATTGGATA94860.10903264048297116No Hit
ACTATATTCTCTGTGTATTCTCCTTTCCTTAAATAGAACTATTAGCTATG94750.10890620583767147No Hit
TTCCTACTCTTATTTCTCAGTCTTTTCATCTGTAAAATGGAAAAAGAACA94470.10858437219509051No Hit
GAAATAGGTACTGTTCTTTTTCCATTTTACAGATGAAAAGACTGAGAAAT93930.10796369302725575No Hit
TATTTGAGGACTAAATAAGTGTATGTGGCATACCAAAAGTGCTTAAATAT93580.10756140097402953No Hit
ATATTTGGCAAAACACTCAGTGAAAGATAGCCCATAGTACCTACTAGTTC92280.10606717334776068No Hit
ATTTATTTGTCAGAGAGAGAGCAAAAGAGAGAGAGAAAGAGAGCAAGAGC92220.10599820899577905No Hit
GTCCCATATCTGGCTCCTTGCTCAGCAGGGAGCCTACTTCTACCTCTGCC91410.10506719024402691No Hit
CTATCAGGATTATTACCACCACCAAAAGGTGGCATTGATGACCAGAAGAA90810.10437754672421053No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT90480.1039982427883115No Hit
ATTCTTCACAGTGCTTTGTACCCAGTAGAGACAGTGAGTAATAGGGTGTT89920.10335457550314955No Hit
ATTACTCACTGTCTCTACTGGGTACAAAGCACTGTGAAGAATATAAAAGA89530.1029063072152689No Hit
GGATACAAAGTGTCAGATACTGCTCCAGGCCATAGCAATCTTCTCCAAAT89190.10251550922070628No Hit
GGCCATAGCAATCTTCTCCAAATTCTTCTGGTCATCAATGCCACCTTTTG88910.10219367557812528No Hit
TACTAGTTCCTACTCTTATTTCTCAGTCTTTTCATCTGTAAAATGGAAAA88450.10166494887959938No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG88010.10115921029840072No Hit
CTTTAGTTAAATCTGCAAAAACCAGCATTAACCACTGGCTTTCTCAAGGC87990.1011362221810735No Hit
GTGTATTCTCCTTTCCTTAAATAGAACTATTAGCTATGCACTTAGGTAGT87130.10014773313600334No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)