FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A7a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A7a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences973809
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAGAAGGAAGAACC89890.9230762911412813No Hit
AGCGAAAGCAGGCCAGCTTGCCGGAGTTCGTCTCCAAACGGAATTTCTGA69120.7097901128455375No Hit
GTATCCGAGCTTCTCCCCCTTCTCCCCTGACAACGTCACTGAAAAGCGAT67440.6925382698249862No Hit
GTACTGCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAGAAGGAA66210.6799074561849398No Hit
TCTTTGGACTTCTGCTTTCGCCTCTCCTCACAAACACGGGAGAGGCTGCA63890.6560834824898928No Hit
GCCCTGCATCTCGGATCGTGATTCCTCATTTAGAAGGAAGAACCAAATCT49800.5113939181091981No Hit
CCCCTGACAACGTCACTGAAAAGCGATGTTCTTTGGACTTCTGCTTTCGC48610.4991738626363075No Hit
AAGCAGGCCAGCTTGCCGGAGTTCGTCTCCAAACGGAATTTCTGAATGTA47610.48890490845740797No Hit
GTCCAAAAGATGTGCAGCCTCTCCCGTGTTTGTGAGGAGAGGCGAAAGCA44170.45357970608199355No Hit
AGCAGGAGCAGTGGCAGCAGCAGGAGCAGCAGCAGGAGCAGCAGCAGGAG42960.441154271525525No Hit
ACCAAATCTTGTCCAAAAGATGTGCAGCCTCTCCCGTGTTTGTGAGGAGA42690.43838165389722217No Hit
GTCCAAAGAACATCGCTTTTCAGTGACGTTGTCAGGGGAGAAGGGGGAGA40580.4167141605797441No Hit
CCTCACAAACACGGGAGAGGCTGCACATCTTTTGGACAAGATTTGGTTCT35430.36382904655841136No Hit
AGCGAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTA29290.30077766789996807No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAACTACTAGTATTG28670.2944109163090503No Hit
CATTTAGAAGGAAGAACCAAATCTTGTCCAAAAGATGTGCAGCCTCTCCC28580.2934867104329494No Hit
CTTCTCCCCTGACAACGTCACTGAAAAGCGATGTTCTTTGGACTTCTGCT27020.27746714191386607No Hit
ATCCGAGCTTCTCCCCCTTCTCCCCTGACAACGTCACTGAAAAGCGATGT26750.2746945242855632No Hit
AGATTTGGTTCTTCCTTCTAAATGAGGAATCACGATCCGAGATGCAGGGC26630.2734622497840952No Hit
GGCCAGCTTGCCGGAGTTCGTCTCCAAACGGAATTTCTGAATGTACTGCC26170.26873853086180144No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA25840.2653497759827646No Hit
GAGCAGGAGCAGTGGCAGCGGGCAGTATCCGAGCTTCTCCCCCTTCTCCC25810.26504170735739757No Hit
CTCCCGTGTTTGTGAGGAGAGGCGAAAGCAGAAGTCCAAAGAACATCGCT25420.2610368152276268No Hit
GAATTTCTGAATGTACTGCCCCAGCCCTGCATCTCGGATCGTGATTCCTC22710.23320794940280895No Hit
CTTCTCCCCCTTCTCCCCTGACAACGTCACTGAAAAGCGATGTTCTTTGG22650.23259181215207497No Hit
GGCAGTATCCGAGCTTCTCCCCCTTCTCCCCTGACAACGTCACTGAAAAG22450.23053802131629508No Hit
CGGGAGAGGCTGCACATCTTTTGGACAAGATTTGGTTCTTCCTTCTAAAT22260.22858692002230416No Hit
CTGAATGTACTGCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAG22080.22673850827010225No Hit
GATCGTGATTCCTCATTTAGAAGGAAGAACCAAATCTTGTCCAAAAGATG22030.22622506056115727No Hit
TTTGTGAGGAGAGGCGAAAGCAGAAGTCCAAAGAACATCGCTTTTCAGTG21980.2257116128522123No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT21980.2257116128522123No Hit
GCCTCTCCCGTGTTTGTGAGGAGAGGCGAAAGCAGAAGTCCAAAGAACAT21660.22242554751496446No Hit
ATTTAGAAGGAAGAACCAAATCTTGTCCAAAAGATGTGCAGCCTCTCCCG21380.21955024034487255No Hit
GAACCAAATCTTGTCCAAAAGATGTGCAGCCTCTCCCGTGTTTGTGAGGA21020.21585341684046871No Hit
TCTTTTGGACAAGATTTGGTTCTTCCTTCTAAATGAGGAATCACGATCCG20980.21544265867331275No Hit
CTTTTGGACAAGATTTGGTTCTTCCTTCTAAATGAGGAATCACGATCCGA20590.2114377665435419No Hit
TCTCCCGTGTTTGTGAGGAGAGGCGAAAGCAGAAGTCCAAAGAACATCGC20030.20568715220335815No Hit
GTAGAAACAAGGGTGTTTTTCCTCATATTTCTGAAATTCTAATCTTAAAT19990.20527639403620218No Hit
GCTTTCGCCTCTCCTCACAAACACGGGAGAGGCTGCACATCTTTTGGACA19330.19849888427812845No Hit
ACACGGGAGAGGCTGCACATCTTTTGGACAAGATTTGGTTCTTCCTTCTA18890.19398054443941265No Hit
CCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAGAAGGAAGAACCA18890.19398054443941265No Hit
CACAAACACGGGAGAGGCTGCACATCTTTTGGACAAGATTTGGTTCTTCC18350.18843530918280688No Hit
CACGGGAGAGGCTGCACATCTTTTGGACAAGATTTGGTTCTTCCTTCTAA18070.18556000201271503No Hit
GCTTGCCGGAGTTCGTCTCCAAACGGAATTTCTGAATGTACTGCCCCAGC17940.18422503796945808No Hit
GTTCTTTGGACTTCTGCTTTCGCCTCTCCTCACAAACACGGGAGAGGCTG17930.18412234842766906No Hit
GTTTGTGAGGAGAGGCGAAAGCAGAAGTCCAAAGAACATCGCTTTTCAGT17720.18196586805010018No Hit
GATTTGGTTCTTCCTTCTAAATGAGGAATCACGATCCGAGATGCAGGGCT17630.18104166217399922No Hit
AACCAAATCTTGTCCAAAAGATGTGCAGCCTCTCCCGTGTTTGTGAGGAG17580.18052821446505424No Hit
GCATCTCGGATCGTGATTCCTCATTTAGAAGGAAGAACCAAATCTTGTCC17320.17785828637854037No Hit
GTGCAGCCTCTCCCGTGTTTGTGAGGAGAGGCGAAAGCAGAAGTCCAAAG16980.1743668419577145No Hit
GTGTTTGTGAGGAGAGGCGAAAGCAGAAGTCCAAAGAACATCGCTTTTCA16980.1743668419577145No Hit
ATCTCGGATCGTGATTCCTCATTTAGAAGGAAGAACCAAATCTTGTCCAA16930.17385339424876953No Hit
GTAGAGACCCATTAGAGCACATCCAGAACTACTAGTATTGCCTTCATTTT16840.17292918837266855No Hit
GAAAAGCGATGTTCTTTGGACTTCTGCTTTCGCCTCTCCTCACAAACACG16670.17118346616225563No Hit
CCCTTCTCCCCTGACAACGTCACTGAAAAGCGATGTTCTTTGGACTTCTG16570.1701565707443657No Hit
CTTAAATACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAC16220.16656243678175084No Hit
AGCAGGAGCAGTGGCAGCGGGCAGTATCCGAGCTTCTCCCCCTTCTCCCC16220.16656243678175084No Hit
CTGAAAAGCGATGTTCTTTGGACTTCTGCTTTCGCCTCTCCTCACAAACA15400.1581418943550532No Hit
TCTGAATGTACTGCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTA15320.15732037802074123No Hit
CTCCTCACAAACACGGGAGAGGCTGCACATCTTTTGGACAAGATTTGGTT15210.1561907930610623No Hit
GATGTGCAGCCTCTCCCGTGTTTGTGAGGAGAGGCGAAAGCAGAAGTCCA15170.1557800348939063No Hit
GGCGAAAGCAGAAGTCCAAAGAACATCGCTTTTCAGTGACGTTGTCAGGG14840.15239128001486943No Hit
AAACAAGGGTGTTTTTCCTCATATTTCTGAAATTCTAATCTTAAATACAT14820.15218590093129145No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAACTACTAGTATTGCCTTC14580.14972135192835556No Hit
TGTGAGGAGAGGCGAAAGCAGAAGTCCAAAGAACATCGCTTTTCAGTGAC14310.14694873430005267No Hit
AATAAAAACAACCAAAATGAAGGCAATACTAGTAGTTCTGGATGTGCTCT14180.14561377025679573No Hit
TACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAACTACTAG14170.14551108071500674No Hit
AGCGAAAGCAGGAGCAGTGGCAGCAGCAGGAGCAGCAGCAGGAGCAGCAG13940.14314922125385984No Hit
GAGCAGCAGCAGGAGCAGCAGCAGGAGCAGGAGCAGTGGCAGCGGGCAGT13920.14294384217028186No Hit
CATTAGAGCACATCCAGAACTACTAGTATTGCCTTCATTTTGGTTGTTTT13810.1418142572106029No Hit
CTCGGATACTGCCCGCTGCCACTGCTCCTGCTCCTGCTGCTGCTCCTGCT13760.1413008095016579No Hit
GACAACGTCACTGAAAAGCGATGTTCTTTGGACTTCTGCTTTCGCCTCTC13470.13832281278977704No Hit
TGTCAGGGGAGAAGGGGGAGAAGCTCGGATACTGCCCGCTGCCACTGCTC13350.1370905382883091No Hit
CTCCCCTGACAACGTCACTGAAAAGCGATGTTCTTTGGACTTCTGCTTTC13280.13637171149578614No Hit
CGTCACTGAAAAGCGATGTTCTTTGGACTTCTGCTTTCGCCTCTCCTCAC13080.13431792066000622No Hit
GCGAAAGCAGGCCAGCTTGCCGGAGTTCGTCTCCAAACGGAATTTCTGAA13040.13390716249285026No Hit
AAGCAGGGGAGTCCAAGGGATGTAACTGCCCCGAGAGGCCTCAAACTGGC12970.13318833570032726No Hit
CCAAAGAACATCGCTTTTCAGTGACGTTGTCAGGGGAGAAGGGGGAGAAG12960.1330856461585383No Hit
GCGATGTTCTTTGGACTTCTGCTTTCGCCTCTCCTCACAAACACGGGAGA12900.1324695089078043No Hit
GGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAGTTCTGGATG12810.13154530303170334No Hit
CTGCATCTCGGATCGTGATTCCTCATTTAGAAGGAAGAACCAAATCTTGT12740.13082647623918037No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA12680.1302103389884464No Hit
GCCGGAGTTCGTCTCCAAACGGAATTTCTGAATGTACTGCCCCAGCCCTG12660.1300049599048684No Hit
CTTTGGACTTCTGCTTTCGCCTCTCCTCACAAACACGGGAGAGGCTGCAC12570.12908075402876745No Hit
TCTCCCCCTTCTCCCCTGACAACGTCACTGAAAAGCGATGTTCTTTGGAC12520.12856730631982247No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC12460.1279511690690885No Hit
GAGCAGCAGCAGGAGCAGGAGCAGTGGCAGCGGGCAGTATCCGAGCTTCT12450.12784847952729952No Hit
GAACATCGCTTTTCAGTGACGTTGTCAGGGGAGAAGGGGGAGAAGCTCGG12000.1232274501467947No Hit
GCGAAAGCAGGAGCAGTGGCAGCAGCAGGAGCAGCAGCAGGAGCAGCAGC11440.11747683580661095No Hit
GTCCAAGGGATGTAACTGCCCCGAGAGGCCTCAAACTGGCCCTTTCGTGG11430.11737414626482195No Hit
AAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAGTTCT11420.11727145672303296No Hit
GTCAGGGGAGAAGGGGGAGAAGCTCGGATACTGCCCGCTGCCACTGCTCC11410.11716876718124397No Hit
TCTCCAAACGGAATTTCTGAATGTACTGCCCCAGCCCTGCATCTCGGATC11340.116449940388721No Hit
CAGTGGCAGCGGGCAGTATCCGAGCTTCTCCCCCTTCTCCCCTGACAACG11330.11634725084693201No Hit
GGGCTGGGGCAGTACATTCAGAAATTCCGTTTGGAGACGAACTCCGGCAA11240.11542304497083103No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG11200.11501228680367505No Hit
GTTCGTCTCCAAACGGAATTTCTGAATGTACTGCCCCAGCCCTGCATCTC11060.11357463321862912No Hit
AGGCTGCACATCTTTTGGACAAGATTTGGTTCTTCCTTCTAAATGAGGAA11040.11336925413505113No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT10820.11111008421569321No Hit
ATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAACTACTAGTAT10690.10977512017243628No Hit
TGATTCCTCATTTAGAAGGAAGAACCAAATCTTGTCCAAAAGATGTGCAG10580.10864553521275733No Hit
TTTCTGAATGTACTGCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATT10450.1073105711695004No Hit
GAATGTACTGCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAGAA10440.10720788162771137No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG10420.1070025025441334No Hit
ATGTACTGCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAGAAGG10370.10648905483518843No Hit
TCAGTGACGTTGTCAGGGGAGAAGGGGGAGAAGCTCGGATACTGCCCGCT10360.10638636529339941No Hit
CAGCAGGAGCAGCAGCAGGAGCAGCAGCAGGAGCAGGAGCAGTGGCAGCG10310.10587291758445444No Hit
ACCAAAATGAAGGCAATACTAGTAGTTCTGGATGTGCTCTAATGGGTCTC10220.10494871170835349No Hit
AGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTA10190.1046406430829865No Hit
AAAGCAGGCCAGCTTGCCGGAGTTCGTCTCCAAACGGAATTTCTGAATGT10170.1044352639994085No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGA10120.10392181629046353No Hit
GGAAGAACCAAATCTTGTCCAAAAGATGTGCAGCCTCTCCCGTGTTTGTG10100.10371643720688555No Hit
AAATAAAAACAACCAAAATGAAGGCAATACTAGTAGTTCTGGATGTGCTC9990.1025868522472066No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT9940.10207340453826162No Hit
ATCTTGTCCAAAAGATGTGCAGCCTCTCCCGTGTTTGTGAGGAGAGGCGA9940.10207340453826162No Hit
AGCGAAAGCAGGGCTGGGACATCGAGCTGGCCGAGGGCGACGACCTGACG9760.10022499278605969No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)