FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A6a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A6a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1558239
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCAGGACAGCAATTGGAGAAGACAAGACCAGCAGCAGGCTTGGGGACA48890.3137516131992589No Hit
CTGTAGAGGCACCGCCTATCCAAGCCCCCATCCTAGGCCTGGAGGGGAAC40880.26234743194079985No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT36210.2323777032919854No Hit
TCCCAGAGGAGTCCAGGGCCAAGGGAAAGTGAGACTGGAGCATCTGAGAG35620.22859137783100025No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA33500.21498627617457913No Hit
GTCCAGGGCCAAGGGAAAGTGAGACTGGAGCATCTGAGAGAAAGTAGGAG32640.2094672255026347No Hit
CTCTAGGCCTCAGCATAGTCTGGGTGTCCCCAAGCCTGCTGCTGGTCTTG31600.20279302469005078No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT30830.1978515490884261No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT27740.17802147167411417No Hit
GCCTTATCCCTGTGGTCACACCTGTGATGCAGGGAGGTGGCTGAGTGGTG27630.1773155465881678No Hit
GTCCTGCACCACTCAGCCACCTCCCTGCATCACAGGTGTGACCACAGGGA27460.17622457145534157No Hit
GGCCTAGAGAGGCTGGGCTCATTGCTGGGAACCCAACCAAAGGGGCTGGT27430.17603204643190165No Hit
GGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCT26580.17057717076777054No Hit
GTCTGGGTGTCCCCAAGCCTGCTGCTGGTCTTGTCTTCTCCAATTGCTGT24990.1603733445254547No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACG24580.1577421692051091No Hit
CCTCTACAGCGTCCCAAGACTGAATATCCAGGTAGAGCCTCCTACTTTCT24580.1577421692051091No Hit
AACCAAGGGTGTCCAGGCAAGACAGGGAGGTGGCATGGGAAGGGTGGAAT23200.14888601812687272No Hit
GATTGAAAGCGACAGAGAATAGTTTCGAACAAATAACATTTATGCAAGCC22850.14663989285340695No Hit
CACCCAGACTATGCTGAGGCCTAGAGAGGCTGGGCTCATTGCTGGGAACC22820.14644736782996703No Hit
GTCTTGGGACGCTGTAGAGGCACCGCCTATCCAAGCCCCCATCCTAGGCC22130.14201929229084884No Hit
TCCTAGGCCTGGAGGGGAACATGACCAGCCCCTTTGGTTGGGTTCCCAGC22110.14189094227522223No Hit
GTGTATGAGGGGGTACTCCAATGCACCGTCAACCCTGTCCTTGGTATTTA21530.1381687918220504No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT21440.13759121675173064No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA21190.13598684155639798No Hit
TGCTTAGCCTTATCCCTGTGGTCACACCTGTGATGCAGGGAGGTGGCTGA20940.13438246636106527No Hit
GAAATAAGGTGGTTAATTGAAGAAATGCGGCACAGATTGAAAGCGACAGA20450.13123789097821323No Hit
GTTTGGGTGTGCCAGACTCAAACCAAGGGTGTCCAGGCAAGACAGGGAGG19810.12713069047816156No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTC19620.12591136532970873No Hit
GTAGAGGCACCGCCTATCCAAGCCCCCATCCTAGGCCTGGAGGGGAACAT19010.121996689853097No Hit
ACCCTGGACTTGCCAGACTACTGCTAAGTTTTTTGTTTGTTTGTTTCAAG18200.11679851422021911No Hit
GAATAGTTTCGAACAAATAACATTTATGCAAGCCTTACAACTACTGCTTG18100.11615676414208603No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA17680.11346141381392712No Hit
GTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACGATCAGTAATCC17390.11160033858734122No Hit
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTTC17270.11083023849358155No Hit
ACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACC16930.1086482882279291No Hit
TCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTCTACTTCAA16700.10717226304822303No Hit
GTCCAGGGTTGGTCCCTTACTCGACCCTTCGAACATTTAAGTACAGGAAG16500.1058887628919569No Hit
GCTCCAGACCATCCCGGCTAACAGGGTGAAAGCCCGTCTCTAGGAAAAAT16490.10582458788414358No Hit
GATCAGGAAGGGATGACATGCTTAGCCTTATCCCTGTGGTCACACCTGTG16390.1051828378060105No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC15700.10075476226689231No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)