FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3525513
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGGGAGGAGCCAGCCTGTGGGGCAGGGAGAGGAGGGCTGCAGCACC184090.522165143058613No Hit
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG141990.40274989767446606No Hit
CTACTGGGCTGTGGCTCCTTCTCCCCCTGGAGAATGGAGGGCCGGGTGAT110070.31220988264686583No Hit
TCCCTAGGCTGTGTAATTTAAGCCACAGCCCCACCTTCCCCATATCATGT105450.29910540678760794No Hit
CCCTAGGCCCAGGGGTTCCTGGTCTGTAAATGGGGAGAGACGGCCTGCAC105020.29788572613404063No Hit
GTGCAGTGCTGGGCAGGGGGTGAACACTCCCTGTTCCTGGGTCTTCTCCC90140.2556791025873398No Hit
GTACCAGGGATGTGAGCAGAAATGACAACGGCTTTCTAAGTGCAAGGCAA79490.2254707329117777No Hit
GGCCTAGGGAGCGTGTGTGTCTAGAGTGTGAATGGATCACAGATGAATGA74020.20995526041174714No Hit
GCACTGCACTGAGCGTTTTGTGGATTGTCTCATTCAGTGTTCACCAGTCC71970.204140503807531No Hit
GTACTATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAG69090.1959714798952663No Hit
ATACAGGTCAGTGTGAATGACATCCGAATTATATTAGTGGAAAAACTTGT66520.18868176064022454No Hit
CTCTAGACACACACGCTCCCTAGGCCCAGGGGTTCCTGGTCTGTAAATGG61490.1744143334601234No Hit
GGTCAGGAGTGGGGACTTTATTCAACATGTGATGGGAAGCCTGGGGAAGG56270.15960797761914364No Hit
CTGTGAGACCTGCCTGGCCCTGCTTTTGGTCTTATTAACCCACTCAGTTC55660.1578777329710598No Hit
GTGTGTATGTGTGCATGTGGGTGTGTAAACTGCTTTCCCAATACCAGTAG54470.15450233767397822No Hit
GTCTCACAGTGAGCAGACTGGCCTGGTGGAACTTGGAACCAACTAGAGAT54440.15441724367489215No Hit
GTGCTGGGCAGGGGGTGAACACTCCCTGTTCCTGGGTCTTCTCCCTCCAA51870.1471275244198504No Hit
GTCTAAGGGTTGACTTTTCTACAATAAGCTGCCCGATTTAATCAAGAGGA49620.1407454744883936No Hit
CCCTGGTACCTAGCAAAATGCCATGCGCACTAAATGGCCAGGATGACTCA49060.1391570531721199No Hit
GTCCTTTAGTTCTCCCAACAATGCTGCAGTGCAGGCCGTCTCTCCCCATT44820.12713043463461912No Hit
CTTTAAGCCCAGGCGTGCCGTGGTCTGATCTATGGATTTAAAAATCCTCC44520.1262794946437582No Hit
TGTCTAGAGTGTGAATGGATCACAGATGAATGAACACAGAGCCCATGGGT43710.12398195666843377No Hit
CCCATGGGCTCTGTGTTCATTCATCTGTGATCCATTCACACTCTAGACAC42960.12185460669128152No Hit
TCCCAGGACCCTGGGATCATGACCTGAGCCAAAGATAGATGCTTAATGAC42150.11955706871595707No Hit
AAACTATACAGGTCAGTGTGAATGACATCCGAATTATATTAGTGGAAAAA41410.11745808340516686No Hit
GGGTAAAGGCTTGGGGCCCAAGGGTAGAGGAAAGATGGCTCTGAAACCAC40890.11598312075434128No Hit
GCCCAGCACTGCACTGAGCGTTTTGTGGATTGTCTCATTCAGTGTTCACC40650.11530236876165255No Hit
GTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATGAACAAGGAATAACC39940.11328847744994841No Hit
GTGTTTGAGTCATCCTGGCCATTTAGTGCGCATGGCATTTTGCTAGGTAC39670.11252263145817361No Hit
GCCCATGGGTCCTCATTTCCACTTGGTCCCAACTGGCTGAGGGGTGGCTG39140.11101930414098601No Hit
ATATGGGGAAGGTGGGGCTGTGGCTTAAATTACACAGCCTAGGGAGGAGC38670.10968616482197059No Hit
GCTCTGGACACTACTGGGCTGTGGCTCCTTCTCCCCCTGGAGAATGGAGG38570.10940251815835028No Hit
GTGTGAATGACATCCGAATTATATTAGTGGAAAAACTTGTTCCTACACTC37720.10699152151757772No Hit
ACATTATGGTAAGGGCTCTAGCTTACTGCTGCCTTCCTAATGGCTTAATA36870.10458052487680516No Hit
CCTCTAAGGCAGCTTCCTACTATGGGGGTTGTTTCCCATTTAGGGACCAG36860.10455216021044314No Hit
GTATAATTTAGTATTTCAGACCCAATCTTCTCCCCAACCCTGCAAAGGAA36420.10330411489051379No Hit
GCCCTGAGGACGTCAGGAGTGAAGCACATGATATGGGGAAGGTGGGGCTG35890.1018007875733262No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)